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65 Liberty St., Metuchen, NJ 08840
1-855-GET-SPEX (438-7739) or 732-623-0465
www.spexsampleprep.com
1200C Chilled GenoLyte®
Patented
OPERATING MANUAL
For 1200C Chilled GenoLyte®, this manual is Part No. 87068
1300-87068-1A, REV 0

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Summary of Contents for SPEX SamplePrep 1200C Chilled GenoLyte

  • Page 1 65 Liberty St., Metuchen, NJ 08840 1-855-GET-SPEX (438-7739) or 732-623-0465 www.spexsampleprep.com 1200C Chilled GenoLyte® Patented OPERATING MANUAL For 1200C Chilled GenoLyte®, this manual is Part No. 87068 1300-87068-1A, REV 0...
  • Page 2 1976 Copyright Act or in writing from the publisher. Requests for permission should be addressed in writing to SPEX SamplePrep LLC, 65 Liberty Street, Metuchen, NJ 08840.
  • Page 3: Table Of Contents

    1200C Chilled GenoLyte® TABLE OF CONTENTS SECTION DESCRIPTION PAGE Introduction ........................4 Specifications ........................ 5 Unpacking ........................6 Setting Up ........................7 Electrical Connection ....................7 Cabinet Set-Up ......................7 Standard Safety Features ..................... 7 CE Safety Features ......................8 General Safety ......................
  • Page 4: Introduction

    1200C Chilled GenoLyte® 1.0 INTRODUCTION The 1200C Chilled GenoLyte® is an efficient, compact laboratory homogenizer that uses a circulating-chiller (not included) to maintain sample temperature between 0 to 10⁰C while effectively disrupting cellular materials by horizontally oscillating 2 mL vials. Sample materials that can be prepared include seeds, stems, roots, leaves, and animal tissue.
  • Page 5: Specifications

    1200C Chilled GenoLyte® 2.0 SPECIFICATIONS Type of Mill: Homogenizer, Bead Beater (temperature controlled by external chiller) Chiller: Minimum Temperature Run Setting: - 5⁰C (23⁰F) (Sold Separately) Maximum Temperature Run Setting: 10⁰C (50⁰F) Compatible Chiller Cole-Parmer Polystat Standard (#EW-20873-47) (Recommended) Cole-Parmer Polystat Digital (#EW-10124-00)
  • Page 6: Unpacking

    2 mL vials are necessary for processing samples but must be purchased separately. The full range of preloaded 2 mL vials for the 1200C Chilled GenoLyte® is described in our catalog, The Handbook of Sample Preparation and Handling, GenoLyte Accessories Manual or www.spexsampleprep.com.
  • Page 7: Setting Up

    1200C Chilled GenoLyte® 4.0 SETTING UP The 1200C Chilled GenoLyte® weighs 24 pounds (10.9 Kg). The lid opens from the front with the handle in the center. The chiller quick-connectors are located on left and right of unit (Figure 2). The AC Inlet and On/Off switch are located on the back of the cabinet, as shown in Figure 1.
  • Page 8: Ce Safety Features

    (e.g. 3000 rpm) and sample hardness. Ear protection is recommended. 5.0 OPERATION NOTE: Never run the 1200C Chilled GenoLyte Homogenizer with the clamp arm unfastened or not properly secured. Damage and leakage will result. 5.1 Control Panel 5.1.1 Run Parameter...
  • Page 9: Homogenization

    The samples can be ground dry or with the addition of a lysis buffer. Refer to the supplier extraction kit instructions. Note: Extraction kits are not included with the 1200C Chilled GenoLyte or sold by SPEX SamplePrep.
  • Page 10: Chiller Setting Reference Table 1

    1200C Chilled GenoLyte® 6.1 Temperature Controlled Homogenization (Cont’d) 6.2 Chiller Setting Reference Table 1 (Guidelines for 1200C Chilled GenoLyte Run Protocol) CHILLER RUNTIME RATE REST CYCLE VIAL TEMPERATURE SETTING (during run) -3°C (26.6°F) 40 seconds 3000 rpm 15 seconds 0° to 4°C -1°C (30.2°F)
  • Page 11: Pre-Loaded Disruption Tubes Reference Table 3

    1200C Chilled GenoLyte® 6.3 Pre-Loaded Disruption Tubes Reference Table 3 PART NUMBER 2 ML VIAL SET DETAILS Economical bead for disrupting 2301-100MB 100 μm Silica Beads (1200 mg) bacteria. Suitable for small tissue samples 2302-1400AW 1.4 mm Zirconia Beads and biomass.
  • Page 12: Chiller Connection

    1200C Chilled GenoLyte® 7.0 CHILLER CONNECTION Chiller Connection Assembly Male Coupling ¼ Hose (to chiller) Quick Release Button Quick Release Button Figure 7 - Chiller Connection (left side view) 7.1 Description The chiller connection assembly is attached to the 1200C, fitted with two female valved coupling body for the inlet and outlet chiller ports, as shown in Figure 7.
  • Page 13: Error Messages

    8.0 ERROR MESSAGES 8.1 Lid Error If the lid interlock fails while the 1200C Chilled GenoLyte is running, the unit will stop running and the screen will display a “LID OPEN” message. The timer will maintain the time remaining in the run. To restart, close the lid and press the START button to finish the run.
  • Page 14: Warranty

    Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced. Items not produced by SPEX SamplePrep LLC carry the manufacturer’s warranty only.
  • Page 15: Instrument Disposal

    1200C Chilled GenoLyte® 11.0 INSTRUMENT DISPOSAL In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic Equipment, all equipment with the disposal symbol must not be disposed of with general waste. (See Figure 8) Disposal Label is located on the back of unit.
  • Page 16: Contact Us

    Fax: 732-906-2492 Website: www.spexsampleprep.com E-mail: sampleprep@spex.com Outside the United States and Europe, contact the SPEX SamplePrep representative from whom you purchased your equipment. A list of our current representatives is on our website, www.spexsampleprep.com. SPEX® SamplePrep LLC Page 16 of 16...

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