To Verify Sensitivity For 6130 And 6140 Quads - G1978B Multimode, Positive Scan Modes - Agilent Technologies G1978B Setup Manual

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Positive Scan Modes

To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode,
Positive Scan Modes
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
2 Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium
3 Transfer 5 mL of the first dilution to a 50 mL volumetric flask. Use a clean
4 Dilute to the 50 mL mark with 75:25 methanol/water with 5 mM ammonium
5 Transfer approximately 1 mL of the second dilution to an autosampler vial.
Table 3
Sample
Concentration after dilution
Injection volume
Total sample amount injected
Sample order number
Solvent
Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
flask. Use a clean graduated pipette.
formate.
graduated pipette.
formate.
This provides the final 10 pg/µL reserpine concentration required for
performance verification.
6130 and 6140 Quad Performance Verification Summary, Scan Mode
G1978B
ES Positive Scan Mode
Reserpine, 5 ng/µL
10 pg/µL
5 µL
50 pg
G2423A
75:25
methanol/water
with 5mM
ammonium formate
Verification
G1978B
APCI Positive Scan Mode
Reserpine, 5 ng/µL
10 pg/µL
5 µL
50 pg
G2423A
75:25
methanol/water
with 5mM
ammonium formate
2
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