SHOKO SCIENCE Shodex RI-504 Operation Manual

Refractive index detector

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Ｏｐｅｒａｔｉｏｎ

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Shodex

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RI-501 Refractive Index Detector.

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CAREFULLY before using

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Summary of Contents for SHOKO SCIENCE Shodex RI-504

Page 1 ＳＴ１５－０００６７－ＣＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＯｐｅｒａｔｉｏｎＭａｎｕａｌＭ...
Page 2 Copyright 2015, SHOKO SCIENC SHOKO SCIENC SHOKO SCIENC SHOKO SCIENCE E E E CO.,LTD CO.,LTD CO.,LTD Printed in Japan. CO.,LTD All rights reserved. Contents of this publication may not be reproduced in any form or by any means(including electronic storage and retrieval or translation into a foreign language)
Page 3 Precautions Thank you for your continued patronage. Observe the following precautions in order to make safe and stable use of the detector. 【 Important ! 】 Precautions are divided into three groups in this operation manual depending on the degree of danger. The three groups are Warning : This sign is used where failure to follow strictly the instructions and procedures indicated by this sign may cause injury or damage...
Page 4 If the fuse is kept blown off after being replaced, power off Warning the detector immediately and disconnect the power cord. Please consult out local representative in your area or SHOKO SCIENCE. Use only the following power supply cord. Using the wrong Caution power cord could result in danger or fire.
Page 5 If the detector is not to be used for more than one week, then, Caution prior to storage, wash the flow line with pure water or acetone, and dry the line by allowing nitrogen gas to flow through. Do not use any eluent which might corrode the material, such as Caution stainless steel, that it comes into contact with.
Page 6: Table Of Contents
Contents １． Accessories １･･･････････････････････････････････２． Features ２･･･････････････････････････････････････３． Specifications ３･････････････････････････････････４． Detection Principle ４････････････････････････････４－１ Optical System ４･･･････････････････････４－２ Flow Line ５･････････････････････････････４－３ Electric Circuit ６･･････････････････５． Nomenclature and Function ８...
Page 7 1. Accessories When unpacking, please check if all the following accessories are contained: Details of Accessories 【】 Name Specification Quantity (1) Voltage is 100 oｒ 120 V AC Power supply cable set (125V) Power cable (2) Voltage is 220 oｒ 240 V AC Power supply cable set (250V) Signal cable 2-core shield cable (approx.
Page 8 2. Features Shodex RI-504 is Deflection Type Refractive Index Detector with following features: 1) Stability The double temperature control system provides for a very fast start up time and excellent stable baseline performance. 2) Safety Solvent leak sensor generates an output signal that stops pump flow in case of an eluent leak within the detector.
Page 9 3. Specifications 1) Construction : Deflection type 2) Refractive Index Range : 1.00 ～ 1.75 3) Range : 0.25 ～ 512 μ RIU 4) Linearity : 600 μ RIU 5) Noise : 5 nRIU or less (Response : 1.5 seconds) 6) Drift : 200 nRIU/ｈ...
Page 10: ４． Detection Principle
4. Detection Principle 4-1 Optical System In a refractive index detector of the deflection type, the light beam undergoes a deflection in proportion to a difference in refractive index between one liquid and the other as it passes through the two compartments of the flow cell, each being filled with a different liquid.
Page 11: ４－２ Flow Line
When a difference develops in the refractive index between the sample and the Reference in the flow cell, the image on the photo sensor moves horizontally as illustrated in Fig. 4.3 below ( moves from (a) to (b) ). The electric signals emitted respectively from the photo sensor change in proportion to the extent of movement of the image.
Page 12: ４－３ Electric Circuit
When the pressure in the flow path has become excessive, relief valve will reduce the pressure. 4-3 Electric Circuit The electrical circuit of Shodex RI-504 consists of two I/V converters, a signal processing circuit (with display and keyboard), a temperature control circuit and a light intensity control circuit.
Page 13 Two photo sensing elements generate a photocurrent proportional to the amount of light. These currents are then converted to the voltage signal in the I/V converters. These voltage signals will then be sent to the signal processing circuit where their Balance will be precisely converted into the digital signal corresponding to the refractive index.
Page 14: ５． Nomenclature And Function
□ □ □ □ □ □ Fig. 5.1 Front panel of Shodex RI-504 Name of parts Function Power Switch Press this key once to turn on or off the unit. □ A 24-digit 2-row character display panel. This is used for LCD Display □...
Page 15: ５－２ Side Panel
Eluent passing through the flow path is discharged from this □ [OUT] port. 5-2 Side Panel □ Fig. 5.2 Side panel of Shodex RI-504 Name of parts Function In case of internal eluent leak, the eluent will be discharged □ Drain Port From this port.
Page 16: ５－３ Back Panel
□ □ □ □ □ □ □ □ Fig. 5.3 Back panel of Shodex RI-504 Name of parts Function Communication port □ LAN communication port [COM] Zero in terminals When these two terminals are short-circuited just as when the zero □...
Page 17: ５－４ Display
Power connector The included power cable should be plugged into □ [～LINE] this connector. These are the terminals to ground the main body Ground terminals □ of the detector. 5-4 Display The display shows a “Monitor screen” when the detector is started and can be Switched to a “Parameter setting screen”...
Page 18 This indicates error or operation status. If there are multiple messages to be displayed, the priority message is displayed. ( 1 ) ROM : Is displayed when a ROM error has occurred. ( 2 ) RAM : Is displayed when a RAM error has occurred. ( 3 ) PARAMETER : Is displayed when a parameter memory error has occurred.
Page 19 Press The Enter key to start the SPAN CHECK. Press The S.Seq key to start and stop the S.SEQ. Press The Enter key to display the result of the validation Each S.SEQ mode is changed with the ENTER key and Arrow Keys The S.SEQ mode is displayed by a screen2.
Page 20 Table 5-1 Settable parameters Parameter Selectable value Unit Default REC. RANGE 0.25，0.5，1，2，4，8，16，32， μRIU/10mV 64，128，256，512 （12 Steps） INTEG. RANGE 128，512 μRIU/1024mV TEMPERATURE OFF，30～55 （1 Step） ℃ TIME CONSTANT 0.1，0.25，0.5，1，1.5，2，3，6 （8 Steps） POLARITY ＋，－－＋ BASELINE SHIFT 0～200 （１ Step 50nRIU）－...
Page 21: ５－５ Start-Up Sequence
５－５ Start-Up Sequence 1）S.SEQ MODE Choose one of the built-in sequences (Fine, Standard, Coarse) from the menu or make own sequence (Custom). Each S.SEQ mode is changed with the ENTER key and Arrow Keys Table 5-2 Parameters (Start- Up Sequence) Parameter FINE STANDARD...
Page 22: ６． Installation And Connection
6. Installation and Connection 6-1 Power Connection and Grounding Connect the detector to the power source according to the following procedure: 1) Confirm that the type of the power socket into which the detector power cable is plugged is of a 3P type with a grounding terminal. 2) Confirm the voltage of the power socket into which the detector power cable is plugged is the same as the voltage indicated on the rear panel of the detector.
Page 23: ６－２ Signal Cable Connections
6-2 Signal cable Connections Make correct connections using the provided signal cables in accordance with the figures below. Connect red wire to ”+” terminal, white wire to”-” terminal and clear wire to “FG”. ) driver ) driver Inserts the end of lead wire while Then, remove the (-) driver to lock the wire.
Page 24: ６－３ Tube Connections
6-3 Tube Connections When connecting this detector to a high performance liquid chromatograph through tubes, wash the chromatograph and columns thoroughly with the eluent in use and make sure that the eluent is clean before connecting the tube. Connect the tubes in accordance with the following procedures : □...
Page 25: ６－４ Operation Procedures
6-4 Operation Procedures Use the detector in accordance with the following procedures: [ Automatic Start up ] 1) Set parameters on the operating parameter-setting screen. 2) Set parameters on the start-up sequence parameter-setting screen. 3) Start pumping the eluent at a flow rate of 1 mL/min. If pumping can be done only at a flow rate lower than 1 mL/min, a longer purge cycle needs to be set.
Page 26 If any eluent freezes inside the detector, the flow line might break Caution damaging the detector. If there is any chance of the eluent freezing during stoppage or storage of the detector, withdraw the eluent completely from the flow line of the detector. When using any eluent containing a salt of high concentration, Caution make sure that the flow line is washed with water thoroughly after use.
Page 27: ７． Care And Maintenance
Please consult our local representative in your area or Warning SHOKO SCIENCE CO.,LTD. 7-3 Cleaning of detector exterior When the exterior such as top cover of front panel were stained or got wet for whatever reasons, please keep the detector clean and dry by wiping off it by soft cloths or tissue paper.
Page 28: ８． Validation
It is recommended that the HPLC system be validated from time to time to maintain the accuracy and reliability of analysis. Thin task of validation has been made easier due to the unique and user-friendly feature of Shodex RI-504. Press the key to obtain the “SPAN VALIDATION”...
Page 29 2) Equilibrate Shodex RI-504 by pumping deionized water through the reference and sample cells. Use the same deionized water as that used to prepare the sucrose standard solution. Start pumping at a flow rate of 1mL/min and do the start-up sequence.
Page 30: ９． Troubleshooting
9. Troubleshooting If any abnormality occurs, try to solve the problem in accordance with the Instructions below. Should the problem not be corrected even after taking the corrective actions below, contact the agent from whom you purchased the detector. Problem Possible cause Solution No power...
Page 31 Problem Possible cause Solution Large baseline drift 1. Insufficient solvent 1. Replace the solvent thoroughly. replacement. (Refer to the notes in 6-4.) 2. Room temperature 2. Use detector in places where changes fluctuates greatly. in room temperature are small. 3. Bubbles are present in 3.
Page 32: Weee Instructions
10. WEEE Instruction Instrument marked with this mark indicates that it was sold on ＷＥＥＥＭａｒｋ or after 13th August 2005, and it is covered by Waste Electrical and Electronic Equipment (WEEE) Directive. This WEEE mark means it must be collected separately from general household waste, according to the regulatory guideline in your area.
Page 33: Appendix 1 「Property Data Of Solvents
Appendix 1 Property Data of Solvents Flash Boiling Fire Point Polarity Viscosity Refractive Vapor Cut-off Point Point Gravity Index Density (cP20℃) (nm) (℃) (℃) Lower Upper (℃) Fluoroalkanes -0.25- 1.250 n-Pentane 0.00 0.23 1.358 <-40 308.9 07.8 036.1 Hexane 0.00 1.375 -21.7 233.9...
Page 34: Appendix 2 「Components And Subassemblies
Appendix Appendix 2 2 2 2 Appendix Appendix Shodex RI RI RI RI- - - - 5 5 5 5 0 0 0 0 4 4 4 4 Components and Subassemblies Components and Subassemblies Components and Subassemblies Components and Subassemblies Part Number Part Number Part Number...
Page 35 Shodex RI-504 Refractive Index Detector or granting or implying any license or immunity under any patent or other rights. Shodex RI-504 Refractive Index Detector must be used on user's own responsibility and in strict compliance with all applicable laws and regulations.
Page 36 SHOKO SCIENC SHOKO SCIENCE E E E CO., LTD. SHOKO SCIENC SHOKO SCIENC CO., LTD. CO., LTD. CO., LTD. ＦａｃｔｏｒｙＤｅｐａｒｔｍｅｎｔ 1-3-3, Azamino-Minami, Aoba-ku, Yokohama City, Kanagawa 225-0012, Japan TEL : +81-(0)45-913-5544 FAX : +81-(0)45-913-5802 Email : tu.tokieda@shoko-sc.co.jp http : www.shoko-sc.co.jp...

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