Sample Clumpy Mcf7 Cells; Disabling Decluster For Small Cells - Nexcelom Bioscience Cellometer Auto T4 User Manual

SAMPLE CLUMPY MCF7 CELLS

In the sample below, clumpy MCF7 cells are analyzed using both default and modified Decluster Edge Factor and
Decluster Th Factor parameter settings.
MCF7 cell count results were analyzed using different "Decluster" parameter settings. Figure (a) shows default decluster
settings (cells within clumps are not fully separated), resulting in MCF7 concentration of 6.5 x 105 cell/mL. When
modified declustering settings are used as shown in figure b, cells within the clumps are separated resulting in MCF7
concentration of 8.3 x 105 cell/mL.

DISABLING DECLUSTER FOR SMALL CELLS

Examples of small size cells are mouse splenocytes,
lymphocytes, thymocytes, and PBMCs. Their cell diameters
are typically less than 7 µm.
The Auto T4's focus position for really small cells may
influence cell counting when the Decluster feature is
enabled (i.e., the Do not Decluster Clumps check box
is not selected). This feature works in conjunction with the
Contrast Enhancement value.
For the purpose of the following example, cell type
parameter settings as shown in this screen were used
to count mouse thymocyte cells in low concentration
(i.e., cell counts less than 1300). Images explaining
count results for various scenarios are presented.
8001009 Cellometer® Auto T4 User Manual Rev C
Edge Factor = 0.5
Th Factor = 1.0
Chapter 9. Cell Types
Edge Factor = 0.7
Th Factor = 0.8
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