EIJKELKAMP Aquaprobe AP-5000 Instruction Manual
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EIJKELKAMP Aquaprobe AP-5000 Instruction Manual

Multiparameter water quality probe
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Multiparameter Water Quality Probe
Nijverheidsstraat 30,
6987 EM Giesbeek, The Netherlands
T +31 313 880 200
E info@eijkelkamp.com
I
www.eijkelkamp.com
Instruction Manual
Aquaprobe
and associated
Aquameter
®
Aquameter
®
firmware Version 6.20 and Above
AP-5000 firmware Version 4.07 and Above
Document No. 10401-00906
Revision: L
Date: March 27, 2017
for the
AP-5000
®
, Utilities & Accessories
M-1858E

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Summary of Contents for EIJKELKAMP Aquaprobe AP-5000

  • Page 1 , Utilities & Accessories Aquameter ® firmware Version 6.20 and Above AP-5000 firmware Version 4.07 and Above Document No. 10401-00906 Revision: L Date: March 27, 2017 Nijverheidsstraat 30, 6987 EM Giesbeek, The Netherlands T +31 313 880 200 E info@eijkelkamp.com M-1858E www.eijkelkamp.com...

  • Page 2: What's New In This Manual

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® PROPRIETARY NOTICE The information disclosed within this document or separately supplied in furtherance of this document includes proprietary rights of Aquaread ® Ltd. Neither this document nor the information disclosed herein or of a supplementary nature shall be reproduced or transferred to other documents or used for any other purposes, except as specifically authorised in writing by Aquaread ®...
  • Page 3 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® What's New in this Manual? This Manual, Revision L, covers the new features introduced in 2017 with AP-5000 firmware version 4.07 and Aquameter ® firmware version 6.20. Aquameter ® and Aquaprobe ® Firmware ➢...

  • Page 4: Table Of Contents

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Table of Contents WHAT'S NEW IN THIS MANUAL?..........................3 1. INTRODUCTION................................10 2. WHAT’S IN THE BOX?...............................10 2.1. T ® ........................10 QUAMETER AND THE NVIRONMENT 2.2. T AP-5000 ...........................10 AND THE NVIRONMENT 2.3. A ......................10 BOUT THE ROBE...
  • Page 5 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 12. GENERAL PROBE MAINTENANCE........................34 12.1. I ........................34 DENTIFYING NDIVIDUAL LECTRODES 13. PH/ORP ELECTRODE CALIBRATION AND MAINTENANCE................35 13.1. R H/ORP E ........................35 ECOGNISING THE P LECTRODE 13.2. E ........................35 LECTRODE EMOVAL AND EPLACEMENT 13.3.
  • Page 6 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.6.4. Calibration Solution Preparation........................63 15.6.5. Serial Dilution..............................63 15.6.6. Zero Point Calibration...........................64 15.6.7. Calibrating Point 2............................65 15.6.8. Calculating and Applying a Grab Sample Factor..................65 15.6.9. Errors During Calibration..........................66 15.6.10. Lens and Sleeve Maintenance........................66 15.7.
  • Page 7 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16. OPTIONAL ISE ELECTRODES CALIBRATION AND MAINTENANCE............92 16.1. ISE E ............................92 LECTRODE IMITATIONS 16.2. C ..............................92 ALIBRATION OINTS 16.3. S ISE E ......................92 PECIAL OTES ONCERNING LECTRODES 16.4. 5000/7000-AMM A ....................93 MMONIUM MMONIA LECTRODE 16.4.1.
  • Page 8 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 18. LIMITED WARRANTY............................121 18.1. C ............................121 LEANING RIOR ETURN 18.2. D ..........................122 ECONTAMINATION ERTIFICATE 19. TROUBLESHOOTING.............................123 20. DECLARATION OF CONFORMITY........................124 21. APPENDIX 1. THE TECH BEHIND AQUAREAD’S OPTICAL DO MEASUREMENT SYSTEM....125 21.1. P ............................125 RINCIPLE OF PERATION...

  • Page 9: Introduction

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 1. Introduction This manual covers the setup, operation, calibration and maintenance of the Aquaprobe ® AP-5000 V4.07+, Aquameter ® V6.20+ Meter, AquaLink V5.00+ PC software and associated Aquaprobe ® accessories. If your Aquaprobe ®...

  • Page 10: Top Tips For Successful Measurements Using Optical Electrodes

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Probe sleeves are also specifically matched to the Probe with which they were supplied. If you have more than one AP-5000, be sure not to mix the sleeves up between the Probes. Doing so may seriously affect the performance of the EC electrode. All Aquaread ®...

  • Page 11: Battery Installation And Care

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 3. Battery Installation and Care The Aquameter ® requires five AA size batteries. To install the batteries, loosen the two screws on the centreline of the rear of the meter and remove the battery compartment lid. Following the battery polarity markings inside the battery compartment, insert five AA cells then replace the compartment lid and tighten the screws.

  • Page 12: Overview Of The Operating System

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 4. Overview of the Operating System The operating firmware in the Aquameter ® has been designed for simple, intuitive use. Similarly, a great deal of development work has been put into simplifying and automating the calibration procedures in the Aquameter ®...

  • Page 13: Connecting An Ap-5000

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 5. Connecting an AP-5000 The AP-5000 is designed to connect to the Aquameter ® using an AP-5000 Extension Cable. The AP-5000 Extension Cable features high-pressure metal connectors, which incorporate several O-ring seals at the Probe end. Prior to first connection, the seals must be lubricated using the silicone grease supplied.

  • Page 14: Taking Measurements

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 6. Taking Measurements The AP-5000 includes a pH/ORP electrode, which is kept moist by a storage cap. Remove the storage cap by pulling the red lanyard marked ‘Remove Before Use / Replace After Use’...

  • Page 15: What Does It All Mean

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ®  Lat:N51 ° 21.498 ° Long:E001 24.323 Alt:00050M 1013mb Sats in use:09   6.1. What Does It All Mean? The screens above show the full default range of readings for the AM-200/AP-5000 combination.

  • Page 16: Temperature Compensation

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® When taking a set of readings, gently stir the Probe, or raise and lower it in the sample (if there is no natural water flow) until the global stability icon appears. The initial display of the global stability icon will be accompanied by a double beep.

  • Page 17: Gps Reception

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 6.5. GPS Reception The GPS version of the Aquameter ® (AM-200) contains a built-in GPS/GLONAS receiver and antenna. The antenna is situated at the top of the case, just behind the AQUAREAD Logo.

  • Page 18: Depth Measurement

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 7. Depth Measurement Depth is measured in the AP-5000 by a pressure sensor mounted inside the body of the probe. The datum on the probe for depth measurement is the top row of holes on the probe sleeve.

  • Page 19: Memory Mode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 8. Memory Mode 8.1. Manually Saving Readings When you are happy that the readings are stable (see section 6.3: Global Stability Indication), press the M+ key to snapshot the readings along with the time, date, GLP (calibration) data and position (GPS models only).

  • Page 20: Clearing The Memory

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® PH 7.00[31/Jan/17] PH 4.01[07/Feb/17] PH 10.0[07/Feb/17] ORP+250[09/Feb/17] This tells you that the last successful calibration, prior to the recorded reading being taken, was January 31 for the pH 7.00 point, February 7 for the pH 4.01 &...

  • Page 21: Battery And Memory Duration In Low Power Logging Mode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select Auto Data Logging by pressing the down arrow key then the right arrow key or the OK key. The Auto Data Logging screen will be displayed. Auto Data Logging Interval:10 Mins ...

  • Page 22: Important Information About Memory Mode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Logging Rate Battery Life (at 21°C) Memory Duration 90 mins 37 Days 625 Days 60 mins 34 Days 416 Days 45 mins 32 Days 312 Days 30 mins 28 Days 208 Days 15 mins 20 Days 104 Days...

  • Page 23: Setup & Install

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 9. Setup & Install To alter the way the Aquameter ® displays readings, press the MENU key to get to the Main Menu, then choose Setup & Install. The Settings Menu will be displayed. Please note, the 'Socket Assignment' option on this screen is only accessible when an AP-5000 Probe is connected.

  • Page 24: Aux Socket Assignment

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Selecting the second line of the Units Menu will display the following screen. Units TEMP: °  pH:pH SAL:PSU The first option on this screen allows you to change the temperature display between C and F.
  • Page 25 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® When the Socket Assignment option has been selected, the following screen will be displayed. The numbers 1 – 6 represent the AUX socket numbers. Only sockets 1 -4 are available on the AP-5000 so the other two are shown as N/A. These additional sockets are available on the AP-7000 only.

  • Page 26: Rapidcal Calibration Method

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 10. RapidCal Calibration Method 10.1. About Calibration Calibration is a very important part of successful water quality measurement and should be carried out regularly as detailed in each separate section of this manual. A great deal of development work has been put into simplifying and automating the calibration procedures in the Aquameter ®...
  • Page 27 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 1. Pour 300mL of RapidCal solution into one of the calibration cups provided as shown in the adjacent photograph. 2. Remove the storage cap from the pH electrode if fitted, wash the Probe in distilled water, then gently lower the Probe into the calibration cup.

  • Page 28: To Check / Calibrate The 100% Saturation Point In Damp Air

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Important If you have ISE electrodes fitted to your Aquaprobe ® , you must now remove the rubber sealing caps from all the ISE electrodes. Now the DO 100% saturation point should be checked and if necessary, calibrated in damp air.

  • Page 29: Calibration Error Messages

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 10.4. Calibration Error Messages If the Aquameter ® detects a problem with either the AP-5000 or the calibration solution during the calibration procedure, an error will be indicated. The chart below shows the possible errors and how to correct them.

  • Page 30: Resetting To Factory Calibration Defaults

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 10.5. Resetting to Factory Calibration Defaults In some cases, if there has been a serious calibration error, the easiest way to rectify the situation is to reset the Probe to its factory defaults. To do this, first bring up the Calibration screen: Calibration RapidCal...
  • Page 31 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® In order to recall the calibration data for a certain electrode on the Meter, first select the calibration screen for that electrode. If, for instance, you selected pH/REDOX(ORP), the screen below would be displayed. pH7.00 [31/Jan/17] pH4.01 [07/Feb/17] PH10.0 [07/Feb/17]...

  • Page 32: After Use

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 11. After Use The AP-5000 should always be cleaned after every use. It is advisable to clean the Probe after use with the cable attached. This will prevent any water entering the Probe's socket and will allow any deposits to be removed from the connector collar and shell.

  • Page 33: General Probe Maintenance

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 12. General Probe Maintenance Other than regular cleaning and calibration, very little in the way of maintenance is needed. 12.1. Identifying The Individual Electrodes The photograph below shows the two standard electrodes, the Combi pH/ORP electrode and the DO/EC electrode.

  • Page 34: Ph/Orp Electrode Calibration And Maintenance

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 13. pH/ORP Electrode Calibration and Maintenance 13.1. Recognising the pH/ORP Electrode The combined pH/ORP electrode is easy to recognise because it is the only electrode that is not black. This electrode has a clear, gel filled body. 13.2.

  • Page 35: Calibrating Ph

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 13.4. Calibrating pH pH electrodes should be calibrated fully at least once a week to ensure optimum accuracy. Full calibration involves calibrating at pH 7.00 first, then at pH 4.01 and/or pH 10.00. The AP-5000 allows for both two and three point pH calibration.
  • Page 36 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 6. Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes 7. Select pH/ORP. The screen will change to: pH 7.00?[01/Jan/17]  PH 4.01?[01/Jan/17] PH 10.0?[01/Jan/17] ORP+?250[01/Jan/17] The dates shown to the right of the screen are the dates of the last successful calibration. 8.

  • Page 37: Calibrating The Second Point

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 13.5.2. Calibrating the Second Point The pH electrode can now be calibrated at either pH 4.01 or pH 10.00. If you intend to calibrate at both pH 4.01 and pH 10.00, both points must be calibrated in the same session, i.e.

  • Page 38: Calibrating Orp

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Never place the entire AP-5000 in methyl alcohol, as this will cause irreparable damage to the DO/EC electrode. Damaged caused in this way is not covered by the warranty. If the methyl alcohol rinse does not restore the electrode, perform the following actions: Remove the electrode from the body again.
  • Page 39 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select pH/REDOX(ORP). The screen will change to: pH 7.00?[01/Jan/17]  PH 4.01?[01/Jan/17] PH 10.0?[01/Jan/17] ORP+250?[01/Jan/17] Move the arrow to the bottom line. If you are using 250mV calibration solution press the OK key to continue. If you are using 229mV calibration solution, move the cursor to the right, then use the up/down arrow keys to select +229.

  • Page 40: Converting Orp Readings To The Hydrogen Scale

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 13.10. Converting ORP Readings to the Hydrogen Scale Electrochemical measurements are ultimately referred to the so-called hydrogen scale, the convention for which is that the electrochemical potential of a hydrogen electrode in contact with hydrogen gas at one atmosphere partial pressure and a solution containing hydrogen ions at unit activity is zero at all temperatures.

  • Page 41: Do/Ec Electrode Calibration And Maintenance

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 14. DO/EC Electrode Calibration and Maintenance 14.1. Recognising the DO/EC Electrode The DO/EC electrode is easy to recognise because it has a screw-on cap and four stainless-steel EC sensor contacts on the side (see photograph in section 12). Dissolved Oxygen (DO) is measured at the end of the electrode by the components behind the removable cap.
  • Page 42 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes 6. Select DO/EC. The screen will change to: Calibration DOZero?[01/Jan/17]  DO100%?[01/Jan/17] EC2570?[01/Jan/17] The dates shown to the right of the screen are the dates of the last successful calibration.

  • Page 43: Calibrating The Do 100% Saturation Point In Moist Air

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® If a problem occurs during calibration, an error message will be displayed. Refer to Calibration Error Messages in section 10 for error message handling. Remove the Probe from the calibration cup, rinse thoroughly in fresh water, shake off any excess and dry the outer sleeve with a soft cloth.
  • Page 44 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 4. Remove the new Optical DO Cap from its light-proof bag and quickly screw it onto the end of the DO/EC electrode. Ensure that the cap is screwed fully onto the electrode and that it is done up tight. 5.

  • Page 45: Calibrating Ec

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 14.8. Calibrating EC EC calibration is always carried out at a single point. There is a choice of two pre-set calibration standards or you can enter any calibration standard value between 100µS/cm and 99,999µS/cm manually.
  • Page 46 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select DO/EC. The screen will change to: Calibration DOZero?[01/Jan/17]  DO100%?[01/Jan/17] EC R-CAL?[01/Jan/17] The dates shown to the right of the screen are the dates of the last successful calibration. The value shown on the bottom line next to ‘EC’ is the value the EC electrode was last calibrated to.

  • Page 47: Verifying Ec Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Calibration Report on the top line displays the EC Cell Constant. This value is stored in the Probe's memory and can be recalled at any time. See section 10.7. Calibration Data Storage and Retrieval Press OK then ESC repeatedly to return to normal reading mode.

  • Page 48: Calibrating Temperature

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 14.12. Calibrating Temperature The Aquaprobe's temperature sensor is built into the oval resin pocket located on the back of the DO/EC sensor. The temperature sensor is extremely linear and by default is set up to read within +/- 0.5°C of the true temperature, which is ample for most applications.

  • Page 49: Optional Optical Electrodes Calibration And Maintenance

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15. Optional Optical Electrodes Calibration and Maintenance The AP-5000 is constructed with an aluminium sleeve surrounding the delicate sensing electrodes. The Sleeve can be easily removed by unscrewing to allow cleaning of the individual electrodes, however, the Probe sleeve forms an integral, working part of the Probe’s measurement system, and MUST be fitted for correct operation.

  • Page 50: Optical Electrode Calibration Sequence

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.2. Optical Electrode Calibration Sequence Optical electrodes feature either two or three point calibration, dependent upon the type. In all cases however, the lower calibration points is ZERO. When calibrating any optical electrode, the Zero point must be calibrated first. If you are performing a two or three point calibration, all calibration points must be calibrated within the same calibration session (i.e.

  • Page 51: 2000/5000-Turb Turbidity Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.4. 2000/5000-TURB Turbidity Electrode Turbidity can be measured by the AP-5000 using the optional 2000/5000-TURB optical electrode. This electrode employs a Nephelometric technique in accordance with ISO 7027, which uses Formazin as a reference standard. The Aquameter ®...

  • Page 52: Negative Turbidity Readings

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.4.3. Negative Turbidity Readings When a Probe is deployed in clean/clear water and negative turbidity readings occur, the cause is usually an erroneous zero point calibration, caused either by contaminated calibration solution, aeration or changes in the measurement chamber between zeroing and deployment.

  • Page 53: Turbidity Zero Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.4.6. Turbidity Zero Point Calibration To calibrate the Turbidity zero point (zero the electrode), follow these steps: 1. Put at lease 300mL of clean water in a calibration tube (bottled still mineral water is recommended), remove the storage cap from the pH electrode if fitted, wash the Probe in clean water, then drop the Probe in all the way.

  • Page 54: Verifying The Zero Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select ZERO. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up.

  • Page 55: Errors During Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Gently pour 300mL of the solution into a clean calibration tube and drop the Probe in all the way. Again, agitate and swirl the Probe and bottle several times in order to remove any air bubbles that may be clinging to the Turbidity electrode.

  • Page 56: 2000/5000-Bga-Pc Freshwater Blue-Green Algae (Phycocyanin) Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.5. 2000/5000-BGA-PC Freshwater Blue-Green Algae (phycocyanin) Electrode Freshwater Blue-Green Algae (BGA-PC) can be measured by the AP-5000 using the optional 2000/5000-BGA-PC optical electrode. 15.5.1. Principle of Operation The 2000/5000-BGA-PC optical electrode is a submersible, fixed response fluorometer, which provides excitation at 590nm and detects any resultant fluorescence above 655nm.
  • Page 57 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.5.4. Calibration Solution Preparation In order to 'calibrate' (actually, set the relative sensitivity) of the BGA-PC electrode, a 100µg/L calibration solution of fluorescent dye known as Rhodamine WT should be used. This is exactly the same calibration solution that is recommended for calibration of the RHOD electrode.
  • Page 58 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.5.6. Zero Point Calibration To calibrate the zero point, follow these steps: 1. Pour 300mL of clean water (bottled still mineral water is recommended) into a clean calibration cup, remove the storage cap from the pH electrode if fitted, wash the Probe in clean water, then gently lower the Probe in all the way.
  • Page 59 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up.
  • Page 60 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® To calculate a GS Factor, first take measurements using the fully calibrated electrode. Next, compare the average of these values with the average values derived by laboratory analysis of grab samples from the same location. To do this, divide the average grab sample value by the average electrode value.
  • Page 61 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® phycoerythrin 15.6. 2000/5000-BGA-PE Saltwater Blue-Green Algae ( ) Electrode Salt-water Blue-Green Algae (BGA-PE) can be measured by the AP-5000 using the optional 2000/5000-BGA-PE optical electrode. 15.6.1. Principle of Operation The 2000/5000-BGA-PE optical electrode is a submersible, fixed response fluorometer, which provides excitation at 520nm and detects any resultant fluorescence above 575nm.
  • Page 62 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.6.4. Calibration Solution Preparation In order to 'calibrate' (actually, set the relative sensitivity) of the BGA-PE electrode, an 8µg/L calibration solution of fluorescent dye known as Rhodamine WT should be used. Please note: there is no direct correlation between Rhodamine concentration and the number of BGA-PE cells/mL.
  • Page 63 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.6.6. Zero Point Calibration To calibrate the zero point, follow these steps: 1. Pour 300mL of clean water (bottled still mineral water is recommended) into a clean calibration cup, remove the storage cap from the pH electrode if fitted, wash the Probe in clean water, then gently lower the Probe in all the way.
  • Page 64 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select ZERO. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up.
  • Page 65 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® To calculate a GS Factor, first take measurements using the fully calibrated electrode. Next, compare the average of these values with the average values derived by laboratory analysis of grab samples from the same location. To do this, divide the average grab sample value by the average electrode value.
  • Page 66 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.7. 2000/5000-CPHYLL Chlorophyll Electrode Chlorophyll can be measured by the AP-5000 using the optional 2000/5000-CPHYLL optical electrode. 15.7.1. Principle of Operation The 2000/5000-CPHYLL optical electrode is a submersible, fixed response fluorometer, which provides excitation at 470nm and detects any resultant fluorescence above 630nm. The electrode induces the chlorophyll to fluoresce, then measures the longer wavelength light which is emitted as a result of the fluorescence process.
  • Page 67 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.7.4. Calibration Solution Preparation In order to 'calibrate' (actually, set the relative sensitivity) of the CPHYLL electrode, a 500µg/L calibration solution of fluorescent dye known as Rhodamine WT should be used. Please note: there is no direct correlation between Rhodamine concentration and the concentration of chlorophyll.
  • Page 68 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.7.6. Zero Point Calibration To calibrate the zero point, follow these steps: 1. Pour 300mL of clean water (bottled still mineral water is recommended) into a clean calibration cup, remove the storage cap from the pH electrode if fitted, wash the Probe in clean water, then gently lower the Probe in all the way.
  • Page 69 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select ZERO. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up.
  • Page 70 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® To calculate a GS Factor, first take measurements using the fully calibrated electrode. Next, compare the average of these values with the average values derived by laboratory analysis of grab samples from the same location. To do this, divide the average grab sample value by the average electrode value.

  • Page 71: Calibration Solution Preparation

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.8. 2000/5000-RHOD Rhodamine Electrode Rhodamine WT is a fluorescent red dye that is commonly used in water flow studies and can be measured by the AP-5000 using the optional 2000/5000-RHOD optical electrode. 15.8.1.

  • Page 72: Serial Dilution

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Part number: 70301027 Description: Rhodamine WT Liquid Supplier: Keystone Europe Ltd. Contact: http://www.dyes.com Be sure to handle chemicals with care and to read and comply with all health and safety advice. 15.8.5. Serial Dilution The recommended Rhodamine solution is supplied as a 20% or 200g/L solution, dilution of the stock solution should be carried out as follows.
  • Page 73 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes Select Aux Electrodes. The screen will change to: SELECT ELECTRODE l 4:EMPTY 1:Rhod  l 5:N/A 2:EMPTY l 6:N/A 3:EMPTY The Rhod electrode should have been assigned to an AUX socket when it was fitted.
  • Page 74 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Calibration Report on the top line displays the voltage output from the electrode in millivolts (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7.
  • Page 75 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.9. 2000/5000-FSCEIN Fluorescein Electrode Fluorescein is a fluorescent dye that is commonly used in water flow studies and can be measured by the AP-5000 using the optional 2000/5000-FSCEIN optical electrode. 15.9.1. Principle of Operation The 2000/5000-FSCEIN optical electrode is a submersible, fixed response fluorometer, which provides excitation at 470nm and detects any resultant fluorescence above 550nm.
  • Page 76 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Part number: 801 073 81 Description: Keyacid Fluorescein 019187 Supplier: Keystone Europe Ltd. Contact: http://www.dyes.com Be sure to handle chemicals with care and to read and comply with all health and safety advice. 15.9.5.
  • Page 77 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes Select Aux Electrodes. The screen will change to: SELECT ELECTRODE l 4:EMPTY 1:Fcein  l 5:N/A 2:EMPTY l 6:N/A 3:EMPTY The Fcein electrode should have been assigned to an AUX socket when it was fitted.
  • Page 78 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.9.7. Calibrating Point 2 Remove the Probe from the calibration cup, shake off any excess water then dry the outer sleeve with a soft cloth. Pour 300mL of freshly mixed Fluorescein calibration solution into a clean calibration cup then gently lower the Probe in all the way.
  • Page 79 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.10. 2000/5000-REFOIL Refined Oil Electrode can be measured by Refined fuels such as benzene, toluene, ethylbenzene, and xylenes (BTEX) the AP-5000 using the optional 2000/5000-REFOIL optical electrode. 15.10.1. Principle of Operation The 2000/5000-REFOIL optical electrode is a submersible, fixed response fluorometer, which provides excitation at 285nm (deep UV) and detects any resultant fluorescence between 330nm and 370nm.
  • Page 80 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Fluorescence measurement techniques are ideal for researchers who are interested in detecting the presence or absence of a specific substance and measuring relative fluorescence changes that can be used as an indication of increasing or decreasing concentrations.

  • Page 81: Zero Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® One step process: Weigh out 10.5mg of the recommended salt and add to 1L of deionized water in a volumetric flask. Invert or mix until all salt has dissolved. This gives the Pt-2 10ppm stock solution required for calibration.

  • Page 82: Calibrating Point 2

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select Aux Electrodes. The screen will change to: SELECT ELECTRODE l 4:EMPTY 1:R-Oil  l 5:EMPTY 2:EMPTY l 6:EMPTY 3:EMPTY The Oil electrode should have been assigned to an AUX socket when it was fitted. Choose that socket.

  • Page 83: Calculating And Applying A Grab Sample Factor

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Follow the procedure detailed above for Zero point calibration as far as step 6, then select Pt-2. Wait while the Meter stabilises and calibrates. After successful calibration, the ‘Calibrating 100%’ screen will be displayed along with the Calibration Report, which will show the voltage output from the electrode in millivolts (mV).
  • Page 84 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Never use an abrasive cleaner on the inside of the Probe sleeve or cap as they have been treated with a non-reflective coating which can be easily damaged. The inside of the sleeve should be wiped over with a soft damp cloth and non-abrasive detergent. Always re-calibrate the zero point after cleaning the sleeve or lenses.

  • Page 85: Principle Of Operation

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 15.11. 2000/5000-CDOM/FDOM Chromophoric (Fluorescent) Dissolved Organic Matter Fluorescent) Dissolved Organic Matter can be measured by the AP-5000 using the optional 2000/5000-CDOM optical electrode. 15.11.1. Principle of Operation The 2000/5000-CDOM optical electrode is a submersible, fixed response fluorometer, which provides excitation at 365nm (UV) and detects any resultant fluorescence between 450nm and 520nm.
  • Page 86 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® concentrations. Fluorescence measurement techniques are not ideal for quantitative measurement. In order to obtain more accurate results, data obtained with the fluorometer in the field should be post-calibrated with data from standard laboratory analysis of grab samples acquired during the study.
  • Page 87 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Calibration RapidCal  DO 100% Full Cal Select Full Cal. The screen will change to: Calibration pH/REDOX(ORP)  DO/EC Aux Electrodes Select Aux Electrodes. The screen will change to: SELECT ELECTRODE l 4:EMPTY 1:CDOM ...
  • Page 88 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Output:2500mV Calibrating 100% Press [OK] The Calibration Report on the top line displays the voltage output from the electrode in millivolts (mV). This value is stored in the Probe's memory and can be recalled at any time. See section 10.7.
  • Page 89 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Now that this GS Factor has been applied to the electrode, all future measurements will be multiplied by 1.25 prior to being displayed. In this way, the electrode has been corrected for the local conditions and species of organic matter.

  • Page 90: Optional Ise Electrodes Calibration And Maintenance

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16. Optional ISE Electrodes Calibration and Maintenance During calibration of the ISE electrodes, very special care must be taken to achieve the specified temperatures. In order to help reduce the Probe's thermal mass and thereby speed up temperature stabilisation, it is advisable to carry out ISE calibration with the sleeve removed.

  • Page 91: 5000/7000-Amm Ammonium/Ammonia Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.4. 5000/7000-AMM Ammonium/Ammonia Electrode Ammonium (NH4) and Ammonia (NH3) can be measured by the AP-5000 using the optional 5000/7000-AMM ISE electrode within a pH range of 5 – 8. The Ammonium ISE electrode will suffer interference from Potassium, Sodium and Magnesium ions, which are similar in nature.

  • Page 92: Point 2

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Point 1. 1. Remove the storage cap from the pH electrode, wash the Probe in distilled water, dry the probe thoroughly then gently lower the Probe in to the warm 10ppm solution. ®...

  • Page 93: Point 3

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up.

  • Page 94: Two-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® successful, the counter will reach 100% and the calibration report screen will be displayed. If the temperature of the cool 10ppm solution is less than 10ºC cooler than the Pt-1 and Pt- 2 calibration temperatures, an OUT OF TEMP RANGE calibration error will be reported.

  • Page 95: 5000/7000-Nit Nitrate Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.5. 5000/7000-NIT Nitrate Electrode Nitrate (NO3) can be measured by the AP-5000 using the optional 5000/7000-CHL ISE electrode within a pH range of 3 – 10. The Nitrate ISE electrode will suffer interference from Chloride, Bromide, Fluoride, Sulphate, Chlorate and Perchlorate ions, which are similar in nature.

  • Page 96: Point 2

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Point 1. 1. Remove the storage cap from the pH electrode, wash the Probe in distilled water, dry the probe thoroughly then gently lower the Probe in to the warm 10ppm solution. ®...
  • Page 97 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed.

  • Page 98: Two-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.5.3. Two-point Calibration Two-point calibration should be carried out weekly. For this, 10ppm and 100ppm solutions are required. The two solutions can be at any temperature between 5ºC and 30ºC but they both must be the same temperature (within 1ºC).

  • Page 99: 5000/7000-Chl Chloride Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.6. 5000/7000-CHL Chloride Electrode Chloride (Cl) can be measured by the AP-5000 using the optional 5000/7000-CHL ISE electrode within a pH range of 2 – 11. The Chloride ISE electrode will suffer nterference from Bromide, Iodide, Cyanide and Sulphide ions, which are similar in nature.

  • Page 100: Point 2

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Point 1. 1. Remove the storage cap from the pH electrode, wash the Probe in distilled water, dry the probe thoroughly then gently lower the Probe in to the warm 10ppm solution. ®...

  • Page 101: Point 3

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed.

  • Page 102: Two-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.6.3. Two-point Calibration Two-point calibration should be carried out weekly. For this, 10ppm and 100ppm solutions are required. The two solutions can be at any temperature between 5ºC and 30ºC but they both must be the same temperature (within 1ºC).

  • Page 103: 5000/7000-Cal Calcium Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.7. 5000/7000-CAL Calcium Electrode Calcium (Ca2) can be measured by the AP-5000 using the optional 5000/7000-CHL ISE electrode within a pH range of 4 – 9. The Calcium ISE electrode will suffer interference from Magnesium, Barium, Lead, Zinc and Sodium ions, which are similar in nature.

  • Page 104: Point 2

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Point 1. 1. Remove the storage cap from the pH electrode, wash the Probe in distilled water, dry the probe thoroughly then gently lower the Probe in to the warm 10ppm solution. ®...

  • Page 105: Point 3

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up. If the calibration is successful, the counter will reach 100% and the following screen will be displayed.

  • Page 106: Two-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.7.3. Two-point Calibration Two-point calibration should be carried out weekly. For this, 10ppm and 100ppm solutions are required. The two solutions can be at any temperature between 5ºC and 30ºC but they both must be the same temperature (within 1ºC).

  • Page 107: 5000/7000-Flu Fluoride Electrode

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 16.8. 5000/7000-FLU Fluoride Electrode Fluoride (F) can be measured by the AP-5000 using the optional 5000/7000-CHL ISE electrode within a pH range of 4 – 8. The Fluoride ISE electrode will suffer interference from hydroxide (OH-) ions, which are similar in nature.

  • Page 108: Three-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Once all three solutions are at a stable temperature, calibration can begin. 16.8.2. Three-point Calibration During three-point calibration, the AP-5000 and Aquameter ® must remain switched on. If the Aquameter ® is switched off between points, the calibration process will be aborted and must be re-started from point 1.

  • Page 109: Point 3

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The dates shown to the right of each point are the dates of the last successful calibration. 8. Select Pt-1. The screen will change to: PLEASE WAIT Stabilising 000% The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place.

  • Page 110: Two-Point Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The Meter will wait until the readings are stable, then it will send the calibration command to the Probe, where the calibration takes place. During calibration, the Calibrating screen is displayed and the progress counter counts up as shown above. If the calibration is successful, the counter will reach 100% and the calibration report screen will be displayed.

  • Page 111: Aqualink Pc Software

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 17. AquaLink PC Software AquaLink is a utility program designed to run under Microsoft ® Windows ® on a stand-alone PC with a minimum screen resolution of 1024 x 768 and an available USB 2.0 socket. 17.1.

  • Page 112: Uploading Data From Your Aquameter

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Select your preferred operating language by clicking on one of the national flags. ® 17.5. Uploading Data From Your Aquameter ® Ensure your Aquameter has batteries installed but is switched off. Connect the Aquameter ®...

  • Page 113: O Nscreen Help

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® can be displayed as Degrees and decimal Minutes (DD MM.MMMM) or as decimal Degrees (DD.DDDDD). Select one format or the other by clicking one of the two options at the bottom of the GPS box. Positional accuracy of lat/lon co-ordinates is +/- 10 meters with a 3D Position fix.
  • Page 114 Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® and position, the total number of readings, an analysis of the highest and lowest readings, the variance between the highest and lowest readings, the average readings and the GLP data. Each block of individual readings, laid out in chronological order, follows this page. This report can be imported into any text editor or word processor package.

  • Page 115: Typical Text Report Cover Page

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 17.12. Typical Text Report Cover Page AquaLink REPORT ---------------------------- File name: C:\Test\3 day test 024690136.txt Operator name: G.E.M. Company name: Aquaread ® Site name: Test Site 4 Start date and time: 24-Jul-2009 10:09:33 Start position: Lat: N 51°21.4989' Lon: E 001°24.3232' OSGB: TR 370 677 End date and time:...

  • Page 116: Exporting Excel Files

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 17.13. Exporting Excel ® Files To export an Excel ® file, click on the ‘Export as Excel File’ button. You will be asked to specify a file name. A .xls extension will automatically be added. Excel ®...

  • Page 117: Google™ Example

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 17.16. Google™ Example Zooming in on the satellite photos in Google™ Earth is a great way to spot potential sources of pollution. If one of the readings you have taken shows an abnormality, the chances are that you will be able to spot the possible source of the problem (a riverside factory for example) directly on the satellite photo.

  • Page 118: Limited Warranty

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 18. Limited Warranty All Aquaread ® Meters are guaranteed for three years, Probes, Flow-Through Cells and individual optical electrodes are guaranteed for two years from date of purchase against defects in workmanship and materials when used for their intended purpose and maintained according to instructions.

  • Page 119: Decontamination Certificate

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 18.2. Decontamination Certificate Please print this certificate, complete all sections, and enclose it with any returned equipment. Decontamination Certificate Company Name: ____________________________________ Address: _______________________________________________________________________________ _______________________________________________________________________________ _______________________________________________________________________________ _______________________________________________________________________________ Postal code: ___________________________________ Country: ______________________________________ Phone: ________________________________________ email: __________________________________________ Product: ______________________________________ Serial No.: ______________________________________ Contaminant (if known): ___________________________________________________________________________...

  • Page 120: Troubleshooting

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 19. TROUBLESHOOTING This section details some of the common difficulties you may encounter when using the Aquameter ® , Aquaprobe ® and AquaLink software. Try all the suggested remedies. If your problem is still unresolved, contact our Service Department (service@aquaread.com). Problem Cause / Remedy The Aquameter...

  • Page 121: Declaration Of Conformity

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® Troubleshooting Continued … Problem Cause / Remedy pH and/or ORP readings  The electrodes may need re-calibrating. Recalibrate. are slow, inaccurate or  The electrodes may need cleaning. Clean as described in the unstable or calibration is relevant section of this manual.

  • Page 122: Appendix 1. The Tech Behind Aquaread's Optical Do Measurement System

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 21. Appendix 1. The Tech Behind Aquaread’s Optical DO Measurement System 21.1. Principle of Operation The Aquaread ® AquaPlus™ Optical DO measurement system works on the principle of Dynamic Luminescence Quenching. A gas-permeable chemical known as a luminophore is excited with short bursts of blue light, which causes molecules in the luminophore to emit red photons.

  • Page 123: Sensor Cap Life

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 21.2. Sensor Cap Life All optical dissolved oxygen sensors work on the same principle, and all must have the sensor cap containing the luminophore replaced periodically due to a phenomenon known as photo bleaching. When a sensor cap is new, the luminophore will return a large number of red photons when excited.

  • Page 124: Appendix 2. Installing Aux Electrodes

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 22. Appendix 2. Installing AUX Electrodes There are two types of AUX Electrodes designed for use with the AP-5000: Optical Electrodes and ISE Electrodes. These and can be fitted to any of the AUX sockets. The four AUX sockets on the AP-5000 should be populated in numeric order starting with AUX1.

  • Page 125: Socket Assignment And Calibration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 22.2. Socket Assignment and Calibration After installation, it is essential to connect the AP-5000 to an Aquameter ® and assign the new electrode types to the relevant AUX Sockets. On the Aquameter ®...

  • Page 126: Appendix 3. Standard Electrodes Detailed Specification

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 23. Appendix 3. Standard Electrodes Detailed Specification Range 0 – 500.0% / 0 – 50.00 mg/L Optical Dissolved Resolution 0.1% / 0.01mg/L Oxygen Accuracy 0 - 200%: ± 1% of reading. 200% - 500%: ± 10% Range 0 –...

  • Page 127: Appendix 4. Optical Electrodes Detailed Specification And Faqs

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 24. Appendix 4. Optical Electrodes Detailed Specification and FAQs 24.1. What are the excitation and detection wavelengths? Each Aquaread ® Optical Electrode (with the exception of Turbidity) is effectively a stand- alone, fixed frequency fluorometer, specially tuned to excite and detect fluorescence of selected substances in water.

  • Page 128: Ican See Algae In The Water But My Sensor Is Giving Low Readings . Why

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 24.3. I can see algae in the water but my sensor is giving low readings. Why? Aquaread ® Chlorophyll and Blue-Green Algae sensors are not designed to measure floating macroscopic (visible to the naked eye) algae or plant material. The sensors measure the fluorescence from the microscopic phytoplankton suspended within the body of the water below the surface.

  • Page 129: What Is The Accuracy Of The Optical Electrodes

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 24.5. What is the Accuracy of the Optical Electrodes? All Optical Electrodes, with the exception of the Turbidity Electrode, employ fluorescent measurement techniques. Interference from microbiological species and compounds which fluoresce at similar wavelengths and differences in fluorescence caused by temperature, ambient light and turbidity can all cause inaccuracies.

  • Page 130: Appendix 4. Ise Electrodes Detailed Specification

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 25. Appendix 4. ISE Electrodes Detailed Specification Range 0 – 9,000mg/L (ppm) Resolution 2 Auto-range scales: 0.00 - 99.99 mg/L, 100.0 – 8,999.9 mg/L Ammonium / Accuracy ± 10% of reading or 2ppm (whichever is greater) †...

  • Page 131: Special Notes Concerning Ise Electrodes And Ph Buffers

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® During long term deployment of ion selective electrodes, the user should obtain grab samples during the course of the deployment for analysis in the laboratory by chemical means and use the results to apply post calibration to the recorded results. Accuracy in the field is dependent upon full three-point calibration and minimal time between calibration and use.

  • Page 132: Appendix 7. Troubleshooting Turbidity

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 26. Appendix 7. Troubleshooting Turbidity The Aquaread ® Turbidity electrode is incredibly sensitive and is capable of measuring between 0 and 3000NTU with an internal resolution of greater than 0.1NTU. This means that the electrode is able to detect changes in turbidity that are less than 0.003% of the full range.

  • Page 133: About Turbidity Measurement

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 26.2. About Turbidity Measurement Turbidity is a measurement of the light scattering properties of solids suspended within a liquid and is therefore an indirect measurement of clarity. Turbidity is not a direct measurement of suspended solids, clarity or colour.

  • Page 134: Aeration

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® If you still experience negative turbidity readings and you are certain that your zero calibration solution is completely clear water, the problem is almost certainly either aeration, reflection or sensor saturation. 26.5. Aeration Aeration is air in the form of both visible and microscopic bubbles.

  • Page 135: Top Tips For Successful Measurements Using Optical Electrodes

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® The turbidity sensor's transmitter emits very short pulses of light in the infra-red spectrum at a wavelength of 850nm that are invisible to the human eye. The sensor's receiver includes a visible light filter, which filters out all visible light and allows the infra-red pulses from the transmitter to enter freely.

  • Page 136: References

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 26.9. References This section is based upon Aquaread's experience in the field and information from the following sources. ➢ National Field Manual For the Collection of Water-Quality Data, Turbidity section 6.7, Revised by Chauncey W. Anderson, USGS, 2004. ➢...

  • Page 137: Appendix 8. Flow Through Cell

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 27. Appendix 8. Flow Through Cell The AP-5000 Flow Through Cell (Flowcell) is designed for use with most third party pumping devices. The Flowcell allows sample water to flow up through the AP-5000, passing over all the individual electrodes simultaneously.

  • Page 138: Ap-5000 Installation Into The Flowcell

    Aquaprobe AP-5000 Instruction Manual 10401-00906 Rev L ® 27.3. AP-5000 Installation into the Flowcell Grease the threaded section and O ring of the Adapter Ring and screw the assembly into the Flowcell. 27.4. Flowcell Operation Connect the Flowcell to a pumping device so that sample water enters at the bottom and exits at the top.

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