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6-4 Operation Procedures
Use the detector in accordance with the following procedures:
[ Automatic Start up ]
1) Set parameters on the operating parameter-setting screen.
2) Set parameters on the start-up sequence parameter-setting screen.
3) Start pumping the eluent at a flow rate of 1 mL/min.
If pumping can be done only at a flow rate lower than 1 mL/min,
a longer purge cycle needs to be set.
4) Press the
S.Seq
The time elapsed from the sequence start and the total sequence time are displayed on
the screen.
8) If the drift or noise meets the target value within the defined period (Measuring Time),
a message("GOOD") will appear in the Monitor screen.
9) When the
S.Seq
When the
ESC
start-up sequence finished.
Though the RD sign does not come up, the detector is ready for analysis.
10) As soon as the drift and noise meet the target values during "Measuring Time"
the sequence is complete and the RD sign will come up on the Monitor screen.
[ Manual Start ]
1) Set the parameter on operating parameter-setting screen (Refer to 5-4).
2) Start pumping mobile phase solvent at flow rate
3) Press
key in every 10 seconds to on/off the purge valve for few minutes.
Purge
4) Keep pumping mobile phase solvent to reference cell for about 20 minutes from
the above step 2).
5) Press
key to turn off the valve. Mobile phase solvent flows to sample cell.
Purge
6) Wait until the baseline is stabilized.
7) Press
key to do Auto Zero.
Zero
When using organic solvent, wear safety goggles.
Warning
It is recommended that a sink or the equipment for washing eyes be
installed nearby in case the organic solvent in use comes into contact
with the eye(s) or skin.
When any abnormality, such as liquid leakage, is observed, turn off
Warning
the power immediately.
Unplug the detector from the main power source before opening the cover.
When using flammable organic solvent as mobile phase, be sure making
Warning
necessary arrangement to prevent an accidental ignition (firing) by static
electricity.
key once to call the start-up sequence.
key is pushed, "Measuring Time" will be repeated.
key is pushed,the measurement results will be disregarded and the
to reference cell (Purge On).
1 mL/min
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