Nippon Genetics FastGene qFYR User Manual
  1. Manuals
  2. Brands
  3. Nippon Genetics Manuals
  4. Laboratory Equipment
  5. FastGene qFYR
  6. User manual

Nippon Genetics FastGene qFYR User Manual

Real-time pcr system
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Real-Time PCR System
FastGene
qFYR Real-Time PCR System
®
User Manual
Version 1.0

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the FastGene qFYR and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Nippon Genetics FastGene qFYR

Summary of Contents for Nippon Genetics FastGene qFYR

  • Page 1 Real-Time PCR System FastGene qFYR Real-Time PCR System ® User Manual Version 1.0...
  • Page 2 ® Copyright & Disclaimer, NIPPON Genetics EUROPE GmbH The copyright of this manual is owned by NIPPON Genetics EUROPE GmbH. No individual or organization shall, in any form, copy, edit, publish, or translate the contents of this manual (including but not limited to words, trademarks, logos, keystroke icons, lists, images, data, etc.) into other languages without the authorization or written permission of the company.
  • Page 3 ® Introduction Welcome to the FastGene ® qFYR Real-Time PCR System. Please read this instruction carefully so that you can use this instrument correctly. After careful reading, please keep it for future inquiry when necessary. Product information Description: Real-Time PCR System Product model: FastGene qFYR Realtime PCR System...
  • Page 4 ® Enterprise information Statement of intended use: This product is used to run real-time fluorescence PCR experiments (Real-time PCR) and to analyze the experimental data. The instrument is operated in a laboratory with the corresponding reagents, to carry out rapid and accurate quantitative and qualitative detection of target nucleic acids sequences from human and animal samples (such as blood, oral swabs, nasopharyngeal swabs, body fluids, etc.) or other analytes, or to conduct melting curves, genotype analysis, etc.

  • Page 6: Table Of Contents

    Table of Contents Table of Contents Chapter 1 Security and considerations Security Symbols and Markings 1.2 General Instrument Safety and Precautions 1.3 Personal Safety and Precautions 1.4 Electrical Safety and Precautions 1.5 Environmental Safety and Precautions 1.6 Biosafety and Precautions 1.7 Electromagnetic Compatibility (EMC) Standards Chapter 2 Product Overview...
  • Page 7 3.4.1 Instrument Working Environment Requirements 3.4.2 Computer Configuration Requirements 3.5 Equipment Installation 3.5.1 Equipment Appearance 3.5.2 Release Instrument Transport Lock 3.5.3 First Installation and Run Protocol 3.6 Instrument Software Installation 3.6.1 FastGene® qFYR Analysis Studio Software Installation 3.7 Pre-preparation for boot 3.7.1 Instrument Self-inspection 3.7.2...
  • Page 8 Chapter 5 Operation of Different Experiment Types 5.1 Absolute Quantification (Standard Curve) Experiment 5.1.1 Setup of a Standard curve 5.2 Experiment of Melting Curve 5.2.1 Setup of Melt Curve Analysis 5.3 Relative Quantification Experiment Cq 5.3.1 Setup of comparative Cq ( Cq) Analysis 5.4 Genotyping Experiments...
  • Page 9 6.2.13 Genotyping Maps 6.2.14 Raw Melt Curves of the High-Resolution Melt analysis 6.2.15 Aligned Melt Curves of the High-Resolution Melt analysis 6.2.16 Difference plot of the High-Resolution Melt analysis 6.2.17 Presence/Absence Plot 6.2.18 QC Summary 6.3 Data Export 6.3.1 Toolbars in Export Tab 6.3.2 Data Export 6.3.3...

  • Page 10: Chapter 1 Security And Considerations

    ® Safety markings used on instruments Symbol Heading Description This symbol is used to indicate that non- compliance with instructions or procedures may lead to physical injury or even Caution death or could cause damage to the instrument. Consult the Operator's Manual.

  • Page 11: Personal Safety And Precautions

    ® It is forbidden to carry or move the instrument during the operation of the instrument. The openings on this instrument are ventilated. In order to avoid excessive temperature, do not block or cover these vents during the operation of the instrument, or use dust shields and any other substances to cover the surface of the instrument.

  • Page 12: Environmental Safety And Precautions

    ® The shell of this instrument should be properly grounded through the power ground wire. Any damage to the internal or external grounding path of the instrument may be dangerous. Note: If leakage is found, please cut off the power immediately and stop using the instrument. Note: Please cut off the power supply before moving the instrument.

  • Page 13: Biosafety And Precautions

    ® Biosafety and Precautions Biological hazard The sample object of this instrument is nucleic acid. In practice, please regard it as a biological sample with potential biological hazard. When handling and operating samples, generally applicable safety precautions shall be taken and appropriate protective goggles, clothing and gloves shall be worn.
  • Page 14 ® Note: It is the responsibility of the user to ensure the EMC environment of the instrument so that the instrument can work properly. FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 15: Chapter 2 Product Overview

    ® Chapter 2 Product Overview Scope of application 2.1.1 Intended Use FastGene qFYR Real-Time PCR Systems are used to run real-time fluorescence PCR ® experiments (Real-time PCR) and to analyze the experimental data. The instruments are operated in a laboratory, in conjunction with the corresponding reagents, for rapid and accurate quantitative and qualitative detection of target nucleic acids from human samples (e.g., blood, oral swabs, nasopharyngeal swabs, body fluids, etc.) or other analytes in the patient's body, or for melting curves, genotype analysis.

  • Page 16: Basic Principles

    ® Basic Principles PCR principle is similar to the natural replication process of DNA. Its specificity depends on oligonucleotide primers complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension. Repeated cyclic denaturation- annealing-extending processes can amplify the target gene by millions of times in 1~2 hours, for a high detection sensitivity.

  • Page 17: Product Structure

    ® Product Structure Diagram of the Structure of the Machine 1. Sample module 2. Sample draw 3. Inlet 4. Transport lock 5. Power indicator 6. Status indicator 7. In/Out button 8. Vent 9. Power switch 10. power cord socket 11. USB interface 12.
  • Page 18 ® Schematic diagram of temperature control module Schematic diagram of optical path module Schematic illustration of optical scanning patterns FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 19: Instrument Parameters And Characteristics

    ® Instrument Parameters and Characteristics 2.7.1 Physical Specifications Size: 350 mm(L) × 520 mm (W) × 370 mm (H). Instrument specifications Weight 25 kg Size: 710 mm(L) × 540 mm (W) × 580 mm (H). Packing specifications Weight 36 kg power supply voltage AC 110-230 V Frequency 50 Hz.

  • Page 20: Instrument Technical Parameters

    ® 2.7.2 Instrument Technical Parameters Thermal parameters Peltier semiconductor Heat cycle Temperature control module Temperature control mode Maximum ramp rate ± 0.2 °C Temperature accuracy ± 0.2 Temperature uniformity Optical parameters Single color LED Excitation source High sensitivity MPPC（silicon photomultiplier tube） Detection device 4 channels (2x FAM) Fluorescent channel number...

  • Page 21: Software Features

    ® 2.7.3 Software Features Software interface: Wizard interface, intuitive sample plate layout and program settings, easy to use operation editing. Software functions: absolute quantitation, relative quantitation, genotyping, HRM-analysis, and other analytical functions to meet the requirements of a variety of experiments. Language Support: English.

  • Page 22: Chapter 3 Installation And Transportation Of Instruments

    Please check the content you received if parts are missing and damaged after unpacking. If the product is damaged during transportation, please do not use it, and contact NIPPON Genetics EUROPE or the distributor! FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide...

  • Page 23: Packing List

    ® Packing List NAME Unit Real-Time PCR System FastGene qFYR ® Instrument power cable Instrument data cable Fuse hexagon spanner Packing List USB Stick (including software and electronic user manual) Installation Requirements 3.4.1 Instrument Working Environment Requirements Location: The main unit must be placed on a stable, horizontal working table, avoiding direct sunlight, not near heating equipment.
  • Page 24 NIPPON Genetics EUROPE is not responsible for any damage caused by an internet connection. It is not recommended to install other software in the control computer. There will be a potential risk of conflict with the instrument software module and may affect the reliability of the results.

  • Page 25: Equipment Installation

    ® Equipment Installation The following contents are convenient for users to quickly complete the installation of the FastGene qFYR Real-Time PCR System. Please be sure to operate according to the following ® contents. If you have any questions during the installation operation, please contact our support. 3.5.1 Equipment Appearance FastGene ®...
  • Page 26 ® Status Lamp There are power status light “POW” and system status light “STU” on the equipment´s top part. The meaning of status lights is shown in the following: Name Status Means Blue light Ready Green light slowly flashing Self-checking Green light quickly flashing Working Green light on...

  • Page 27: Release Instrument Transport Lock

    ® Data communication The interface of the device's back panel is the USB, which is used for communication between the computer and the device. Air inlet The lower gap of the front panel of the equipment is a set of air intakes. Blocking must be prevented when running.

  • Page 28: First Installation And Run Protocol

    ® 3.5.3 First Installation and Run Protocol Insert the USB data cable and the power cable (as shown in figure A below) and connect data cable to the computer. After turning on the power supply, press the power switch at the back end of the instrument to turn on the instrument, and wait until the power indicator light (POW) is constantly on, and the system begins self-checking process.

  • Page 29: Instrument Software Installation

    ® Instrument Software Installation 3.6.1 FastGene qFYR Analysis Studio Software Installation ® You need to install the FastGene qFYR Analysis Studio software in your computer before using ® the FastGene qFYR Real-Time PCR System. You can find the installation software in the ®...
  • Page 30 ® 5. Click install to complete the program installation 6. You need to pre-install drivers in the system before using FastGene ® qFYR Real-Time PCR System and the FastGene qFYR Analysis Studio software. After installation of the ® FastGene ® qFYR Analysis Studio software, please click on the install button in the window below: 7.
  • Page 31 ® 8. The installation will be confirmed: 9. Please close the window of the driver installer and press Next on the window below: 10. The installation is complete by clicking Finish FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 32: Pre-Preparation For Boot

    ® Pre-preparation for boot 3.7.1 Instrument Self-inspection This instrument has the function of self-inspection. Before use, the user should run the instrument´s self-inspection to ensure that the instrument can work normally during the experiment. 1. Turn the power switch on at the back of the instrument and turn on the instrument. 2.

  • Page 33: Experimental Preparation

    ® 3.7.3 Experimental Preparation Good laboratory practice for PCR and RT-PCR When preparing PCR or RT-PCR amplification samples Use only clean personal protection equipment and lab coat  Whenever you suspect that the gloves are contaminated, it is recommended to change ...

  • Page 34: Chapter 4 Operation To Run The Experiment

    ® Chapter 4 Operation to Run the Experiment This section will describe in detail how to set up and run experiments using FastGene qFYR ® Analysis Studio software. Software Start 1. Double-click the desktop software icon to launch the software 2.
  • Page 35 ® The next window will let you add, edit (“Update”), or delete users. 5. Startup interface will be shown: FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 36: Create New Experiment

    ® Create New Experiment The Startup interface is used to create a new experiment, run, or open previously saved templates to create a new experiment. 4.2.1 New Experiment or Loading existing files Click the "Experiment Setup" button at the bottom right of the startup interface or select "New Experiment"...

  • Page 37: Experiment Properties

    ® Experiment Properties The "Experiment Properties" interface is used to define the relevant properties of the experiment according to the actual experiment. This includes the name and type of the experiment, as well as the selection of the reaction tube and PCR reagent. 4.3.1 Name The default setting for the experiment name is the current time and date, which can be changed as needed.

  • Page 38: Experiment Type

    ® 4.3.3 Experiment type Select the experiment type according to the actual purpose of the experiment. The software will associate the subsequent experiment settings according to the selected experiment type. For the experiment type, please refer to the following table: Type of experiment Explanation Absolute quantification based on a standard curve is a real-time...

  • Page 39: Chemistry

    ® 4.3.4 Chemistry The instrument is compatible with most of the fluorescent quantitative PCR reagent consumables in the market. You can choose the probe method (TaqMan reagent) or the dye method (SYBR ™ ™ Green reagent) to carry out subsequent experiments according to the specific experimental design.

  • Page 40: Experiment Method

    ® Experiment Method The "Method" interface is used to set the specific parameters of a quantitative PCR, including reaction volume, stage, step, temperature, time, and cycle number, etc. The above parameters can be carried out according to specific experimental needs and corresponding reaction reagent requirements.
  • Page 41 ® Light up the camera icon to select the data collection point The camera is turned on by default to collect data after the extension is completed If you need to add a reaction step in a certain stage of PCR, hover the mouse to the bottom left or right step area and click "+"...

  • Page 42: Plate Setup

    ® Plate Setup The “Plate Setup” interface is used to set the properties of the reaction wells, including assigning samples, test targets, reaction well tasks and other properties to each reaction well. This will make the identification of the sample and the subsequent analysis possible. Note: The plate setup can be performed before, during and after the experiment.
  • Page 43 ® 4. Assign the sample (nucleic acid source) and the target item (gene of interest) to the selected well position and click the text box of each field to name the sample and target gene for actual test. 5. When you enter a new sample or target item in the “Setup” window, the software will automatically fill the Reporter with default values (FAM/SYBR) and quenching groups (NFQ- MGB) and assign tasks (unknown).

  • Page 44: Quick Setup

    ® 4.5.2 Quick Setup "Quick Setup" is a function module for quick and easy setup of samples and targets. After selecting the well position to be set, you can click the sample text box and target text box to manually enter the name. If settings have been made in the “Setup”, you can select the options defined in the “Setup”...

  • Page 45: Run An Experiment

    ® Run an Experiment 4.6.1 Insert a Plate or Reaction tubes Press the access button on the top of the instrument. The instrument´s heating block will be ejected. Load the reaction tube or plate onto the 96-well block. Note: When the drawer is closed, the heating lid will apply the appropriate pressure to securely place the tube band in the module.
  • Page 46 ® When the temperature of the heating lid reaches 100 ° C, the real-time heating lid´s and reaction plate´s temperature, and the cycle number of the reaction will be displayed in the upper left corner of the running interface. Note: In the initial reaction cycles, the original data map is most of the background or baseline. When the amplification reaction enters the exponential growth phase, the curve will rise.

  • Page 47: Save Experiment

    ® Save Experiment Click the "File" option in the menu bar, choose “Save” or “Save as” to save the original experimental data. At each step of the experiment process, you can choose to save the experiment in the upper right corner of the interface. The extension of the experiment file saved before running is .qpt (experiment running template).

  • Page 48: Chapter 5 Operation Of Different Experiment Types

    ® Chapter 5 Operation of Different Experiment Types FastGene qFYR Real-Time PCR System experiments are divided into seven steps: ® Create an experiment Set Experimental Properties Set the operating conditions Set the samples and targets Start the experiment View and analyze the results Export the experimental results Please see Chapter 4 for how to create, run, analyze, and review an experiment.

  • Page 49: Setup Of A Standard Curve

    ® Type of response: Many samples can be analyzed in standard curve experiments, but each sample needs its own standard curve. The following table lists the types of sample settings involved in the standard curve experiment: Type Setting Example Description Standard contains a known number of target samples Unknown...
  • Page 50 ® 5. Right-click in any area of the plate view and select “Define and Set Up standards”. 6. Select the target, which should be used for generation of the standard curve. Note: Singleplex means that there is only one gene to be tested in each reaction well, and Multiplex means that there are two or more genes to be tested in each well.
  • Page 51 ® 8. Select and arrange standard wells. The software can automatically select the reaction well by columns or rows or the • operator can select their own reaction well according to the need and arrange the well position of the standard product. After the standard well position distribution is set, the software will arrange the •...

  • Page 52: Experiment Of Melting Curve

    ® Experiment of Melting Curve 5.2.1 Setup of Melt Curve Analysis 1. Select experiment type “Melt Curve”. 2. Choose the used Plastic type and Chemistry. 3. Add name and optional information. 4. After the Method setting is completed, enter the Plate section, or click in the lower right corner of the page to enter the well plate setting interface.

  • Page 53: Relative Quantification Experiment Cq

    ® Relative Quantification Experiment（△△Cq） Relative quantification is suitable for most gene expression studies and can analyze the up- or down-regulation of target gene expression levels in calibrated (normal) samples and one or more experimental samples. Using this technology does not require accurate determination of copy number but focuses on multiple changes compared with calibration samples.

  • Page 54: Genotyping Experiments

    ® Genotyping Experiments In the study of gene variation, in order to obtain a large number of characteristic markers, a large number of individuals must be genotyped. The reasons for the differences in individual genomes at different levels include single nucleotide polymorphisms (SNP) and DNA covalent modification. more than 90% of the gene sequence variants that cause individual differences belong to single nucleotide polymorphisms (SNP).

  • Page 55: Set Up Of A High-Resolution Melt Analysis (Hrm)

    ® After the Method setting is completed, enter the Plate section, or click in the lower right corner of the page to enter the well plate setting interface. Note: The plate setup can be set before, during, or after the reaction operation. 5.4.2 Set Up of a High-Resolution Melt Analysis (HRM) Select experiment type “High-Resolution Melt”.

  • Page 56: Presence / Absence Experiments

    ® Presence / Absence Experiments Presence / Absence experiments are endpoint experiments that can be performed to determine whether a target is present or absent in a sample. This type of experiment can be used for the detection of pathogens. Presence / Absence experiments can only be performed using a probe-based assay and data is collected at the end.

  • Page 57: Chapter 6 Result Analysis And Data Export

    ® Chapter 6 Result Analysis and Data Export This chapter explains the data overview, analysis, and export. Result Tab 6.1.1 Buttons in Toolbar Button Name Function zoom in Zoom in for plate overview and curves zoom out Zoom out for plate overview and curves Return return to last step Plate view...

  • Page 58: Other Toolbars

    ® 6.1.2 Other Toolbars Toolbar Name Function Click to analysis result after changing plate or plot Analyze settings Action Click to select sample or target Save Save current results as .qps file View View current results by plate or list format Group by Click to show results by different groups Expand/Shrink...

  • Page 59: Result Overview

    ® Result Overview FastGene qFYR Analysis Studio will analyze experiment results by default settings after an ® experiment is finished, and show amplification plot in the Result tab. Attention: After omitting wells or changing any settings, please click to obtain new results. 6.2.1 Amplification plot Enter the Amplification plot tab to view the amplification curve.

  • Page 60: Plot Settings

    ® 6.2.2 Plot Settings Select to enter the Plotting Settings and to adjust plot type, graph type, threshold etc. Use the drop-down menu to choose the Plot Type. The default setting is “Rn vs cycle”, but “△Rn vs cycle”, “Cq vs well” are available as well. Plot Type Description Purpose...

  • Page 61: Baseline Settings

    ® 6.2.4 Baseline Settings The software automatically sets the baseline in the first few cycles of the reaction, usually withing 3 to 15 cycles. If you need to set the baseline manually, remove the automatic threshold check, you can drag and drop the baseline (green triangle) directly on the curve and change it within the to 15 cycle.

  • Page 62: Results As Reaction Board

    ® 6.2.6 Results as Reaction Board To view the results in plate format, click In the plate view, the information of each well (incl. target, task, dye, sample) will be showed while the mouse is moved to and paused on each well. E.g., information of well B4 as in figure above. If a different well is selected, the amplification plot will be shown accordingly.

  • Page 63: Results As List

    ® 6.2.7 Results as List To display the results as list, click All the experiment information is listed, including well, sample, target, task, Cq, Cq mean, Cq SD etc. The list can be grouped by the following: Please note that sections 6.3.1 to 6.2.7 can be applied to all subsequent plot types. FastGene ®...

  • Page 64: Raw Amplification Plot And Raw Melt Peak Plot

    ® 6.2.8 Raw Amplification Plot and Raw Melt Peak Plot Raw plots present the real time fluorescence signal change. The cycle number is shown on the X- Axis while the fluorescence signal value is plotted on the Y-Axis. By entering the corresponding tabs multicomponent plots for the amplification stage or for the melt curve step will be shown.

  • Page 65: Standard Curve

    ® 6.2.9 Standard Curve Standard curves can be used to verify the amplification efficiency of comparative Cq experiments or to perform an absolute quantitative PCR experiment. Click Standard Curve View standard Note: The amplification efficiency (Eff%), slope, Y-Intercept, R2 and Error will be shown below the graph.

  • Page 66: Melt Curve

    ® 6.2.10 Melt Curve The melt curve shows the reduction in fluorescence caused by the denaturation of the DNA double helix during the final slow heating step (melt curve analysis). Enter Melt curve plot in the menu. View derivative of the melt curve fluorescence plot Note: Melt curve plot is applied to check the specificity of PCR reaction and products: Two or more peaks are indicators of an unspecific amplification or of multiple amplicons.

  • Page 67: Gene Expression Map

    ® 6.2.11 Gene Expression Map Gene expression maps can be used to view the results of relative quantitation calculations and the gene expression profile. Click Gene Expression All sample information is shown in the selected table Check the gene expression map: Note: There are two plot types available: RQ vs Target (relative quantitation (RQ) values are grouped by target), and RQ vs Sample (relative quantitation (RQ) values are grouped by sample).

  • Page 68: Analysis Settings

    ® 6.2.12 Analysis Settings General Analysis settings can be changed by entering the Analysis settings in the menu toolbar: Enter the tab Relative Quantification Settings to edit settings used for relative quantification of data, such as References, Efficiency of used primers, Outlier Rejection and RQ Min/Max calculations.

  • Page 69: Genotyping Maps

    ® 6.2.13 Genotyping Maps The allelic discrimination plot clusters the normalized reporter fluorescence (Rn) of the allele- specific probes used in the SNP assay for the three possible genotypes (Allele 1 homozygous, Allele 2 homozygous, and Allele 1/2 heterozygous) as well as for negative controls (undetermined) Click the allele identification map.

  • Page 70: Raw Melt Curves Of The High-Resolution Melt Analysis

    ® 6.2.14 Raw Melt Curves of the High-Resolution Melt analysis The melt profile of a high-Resolution Melt analysis (HRM) reflects the mix of amplicons present in the sample. Variations in sample characteristics like GC content, length, sequence, and heterozygosity are defining the melt curve characteristics of each amplicon. Click the Raw Melt curves tab.

  • Page 71: Aligned Melt Curves Of The High-Resolution Melt Analysis

    ® 6.2.15 Aligned Melt Curves of the High-Resolution Melt analysis During HRM two types of melt curves can be observed. On the one hand, melt curves that differ from each other in melting temperature (T ) of the amplicon, but are similar in curve shape. Usually, this kind of curve is generated by homozygous variant samples.

  • Page 72: Difference Plot Of The High-Resolution Melt Analysis

    ® 6.2.16 Difference plot of the High-Resolution Melt analysis The difference plot is a tool to easily visualize small differences in melt curves of HRM analysis. Curves displayed here are generated by the differences between samples and a single reference. Click the Difference Plot tab.
  • Page 73 ® sample to be divided into different variation groups, even if they have similar curve characteristics. Default settings can be changed by opening the analysis settings in the menu toolbar. In the tab “HRM Settings” uncheck “Automatically determine the number of variant groups” and enter the desired number.

  • Page 74: Presence/Absence Plot

    ® 6.2.17 Presence/Absence Plot The Presence/Absence plot display the fluorescence intensity measured in each well. Enter the Presence/Absence plot tab. Check the Presence/Absence results. There are 4 types of plot views that can be chosen in the Plot settings: “All calls” – all calls are displayed, “Presence”...

  • Page 75: Qc Summary

    ® Targets are called regarding their fluorescence intensity: • Call is present if fluorescence intensity is higher than the target threshold, Call is absent if fluorescence intensity is lower than the target threshold, and fluorescence intensity of the IPC is higher than the IPC threshold, Call is unconfirmed if fluorescence intensity is lower than the target threshold, and fluorescence intensity of the IPC is lower than the IPC threshold.
  • Page 76 ® Confirm by clicking and click after Flag setting is changed. FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 77: Data Export

    ® Data Export 6.3.1 Toolbars in Export Tab Toolbars Name Function Export Export data Save Save as .qps file 6.3.2 Data Export File name: The default file name is the name defined in “Plot properties” or could be changed to customized name. English characters are supported, but not special characters. Export content: Include sample setup, Amplification data, Multicomponent data, Results, Melt Curve Result.
  • Page 78 ® The export is shown in Excel as below. FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 79: Printed Report

    ® 6.3.3 Printed report Next to data export, a report of the complete data for an experiment, including graphs, can be saved, and printed. In the result section, select the wells that should be reported Access the main menu toolbar, enter the “File” drop down and click “Print Report” A pop-up menu will open to select the data and graphs to be reported Report can either be directly send to a connected printer by clicking previewed and saved by clicking...
  • Page 80 ® FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...

  • Page 81: Chapter 7 Instruments Maintenance

    ® Chapter 7 Instruments Maintenance This instrument does not need a lot of maintenance in case of routine use. If this instrument is used for a long time, it is necessary to clean and maintain the instrument regularly to ensure normal instrument operation.

  • Page 82: Cleaning The Instrument

    ® 7.1.1 Cleaning the Instrument Turn off the instrument and unplug the power cord. Use a damp soft cloth to clean the instrument shell, use a mild cleaner to clean if necessary. Note: Do not spray the cleaner directly on the instrument, which may cause the failure of the electronic instrument.

  • Page 83: Instrument Maintenance

    ® 7.2 Instrument maintenance 7.2.1 Keep Air-Circulation The instrument must be positioned with a possibility of free air circulation to ensure accuracy of the target temperature. Please check the placement area regularly to ensure a free air circulation, and no items that can affect the air flow. 7.2.2 Keep Stable Electricity Supply A stable power supply is required for the normal operation, please check it regularly to ensure that the supply voltage is consistent with the voltage requirement (±10% deviation is allowed).

  • Page 84: Fuse Replacement

    ® 7.2.5 Fuse Replacement When the circuit fails or is abnormal, current increase or rising of the current may damage important components. If the fuse is installed correctly, the fuse will break when the current rises to a certain height and heat, to protect the instrument or the user.

  • Page 85: Chapter 8 Troubleshooting

    ® Chapter 8 Troubleshooting a) Observation: Switch on the instrument, but no reaction, and power light off. Possible cause: Inconsistent connection with power cable Recommended action: Switch off the power on the rear of instrument, re-plug power cable. Inconsistent plug correct plug b) Observation: Shows “No Available Instrument”...
  • Page 86 ® c) Observation: There is no fluorescence signal appearing on “Multicomponent plot”, while the sound of scanning can be heard. Possible cause: The "sample" and "target item" columns on the "well-plate setup" interface are not filled in. The fluorescence signal is displayed before filled in both. If the user does not fill in "sample"...
  • Page 87 ® Observation: While scanning empty block, background signal of some wells is much higher than average. Possible cause: There are contamination in these wells. Recommended action: Clean the contamination, please refer to Section 5.3 Monthly maintenance. Observation: Reaction reagent been evaporated Possible cause: The tubes or seal on plate are not closed correctly.
  • Page 90 ® NIPPON Genetics EUROPE GmbH Production Permit Number: Product Registration Certificate No./ Product Technical Requirements No.: Production Enterprise: NIPPON Genetics EUROPE GmbH Address: Mariaweilerstraße 28-30 | 52349 | Düren |Germany Tel: +49 2421 554960 Web: www.nippongenetics.eu FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide...
  • Page 91 ® FastGene qFYR Real-Time PCR Systems Installation, Use, and Maintenance Guide ®...
  • Page 92 Referenznr. NIPPON Genetics EUROPE GmbH +49 2421 554960 info@nippongenetics.de +49 2421 55496 11 www.nippongenetics.eu...

Table of Contents