Knauer K-7400S User Manual

Knauer K-7400S User Manual

Semi-micro freezing point osmometer
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K-7400S Semi-Micro Osmometer
Freezing Point Osmometer
User Manual

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Kiptoo Emmanuel from Kenya
March 29, 2025

We recently procured Semi-Micro Osmometer Product No: E0060XB. Does it have any compatibility with CFR 121 Compliant Software? If yes, how can we get it?

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Mr. Anderson
March 29, 2025

No, the Knauer K-7400S Semi-Micro Osmometer does not currently have compatibility with CFR 21 Part 11 compliant software, but development is in progress.

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Summary of Contents for Knauer K-7400S

  • Page 1 K-7400S Semi-Micro Osmometer Freezing Point Osmometer User Manual...
  • Page 2 Release date: 2017/07/12 Translation of the original edition The information in this document is subject to change without prior notice. For latest version of the manual, check our website: http://www.knauer.net/knowledge/user-manu- als.html Copyright: © KNAUER Wissenschaftliche Geräte GmbH 2017 All rights reserved.
  • Page 3: Table Of Contents

    Sample list ..............K-7400S user manual V3700...
  • Page 4 KNAUER Software License Agreement ........
  • Page 5: Product Information

    With this button, every running measurement can be interrupted. Activates the stirring wire. During RUN and CALIBRATE, the button is without any function. Arrows Cursor navigation buttons for parameter or value selec- tion K-7400S user manual V3700...
  • Page 6: Rear View

    Ventilator Power switch Power connector Fig. 2 Rear view Scope of delivery Note: Only use original parts and accessories made by KNAUER or a com- pany authorized by KNAUER.  Freezing Point Osmometer K-7400S  Power cable  User manual (German/English) ...
  • Page 7: Signal Words

    Decontamination Devices without a completed Decontamination Report will not be report repaired. If you would like to return a device to KNAUER, make sure to enclose a completed Decontamination Report with the device: http:// www.knauer.net/en/downloads/service.html...
  • Page 8: Symbols And Signs

    At least 10 cm, if there are devices set up on both sides.  At least 15 cm to the cooler fan on the rear. Ambient temperature The device should not be exposed to major temperature fluctuations during measurement. K-7400S user manual V3700...
  • Page 9: Power Supply

    Electronic hazard when using an identically constructed power adapter from another manufacturer.  Only use original parts and accessories made by KNAUER or a company authorized by KNAUER. Note: The nominal capacity of the connected devices must be maximum 50 % of the power supply to account for larger inrush currents when switching on the modules.
  • Page 10: Start-Up

    2. Switch the device on by using the "ON/OFF" switch. Next Steps Bring the device into operation. To put the device out of operation, switch off the device and detach the power plug from the power supply. K-7400S user manual V3700...
  • Page 11: Operation

    Measuring head Fig. 4 Sample tube Note: Any change of the measuring head causes the need for a precalibra- tion of the device to adapt the device to the measuring head (see “Pre-cal- ibration” on page 12). K-7400S user manual V3700...
  • Page 12: Stand-By Position Of Measuring Head

    -8 °C without freezing. The freezing is initiated by an automatically start of the stirring wire at a set temperature. The temperature reaches the freezing point, 0 °C in case of pure water (Fig. 6). K-7400S user manual V3700...
  • Page 13 After finishing the measurement, the sample tube is warmed up to 20 °C (stand-by temperature of the device). The measuring head can remain there before and after measurement as long as desired. K-7400S user manual V3700...
  • Page 14: Switch-On

    . In this way, you can e. g. choose between printer and PC for data out- put (screen 2) or display the measurement data in mOsmol or °C (screen Fig. 8 Menu path Screen Explanation Main menu 2 Data Output Controls the connection of the Osmometer with a printer or PC. K-7400S user manual V3700...
  • Page 15: Preparing A Measurement

    Note: If you want to print out the measurement results, you may connect a printer via RS-232 connector. The result of each measurement will be printed automatically in the follow- ing format: Measured 0400 mOsmol 23.03.15 08:24:35 K-7400S user manual V3700...
  • Page 16: Measurement Preparation Process

    00:56 3. After finishing the calibration, the screen displays the message "CALIBRATE-T sample? >successful<". To confirm the calibration press the <START> button once. Afterwards the initial CALIBRATE-T menu is displayed and previous calibration values are deleted. K-7400S user manual V3700...
  • Page 17: Measurement Calibration

    Performing the calibration Prerequisites Prepare a measurement with water (HPLC quality, see "Measurement preparation process" on page 12). Process 1. Activate the CALIBRATE screen and set the first calibration field to "0000". K-7400S user manual V3700...
  • Page 18: Measuring Sample Solutions

    If the crystallisation occurred properly, it displays the corresponding osmolality on the display: >>> 1234 mOsmol <<<  <next sample> The printer output is given in the following format: Measured 1234 mOsmol 19.03.15 16:05:35 K-7400S user manual V3700...
  • Page 19: Important Operating Notes

    Important operating notes 1. The device is only suitable for aqueous solutions. 2. Only use sample tubes which were obtained from KNAUER or recom- mended by KNAUER. Sample tubes should not be reused. 3. To ensure a fixed sample volume, always introduce the solutions with a clean and dry pipette tip into the sample tube.
  • Page 20: Functionality Tests

    Functionality tests Installation Qualifica- The customer may request the Installation Qualification, which is free of charge. In case of a request, the Technical Support of KNAUER or from a tion (IQ) provider authorized by KNAUER performs this functionality test during the installation.
  • Page 21: Self-Freezing Of The Sample

    Only use sample tubes which causing premature freezing. were obtained from KNAUER or recommended by KNAUER. Solutions saturated with air or Degas the sample solution by gases are sometimes difficult to ultrasound.
  • Page 22: Non-Freezing Of The Sample

    4. If there is no AutoPlay menu, select Explore from the right-click menu, go to EuroOsmo 7400/Disk1 and double-click on <Setup.exe>. Hardware installation The device has to be connected with a defined port of the computer (e.g. COM 1) prior to starting the program. K-7400S user manual V3700...
  • Page 23: Main Screen

    Displays a preview of the actual report. Starts the search function. Pastes the data of a selected field into following lines of the chosen column. After each bar code scan, the cursor switches to the next sample line. K-7400S user manual V3700...
  • Page 24: Run Settings

    Fig. 12 New sample list Double clicking on <No.> of an already measured sample dis- plays the corresponding temperature sequence. The path of the already stored sample list is shown on top of the graph. K-7400S user manual V3700...
  • Page 25: Setup

    The entered value defines the scale of the monitor by its final value in minutes. The temperature pat- tern is shown continuously for the defined period. Measurement The entered value defines the scale of the mea- view surement by its final value in minutes. K-7400S user manual V3700...
  • Page 26 If any value is out of the allowed range a corresponding mes- sage will be displayed. The setting of the osmom- eter remains unchanged. Fig. 15 Error message in case of not valid parameters Fig. 16 Report design area K-7400S user manual V3700...
  • Page 27: Report & Graph

    As wildcard characters can be used ? for single characters and * for undefined areas before and/or after to the defined search characters. If necessary the search results can be reduced by advanced options. These are the measurement values and/or the date of the measurement. K-7400S user manual V3700...
  • Page 28: Maintenance And Care

    <MEASUREMENT TIMEOUT> on the display.  3. Press quickly twice the button to read out the cooling cavity value on the COOL menu (see page 10). 4. The value must be in the range of -13 to -16 °C. K-7400S user manual V3700...
  • Page 29: Reference Thermometer

    Two-Point Calibration (0 and one free selectable osmolality) optional: Three-Point Calibration (0 and 2 free selectable osmolalities) Measuring procedure Completely automatic after sample insertion Communication Port RS-232 Control Keypad (LED screen, 2 rows with 24 caracters), software K-7400S user manual V3700...
  • Page 30: General

    Technical Support in case there are any questions on spare parts or acces- sories. Further information Further information on spare parts and accessories can be found online: www.knauer.net Name Order no. Freezing point osmometer K-7400S A0006AC Power cable 230V M1479 RS-232 Connection cable M1702 Plain printer...
  • Page 31: Warranty Conditions

    2001), old devices manufactured by KNAUER are marked as waste electri- cal and electronic equipment: 160214. WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German "Elektroaltgeräteregister" (EAR). The number belongs to cate- gory 8 and 9, which, among others, comprise laboratory equipment.
  • Page 32 Licensee may terminate the license at any time by destroying the Software (including the related documentation) together with all copies or modifica- tions in any form. KNAUER will have the right to terminate the Licensee's license immediately if the Licensee fails to comply with all copies or modifi- cations in any form.
  • Page 33 Licensee may also have other rights which vary from country to country. 7. Limitation of Liability. IN NO EVENT WILL KNAUER BE LIABLE TO LICENSEE FOR ANY SPECIAL DAMAGES, INCLUDING ANY LOST PROFITS, LOST SAVINGS, OR OTHER INCIDENTAL OR CONSEQUENTIAL DAMAGES - EVEN IF KNAUER HAS BEEN ADVISED OF THE POSSIBILTY OF SUCH DAMAGES, OR FOR ANY CLAIM BY ANY OTHER PARTY.
  • Page 34: Index

    Software Hardware installation Installation Main screen solvent self-ignition point space requirements spare parts stirring wire symbols K-7400S user manual V3700...
  • Page 35 Latest KNAUER manuals online: www.knauer.net/knowledge KNAUER Phone: +49 30 809727-0 Wissenschaftliche Geräte GmbH Fax: +49 30 8015010 Hegauer Weg 38 E-Mail: info@knauer.net 14163 Berlin Internet: www.knauer.net © KNAUER 2017...

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