To Verify Sensitivity For 6120 Quad - G1978B Multimode, Positive Sim Modes - Agilent Technologies G1978B Setup Manual

2 Verification

To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes

To verify sensitivity for 6120 Quad - G1978B Multimode, Positive
SIM Modes
The G6110A instrument does not support the multimode source without the Selection
N O T E
valve. Use the option kit G2735H to enable the G6110A to work with the multimode source.
Preparation
1 Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric
2 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
3 Transfer 1 mL of the first dilution to a second 50 mL volumetric flask. Use a
4 Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or
5 Transfer approximately 1 mL of the second dilution to a vial for use in the
Table 1
Sample
Concentration after dilution
Injection volume
Total sample amount injected
Sample order number
Solvent
22
flask. Use a clean graduated pipette.
acetic acid.
clean graduated pipette.
acetic acid.
This provides the final 2 pg/µL reserpine concentration required for
performance verification.
LC autosampler.
6110 and 6120 Quad Performance Verification Summary Table
Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
G1978B
ES Positive SIM Mode
Reserpine, 5 ng/µL
2 pg/µL
5 µL
10 pg
G2423A
75:25
methanol/water
with 0.1% formic or acetic acid
G1978B
APCI Positive SIM Mode
Reserpine, 5 ng/µL
2 pg/µL
5 µL
10 pg
G2423A
75:25
methanol/water
with 0.1% formic or acetic acid