Fluigent OMI User Manual

Fluigent OMI User Manual

Single channel platform

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USER'S
MANUAL

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Summary of Contents for Fluigent OMI

  • Page 1 USER’S MANUAL...
  • Page 2: What Is Included

    O-OMI-HRCC *Comes in sterile package *Comes in sterile package (3) Cartridge (1) Power supply kit* (1) Air filter & pneumatic kit (1) OMI connector & tubing kit O-OMI-CART O-OMI-SK O-OMIAF-CTK O-OMI-CTK *Comes in sterile package *Socket depending on the region...
  • Page 3 10 different solutions at the desired flow rate into a microfluidic Pipetting solution chip, perfusion chamber or petri dish. Prevent any objects or liquid from entering the Omi device, this may Pneumatic filter / External gas source cause a short-circuit failure or other malfunction. Failing to respect...
  • Page 4 Omi can be used in single or dual perfusion mode to perform either single channel perfusion for blood vessel reproduction for example Membrane Cells (type 1) or co culture for liquid/liquid interfaces for gut on chip or blood brain Channel 1 barrier reproduction by synchronizing two Omis with one organ on chip.
  • Page 5: Hardware Preparation

    Some places also ask for a MAC-based recognition of the devices 10 different solutions at the desired flow rate into a microfluidic To power Omi OFF, press the button for a few seconds. The screen will connected to a Wifi network to allow their access to the internet. Check chip, perfusion chamber or petri dish.
  • Page 6 The low resistance adaptor allows to reach up to 1ml/min flow rate with Omi (without taking the chip design and resistance into account). High resistance adaptor can be used for experiment where low Cartridge holes flow rate is needed.
  • Page 7 When the cartridge is in place, the adaptor can now be positioned. Pipetting solutions in your cartridge The adaptor allows the Omi to be compatible with both low and high To fill the reservoir with a solution once the cartridge is inserted, resistance Organ-on-chip chips for better versatility.
  • Page 8: Application Configuration

    All Omi users are part of Installation a team. Within this team, Admin devices, layouts and protocols The Omi mobile application is available via the Google Play Store of will shared allow any Android-based tablet. To find it, simply search for “Omi”, access different members of the the application page, check that the publisher is “Fluigent SAS”...
  • Page 9 Omi website (https://omi.fluigent.com). To register you will need one of two pieces of information: The serial number of one of your Omi device, can be found on the tag below the device: On the registration page, fill in the required information.
  • Page 10 SETTING UP Launch Omi service web First login - tutorial When first logging in to the Omi mobile application, users will be Open in a browser https://omi.fluigent.com. On the login page (see below), click on “Register”. guided through a 4-step tutorial in order to run your first protocols.
  • Page 11 As explained in Power ON, the page displays some explanations on devices previously, all team devices will appear here. how to turn ON your Omi devices. It also illustrates how to get the Bluetooth address of all your devices via the embedded screen.
  • Page 12 A chip can possess 1 or 2 channels and will therefore be use of this layout. A popup will notify you that going forward will close associated by either a single Omi device or two. Associating 2 devices the tutorial.
  • Page 13 SETTING UP SETTING UP Start protocol Visualization of layout - Home page Device information page Upon starting the application, or after finalizing the tutorial, you will be brought to the home page. On this page, all devices of the current layout are visible.
  • Page 14 SETTING UP SETTING UP Protocol editor Single perfusion mode When accessing the protocol editor, a configuration window asks whether to load an existing protocol or create a new one. If you’ve selected to add pre- and post-processing steps in the To create a new protocol, some information must be provided: configuration window, they will appear.
  • Page 15 SETTING UP SETTING UP The top bar provides some information about the current protocol This window allows you to tune the step parameters: and some basic controls/ From left to right: Name - can be changed via the editing icon at the right of the field. Control type (Flowrate &...
  • Page 16 They are usually divided in several sub-steps that require either a confirmation from the user, or an action with the Omi device. The Once finished, save your protocol and press the top right button to pre-load the blocking steps are: Calibration, Sterilization, Loading and Cleaning.
  • Page 17 Pressing the button will trigger the synchronization between either 1) the Omi internal memory and the tablet storage or 2) the tablet storage and the remote server database.
  • Page 18: Warning List

    Omi automatically stores data every 30 secondes during an experiment. The storage reduced pressure and flow-rate no space exist between the capacity is up to 10 days of data.
  • Page 19: Error List

    Splash screen that no space exist between them At startup, a splash screen with the Omi logo is displayed for about Unreachable The requested flow-rate cannot be Check that the pressure 5 seconds.
  • Page 20: Main Screen

    EMBEDDED DISPLAY EMBEDDED DISPLAY Main screen When and experiment is running, the bottom navigation bar indicates : The first page to The measured flowrate value be displayed is the The computed shear stress value main screen which The name of the running step and its position in the experiment indicates the state of The step percentage of completion the device.
  • Page 21 EMBEDDED DISPLAY EMBEDDED DISPLAY Warning / Error screen Pause / Stop screen This screen is made of two tabs, one for the warnings and one for the errors. A warning is a non critical issue that does not prevent the system normal operation.
  • Page 22 EMBEDDED DISPLAY EMBEDDED DISPLAY Resume / Stop screen Experiment summary screen This pause / stop screen will be displayed if an experiment is paused The experiment summary screen lists all the steps of the running and the play button is pressed from the Main screen. experiment.
  • Page 23: Update Screen

    EMBEDDED DISPLAY EMBEDDED DISPLAY Update screen Menu screen The menu screen allows for If a new update is available, this navigation through the embedded screen allows you to start the screen interface. installation process. Press the manual mode button to go to Manual mode screen and start functions such as injection, sampling or recirculation without using the mobile app.
  • Page 24: Advanced Settings Screen

    EMBEDDED DISPLAY EMBEDDED DISPLAY Advanced settings screen Manual mode screen The Advanced settings screen allows you to : The manual mode screen allows users to start manually and without using the mobile app the following functions : Injection (from I to RO) Perfusion (from RI to RO) Sampling (from RI to O) Recirculation (from RO to RI)
  • Page 25: Hardware Specifications

    Arrange a mutually convenient time for Fluigent service representative to discuss and find a solution to fix the issue. Repairs will be made remotely whenever possible. If more action is needed, the system will need to be sent back to Fluigent Still have a question? offices (for no additional cost, only if it is under warranty).
  • Page 26: Safety & Information

    - E-mail: contact@fluigent.com FCC warning hereby declares that this [Name: OMI, Model: OMI] is in compliance with the This device complies with Part 15 of the FCC Rules. Operation is subject to the essential requirements and other relevant provisions of Directive 2014/53/EU.
  • Page 27 NOTES CE warning Adapter shall be installed near the equipment and shall be easily accessible. Only can use adapter as below: - Power supply Type: 3721-24 - Input: 100-240Vac 50-60Hz max 1.0A; - Output: 24Vdc 1.75A 42W - Mascot Electronics AS The plug considered as disconnect device of adapter.
  • Page 29 VERSION MAR. 2023...

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