Knauer Azura pH 2.1S Instructions Manual
  1. Manuals
  2. Brands
  3. Knauer Manuals
  4. Measuring Instruments
  5. Azura pH 2.1S
  6. Instructions manual

Knauer Azura pH 2.1S Instructions Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
pH Monitor pH 2.1S
Instructions
HPLC
HPLC
Document No. V6733

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Azura pH 2.1S and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Knauer Azura pH 2.1S

Summary of Contents for Knauer Azura pH 2.1S

  • Page 1 pH Monitor pH 2.1S Instructions HPLC HPLC Document No. V6733...
  • Page 2 KNAUER. Support: Do you have questions about the installation or the operation of your instrument or software? International Support: Contact your local KNAUER partner for support: www.knauer.net/en/Support/Distributors-worldwide Support in Germany (Austria & Switzerland on case-to-case basis): Phone:...

  • Page 3: Table Of Contents

    Table of contents General ................1 About these instructions .
  • Page 4 4.1.1 Location requirements ........... . . 9 4.1.2 Unpacking .
  • Page 5 8.3.1 Cleaning pH electrode ........... .27 8.3.2 Cleaning pH flow cell .

  • Page 6: General

    These operating instructions are an integral part of the device. It must be kept in the immediate vicinity of the device and accessible to the user at all times. You can download these and other instructions from the KNAUER web- site: www.knauer.net/library 1.2 Signal words...

  • Page 7: Legal Information

    1.4.4 Warranty seal A blue or orange warranty seal is affixed to some devices. A blue seal is used by KNAUER’s Manufacturing or Customer Support ƒ for devices to be sold. After repair, service technicians attach an orange seal onto the identi- ƒ...

  • Page 8: Basic Safety Instructions

    Operation in potentially explosive areas without special and addition- ƒ al explosion protection. Contact the KNAUER Customer Support for more information. 2.2 User qualification The users are qualified to handle the device if all of the following points apply: They have at least a basic knowledge of liquid chromatography.

  • Page 9: Operator Responsibility

    ƒ alcohol or medication. They have participated in the installation of an instrument or training ƒ by KNAUER or an authorized company. If users do not meet these qualifications, they must inform their supervisors. 2.3 Operator responsibility The operator is any person who operates the device himself or leaves it to a third party for use and who bears the legal product responsibility for the protection of the user or third parties during operation.

  • Page 10: Explosive Environment

    Devices which are shipped without the completed document “Service request form and decontamination report” will not be repaired. If you would like to return a device to KNAUER, make sure to enclose the com- pleted document: www.knauer.net/servicerequest. AZURA® pH Monitor pH 2.1S Instructions, V6733...

  • Page 11: Product Information

    3. Product information 3.1 Features The AZURA pH 2.1S is monitoring the pH value of buffers in liquid chro- matography systems. It is used for the value read out from the electrode and subsequent data exchange with the chromatography software.

  • Page 12: Symbols And Signs

    3.3 Symbols and signs The following symbols and labels are located on the device: Symbol Description Electrostatic discharge hazard, damages to system, device, or components can occur. Hazard due to high voltage. A device or system marked with CE fulfills the product spe cific requirements of European directives.

  • Page 13: Eluents/Buffers

    Note: The list of selected solvents was compiled based on research in the perti nent literature and is only a recommendation by KNAUER. 3.5.1 Suitable eluents/buffers Eluents/buffers suitable for pH flow cell pH flow cell Aqueous buffer solutions (such as acetate, formiate, phosphate) ƒ...

  • Page 14: Installation

    Installation pH electrode Eluents/buffers unsuitable for pH electrode or glass electrodes in general Media that can dissolve the glass surface, for example: Hydrofluoric acid solutions ƒ Acidified fluoride solutions ƒ Concentrated alkalis ƒ Media that can dehydrate the glass surface, for example: Ethanol ƒ...

  • Page 15: Unpacking

    Installation 4.1.2 Unpacking Prerequisite There is no noticeable damage to the packaging. Utility knife Tools Process 1. Place the package in such a way that you can read the label. Using the utility knife, cut the adhesive tape and open the packaging. 2.

  • Page 16: Azura® Ph Sensor

    Installation 4.2 AZURA® pH sensor When connected to the pH 2.1S, the pH sensor is used to monitor the pH value of aqueous solutions such as buffers in flow. The pH sensor consists of a pH electrode and a pH flow cell. When flushing the pH sensor under non-compatible flow, pressure or sol- vent composition conditions, the pH electrode should be excluded from the flowpath (see...

  • Page 17: Assembling Ph Sensor And Attaching To Ph Monitor Azura® Ph 2.1S

    Installation Next steps Connect the AZURA® pH electrode to the AZURA® pH 2.1S and the AZURA® pH flow cell (see next chapter). 4.2.2 Assembling pH sensor and attaching to pH monitor AZURA® pH 2.1S Prerequisites The AZURA® pH 2.1S is switched off. ƒ...

  • Page 18: Connecting The Device To The Computer

    4.3.1 Connecting the device to the computer via LAN Note: HPLC devices made by KNAUER work only with IP addresses which are assigned via IPv4. IPv6 is not supported. This section describes how to set up an HPLC system in a local area network (LAN) and how a network administrator can integrate this LAN into your com pany network.

  • Page 19: Connecting Devices To The Lan

    Installation For all LAN devices: For the network adapter, the following option in ƒ the Device Manager must be deactivated: „Allow the computer to turn off this device to save power“. Process Procedure 1. In Windows, open <Network and Sharing Center>. 2.

  • Page 20: Configuring The Router

    4.3.4 Configuring the router The router is preset at the factory. You find information about IP address, user name and password in the router instructions: www.knauer.net/router Process 1. To open the router configuration, start your Internet browser and enter the IP address (not for all routers).

  • Page 21: Setting Ip Addresses Via Software

    Installation 4.4 Setting IP addresses via software Note: Check the IT security standards for your lab before intervening in the LAN settings. Static IP addresses are required to run certain chromatography software, PurityChrom® e.g. Purity Chrom®. For a comprehensive overview on how to set static IP addresses for PurityChrom®, refer to the document „PurityChrom®...

  • Page 22: Firmware Wizard: Setting A Dynamic Ip Address

    Installation Process 6. Click <Reset Conn. Settings> to accept changes. 7. Restart the device (recommended). Result The device is now accessible via the static IP address. 4.4.2 Firmware Wizard: Setting a dynamic IP address Prerequisites The device is switched on. ƒ...
  • Page 23 Installation Process Figure 1. Set the on/off switch to the OFF position 2. Connect the power supply to the device and afterwards plug the power plug into the socket. Next steps Set the on/off switch to the ON position and start up the device. AZURA®...

  • Page 24: Operation

    Operation 5. Operation The device is operated via the membrane keypad. The measurement can be followed via software, (e.g. PurityChrom® or ClarityChrom®) on the display. 5.1 Initial start-up In case the monitor is part of a FPLC/HPLC system, check for the following crite ria: A connection to the computer via the desired interface has been ƒ...

  • Page 25: Menu Items And Factory Settings

    Operation 5.2.2 Menu items and factory settings Display content Description Two menus help to calibrate the pH sensor: #0 is preset to pH07 ƒ #1 can be adjusted to a value between pH02 - ƒ pH12 and corresponds to the pH value of the second calibra tion solution that is used.

  • Page 26: Checking The Operating Hours

    Operation 5.4 Checking the operating hours The operating hours of the monitor can be viewed in the GLP menu, which dis plays the value in hours according to GLP specifications. Prerequisites The monitor has been switched on. Process Display 1. Press both arrow keys. 2.
  • Page 27 7. Re-integrate the calibrated AZURA® pH electrode into the AZURA® pH flow cell. 8. Flush the AZURA® pH sensor with solvent compatible for the AZURA® pH electrode immedeately after to prevent damaging the AZURA® pH electrode. Table 1 Process Display 1.

  • Page 28: Switching Off Device

    Operation 5.6 Switching off device Remove the pH electrode - if installed - before using salt-free solvents for rinsing. For this purpose, utilize the bypass of the AZURA® pH flow cell or the AZURA® pH dummy electrode to maintain system flow after removing the AZURA®...

  • Page 29: Functionality Tests

    6.1 Installation Qualification (IQ) The customer may request the Installation Qualification, which is free of charge. In case of a request, the technical support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation.

  • Page 30: Troubleshooting

    Troubleshooting 7. Troubleshooting If the device stops functioning or there are connection problems, start by check ing the following items: Does the display light up? ƒ Are the LEDs on the LAN port turned on? ƒ When you connect the cable, the green LED goes on. The second LED blinks yellow during data transfer.

  • Page 31: Possible Problems And Solutions

    Troubleshooting 7.2 Possible problems and solutions Problem Solution pH value Noisy or unstable If necessary, remove air bubble on the ƒ readings membrane of the pH flow cell. Drift solution temperatu- Allow all solutions to come to thermal ƒ re is changing equilibrium.

  • Page 32: Maintenance And Care

    Maintenance and care 8. Maintenance and care Proper maintenance of your devices will ensure successful analyses and repro ducible results. 8.1 Maintenance contract The following maintenance work on the device may only be performed by the manufacturer or a company authorized by the manufacturer and is covered by a separate maintenance contract: Opening the module.

  • Page 33: Cleaning Ph Flow Cell

    Maintenance and care Removal of salt deposits: Immerse the electrode in 0.1 M HCl for 5 minu- tes followed by 0.1 M NaOH for 5 minutes and then 0.1 M HCl for 5 minu- tes. Rinse the electrode with deionized water to remove all traces of HCl. 8.3.2 Cleaning pH flow cell If the pH flow cell is separated from the pH electrode, all chemicals suitable for PEEK surfaces can be used for manual cleaning.

  • Page 34: Transportation And Storage

    Transportation and storage 9. Transportation and storage Carefully prepare the device for transport or storage. If you want to return your device to KNAUER for repairs, enclose the Service request form which can be downloaded from our website. 9.1 Preparing wetted components pH flow cell and Flush the pH flow cell and pH electrode according to chapter 8.3.

  • Page 35: Storing The Device

    9.3 Storing the device 9.3.1 Storage of the pH monitor The functionality of the device remains warranted, if you select a storage loca tion according to the following ambient conditions: Temperature range 4 – 40 °C; 39.2 – 104 °F Air humidity Below 90%, non-condensing Air pressure 84 – 106 kPa;...

  • Page 36: Disposal

    10.1 AVV marking in Germany According to the German „Abfallverzeichnisverordnung“ (AVV) (January, 2001), old devices manufactured by KNAUER are marked as waste electri- cal and elec tronic equipment: 160214. 10.2 WEEE registration KNAUER as a company is registered by the WEEE number DE 34642789...

  • Page 37: Technical Data

    Technical data 11. Technical data Main features Device type pH monitor pH measurement range pH 0 - 14 pH accuracy pH 2 - 12: ±0.2 pH (15 - 25 °C), ±0.5 pH (4 - 40 °C) pH drift Maximum 0.02 pH/h at pH 4 Maximum data rate 5 Hz (LAN, RS-232) Outputs...

  • Page 38: Chemical Compatibility Of Wetted Materials

    The following list contains information about the chemical compatibility of all wetted materials which are used in devices made by KNAUER. The data bases on a literature research on the manufacturer specifications of the materials. The wetted materials of this device are listed in the chapter “Technical data”.
  • Page 39 Chemical compatibility of wetted materials Polyimide (Vespel®) This material is wear-resistant and permanent resilient thermically (up to 200 °C) as well as mechanically. It is chemically broadly inert (pH range 1-10) and is especially resistant against acidic to neutral and organic solvents, but vulnerable to pH strong chemical or oxidizing environments: It is incompatible with concentrated mineral acids (such as sulfuric acid), glacial acetic acid, DMSO and THF.

  • Page 40: Non-Metals

    Chemical compatibility of wetted materials Polychlortrifluorethylene (PCTFE, Kel-F®) The semi-crystalline thermoplastic material is plasticizer-free and dimen- sionally stable, even in a wide temperature range (−240 °C to +205 °C). It is moderately resistent against ether, halogenated solvents and toluene. Halogenated solvents over +60 °C and chlorine gas should not be used. Fluorinated rubber (FKM) The elastomer consisting of fluorinated hydrocarbon stands out due to a high resistance against mineral oils, synthetic hydraulic fluids, fuels,...

  • Page 41: Metals

    Chemical compatibility of wetted materials to corrosion and wear. The material is characterized by a high hardness and a high thermal conductivity. Mineral wool This insulating material consists of glass or stone wool fibres and isolates in high oxidizing conditions and at high temperatures. Mineral wool is valid as commonly inert against organic solvents and acids.

  • Page 42: Repeat Orders

    13. Repeat orders This list for reorders is valid for the time the document has been publis- hed. Deviations afterwards are possible. For reorders of spare parts use the enclosed packing list. Contact the technical support in case there are any questions on spare parts or accessories.
  • Page 43 Appendix: Installation Qualification (IQ)
  • Page 44 Installation Qualification (IQ) for a Device Customer approval Prior to installation at the customer site, the customer has reviewed the OQ document and agrees with the design and scope Company name: Name Function Reviewed & approved Date Signature VIQ-Installation-Qualification-Device Version 3.0 Revision: Page 1/7...
  • Page 45 KNAUER Wissenschaftliche Geräte GmbH. Scope The customer can request the Installation Qualification. In case of a request, the technical support of KNAUER or a provider authorized by KNAUER performs this functionality test during the installation. The IQ is a standardized document and includes the following: ƒ...
  • Page 46 Installation Qualification (IQ) for a Device Installation Qualification Tests Test Description Specification Passed Failed Comment /LOR No. Identify the device. The name on the device matches the name on the delivery order. Check the device for No transport damage is transport damage.
  • Page 47 Installation Qualification (IQ) for a Device List of rectifications (LOR) Type of Description of Persons LOR No. Test No. Action plan Due date Date/signature deviation* the deviation responsible * Type of deviation: A = acceptable (e.g. not a GMP-critical deviation) N = not acceptable Continuation of qualification activities into the next qualification phase is only possible when deviation is rectified.
  • Page 48 Installation Qualification (IQ) for a Device List of changes to the document Revision no. Description of change Additional information Date/signature VIQ-Installation-Qualification-Device Version 3.0 Revision: Page 5/7...
  • Page 49 Installation Qualification (IQ) for a Device 10. Certificate and appoval A KNAUER employee or an employee authorized by KNAUER has checked the device and performed all tests described in the IQ. The IQ form has to be signed by an authorized person. The scope of the IQ meets the customer‘s requirements.
  • Page 50 Installation Qualification (IQ) for a Device Appendix: List of supporting documents Test no. Description VIQ-Installation-Qualification-Device Version 3.0 Revision: Page 7/7...
  • Page 51 Latest KNAUER instructions online: www.knauer.net/library KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2023...

Table of Contents