Swift M10DB-MP Use And Care Manual page 7

with NA 0.65 has a maximum resolving power of 650X, equal to 1000
times the NA. This rule of NA x 1000 is true of all achromatic objectives.
WORKING DISTANCE – the distance from the lens of the objective to the
cover slip on the slide, when the specimen is in focus.
USING THE SWIFT M10D SERIES MICROSCOPE
Once you have learned the terminology and purpose of each component
of the microscope, use of the microscope is simple. By following these
steps, you will be able to begin studying the specimen quickly and easily.
1. Open the slide holder of the mechanical stage and carefully place the
slide against the fixed side and back edge of the mechanical stage.
Now slowly release the slide holder lever to hold the slide in place.
2. Align the specimen under the objective lens by using the adjustment
knobs under the mechanical stage. The bottom knob moves the slide
from right/left while the top knob adjusts the slide from front/back.
These knobs allow for precise movement and scanning of the slide.
3. Rotate the nosepiece to place the lowest power objective (4XD) over
the specimen. Be sure the objective "clicks" into position.
4. Adjust the interpupillary distance of the Siedentopf binocular head for
a comfortable view. Align the eyepiece tubes of the binocular head to
create one perfect circle, by moving the eyepiece tubes in an arc
motion.
5. While viewing through the eyepiece, rotate the coarse focus knob to
bring the specimen into focus. This should be done slowly and
carefully.
6. To adjust the contrast of the specimen, open the iris diaphragm to its
largest aperture. If additional contrast is required to permit accurate
viewing of the specimen, the diaphragm should be slowly closed until
the details of the specimen are sharply defined. Be careful not to
close the aperture too much. Although you may be achieving a higher
contrast, the fine structure of the image maybe destroyed. Reducing
the aperture increases the contrast and depth of focus, but it also
reduces resolution and introduces diffraction. The aperture must be
adjusted for each objective.
NA 0.25 for 10XD
NA 0.65 for 40XRD
NA 1.25 for 100XRD
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