Thermo Scientific PB5034A Manual

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Oxoid Prepared Medium
Oxoid Schaedler Anaerobe Agar with Sheep
Blood (with Haemin and Vitamin K1)
PB5034A
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Intended Use
Thermo Scientific™ Oxoid™ Schaedler Anaerobe Agar with Sheep
Blood (with Haemin and Vitamin K1) is a non-selective medium for the
isolation of obligate and facultative anaerobic bacteria.
For professional use only (in vitro diagnostic use).
Summary and Explanation
Schaedler, Dubos and Costello
isolation of aerobic and anaerobic microorganisms from the gastro-
intestinal tract of mice. Mata, Carrillo and Villatoro
formula in their studies on anaerobic human faecal microflora. This
modified formula has been used in Oxoid Schaedler Anaerobe Agar
with Sheep Blood (with Haemin and Vitamin K1). The medium is used
as a non-selective, highly nutritious agar for the isolation of
anaerobes.
Principle
Tryptone soya broth and special peptone supply nitrogen and other
nutrients necessary for growth. Yeast extract supplies vitamins and
other growth factors. Agar is added as a solidifying agent. Glucose is
a carbohydrate source and acts as a reducing agent. Haemin is
required by a variety of obligate anaerobes. Tris buffer prevents an
extreme drop in pH during glucose fermentation. Cysteine acts as a
reducing agent and stimulates the growth of some anaerobes. Vitamin
K is a growth requirement for some strains of Prevotella
melaninogenica and enhances the growth of some Bacteroides
fragilis and Gram-positive non-spore formers. Sheep blood supplies
haemin and additional growth promoting substances.
Typical Formula*
Tryptone soya broth
Special peptone
Yeast extract
Glucose
Sodium chloride
Cysteine HCl
Haemin
Tris buffer
Agar
Additions
Vitamin K1
Defibrinated sheep blood
* Adjusted as required to meet performance standards
Physical Characteristics
Colour Signal red
Clarity Opaque
Fill weight 17 g ± 5%
pH 7.6 ± 0.2
Precautions
This product is for in vitro diagnostic use and should only be used by
trained individuals. This includes the disposal of used or unused
reagents as well as any other contaminated disposable material
following procedures for infectious or potentially infectious products. It
BT-IFU-0086
1
formulated this medium for the
2
modified the
grams per litre
10.0
5.0
5.0
5.0
4.0
0.4
0.01
0.75
13.5
per litre
0.002 g
50.0 mL
is the responsibility of each laboratory to manage waste produced
according to their nature and degree of hazard and to have them
treated or disposed of in accordance with any federal, state and local
applicable regulations.
carefully.
For professional use only. Safety Data Sheet available on request.
Storage
This product is ready to use and no further preparation is necessary.
Store product in its original packaging at 2‒12°C until used.
Store away from light.
Allow product to equilibrate to room temperature before use. Do not
incubate prior to use.
Specimen Collection, Handling and Storage
Specimens should be collected and handled following the
recommended guidelines.
Materials Required but Not Supplied
(1) Inoculating loops, swabs, collection containers
(2) Incubators
(3) Quality control organisms
More information can be found at www.thermofisher.com
Procedure
Refer to any local protocols and guidelines.
(1) Inoculate and streak the specimen onto the medium.
(2) If material is being cultured directly from a swab, roll the swab
over a small area surface and streak for isolation of single
colonies.
(3) Immediately incubate plates anaerobically at 36°C ± 1°C for 48
hours. Plates can be examined for growth after 24 hours.
Prolonged incubation may be required for some anaerobes.
Quality Control
This medium can be tested with the following strains:
Incubation Conditions: 40 – 48 h @ 36 ± 1°C, anaerobic
Positive Controls
Inoculum of 50 – 120 colony forming units (cfu).
Bacteroides fragilis
®
ATCC 25285
Peptostreptococcus
anaerobius ATCC
Colony counts shall be greater than or equal to 50% of the
control medium (Columbia agar with sheep blood).
Inoculum of 10
Clostridium perfringens
®
ATCC 13124
Note:
It is the responsibility of the user to perform Quality Control testing
taking into account the intended use of the medium, and in
accordance with any local applicable regulations (frequency, number
of strains, incubation temperature etc.).
The product should not be used if:
(1) The product is contaminated
(2) The colour has changed
(3) The expiration date has passed
(4) There are other signs of deterioration
Performance
Performance was evaluated using 18 bacterial strains including the
following: Clostridium spp., Bacteroides spp., Peptostreptococcus
spp., Prevotella spp., Staphylococcus aureus and Enterococcus
faecalis. All organisms gave expected growth characteristics
according to the current product specification.
Page 1 of 2
Directions should be read and followed
2 – 4 mm, white shiny
colonies
2 – 4 mm, white shiny
®
27337 colonies
4 5
– 10 cfu.
Good growth, cream
colonies with double zone
of haemolysis