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Media Wand™ and Media
Handling Unit
Operating Instructions
Original instructions
cytiva.com

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Summary of Contents for cytiva Media Wand

  • Page 1 Media Wand™ and Media Handling Unit Operating Instructions Original instructions cytiva.com...
  • Page 2: Table Of Contents

    Adjust resin concentration and prepare a slurry ................42 Use of Media Wand with Chromaflow Packing Station 100 ............47 5.6.1 Connect a Media Wand to a Chromaflow Packing Station 100 ..........48 5.6.2 Prime the Chromaflow Packing Station 100 and Media Wand ..........50 5.6.3...
  • Page 3 European Union and European Economic Area ................83 8.5.3 Great Britain ..............................84 8.5.4 Other regulations and standards ......................85 Ordering information ........................... 86 Health and Safety Declaration Form ...................... 87 Index ........................... 89 Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 4: Introduction

    1 Introduction Introduction About this chapter This chapter contains important user information, descriptions of safety notices and intended use of the Media Wand™ and the Media Handling Unit. In this chapter Section See page Important user information About this manual...
  • Page 5: Important User Information

    Intended use The Media Wand and Media Handling Unit are intended for use in the preparation and handling of bulk chromatography resin solutions for the separation of biomolecules such as proteins, peptides and oligonucleotides in GMP-regulated environments.
  • Page 6 • The products must be installed according to the instructions in Chapter 4 Installa- tion, on page 26 of these Operating Instructions. • The user must be trained in handling chromatography resin in general. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 7: About This Manual

    The Operating Instructions manual provides information needed to install, operate and maintain the product in a safe way. Scope of this manual The Operating Instructions cover the Media Wand and Media Handling Unit. Typographical conventions Software items are identified in the text by bold italic text.
  • Page 8: Abbreviations

    Personal Protective Equip- Personal Protective Equip- ment ment TC-clamp hygienic tubing connection hygienic tubing connection Water For Injection Water For Injection Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 9: Safety Instructions

    All users must read and understand the entire contents of this general safety chapter, and the specific safety precau- tions information in each subsequent chapter of this manual to become aware of the hazards involved. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 10: Safety Precautions

    It is important not to proceed until all stated conditions are met and clearly understood. NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 11 A sudden pressure increase may cause rupture of piping connections and consequent leakage. Personal protection WARNING Always use appropriate Personal Protective Equipment (PPE) during operation and maintenance of this product. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 12 Explosive environment. The product is not approved for work in a potentially explosive atmosphere. WARNING Some of the chemicals used with Media Wand and Media Handling Unit may be flammable under certain conditions. Make sure to use chemicals only under conditions where they are not flammable.
  • Page 13: Labels And Symbols

    Instrument serial number Year of manufacturing Manufacturing year Operating temperature TS Operating temperature range Supply air pressure Supply air pressure requirements Air consumption Instrument air consumption Pump capacity Instrument pump capacity Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 14: Emergency Procedures

    2.3 Emergency procedures Emergency procedures Introduction This section describes emergency shutdown of the Media Wand and Media Handling Unit in an emergency situation, and the procedure for restarting the Media Wand and Media Handling Unit. Precautions WARNING Pressure may remain in the tubing for an extended period after shutdown.
  • Page 15 Set the valve control switches to 0. Press the RESET button. Note: The pump restarts immediately when the valves are re-opened. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 16: System Description

    3 System description System description About this chapter This chapter gives an overview of the Media Wand and the Media Handling Unit, and a brief description of its function. In this chapter Section See page Media Wand and Media Handling Unit...
  • Page 17: Media Wand And Media Handling Unit

    3.1 Media Wand and Media Handling Unit Media Wand and Media Handling Unit Introduction The Media Wand and the Media Handling Unit are designed to facilitate the handling of resin in containers. Illustration of Media Wand and Media Handling Unit...
  • Page 18 Media Wand 100 (Resin Wand) Control panel EMERGENCY STOP RESET Pump Hose connections Description of connection points Part Description Transfer to waste Wand spray Wand suction Transfer to slurry tank Buffer in Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 19 Example: External pressure (air pressure) is 4 bar g. To obtain a pump flow of 40 L/min, the pump pressure is set to 3 bar g, which results in an air consumption of 0.3 m /min. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 20: Control Panel

    3 System description 3.2 Control panel Control panel Control panel overview The Media Wand and Media Handling Unit are controlled from the control panel. The control panel is located on top of the Media Handling Unit illustrated below. Part Description...
  • Page 21 TRANSFER TO. The following choices are available: • SLURRY TANK directs the flow to the slurry tank. • WASTE OUTLET directs the flow to the waste container. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 22: Media Wand

    3 System description 3.3 Media Wand Media Wand Media Wand overview Part Description Spray inlet Suction outlet Suction inlet Spray nozzle tip Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 23 Spray inlet Rubber gasket Inner pipe The inner and outer pipes form the Media Wand. The PEEK nozzle and spray inlet are on the inner pipe. The suction outlet is on the outer pipe. A TC-clamp joins the two pipes together.
  • Page 24 Large decanting plate How the Media Wand functions A buffer is sprayed through the Media Wand spray nozzle. This agitates and mixes the sedimented resin in the transport container. Recirculation of the slurry agitates the sedimented resin to a highly concentrated slurry suitable for column packing.
  • Page 25 3 System description 3.3 Media Wand Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 26: Installation

    4 Installation Installation About this chapter This chapter provides required information to enable users and service personnel to unpack, install, move and transport the Media Wand and Media Handling Unit. In this chapter Section See page Safety precautions Site requirements...
  • Page 27: Safety Precautions

    4.1 Safety precautions Safety precautions Installing and moving the instrument CAUTION Heavy object. Use suitable lifting equipment when moving the product. Three people are required to manually lift the product safely. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 28: Site Requirements

    • The instrument should not be exposed to sources of heat, such as direct sunlight • Dust in the atmosphere should be kept to a minimum • The equipment must not be exposed to vibrations Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 29: Transport

    The Media Handling Unit is equipped with wheels that facilitate movement. The unit can be pushed into position as long as the floor surface is level and smooth. Always lock the wheels when using the unit. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 30: Unpacking

    4 Installation 4.4 Unpacking Unpacking Unpacking overview • Check the equipment for any visible damage before starting installation. • Document and photograph any damage carefully and notify your Cytiva represen- tative. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 31: Assembly

    Set all three valve control switches to 0. Set the pump switch to OFF and the pump pressure to minimum. Connect the air supply to the air supply port located at the bottom of the cabinet. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 32 Follow the steps below to connect hoses to the Media Wand. Step Action Connect hoses to the Suction and Spray ports of the Media Wand. Use gaskets and fasten with TC-clamps. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 33: Operation

    5 Operation Operation About this chapter This chapter gives instructions on how to operate the Media Wand and Media Handling Unit in safe way. In this chapter Section See page Safety precautions Prime the Media Wand and Media Handling Unit...
  • Page 34: Safety Precautions

    5 Operation 5.1 Safety precautions Safety precautions Operation WARNING To reduce the risk of static electricity, use hoses with conductivity characteristics. Recommended length is 2 m. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 35: Prime The Media Wand And Media Handling Unit

    Prime the Media Wand and Media Handling Unit Procedure Before using the Media Wand and Media Handling Unit, all inlets and outlets must be primed as described in this section. Follow the steps below to prime the Media Wand and Media Handling Unit.
  • Page 36 Prime for 30 seconds. Set the valve control switches on the control panel to the following posi- tions: • INLET: WAND SUCTION • OUTLET: TRANSFER TO • TRANSFER CONTROL: SLURRY TANK Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 37 5 Operation 5.2 Prime the Media Wand and Media Handling Unit Step Action Prime for 30 seconds. Stop the pump. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 38: Check The Flow Of The Media Handling Unit

    Make sure the EMERGENCY STOP is reset. Set the valve control switches on the control panel to the following posi- tions: • INLET: WAND SUCTION • OUTLET: TRANSFER TO • TRANSFER CONTROL: WASTE OUTLET Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 39 3 bar g. Fill the container for a set time. Weigh the container and calculate the obtained flow. Repeat the procedure with an adjusted pump pressure until a flow of 40 ± 2 L/min. has been obtained. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 40: Remove Transport Solution From A Resin Container

    Position the Media Wand above the liquid surface. Start the pump with a minimum of pump pressure. Adjust to approximately 2 bar g. Make sure that the pump is running. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 41 The spray nozzle should be positioned just above the resin surface. Keep the Media Wand in a position just below the liquid surface while aspi- rating off the transport solution. The Media Wand can occasionally be lifted above the liquid surface without disrupting the process. Remove as much of the transport solution as possible without removing any resin.
  • Page 42: Adjust Resin Concentration And Prepare A Slurry

    The preparation of the slurry starts with the addition of buffer to obtain an appropriate resin concentration. As the Media Wand is lowered during the addition of buffer, the preparation of the slurry is initiated. When all the buffer has been added, the recircula- tion of resin and buffer starts and the slurry preparation continues.
  • Page 43 5.5 Adjust resin concentration and prepare a slurry Step Action Place the Media Wand in the resin container. The spray nozzle should be positioned just above the resin surface. Start the pump with a flow of 40 ± 2 L/min.
  • Page 44 5.5 Adjust resin concentration and prepare a slurry Step Action Continue to move the Media Wand slowly up, down and around the container walls until all resin is in slurry suspension. NOTICE To maintain integrity of the resin, never exceed the maximum time of ~5 minutes (60 L resin) or ~2 minutes (10 L) of recirculation while resuspending resin.
  • Page 45 Start the pump with a flow of 40 ±2 L/min. When the slurry level in the container is less than 5 cm (2 inches) from the bottom, tilt the container and place the Media Wand at the lowest point in the container. Remove as much slurry as possible.
  • Page 46 When the slurry level in the container is less than 5 cm (2 inches) from the bottom, tilt the container and place the Media Wand at the lowest point in the container. Remove as much of the slurry as possible.
  • Page 47: Use Of Media Wand With Chromaflow Packing Station 100

    5.6 Use of Media Wand with Chromaflow Packing Station 100 Use of Media Wand with Chromaflow Packing Station Introduction This section describes how to operate the Media Wand with a Chromaflow Packing Station 100. For detailed information on the handling of the Chromaflow Packing Station 100, refer to Operating Instruction 29046228.
  • Page 48: 5.6.1 Connect A Media Wand To A Chromaflow Packing Station 100

    Wand spray Transfer to waste/slurry tank To facilitate the handling of the Media Wand, a 90º connection at position B and SIT can be used. The hose from the SIB outlet is used for both transfer to waste and slurry tank.
  • Page 49 5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.1 Connect a Media Wand to a Chromaflow Packing Station 100 Step Action Set the PACKING PUMP and UNPACKING PUMP air pressure to 0. Set the NOZZLE CONTROL to 0.
  • Page 50: Prime The Chromaflow Packing Station 100 And Media Wand

    Action Fill a container with packing buffer or WFI or other suitable liquid to be used for suspension of the resin, and place the Media Wand and all hoses into the container. Set the valve control switches on the control panel to the following posi- tions: •...
  • Page 51 5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.2 Prime the Chromaflow Packing Station 100 and Media Wand Step Action Start the pump with the FINE ADJUSTMENT regulator by setting the pump pressure to 3 bar g.
  • Page 52: Check The Flow Of The Chromaflow Packing Station 100

    5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.3 Check the flow of the Chromaflow Packing Station 100 5.6.3 Check the flow of the Chromaflow Packing Station 100 Procedure The recommended flow when preparing resin slurry is 40 ± 2 L/min. A pump pressure of approximately 3 bar g will usually produce this flow.
  • Page 53 5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.3 Check the flow of the Chromaflow Packing Station 100 Step Action Weigh the container and calculate the resulting flow. Repeat the procedure with an adjusted pump pressure until a flow of 40 ±...
  • Page 54: Remove The Transport Solution From A Resin Container

    5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.4 Remove the transport solution from a resin container 5.6.4 Remove the transport solution from a resin container Procedure Resin is delivered in a transport solution, usually 20% ethanol. Follow the steps below to remove the transport solution from a resin container.
  • Page 55 Remove as much of the transport solution as possible without removing any resin. Lift the Media Wand out of the liquid while the pump is still running. Turn off the pump. If a decanting device has been used, replace it with a spray tip.
  • Page 56: Prepare The Slurry For Use

    50%. Make sure: • the system is primed and a spray tip is fitted to the Media Wand. • there is a hose connected from position SIB to a slurry tank. • there is buffer connected to the Chromaflow Packing Station 100 position C (BUFFER IN position).
  • Page 57 The spray nozzle should be positioned just above the resin surface. Place the Media Wand in the resin container. The spray nozzle must be posi- tioned just above the resin surface. Start the pump with a flow of 40 ± 2 L/min.
  • Page 58 • Inlet to B • Outlet to SIT Continue to move the Media Wand slowly up, down and around the container walls until all resin is in slurry suspension. NOTICE To maintain integrity of resin, never exceed the maximum time of ~5 minutes (60 L resin) or ~2 minutes (10 L).
  • Page 59 5 Operation 5.6 Use of Media Wand with Chromaflow Packing Station 100 5.6.5 Prepare the slurry for use Step Action Stop the pump and then immediately continue with Transfer initial bulk resin to slurry vessel, on page NOTICE Do not leave resin residues in the system. Do not shut...
  • Page 60 5.6.5 Prepare the slurry for use Step Action Tilt the container and place the Media Wand in the bottommost location when the slurry level in the container is less than 5 cm (2 inches) from the bottom. Remove as much slurry as possible.
  • Page 61 • Outlet to SIB Start the pump. Tilt the container and place the Media Wand in the bottommost location when the slurry level in the container is less than 5 cm (2 inches) from the bottom. Remove as much slurry as possible.
  • Page 62: Procedures After A Run

    Procedures after a run Rinse the system Immediately after the resin transfer procedure, the system must be rinsed. See Section 6.4 Rinse the Media Wand and Media Handling Unit with water, on page 67 for a description of the procedure. NOTICE Do not leave resin residues in the system.
  • Page 63: Maintenance

    See page Safety precautions Cleaning before planned service User maintenance schedule Rinse the Media Wand and Media Handling Unit with water Clean the Media Wand Clean the Media Handling Unit Storage Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 64: Safety Precautions

    Cytiva only. CAUTION Heavy object. Use suitable lifting equipment when moving the product. Three people are required to manually lift the product safely. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 65: Cleaning Before Planned Service

    Health and safety declaration forms Health and safety declaration forms are available for copying or printing in the Refer- ence information chapter of this manual, or on digital media supplied with the user documentation. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 66: User Maintenance Schedule

    Introduction The maintenance recommendations are different depending on the specific applica- tion and frequency of use of Media Wand and Media Handling Unit. Note that the recommendations may not apply to your specific use of Media Wand and Media Handling Unit. The owner has sole responsibility for establishing applicable routines for periodic maintenance.
  • Page 67: Rinse The Media Wand And Media Handling Unit With Water

    6 Maintenance 6.4 Rinse the Media Wand and Media Handling Unit with water Rinse the Media Wand and Media Handling Unit with water Procedure Follow the steps below to rinse the system with water. Step Action Place the buffer In hose in clean water.
  • Page 68 6 Maintenance 6.4 Rinse the Media Wand and Media Handling Unit with water Step Action Discard the water and repeat the procedure with clean water until the rinse water stays clear. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 69: Clean The Media Wand

    6.5 Clean the Media Wand Clean the Media Wand Procedure Remove the Media Wand from the unit. Clean the parts and/or clean the wand in an autoclave. WARNING Do not perform cleaning in place (CIP) on the Media Wand by pumping NaOH through the wand, due to the risk of NaOH spray.
  • Page 70: Clean The Media Handling Unit

    When the pH on the outlet side is equivalent to pH on the inlet side, stop the pump. Cleaning the Media Handling Unit with CIP manifold Two sizes of CIP manifold are available as accessories for the Media Handling Unit. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 71 Action Connect the CIP manifolds to the inlets and outlets, and place the uncon- nected ends in a 100 L tank with at least 20 L of 1 M NaOH. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 72 Continue pumping WFI through the pump and measure the pH of the outgoing flow. When the pH on the outlet side is equivalent to pH on the inlet side, stop the pump. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 73: Storage

    Storage recommendations The system should be filled with a 20% ethanol solution when in storage. Place the Media Wand and Media Handling Unit in a dust-free environment with well- controlled climate. The temperature must be stable and in the range 4°C to 25°C.
  • Page 74: Troubleshooting

    Make sure the PEEK nozzle is not damaged or loose. slurry formation Contamination In certain circumstances it may be necessary to change O- rings, gaskets and seals in wetted parts. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 75: Reference Information

    8 Reference information Reference information About this chapter This chapter lists the technical specifications of the Media Wand and Media Handling Unit. The chapter also includes a chemical resistance guide, ordering information, and Health and Safety Declaration forms for service.
  • Page 76: Media Wand Specifications

    • Media Wand 100, 1 m length, for large (60 L) resin containers. • Media Wand 50, 0.5 m length, for smaller (5 or 10 L) resin containers. For additional technical data refer to the product documentation binder provided with the product.
  • Page 77: Media Handling Unit Specifications

    70 kg Media Handling Unit air supply Characteristic Pneumatic requirement Air Supply 5 to 7 bar g Air consumption (estimated) 500 Nl/min/ 5 to 7 bar g Type Instrument air Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 78: Chemical Resistance

    Cytiva representative for recommendations. Chemicals The Media Wand and Media Handling Unit are resistant to chemical agents commonly used in protein recovery, including buffer solutions for adsorption, elution and washing, and to solutions effective in cleaning, sanitization and storage up to 1 year.
  • Page 79 Note also that the combined effects of agents have not been taken into account in this table. The table in this chapter is a guide to the resistance of Media Wand and Media Handling Unit to chemicals and solvents commonly used in process chromatography.
  • Page 80: Recycling Information

    8 Reference information 8.4 Recycling information Recycling information Introduction This section contains information about the decommisioning of Media Wand and Media Handling Unit. Disposal of the product When taking the product out of service, the different materials must be separated and recycled according to national and local environmental regulations.
  • Page 81: Regulatory Information

    8 Reference information 8.5 Regulatory information Regulatory information Introduction This section lists the regulations and standards that apply to the Media Wand and Media Handling Unit. In this section Section See page 8.5.1 Contact information 8.5.2 European Union and European Economic Area 8.5.3...
  • Page 82: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 83: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 84: Great Britain

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 85: Other Regulations And Standards

    Other regulations and standards Additional design considerations For additional design considerations, such as ASME-BPE, GMP and cGMPs information refer to the product-specific specification information provided in the documentation package delivered with each equipment. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 86: Ordering Information

    Accessories and spare parts To get the latest information about accessories and user replaceable spare parts that are available for the Media Wand and the Media Handling Unit, see cytiva.com. Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 87: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 88 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.
  • Page 89: Index

    Preventive maintenance, 66 Priming, 35 Flow rate, 19, 38 Product return or servicing, 88 Pump, 19, 38 Purpose of this manual, 7 Important user information, 5 Inner and outer pipe, 23 Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 90 Storage, 73 start-up, 73 System nameplate, 13 description, 13 Transport, 29 Troubleshooting, 74 Typographical conventions, 7 UK, 84 conformity, 84 UKCA, 84 marking, 84 Unpacking, 30 Volume calculation, 42 Wheels, 29 Media Wand and Media Handling Unit Operating Instructions 28963868 AD...
  • Page 91 Page intentionally left blank...
  • Page 92 Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp. or an affiliate doing business as Cytiva. Chromaflow and Media Wand are trademarks of Global Life Sciences Solutions USA LLC or an affiliate doing business as Cytiva.

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