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8. Validation
intenance
It is recommended that the HPLC system be validated from time to time to maintain
the accuracy and reliability of analysis. Thin task of validation has been made easier
due to the unique and user-friendly feature of Shodex RI-504.
SPAN CHECK
S.SEQ
DISPLAY
TEMPERATURE
LAMP VOLTAGE
DRIFT
NOISE
SPAN
Do the Span Validation, follow the procedures below :
1) Preparation of standard sucrose solution :
Weigh 350 mg of sucrose and transfer it to a 100 mL volumetric flask.
Dissolve it with deionized, filtered, degassed water until the solution reaches the
mark on the flask.
Note
Press the
Enter
screen.
（Refer to the following for the details.）
Press the
S.Seq
The sequence is complete and the sign (
come up on the Monitor screen.
This indicates the difference between the set temperature
and the actual temperature in degree Celsius.
When the difference becomes
a message("GOOD") will appear in the Monitor
screen.
This indicates the voltage applied to the lamp. When the
Voltage falls 4.9V, a message("GOOD") will appear in
the screen.
This indicates a result of "S.SEQ" .
When the drift falls
in the screen.
This indicates a result of "S.SEQ" .
When the noise falls
D") will appear in the screen.
This indicates a result of "SPAN CHECK" .
Please check whether the display value is "487-
537" μ RIU.
Always use freshly made sucrose solution.
- 22 -
key to obtain the "SPAN VALIDATION"
key once to call the start-up sequence.
"+ or -" 1 degree,
within
, a message("GOOD") will appear
set value
, a message("GOO
set value
or"FAIL") will
RD
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