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Thermo Finnigan SpectraSYSTEM UV6000LP Reference Manual

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Finnigan
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SpectraSYSTEM
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UV6000LP Detector
Reference Manual
A0099-595 Revision F

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Summary of Contents for Thermo Finnigan SpectraSYSTEM UV6000LP

  • Page 1 Finnigan ™ SpectraSYSTEM ™ UV6000LP Detector Reference Manual A0099-595 Revision F...
  • Page 2 This publication is not part of the Agreement of Sale between Thermo Electron Corporation and the purchaser of an LC system. In the event of any conflict between the provisions of this document and those contained in Thermo Electron Corporation’s Terms and Conditions, the provisions of the Terms and Conditions...
  • Page 3 Customer Registration... Register now and receive all the privileges associated with being a Thermo Electron, Finnigan product user, including application reports and technical reports. Name _______________________________________________________________________________________ Title ________________________________________________________________________________________ Company ___________________________________________________________________________________ Address _____________________________________________________________________________________ City/State/Postal Code _________________________________________________________________________ Country _____________________________________________________________________________________ Telephone _____________________________________________Ext. __________________________________ SpectraSYSTEM UV6000LP Detector Serial # ______________Date Purchased ________________________ Tell us more...
  • Page 4 From _______________________________ Place Stamp ____________________________________ Here ____________________________________ EDITOR, TECHNICAL PUBLICATIONS THERMO ELECTRON SAN JOSE 355 RIVER OAKS PARKWAY SAN JOSE, CA 95134-1991 UNITED STATES OF AMERICA fold...

  • Page 5: Table Of Contents

    Introduction ..............................3-1 LC System Setup ............................3-1 Configuring the System ..........................3-2 Quick Start with ChromQuest........................3-2 Instrument Setup............................ 3-2 Custom report ............................3-5 Preview ..............................3-6 Run Sample ............................3-7 Expected Results............................ 3-8 View Report............................3-8 January 2004 Thermo Electron...
  • Page 6 The Atlas Instrument Manager ........................5-23 Accessing the UV6000 Instrument Status Window ................5-24 Status ..............................5-24 The Operation Page..........................5-25 The Maintenance Page .........................5-27 The Error Log Page ..........................5-30 Performing a Wavelength Calibration....................5-30 Performing an Array Calibration from Atlas..................5-32 Thermo Electron January 2004...
  • Page 7 Crash Error Messages..........................6-7 Technical Assistance with Error Log Messages ..................6-8 Accessories and Replacement Parts ......................... 7-1 Field Service & Repair Procedures ........................8-1 Introduction ..............................8-1 Tools Required.............................. 8-1 Removing and Replacing UV6000LP Detector Assemblies................. 8-4 Index January 2004 Thermo Electron...

  • Page 9: Customer Support

    North America, in Europe, and in Australasia and Asia. IN NORTH AMERICA In North America, Thermo Electron Customer Service Engineers are available from each of the Thermo Electron field offices as follows: Northeastern Region Phone [1] (732) 627-0220...
  • Page 10 IN EUROPE In Europe, customer support, replaceable parts, and technical support are available from each of the Thermo Electron offices as follows: , Austria Wien (Vienna) Phone [43] (01) 333 50 34-0 [43] (01) 333 50 34-26 Brussels, Belgium Phone [32] (02) 482 30 30 [32] (02) 482 30 31 Les Ulis Cédex, France...
  • Page 11 [81] (06) 453-9110 Osaka, Japan Phone [81] (06) 6387-6681 [81] (06) 6387-6641 Beijing, P.R. China Phone [86] (010) 6621 0839 [86] (010) 6621 0851 For all other countries, contact your local Thermo Electron San Jose products dealer. January 2004 Thermo Electron...

  • Page 13: Safety And Emc Information

    (ground). When using an extension cord, make sure that the cord also has an earth contact. 4. Do not change the external or internal grounding connections. Tampering with or disconnecting these connections could endanger you and/or damage the system. January 2004 Thermo Electron...
  • Page 14 Although the large volume of toxic and flammable solvents used and stored in laboratories can be quite dangerous, don’t ignore the potential hazards posed by your samples. Take special care to read and follow all precautions that ensure proper ventilation, storage, Thermo Electron January 2004...
  • Page 15 Choose a mobile phase that’s compatible with the sample and column you’ve selected for your separation. Remember that some solvents are corrosive to stainless steel. Inert, biocompatible versions of instruments are also available from Thermo Electron. Degas the Eluents Degas your eluent solvents using either the vacuum degassing or the helium sparging technique.
  • Page 17 à correspondre à la tension locale du secteur. 2. N’utiliser que des fusibles du type et du courant nominal spécifiés. Ne pas utiliser de fusibles réparés et ne pas court- circuiter le porte-fusible. January 2004 Thermo Electron xiii...
  • Page 18 échantillon type et d’un mélange standard, bien documenté et accompagné des conditions du système. Une comparaison précise des temps de rétention, des formes et de la sensibilité des pics ainsi que des bruits Thermo Electron January 2004...
  • Page 19 Noter que certains solvants sont corrosifs pour l’acier inoxydable. Des versions inertes et biocompatibles des instruments sont disponibles auprès de Thermo Electron. Dégazage des éluants Effectuer le dégazage des éluants selon la méthode de dégazage par le vide ou à...
  • Page 20 Enfin, il est recommandé à l’opérateur de se protéger en permanence les yeux et la peau lorsqu’il travaille sur un système CL. De plus, il doit toujours mettre le système hors tension et le ramener à la pression atmosphérique avant de procéder à tout entretien. Thermo Electron January 2004...
  • Page 21 Gerät einschalten, überprüfen Sie in jedem Fall, ob die Nennspannung und die Sicherungen der Netzspannung der örtlichen Stromversorgung entsprechen. 2. Verwenden Sie nur Sicherungen des angegebenen Typs und der angegebenen Amperezahl. Verwenden Sie keine reparierten Sicherungen, und überbrücken Sie die Sicherung nicht. January 2004 Thermo Electron xvii...
  • Page 22 Ihnen, ordnungsgemäße Aufzeichnungen sämtlicher Gerätezustände (z.B. % RSDs zu Retentionszeiten, Kurvenflächen, Kurvenformen und Auflösung). Archivieren Sie als Minimum ein Chromatogramm einer typischen Probe und einer Standardmixtur mit umfassender Dokumentation der Systembedingungen zum späteren Vergleich. Ein xviii Thermo Electron January 2004...
  • Page 23 Separation verwendete Säule kompatibel ist. Dabei ist darauf zu achten, daß Edelstahl durch bestimmte Lösungsmittel korrodiert wird. Reaktionsträge, biokompatible Geräteausführungen werden ebenfalls von Thermo Electron Instruments angeboten. Entgasen des Lösungsmittels Lösungs- und Extraktionsmittel sollten entgast werden, und zwar entweder durch Vakuum oder Heliumdurchperlung. Eine umfassende Beschreibung dieser Techniken finden Sie in dem separaten Handbuch, das dem Entgasungszubehör beiliegt.
  • Page 24 Jedoch sollten auftretende Undichtigkeiten umgehend beseitigt werden. Schließlich ist noch zu empfehlen, bei der Arbeit mit einem Flüssigchromatographen stets Augen und Haut zu schützen und vor allen Wartungsarbeiten darauf zu achten, daß das Gerät abgeschaltet und druckfrei gemacht wurde. Thermo Electron January 2004...

  • Page 25: General Information

    General Information Introduction This chapter provides general information about this manual and your Thermo Electron, Finnigan UV6000LP Detector. Scope of this Manual This manual contains the information needed to install, calibrate, maintain, and troubleshoot the UV6000LP Detector. Detailed field service and repair procedures for factory trained technicians are also covered in Chapter 8 of this manual.

  • Page 26: Uv6000Lp Functional Description

    Chapter 7, Accessories and Replacement Parts - Lists accessories and user spare parts for the detector and includes Thermo Electron part numbers. Chapter 8, Field Service Procedures - provides detailed instructions for factory trained service personnel to perform field repairs.
  • Page 27 611 nm, (190 to 800 nm), the effective spacing of the diodes is 611 nm/512 = 1.2 nm. Firmware resident in the detectors’ CPU PCBA automatically interpolates diode intervals to arrive at integer wavelengths. January 2004 Thermo Electron...
  • Page 28 Transition PCBA which functions as a rear panel. A dual, 20-bit, digital-to-analog converter on the CPU PCBA provides two, single- wavelength analog outputs on the detector’s rear connectors. The detector also contains a low-voltage power supply and a lamp power supply. Thermo Electron January 2004...

  • Page 29: Installation

    2 signal cables, RJ45 cable, RJ45 9-pin adapter; and this manual. Carefully check to make sure you received all the items listed on the packing list. If any items are missing, contact your Thermo Electron representative immediately. January 2004...

  • Page 30: Installation Checklist

    Installation Checklist This is a brief summary of the steps that must be completed for the proper installation of your Thermo Electron Finnigan UV6000LP Detector. Unpack and inspect your instrument (pg. 2-1) Read the Safety section (pg. vii) Position the detector appropriately (pg. 2-3) Gather the necessary tools (pg.

  • Page 31: Making Initial Preparations

    You will need the following tools for installation: • a narrow-tip screwdriver (2 mm wide) • a #2 Phillips screwdriver • a ¼ ″ open end wrench January 2004 Thermo Electron...

  • Page 32: Checking The Power Setting And Fuses

    1. Place the tip of the narrow-blade screwdriver in the small slot to the left of the fuse holder in the power entry module and push to the right. The fuse holder should pop out. 2. Pull the fuse holder out of the power entry module. Thermo Electron January 2004...

  • Page 33: Making Initial Rear Panel Connections

    1 ch 2 1 - 1000 mv - 4 START ZERO SPARE 2 - 100 mv - 5 RS232 3 - 10 mv INPUT 3 4 5 Model: UV6000LP Serial No. Figure 2.2 Detector rear-panel connections January 2004 Thermo Electron...

  • Page 34: Computer Connection

    These outputs can also be set for 10 mV, 100 mV, or 1000 mV per AU using the switches located on the rear panel and shown in Figure 2.3. Thermo Electron January 2004...
  • Page 35 To change a range, move the appropriate switches using a small screwdriver or the tip of a pen. Remember, for each output channel, set one switch up (ON) and the other two switches down (OFF). January 2004 Thermo Electron...

  • Page 36: Remote Communications Connections

    PC-based software. During installation, you must hardwire this input to your autosampler or injector output. For Thermo Electron autosamplers, connect this input to the autosampler’s Inject Out (pin 6) output, and the UV6000LP COM terminal to the autosampler ground (pin 1).

  • Page 37: Connecting And Installing The Flowcell

    1. Remove the front cover of the detector by pulling out at the bottom edge and lifting up. 2. Remove the flowcell cover by gently pulling the snap latch to free the cover. Remove the retaining block knob and retaining block (Figure 2.5). January 2004 Thermo Electron...
  • Page 38 UV6000LP detector closest to the column outlet, as its flowcell can withstand the greatest back-pressure. A back pressure regulator is often recommended to prevent bubble formation in the detector flow cell. 2-10 Thermo Electron January 2004...

  • Page 39: Powering On For The First Time

    (rather than a solid) green light, the firmware must be downloaded. Refer to Reloading the Firmware in Chapter 4. Call your Thermo Electron service representative if you require assistance. Connecting the Remote Communications Outputs The UV6000LP has three remote outputs; RUN, READY, and EVENT (see Figure 2.2).

  • Page 40: Setting The Output Polarities From Chromquest

    Detector tab appears in the bank of tabs. 4. Click on the Detector tab to display the Instrument Status page for the UV6000 detector. 5. Change the polarities of the outputs to match the connecting equipment, if necessary. 2-12 Thermo Electron January 2004...

  • Page 41: Calibrating The Uv6000Lp

    Operation Verification Once the detector has been successfully installed, refer to Operation Verification in Chapter 4 to verify that the detector is working properly and to establish initial emission intensity spectra of the lamps. January 2004 Thermo Electron 2-13...

  • Page 42: Specifications

    Deuterium and tungsten-halogen lamps, pre-aligned *According to ASTM E1657-94 “ Standard Practice for Testing Variable -Wavelength Photometric Detectors Used in Liquid Chromatography” (for 50-mm flowcell, 5-nm bandwidth, 2-sec. rise time, and MeOH at 1 mL/min.). 2-14 Thermo Electron January 2004...
  • Page 43 15 kg Power Requirements: 100/120/220/240 Vac; 50 to 60 Hz, 200 VA max. Operating Temperature: +10 to +40 ºC Storage Temperature: - 40 °C to +70 ºC Operating Humidity: 5 to 95 % relative, non-condensing January 2004 Thermo Electron 2-15...

  • Page 45: Uv6000Lp Quick Start

    LC System Setup In order to perform this quick start procedure, you will need one ampule of the Thermo Electron LC Test Mix, 75% methanol and 25% water as the mobile phase, and a Hypersil C8 column. If required, you can substitute other samples, solvents, and columns that are convenient for you.

  • Page 46: Configuring The System

    Quick Start with ChromQuest The following section describes the proper setup of the chromatographic method for running the Thermo Electron LC Test Mix with the Microsoft Windows NT/2000/XP-based ChromQuest software. Click on the Windows taskbar Start button and move the cursor up the menu to Programs.
  • Page 47 1 nm Step 1 nm Discrete Channels Three Wavelength A 254 nm Bandwidth A 7 nm Wavelength B 430 nm Bandwidth B 7 nm Wavelength C 220 nm Bandwidth C 7 nm Scan Rate 10 Hz January 2004 Thermo Electron...
  • Page 48 To decrease short-term noise, the bandwidth setting for the discrete channels can be set higher. Because the scan and discrete channel bandwidth filters are independent, the bandwidth of the discrete channels can be set appropriately to decrease baseline noise Thermo Electron January 2004...

  • Page 49: Custom Report

    To simplify the process, Thermo Electron has included four of the most common report formats as report templates. Additional reporting capabilities are described in the ChromQuest Software User’s Guide.

  • Page 50: Preview

    Mixed View screen as a contour plot, a real-time spectrum display, a wavelength-selectable chromatogram and the peak purity index window. The Mixed View screen is easily adjusted to emphasize any of the four displays. Thermo Electron January 2004...

  • Page 51: Run Sample

    UV6000LP. (Because the UV6000LP plugs directly into the PC, it does not receive a start command from the SN4000 controller and must be “hard-wired” to the AS3000 autosampler.) At the end of the run the reports that were selected in January 2004 Thermo Electron...

  • Page 52: Expected Results

    LC Test Mix analysis is printed in the Area % Custom Report format. The report can be viewed by selecting Custom Report from the Method pull-down menu. The Method Custom Report window opens with your LC Test Mix analysis data. Thermo Electron January 2004...

  • Page 53: Routine Maintenance

    Routine Maintenance Introduction UV6000LP detectors are finely-tuned scientific instruments that we at Thermo Electron are proud to stand behind. Even so, routine maintenance is necessary to ensure optimal performance of your equipment. Procedures described in this chapter must only be performed by personnel trained in the service and maintenance of this instrument.

  • Page 54: Tools Required

    For other flowcell problems, such as a cracked window or leaks that occur in locations other than at the inlet/outlet fittings, contact your Thermo Electron representative. NOTE: Flowcells are factory-assembled units that should not be disassembled under any circumstance.

  • Page 55: Cleaning The Flowcell

    (such as when the quality of your mobile phase solvent components and the cleanliness of your samples vary). Signs of a contaminated flowcell are increased baseline noise, signal spiking, erratic or drifting baselines, low light intensity and increased back-pressure. January 2004 Thermo Electron...
  • Page 56 If you encounter contamination problems that cannot be remedied by using this cleaning procedure, contact your local Thermo Electron representative to arrange for repair or replacement. 1. Make certain that the cleaning solvent(s) you plan to use is/are miscible with the solvent already present in the flowcell and pump.

  • Page 57: Installing The Flowcell

    5. Connect the power cord to the rear panel of the detector. Wavelength and Dark Current Calibration Use the Wavelength Calibration and Dark Current Calibration procedures outlined in Chapter 5. January 2004 Thermo Electron...

  • Page 58: Error Log Printout

    Diagnostics from the pop-up menu. The Lamps page should appear. 4. Check each lamps’ status and usage. 5. If they are not already on, turn on both deuterium (D ) and tungsten (W) lamps. Thermo Electron January 2004...
  • Page 59 This scan can be kept to compare with future scans to see if there is any intensity degradation. Date the printout and add it to your maintenance records. January 2004 Thermo Electron...
  • Page 60 This scan should also be kept for future comparisons to see if there is any intensity degradation. Date the printout and add it to your maintenance records. Thermo Electron January 2004...
  • Page 61 18. Repeat the above steps to display the emission intensity spectrum of the tungsten - halogen (W) lamp. See Figure 4.4. Set the individual channels to monitor pixels 219, 302, and 427. Figure 4.4 Intensity scan with both lamps January 2004 Thermo Electron...

  • Page 62: Operation Verification Using Atlas

    Chapter 5. Use the wavelength calibration file appropriate for your normal operation. 5. Turn the tungsten - halogen (W) lamp off. This will allow you to view the intensity spectrum of the deuterium (D ) lamp alone. 4-10 Thermo Electron January 2004...
  • Page 63 7. Return to the Maintenance page and turn the tungsten - halogen (W) lamp on. 8. Return to the Display page and set the individual channels to monitor diodes 94,177, and 260. Then, click on the Start button. January 2004 Thermo Electron 4-11...
  • Page 64 11. Return to the Maintenance page and turn the deuterium (D lamp on again. Exit the Instrument Manager application and allow sufficient warm-up time before acquiring data. Figure 4.6 UV6000 Instrument Status – Display page (Atlas 2003, R1) 4-12 Thermo Electron January 2004...

  • Page 65: Replacing The Lamps

    (Figure 4.8). The screws are spring loaded and will be retained when loosened. 5. Slide the chassis out of the detector case until the metal lamp compartment cover is accessible from the right side of the instrument (Figure 4.9). January 2004 Thermo Electron 4-13...
  • Page 66 Enclosure retaining screws (recessed) Figure 4.8 Detector with Front Panel Removed Internal chassis attenuators (some models) Front chassis attenuators (some models) Detector case Chassis Lamp compartment cover Lamp cover Figure 4.9 Accessing Lamp Assembly 4-14 Thermo Electron January 2004...
  • Page 67 Figure 4.10 Removing the Lamp Cover NOTE: Do not try to remove the lamps from their mounting assemblies. Remove and replace a lamp, its mount, cable, and connector as an assembly as described in the following steps. January 2004 Thermo Electron 4-15...

  • Page 68: Adjusting Attenuators

    As lamps age their light intensities will decrease slightly. If a significant drop in light intensity occurs, the attenuators can be opened up to compensate and increase the light intensity. 4-16 Thermo Electron January 2004...

  • Page 69: Accessing The Attenuators

    8. Monitor the light intensity as you adjust the attenuators: • For Atlas, go to “Monitoring the Light Intensity from Atlas”. • For ChromQuest, go to “Monitoring the Light Intensity from ChromQuest”. January 2004 Thermo Electron 4-17...

  • Page 70: Monitoring The Light Intensity From Atlas

    10. Tighten the attenuator locking screw, replace the lamp compartment cover and foam insulation and slide the chassis back into the detector housing. 4-18 Thermo Electron January 2004...

  • Page 71: Monitoring The Light Intensity From Chromquest

    When changing mobile phases, flow solvent through the cell and readjust light levels accordingly. 10. Tighten the attenuator locking screw, replace the lamp compartment cover and foam insulation and slide the chassis back into the detector housing. January 2004 Thermo Electron 4-19...

  • Page 72: Reloading The Firmware

    • If you have not already set up a connection for the UV6000 detector, go to step 2. • If you have already set up a connection for the UV6000 detector, go to step 3. 4-20 Thermo Electron January 2004...
  • Page 73 115,200 data rate. 6. When the transfer is complete, save the UV6000LP session and close HyperTerminal. Power OFF the UV6000LP and power it back ON. January 2004 Thermo Electron 4-21...

  • Page 74: Atlas And The Exe960.Exe Utility

    Figure 4.11 Changing the directory (D: = CD ROM drive label) 8. Enter the following command: Exe960 –baud 1152000 –port com<COM_Number> -noexec pda.cof Where: <COM_Number> is the number of the COM port to which the UV6000 is connected 4-22 Thermo Electron January 2004...

  • Page 75: Operation Re-Verification

    9. After the file is downloaded, turn the UV6000LP detector off. Reconnect it for normal use. Then, turn it back on. Operation Re-verification Once you have completed diagnostics or maintenance procedures, repeat the Operation Verification procedures on page 4-6. January 2004 Thermo Electron 4-23...

  • Page 77: Diagnostics

    Most of the above actions are described in Chapter 4, Routine Maintenance. ACCESSING THE DIAGNOSTICS PROGRAM To access the Diagnostics program, do the following: 1. From the Windows taskbar, select Start, Programs (or All Programs in Windows XP), Chromatography, ChromQuest. January 2004 Thermo Electron...

  • Page 78: The Lamps Page

    The Lamps page displays the status of the detector’s two lamps and the total run time of each lamp. Buttons permit you to manually turn either lamp on or off and to reset each lamp’s run time to zero following lamp replacement (Figure 5.2). Thermo Electron January 2004...
  • Page 79 NOTE: The intensity of the deuterium lamp falls off very slightly over a period of time after it is turned on. You should wait at least 1 ½ for the lamp to stabilize after a cold start before collecting data in the UV range. January 2004 Thermo Electron...

  • Page 80: The Control Page

    A rise time filter is used in the time axis • Spectral scans and chromatogram plots are automatically zeroed at the start of data collection • Wavelength units are nanometers (190 to 800 nm) • The Y axis is in milli-absorbance units (mAU) Thermo Electron January 2004...
  • Page 81 To change a displayed value, place the text cursor in the field and use the backspace or delete key and number keys. The acceptable values are 0, 0.1, 0.2, 0.5, 1.0, 2.0, 5.0, and 10.0 seconds where 0 means no filtering. January 2004 Thermo Electron...
  • Page 82 The range of acceptable values is from 190 to 800 nm or from 2 to 511 pixels. The default is 200 nm in the absorbance mode and pixel 2 for the intensity mode. Thermo Electron January 2004...
  • Page 83 Scans Rate; 0.5, 1, 2, 4, 5, 10, and 20 with the default at 10 Hz. Channel A/B/C Select You must select the Channel check box to collect data for that channel. You can select any combination of channels or none. January 2004 Thermo Electron...

  • Page 84: The Display Page

    Data display parameters are specified on the Control page. The four buttons located in the upper right corner of the display control when and how the information is updated. They are grouped as either Status or Data. Thermo Electron January 2004...
  • Page 85 Discrete Channels Group The A, B, and C Counts/mAU fields display the intensity or absorbance of the respective channels as selected in the Control page. These fields are continuously updated when the Status-On button is clicked. January 2004 Thermo Electron...
  • Page 86 You can use this button to bring the scan back down to the baseline. This function is automatic at the start of data collection if the absorbance method is selected on the Control page. 5-10 Thermo Electron January 2004...
  • Page 87 Counts per mAU for the intensity versus absorbance axis. The Discrete Channel display color scheme can also be adjusted from the Properties box. January 2004 Thermo Electron 5-11...

  • Page 88: Using Control And Display

    220 nm and end at 800 nm. Monitor 241, 361, and 536 nm as channels A, B, and C. These three wavelengths are used in the wavelength calibration program. Then, click on Load To Detector to send settings to the UV6000LP detector. 5-12 Thermo Electron January 2004...
  • Page 89 Display page (Figure 5.7). Notice when the filter wheel is in position #1, all three channels read approximately zero. The status indicator for the filter wheel also reads On when the wheel is in position #1. January 2004 Thermo Electron 5-13...
  • Page 90 Off because the filter wheel is no longer in position #1. After inspecting the holmium oxide spectrum, be sure to return the filter wheel to the open position (position #1). 5-14 Thermo Electron January 2004...
  • Page 91 Figure 5.8 Holmium oxide absorbance spectrum January 2004 Thermo Electron 5-15...

  • Page 92: The Error Log Page

    Cancel button lets you escape if there is a problem in retrieving the log. For a list of some common error messages that may appear in the log, refer to the Log Entries section in Chapter 6 this manual . 5-16 Thermo Electron January 2004...

  • Page 93: The Calibration Page

    HINT: Be sure to check light intensities before performing the dark current or wavelength calibration procedures to ensure that the array is not saturated. Refer to the Operation Verification section on page 4-6. Figure 5.10 The Calibration Page January 2004 Thermo Electron 5-17...

  • Page 94: Performing Adark Current Calibration

    Figure 5. 12 Dark Current Calibration Complete 6. Click on the OK button. 7. Press the Apply button to apply the calibration results to the detector. Date and time of calibration are displayed and stored in memory. 5-18 Thermo Electron January 2004...

  • Page 95: Performing Awavelength Calibration

    1. Turn on both lamps and wait 1 hour for the deuterium (D lamp to warm up. 2. Pump water at 1 mL/minute through the flowcell. 3. Place the filter wheel in position 1, Open. 4. Click the Open button in the Wavelength File group. January 2004 Thermo Electron 5-19...
  • Page 96 5. Double-click on the desired file or highlight it and press the Open button. 6. The dialog box closes and the file name and its peaks are displayed in the Wavelength File group as shown in Figure 5.15. 5-20 Thermo Electron January 2004...
  • Page 97 A background spectrum is now collected. It is used to remove the mobile phase absorbance contribution. The status box at the bottom of the page will display various pieces of information describing the calibration process. January 2004 Thermo Electron 5-21...
  • Page 98 Delta values exceed ± 1 nm, click on the “Apply” button and repeat the wavelength calibration for verification. If, after applying a new calibration, the Delta values still exceed ± 1 nm, call your Thermo Electron representative for assistance. Don’t forget to apply the new calibration results after each calibration. 5-22...

  • Page 99: The Atlas Instrument Manager

    UV6000 Instrument Status window of the Instrument Manager, you can do the following: • Check lamp status and usage • View lamp intensity or absorbance spectra • Calibrate the dark current • Perform a wavelength calibration • View and print the error log January 2004 Thermo Electron 5-23...

  • Page 100: Accessing The Uv6000 Instrument Status Window

    The possible states are: Valid or Not Valid. If the status of the wavelength calibration is Not Valid, you must either perform a new wavelength calibration or reset the calibration to its default setting. 5-24 Thermo Electron January 2004...

  • Page 101: The Operation Page

    THE OPERATION PAGE The Operation page allows you to monitor detector output when data is not being acquired, for example, when the Instrument status is Idle. See Figure 5.20. January 2004 Thermo Electron 5-25...
  • Page 102 The Spectrum Start text box allows you to specify the beginning value of the scan range. The allowable values are 190 nm to 799 nm in the absorbance mode and diode number 2 to diode number 510 in the intensity mode. 5-26 Thermo Electron January 2004...

  • Page 103: The Maintenance Page

    The Maintenance page allows you to turn the lamps on and off, perform a wavelength calibration and an array calibration, turn the Ready Outputs on or off, and turn the Event Outputs on or off. See Figure 5.21. January 2004 Thermo Electron 5-27...
  • Page 104 Event Output State Turn On Button Click on this button to turn on the Event Output state. The Polarity of the Event Output is set when the UV6000 detector is configured (using the Add Instrument Wizard). 5-28 Thermo Electron January 2004...
  • Page 105 (The Reset button allows you to zero the read-back. Click on the Reset button after you install a new tungsten lamp.) Reset Button After you replace the tungsten lamp, click on this button to reset the time clock to zero. January 2004 Thermo Electron 5-29...

  • Page 106: The Error Log Page

    Turn on both lamps and wait for one hour to allow the temperature of the detector to equilibrate. In the Wavelength Calibration group box, click on the Perform New button to start the calibration open the Select Calibration File dialog box. 5-30 Thermo Electron January 2004...
  • Page 107 In the seventh page of the wizard, the results of the calibration are shown. The results table lists how close the calibration got to each peak in the calibration peak set. Click on the Apply button to accept the calibration. January 2004 Thermo Electron 5-31...

  • Page 108: Performing An Array Calibration From Atlas

    Click on the Next button to proceed to the third page of the wizard. 5. On the third page of the wizard, click on the Apply button to accept the calibration. 6. Click on the Finish button to exit the wizard. 5-32 Thermo Electron January 2004...

  • Page 109: Troubleshooting

    Check for ground loops. d. Extremely large fluctuations d. Remove systems (e.g., ovens) that in voltage on AC line. cause voltage fluctuations, isolate the detector to a “quiet” circuit, or use UPS (Uninterruptable Power Supply). January 2004 Thermo Electron...
  • Page 110 Mobile phase contamination. b. Replace with fresh mobile phase made with high-purity solvents. c. Material bleeding from c. Clean or replace column. column. d. Leaks in system, or flowcell. d. Check all fittings for leaks. Replace flowcell. Thermo Electron January 2004...
  • Page 111 (see Chapter 1). d. Power cord not connected. d. Connect power cord. 7. Detector does not go into a. Detector not receiving a. Check connection to Run contacts on run upon injection. START signal. back panel. January 2004 Thermo Electron...

  • Page 112: Error Messages

    Warning messages indicate a problem that should not affect the chromatographic run. Possible Warning messages include; “PM: A message is improperly addressed.” “PM: Free buffers back to acceptable level. You may have to lower your data rate to continue without further errors.” Thermo Electron January 2004...
  • Page 113 “Reattach: Could not send version info to PC.” “Reattach: Could not read startup message (gp).” “Reattach: An invalid startup message was received.” “Reattach: Could not echo startup message (gp).” “Write: Could not transmit a packet header. Communications are probably lost.” January 2004 Thermo Electron...

  • Page 114: Critical Error Messages

    UV6000LP to ensure future data integrity.” “ PM: Could not transmit; Communications have been lost. You will have to reconnect .” “State: Communications have been lost, tossing packets.” “Tungsten Lamp Failure.” “Deuterium Lamp Failure.” Thermo Electron January 2004...

  • Page 115: Crash Error Messages

    “Init: Creation of Timer failed. Please exit the application and retry.” “Attach: Could not get a msg to finish the attach process. You may have to exit and reconnect. In some cases you may have to cycle power on the detector.” January 2004 Thermo Electron...

  • Page 116: Technical Assistance With Error Log Messages

    In some cases you may have to cycle power on the detector.” “Read: Could not allocate PM message buffer. The UV6000LP will be unable to start.” TECHNICAL ASSISTANCE WITH ERROR LOG MESSAGES For further information, document the log entry and contact Thermo Electron Technical Support. Thermo Electron January 2004...

  • Page 117: Accessories And Replacement Parts

    Accessories and Replacement Parts Table 7.1 lists the accessories and replacement parts that you can order from Thermo Electron. Table 7.1 Accessories and Replacement Parts Optional Accessories 803264 Filter Wheel for linearity calibration, (5 position; 1 cuvette with perchloric acid blank and 4 cuvettes with different concentrations of potassium...

  • Page 119: Field Service & Repair Procedures

    # 2 Phillips head screwdriver • # 1 Phillips head screwdriver with 10 inch shaft • narrow-tip (2 mm wide) standard screwdriver • corner-to-corner SMT EPROM chip removal tool (P/N 5401-1553) • 5/64 ″ Allen head wrench January 2004 Thermo Electron...

  • Page 120: Removing And Replacing Uv6000Lp Detector Assemblies

    PCBA • the lamp power supply input transformer • the low voltage power supply • the power entry module assembly • the LED assembly • the cooling fan assembly • various connector cables Thermo Electron January 2004...
  • Page 121 Transition Power Entry PCBA Module Low Voltage Power Supply Assy PCBA Lamp Supply Transformer Lamp Power Supply PCBA Lamp Assembly Figure 8.1 Replaceable Assemblies and Components January 2004 Thermo Electron...
  • Page 122 4. Slide the chassis out of the detector and place it on a non- conductive work surface. Enclosure Retaining Screws Power Power On (Recessed) Switch Figure 8.2 Accessing the interior of the UV6000LP Thermo Electron January 2004...
  • Page 123 JP7 to Front Panel LED JP10 to Filter Wheel Sensor JP1 to Main Power Supply PCBA JP5 to Lamp Power Supply PCBA P7 to Chassis Ground JP7 to Transition PCBA Figure 8.3 CPU PCBA Connector Layout January 2004 Thermo Electron...
  • Page 124 2. Disconnect the green and yellow ground lead from the Lamp Power Supply PCBA. 3. Disconnect all four connectors across the top edge of the PCBA. (Two connectors are located on each side of the PCBA.) Thermo Electron January 2004...
  • Page 125 1. Disconnect the connector in the cable bundle between the power entry module and the inlet side of the transformer. (This connector block has a small latch which must be pressed to disconnect the two sides of the connector). January 2004 Thermo Electron...
  • Page 126 1. Remove the four Phillips head screws holding the fan guard and fan in the back panel of the chassis. 2. Disconnect the power cable from the top of the fan. 3. Slide the fan forward slightly and lift it out of the chassis. Thermo Electron January 2004...
  • Page 127 1 2 5 2 5 0 2 5 0 In U .S .A Figure 8.5 Cooling Fan Removal Install a new fan using the preceding procedure in reverse order. Clean the fan guard before reinstalling it. January 2004 Thermo Electron...
  • Page 128 6. Loosen the two 5/64″ Allen head screws holding the Power Entry Module in the rear panel (Figure 8.7) and slide the entire assembly out through the rear of the chassis. 8-10 Thermo Electron January 2004...
  • Page 129 Figure 8.6 Power button and bracket removal January 2004 Thermo Electron 8-11...
  • Page 130 P10 to terminals 3 through 10 on the new power module. Remember to reinstall the “L’ shaped button protector under the power button bracket. Secure the mechanical cable to the mounting fixture on the underside of the detector chassis with a plastic tie wrap. 8-12 Thermo Electron January 2004...
  • Page 131 13. Secure the LED cable to the mounting fixture on the underside of the chassis with a plastic tie wrap. 14. Connect the other end of the cable to the JP7 connector on the CPU PCBA (Figure 8.3). January 2004 Thermo Electron 8-13...
  • Page 132 LED Retainer Partial Bottom View Figure 8.8 LED and cable removal Cable Assemblies Figure 8.10 identifies the replaceable cable assemblies inside the detector. Refer to Figures 8.3 and 8.4 to identify the proper PCBA connector locations. 8-14 Thermo Electron January 2004...
  • Page 133 Acq. PCBA Cable Front Panel LED and Cable Assy Grommet Lamp P.S. to Lamp Cables CPU to Lamp P .S. PCBA CAble CPU to T ransition PCBA Ribbon Cable Figure 5.9 Replaceable cable assemblies in UV6000LP detector January 2004 Thermo Electron 8-15...
  • Page 135 4-6 remote communications inputs, 2-8 Europe, customer support, vi remote outputs, 2-8, 2-11 customer support Australasia and Asia, vii Europe, vi North America, v file types, 5-11 filter wheel, 3 firmware, reloading, 4-20, 4-22 January 2004 Thermo Electron index-i...
  • Page 136 2 solvents initial scans, 2-13 disposal, xi location of, 4-13 routine filtration, xi run time, 5-2 used to clean the flowcell, 4-3 status, 5-2 spiking, 4-3 tungsten-halogen, 1-2 START input, 2-8 wavelength range, 1-2 index-ii Thermo Electron January 2004...
  • Page 137 6-1 wavelength calibration tungsten-halogen lamp, lifetime, 5-3 performing from Atlas, 5-30 performing from ChromQuest, 5-19 unpacking your detector, 2-1 UPS, uninterruptable power supply, 6-1 ZERO input, 2-8 UV6000LP installation, 2-1 troubleshooting, 6-1 January 2004 Thermo Electron index-iii...

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