Related Manuals for SPEX SamplePrep Genomax 2050
Summary of Contents for SPEX SamplePrep Genomax 2050
- Page 1 2050 Genomax® Automated Tissue and Cell Lyser for Biological Applications Operations Manual Spex® SamplePrep Page 1 of 26 1300-87072-1A, REV 0...
- Page 2 Copyright 2021 by Spex® SamplePrep LLC All rights reserved. No part of the work may be reproduced or transmitted in any form or by any means, electronic or mechanical; including photocopying and recording, except as may be expressly permitted by the 1976 Copyright Act or in writing from the publisher. Requests for permission should be addressed in writing to Spex®...
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Page 3: Table Of Contents
Contents 1.0 INTRODUCTION ............................5 2.0 SPECIFICATIONS ............................6 3.0 UNPACKING ..............................7 4.0 SETTING UP ............................... 8 4.1 Electrical Connection ..........................8 4.2 Cabinet Set Up ............................8 4.3 Installing the #2059 Clamp Base, #2051 Clamp and #2052 Clamp ........8 4.3.1 Installing the #2059 Clamp Base .................... - Page 4 7.3 Running the Genomax ........................20 7.4 Safety Recommendations ........................21 8.0 RUN HISTORY ............................21 8.1 System Settings ........................... 22 8.2 Administrative Lockout Features ....................22 8.2.1 Protocols in Admin Mode ......................22 8.3 Diagnostics............................23 8.3.1 Refresh Data Button ........................23 8.3.2 View Log Button ..........................
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Page 5: Introduction
PLEASE DO NOT OPERATE THE GENOMAX UNTIL YOU HAVE READ THESE INSTRUCTIONS AND ARE FAMILIAR WITH ITS CONTROLS AND CLAMP MECHANISM. THE GENOMAX IS INTENDED FOR USE ONLY BY QUALIFIED AND TRAINED PERSONNEL. 1.0 INTRODUCTION The 2050 Genomax® is designed to effectively disrupt cellular materials using the Spex Genomic DNA Isolation Kit for plant or animal tissue by vertically oscillating up to ninety-six 2 mL vials. -
Page 6: Specifications
2.0 SPECIFICATIONS Type of Grinder: Homogenizer, Bead Beater Display: Touchscreen Grinding Mechanism: Grinding balls of stainless steel, silica or zirconia ceramic beads. 28 in (71.1 cm) high (40 in, 102 cm with lid open) x 14 in (35.6 cm) Dimensions wide x 22.5 in (57.2 cm) deep including handle. -
Page 7: Unpacking
3.0 UNPACKING Carefully inspect the exterior of the packing box. Any visible damage should immediately be reported to the carrier. Remove all packing documents from the exterior of the box and file in your records. Remove the top of the shipping box, the foam packaging material, and the Genomax accessories (power cord, titer plates, etc.). -
Page 8: Setting Up
4.0 SETTING UP The 2050 Genomax weighs 100 pounds (45.5 kg). The lid opens from the front with the handle in the center. The AC Input module, fuse tray, and power cord receptacle are located on the rear of the Genomax cabinet, toward its bottom. The power (On/Off) button is on the front right, as shown in Figure 2. -
Page 9: Installing The #2059 Clamp Base
4.3.1 Installing the #2059 Clamp Base To install the #2059 Clamp Base, first locate the 3/16 in Allen wrench and the two bolts and lock washers supplied with the Genomax. Open the Genomax lid and place the clamp base on top of the shaft. -
Page 10: Touchscreen Display
5.0 TOUCHSCREEN DISPLAY The 2050 Genomax is programmed and operated through a series of touchscreen displays. Transitioning between screens, and all programming and operating commands, is done by touching the screen with a fingertip or stylus. Do not use a sharp point as this can damage or deface the screen. -
Page 11: Adding A User
5.2.2 Adding a User To add a new user, touch the Add button on the User List screen (Figure 5) which will bring up the Edit/Add User screen. Touch inside the box, as shown in Figure 7. Touching the Back Arrow button (top left) allows the user to return to the previous screen. -
Page 12: Control Panel
5.3 Control Panel The Control Panel displays the programmed run parameters; Date, Time and Username at the bottom right of the screen; and Protocol name at the bottom center of the screen, as shown in Figure 8. Changes to the run settings are made from this screen. To recall stored run protocols, touch the Store/Recall button located at the bottom right of the screen. -
Page 13: Stopping Or Pausing A Run
5.3.2 Stopping or Pausing a Run To stop a program, touch the Stop button on the RUN SCREEN (Figure 9), the button will dim or turn a muted red and the program will end. If the Run button is touched after the program has stopped then the entire program will start over. -
Page 14: Saved Protocols
5.5 Saved Protocols The SAVED PROTOCOLS screen is shown in Figure 11. In this example, four saved protocols have been stored and named (2401M Animal, 2401MS Animal Stbl, 2402P Plant, 2402PT Plant Tough). The four saved protocols have been preprogrammed to be used with the Spex Genomic DNA isolation kits for animal and plant tissue. -
Page 15: Editing A Protocol
Touch the SAVE button to save the label shown in the Box. The label will appear as the name of the program in the SAVED PROTOCOLS Screen (Figure 11). The CANCEL key does not change anything on the screen, except returns the display to the SAVED PROTOCOLS Screen. -
Page 16: Loading The #2051 Multi-Purpose Clamp
6.1 Loading the #2051 Multi-Purpose Clamp Note: The #2059 Clamp Base must be attached to the Genomax before placing the #2051 Multi-Purpose Clamp in the unit. (Section 4.3.1) Place the sample vials (in the appropriate holder or cryo-block) in the #2051 Clamp. The sample load must be evenly distributed at all sides of the clamp to avoid excess wear to the Genomax. -
Page 17: Loading The #2052 Titer Plate Clamp
6.2 Loading the #2052 Titer Plate Clamp Titer plates are made by many companies and are sealed in various ways. The Genomax is designed for use with titer plates. Spex #2205-50 Titer Plates and #2206-50 Caps are reinforced to withstand with rigorous bead beating and have been tested not to leak when properly sealed in the #2052 Clamp. - Page 18 4. The #2052T nesting tray set consists of four trays that have a recess on both sides for the bottom of titer plate and the top of the titer plate (cap mat). A rubber membrane is placed on top of each titer plate to provide better sealing.
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Page 19: Removing The Sample Vials, Tubes, Or Titer Plates
6.3 Removing the Sample Vials, Tubes, or Titer Plates When the Genomax comes to a complete stop, open the lid by grasping the red handle and lift upwards. 2. It is not necessary to remove the clamp mechanism entirely. The #2051 or #2052 Clamp can remain attached to the #2059 Clamp Base so that only the clamp lid is removed allowing sample vials and titer plates to be easily accessed. -
Page 20: Preparing The Titer Plates
7.1 Preparing the Titer Plates While the precise details are left to the individual user, each well in the titer plate can be loaded with a seed, plant tissue, or other sample, and an eluent, solvent, etc. If it is necessary to disrupt the sample, a grinding media is typically added first. -
Page 21: Safety Recommendations
In addition, the Genomax should only be used with accessories provided by or recommended by Spex SamplePrep and must be used in the intended manner. Use of accessories not recommended by Spex SamplePrep may negatively affect the safety protection provided by the unit. -
Page 22: System Settings
8.1 System Settings To access the Settings screen, touch the Settings Icon on the Home screen (Figure 4). The system can be upgraded, diagnostics view/exported, and files exported (Run History, Saved Protocols) and saved protocols imported, as well as the time display changed from 12- hour to 24-hour, and Time and Date can be entered or changed, as shown in Figure 18. -
Page 23: Diagnostics
8.3 Diagnostics The Diagnostics Screen can be accessed from the Settings Screen (Figure 18). Touching the Back Arrow button returns the user to the previous screen. 8.3.1 Refresh Data Button Touching the Refresh Data button allows the user to take a reading of the data displayed in Figure 19. Figure 2 8.3.2 View Log Button Touching the View Log button allows the user to view... -
Page 24: Error Messages
9.0 ERROR MESSAGES 9.1 Lid Error If the lid lock fails while the Genomax is running, the unit will stop running and the screen will display a “LID OPEN” message. The timer will maintain the time remaining in the run. To restart, close the lid and press the green RESUME button to finish the run. -
Page 25: Changing The Fuses
Genomax. 11.0 WARRANTY Spex SamplePrep guarantees its products against defects in materials or workmanship for one (1) year from the date of original shipment. Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced. -
Page 26: Product Specifications
11.2 To Arrange a Return Shipment We want you to be happy with whatever you purchase from Spex SamplePrep. Please bring any problem to our attention, but please DO NOT RETURN any item before contacting us for a Return Authorization Number and instructions.
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