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WATERS CORPORATION TQ Operator's Manual

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Waters TQ Detector IVD
Operator's Guide
71500126802IVD Revision C
Copyright © Waters Corporation 2006–2012
All rights reserved

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  • Page 1 Waters TQ Detector IVD Operator’s Guide 71500126802IVD Revision C Copyright © Waters Corporation 2006–2012 All rights reserved...

  • Page 2: Copyright Notice

    Copyright notice © 2006–2012 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. Trademarks ACQUITY UPLC, Connections INSIGHT, and Waters are registered trademarks of Waters Corporation.

  • Page 3: Contacting Waters

    From the USA or Canada, phone 800 252-HPLC, or fax 508 872-1990. For other locations worldwide, phone and fax numbers appear in the Waters Web site. Conventional mail Waters Corporation 34 Maple Street Milford, MA 01757 December 6, 2012, 71500126802IVD Rev. C...

  • Page 4: Safety Considerations

    Always follow Good Laboratory Practice, and consult your organization’s safety representative for guidance. Considerations specific to the TQ Detector IVD Solvent leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate.
  • Page 5 High temperature hazard To avoid burn injuries, avoid touching the source enclosure Warning: with your hand when operating or servicing the instrument. TQ Detector high temperature hazard Source enclosure assembly PO W ER O PER ATE DESOLVATION DESOLVATION NEBULIZER NEBULIZER...

  • Page 6: Canada Spectrum Management Emissions Notice

    High voltage hazard Warning: • To avoid electric shock, do not remove the TQ Detector’s protective panels. The components they cover are not user-serviceable. • To avoid non-lethal electric shock, any equipment connected to the ESI probe must be grounded.

  • Page 7: Equipment Misuse Notice

    Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. December 6, 2012, 71500126802IVD Rev. C...

  • Page 8: Operating This Instrument

    For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. For in vitro diagnostic use viii...

  • Page 9: Intended Use

    The Waters TQ Detector IVD is CE-marked according to the European Union In Vitro Diagnostic Device Directive 98/79/EC. The Waters TQ Detector IVD can be used for general in vitro diagnostic applications. However, only professionally trained and qualified laboratory personnel can use the instrument for those purposes.

  • Page 10: Quality Control

    Quality control Routinely run three quality-control samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that quality-control sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when quality-control samples are out of range might not be valid.

  • Page 11: Ism Classification

    ISM classification ISM Classification: ISM Group 1 Class A This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical, (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment.

  • Page 12: Ec Authorized Representative

    EC authorized representative Waters Corporation (Micromass UK Ltd.) Floats Road Wythenshawe Manchester M23 9LZ United Kingdom Telephone: +44-161-946-2400 Fax: +44-161-946-2480 Contact: Quality manager December 6, 2012, 71500126802IVD Rev. C...

  • Page 13: Table Of Contents

    Copyright notice ....................ii Trademarks ......................ii Contacting Waters ....................iii Safety considerations ..................iv Considerations specific to the TQ Detector IVD ..........iv FCC radiation emissions notice ................. vi Canada spectrum management emissions notice ..........vi Electrical power safety notice ................vi Safety hazard symbol notice................
  • Page 14 Ion optics ......................25 MS operating modes ..................26 MS/MS operating modes ................... 27 Product (daughter) ion mode................27 Precursor (parent) ion mode................28 Multiple reaction monitoring mode ..............28 Constant neutral loss mode................29 Sample inlet ......................30 Leak sensors ......................30 Vacuum system ....................
  • Page 15 3 ESI Operation ..................47 Introduction ......................48 Installing the ESI probe ................... 48 Removing the ESI probe .................. 52 4 Maintenance Procedures ..............53 Maintenance schedule ..................55 Spare parts ......................56 Troubleshooting with Connections INSIGHT ..........56 Safety and handling ..................57 Preparing the instrument for work performed on the source ....
  • Page 16 Cleaning the ion block, isolation valve, and extraction cone ....84 Removing the ion block assembly from the source assembly ......84 Disassembling the source ion block assembly..........86 Cleaning the ion block, isolation valve, and extraction cone......94 Assembling the source ion block assembly............96 Fitting the ion block assembly to the source assembly........
  • Page 17 Replacing the roughing pump oil ..............135 Replacing the roughing pump’s oil demister element ......138 A Safety Advisories .................. 145 Warning symbols ....................146 Task-specific hazard warnings................ 146 Specific warnings ..................... 147 Caution symbol ....................149 Warnings that apply to all Waters instruments ........150 Electrical and handling symbols ..............
  • Page 18 Connecting to the electricity source ............187 C Materials of Construction and Compliant Solvents ..... 189 Preventing contamination ................190 Items exposed to solvent ................190 Solvents used to prepare mobile phases ............ 191 xviii December 6, 2012, 71500126802IVD Rev. C...

  • Page 19: Waters Tq Detector

    Waters TQ Detector This chapter describes the instrument, including its controls and gas and plumbing connections. Contents Topic Page Overview................... 20 Ionization techniques and source probes ........24 Ion optics ..................25 MS operating modes ................ 26 MS/MS operating modes..............27 Sample inlet ..................

  • Page 20: Overview

    (API) mass spectrometer. Designed for routine UPLC™/MS/MS analyses in quantitative and qualitative applications, it can operate at fast acquisition speeds compatible with ultra-performance liquid-chromatography (UPLC). For instrument specifications, see the Waters TQ Detector Site Preparation Guide. Waters TQ Detector TP02592...
  • Page 21 Overview Waters TQ Detector, with doors open PO W ER O PER ATE DESOLVATION DESOLVATION NEBULIZER NEBULIZER APPI APPI PROBE PROBE IntelliStart technology IntelliStart™ technology monitors LC/MS/MS performance and reports when the instrument is ready for use. The software automatically tunes and mass calibrates the instrument and displays performance readbacks.

  • Page 22: Acquity Tqd Uplc/Ms System

    ACQUITY TQD UPLC/MS system The ACQUITY TQD UPLC/MS system includes an ACQUITY UPLC system and the Waters TQ Detector. If you are not using your instrument as part of an ACQUITY UPLC system, refer to the documentation for your LC system.
  • Page 23 Overview the system and its modules. The ACQUITY UPLC Console functions independently of the MassLynx software and does not recognize or control the data systems. December 6, 2012, 71500126802IVD Rev. C...

  • Page 24: Ionization Techniques And Source Probes

    1 Waters TQ Detector Ionization techniques and source probes Electrospray ionization (ESI) In electrospray ionization (ESI), a strong electrical charge is given the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation). As solvent continues to evaporate, the charge density increases until the droplet surfaces eject ions (ion evaporation).

  • Page 25: Ion Optics

    Ion optics Ion optics The instrument’s ion optics operate as follows: Samples from the LC or Intellistart fluidics system are introduced at atmospheric pressure into the ionization source. The ions pass through the sample cone into the vacuum system. The ions pass through the transfer optics, to the first quadrupole where they are filtered according to their mass-to-charge ratios.

  • Page 26: Ms Operating Modes

    2000 Da/s. It also provides a useful tool for instrument tuning and calibration before MS/MS analysis and for fault diagnosis. See the instrument online Help for further information on optimizing the TQ Detector for fast MS scanning.

  • Page 27: Ms/Ms Operating Modes

    MS/MS operating modes MS/MS operating modes The following table shows the MS/MS operating modes. MS/MS operating modes Operating mode Collision cell Product Static (at Pass all masses Scanning (daughter) ion precursor mass) spectrum Precursor Scanning Static (at product (parent) ion mass) spectrum Static (at...

  • Page 28: Precursor (Parent) Ion Mode

    1 Waters TQ Detector – Identifying product ions for use in MRM transitions. – Optimizing CID tuning conditions to maximize the yield of a specific product ion to be used in MRM analysis. Precursor (parent) ion mode Precursor ion mode...

  • Page 29: Constant Neutral Loss Mode

    MS/MS operating modes Multiple reaction monitoring mode Collision cell Static (at precursor mass) Pass all masses Static (at product mass) Typical application You typically use MRM to quantify known analytes in complex samples: • Drug metabolite and pharmacokinetic studies. • Forensic or toxicology, for example, screening for target drugs in sport.

  • Page 30: Sample Inlet

    Leak sensors Where fitted, leak sensors in the drip trays of the ACQUITY UPLC system and the TQ Detector continuously monitor system components for leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 mL of accumulated, leaked liquid in its surrounding reservoir.

  • Page 31: Rear Panel

    Rear panel A vacuum isolation valve isolates the source from the mass analyzer, allowing routine source maintenance without venting. Rear panel The following figure shows the rear panel locations of the connectors used to operate the instrument with external devices. Instrument rear panel Anal og O ut...

  • Page 32: Intellistart Fluidics System

    1 Waters TQ Detector IntelliStart Fluidics system Overview The IntelliStart Fluidics system is built into the instrument. The system delivers sample directly to the MS probe in one of two ways: • From the LC column. • From two integral reservoirs.

  • Page 33: System Operation

    IntelliStart Fluidics system System operation Control of solvent and sample delivery during auto-tuning, auto-calibration, and method development is automatically performed by the software. IntelliStart Fluidics configuration requirements can be set in the system console. You can edit the parameters, frequency, and extent of the automation you want IntelliStart to perform.
  • Page 34 1 Waters TQ Detector December 6, 2012, 71500126802IVD Rev. C...

  • Page 35: Preparing For Operation

    Preparing for Operation This chapter describes how to start and shut-down the instrument. Contents Topic Page Starting the instrument ..............36 Preparing the IntelliStart Fluidics system........40 Rebooting the instrument..............45 Leaving the mass spectrometer ready for operation...... 46 December 6, 2012, 71500126802IVD Rev. C...

  • Page 36: Starting The Instrument

    ACQUITY™. Starting the instrument entails powering-on the ACQUITY workstation, logging into the workstation, powering-on the TQ Detector and all the other ACQUITY instruments and devices, and starting the MassLynx software. You must power-on and log in to the ACQUITY workstation Requirement: first to ensure that it obtains the IP addresses of the system instruments.
  • Page 37 • After the instruments are successfully powered-on, all power LEDs show steady green. The binary solvent manager’s flow LED, the sample manager’s run LED, and the TQ Detector’s Operate LED remain off. Start the MassLynx software. Click IntelliStart in the MassLynx main window’s lower left-hand corner to launch IntelliStart.

  • Page 38: Configuring Intellistart

    Click Configure > IntelliStart Configuration. In the IntelliStart Configuration dialog box, in the Checks list, select the check boxes for the items you want checked during TQ Detector startup. Clear the check boxes of items you do not want checked.

  • Page 39: Monitoring The Instrument Leds

    Starting the instrument and desolvation temperatures. IntelliStart automatically sets these parameters when you enter a flow rate, according to the following table. Flow rate versus temperature and gas flow Flow rate Desolvation Desolvation gas Source temp (°C) (mL/min) temp (°C) flow (L/h) 0.000 to 0.100 0.101 to 0.300...

  • Page 40: Preparing The Intellistart Fluidics System

    2 Preparing for Operation Preparing the IntelliStart Fluidics system For additional information, see page 179. Installing the solvent manifold drip tray Required material Chemical-resistant, powder-free gloves To install the solvent manifold drip tray The solvent manifold drip tray can be contaminated Warning: with biohazardous and/or toxic materials.

  • Page 41: Installing The Reservoir Bottles

    Preparing the IntelliStart Fluidics system Installing the reservoir bottles Use standard reservoir bottles (15-mL) for instrument setup and calibration. The Low-volume Adaptor Kit is included for infusing smaller volumes. The capacity of the low-volume vials is 1.5 mL. Use standard reservoir bottles (15-mL) for instrument setup and calibration. Required material Chemical-resistant, powder-free gloves To install the reservoir bottles...

  • Page 42: Diverter Valve Positions

    2 Preparing for Operation Screw the low-volume adaptor into the manifold and tighten it finger-tight. Screw the low-volume vial into the adaptor. TP02630 Diverter valve positions Column and syringe in home position after power-up After power-up, the flow path between the column and waste is open. The syringe is empty, and the flow path between it and waste is open.
  • Page 43 Preparing the IntelliStart Fluidics system LC position In the LC position, the flow path between the LC and probe is open, and the flow path between the syringe and waste is also open. Probe Idle Column Syringe Waste Reservoir A Reservoir B Infusion position Probe...
  • Page 44 2 Preparing for Operation Combined position with LC flow and syringe in idle mode Probe Combined Idle Column Syringe Waste Reservoir A Reservoir B Waste position In the waste position, both the LC flow and the infusion syringe flow are directed to waste.

  • Page 45: Purging The Infusion Syringe

    Rebooting the instrument Purging the infusion syringe Whenever you replace a solution bottle, purge the infusion syringe with the solution that you are going to use next. See the mass spectrometer’s online Help for details. Depending on the solutions used, the IntelliStart Fluidics system can Tip: require more than one purge cycle to minimize carryover.

  • Page 46: Leaving The Mass Spectrometer Ready For Operation

    Help for details. Emergency instrument shutdown To shut down the instrument in an emergency The TQ Detector’s power switch does not isolate the Warning: instrument from the main power supply. To isolate the instrument, you must disconnect the power cable from the back of the instrument.

  • Page 47: Esi Operation

    ESI Operation This chapter describes how to prepare the instrument for ESI (electrospray ionization). Contents Topic Page Introduction..................48 Installing the ESI probe ..............48 Removing the ESI probe ..............52 December 6, 2012, 71500126802IVD Rev. C...

  • Page 48: Introduction

    3 ESI Operation Introduction The ESI ionization mode options use the standard ESI probe that is fitted to the instrument when it is shipped from the factory. The following sections explain how to install and remove the ESI probe. For further instruction, see page Installing the ESI probe On instruments bearing the probe compatibility...
  • Page 49 Installing the ESI probe To install the ESI probe The ACQUITY UPLC system connections, ESI probe, Warning: and source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. To avoid electric shock, ensure that the instrument is Warning: suitably prepared before commencing this procedure.
  • Page 50 3 ESI Operation Ensure that the contacts on the ESI probe align with the probe adjuster assembly contacts, and carefully slide the ESI probe into the hole in the probe adjuster assembly. ESI probe Probe adjuster assembly Probe adjuster assembly contacts TP02632 Secure the ESI probe by tightening the 2 thumbscrews.
  • Page 51 Installing the ESI probe ESI probe mounted on the source enclosure, showing the connections Vernier probe adjuster Thumbscrew Nebulizer gas connection Diverter valve POWER OPERATE Desolvation gas NEBULIZER DESOLVATION connection APPI ESI probe electrical lead PROBE Probe adjuster assembly electrical lead Probe adjuster assembly ESI probe Connect the ESI probe’s PTFE tube to the nebulizer gas connection.

  • Page 52: Removing The Esi Probe

    3 ESI Operation Removing the ESI probe Chemical-resistant, powder-free gloves Required material: To remove the ESI probe The ACQUITY UPLC system connections, ESI probe, Warning: and source can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.

  • Page 53: Maintenance Procedures

    Maintenance Procedures This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents Topic Page Maintenance schedule ..............55 Spare parts ..................56 Troubleshooting with Connections INSIGHT ........

  • Page 54: Contents

    4 Maintenance Procedures Contents Topic Page Maintaining the instrument air filters ........... 131 Replacing the roughing pump oil ............ 135 Replacing the roughing pump’s oil demister element....138 December 6, 2012, 71500126802IVD Rev. C...

  • Page 55: Maintenance Schedule

    Maintenance schedule Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance. The maintenance frequencies shown apply to instruments that normally receive moderate use. Maintenance schedule Procedure Frequency For information... Clean the instrument case. As required. page Empty the exhaust trap bottle Check daily, empty as...

  • Page 56: Spare Parts

    4 Maintenance Procedures Maintenance schedule Procedure Frequency For information... Clean or replace the Annually. page 131. instrument’s air filters. Change the roughing pump Annually. page 135. oil. Replace the roughing pump’s Annually. page 138. demister element. Applications that Tip: contaminate the roughing pump oil reduce this period, which must be...

  • Page 57: Safety And Handling

    Safety and handling Consult these sources for more information about Connections INSIGHT and Connections INSIGHT iAssist: • http://www.waters.com. • Connections INSIGHT Installation Guide (part number 715001399) • Connections INSIGHT User's Guide (part number 715001400) • Your sales representative. • Your local Waters subsidiary. •...
  • Page 58 4 Maintenance Procedures To avoid electric shock, Warning: • do not remove the instrument’s panels. There are no user-serviceable items inside the instrument. • ensure that the instrument is in Standby mode before commencing any maintenance. The probe and source can be hot. To avoid burn injuries, take Warning: great care while working with these components.

  • Page 59: Preparing The Instrument For Work Performed On The Source

    In the Instrument Console, click Stop Flow to stop the LC flow or, if column flow is required, divert the LC flow to waste as follows: In the Instrument Console system tree, expand TQ Detector, Interactive Fluidics. Click Control Select Waste as the flow state.

  • Page 60: Operating The Source Isolation Valve

    4 Maintenance Procedures Operating the source isolation valve You must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. Chemical-resistant, powder-free gloves Required material: To close the source isolation valve before starting a maintenance procedure The source components can be contaminated with Warning: biohazardous and/or toxic materials.
  • Page 61 Operating the source isolation valve Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Isolation valve handle in closed position To open the source isolation valve after completing a maintenance procedure The source components can be contaminated with Warning: biohazardous and/or toxic materials.

  • Page 62: Removing O-Rings And Seals

    4 Maintenance Procedures Removing O-rings and seals When performing certain maintenance procedures, you must remove O-rings or seals from instrument components. An O-ring removal kit is provided with the instrument. You must dispose of all used O-rings and seals; do not re-use old O-rings or seals on the instrument.

  • Page 63: Cleaning The Instrument's Exterior

    Cleaning the instrument’s exterior Cleaning the instrument’s exterior Do not use abrasives or solvents to clean the instrument’s Caution: case. Use a soft cloth, dampened with water, to clean the outside surfaces of the instrument. Emptying the exhaust trap bottle Check the exhaust trap bottle in the instrument exhaust line daily and empty it before it is completely full.
  • Page 64 4 Maintenance Procedures To empty the exhaust trap bottle In the ACQUITY UPLC Console, click Stop Flow to stop the LC flow. In the ACQUITY UPLC Console, click API to stop the desolvation gas flow. The waste liquid in the exhaust trap bottle Warning: comprises ACQUITY UPLC solvents and analytes.

  • Page 65: Emptying The Roughing Pump Exhaust Liquid Trap Bottle

    Emptying the roughing pump exhaust liquid trap bottle Use snoop (or equivalent) leak detector liquid to ensure that there are no leaks at the exhaust trap bottle cap. Emptying the roughing pump exhaust liquid trap bottle Check the liquid trap bottle in the roughing pump exhaust line daily and empty it before it is completely full.
  • Page 66 4 Maintenance Procedures Roughing pump exhaust liquid trap bottle for an oil-free roughing pump TP02997 TP02800 TP02996 Required materials: • Chemical-resistant, powder-free gloves • snoop (or equivalent) leak detector liquid To empty the exhaust liquid trap bottle Close the source isolation valve (see page 60).
  • Page 67 Emptying the roughing pump exhaust liquid trap bottle Unscrew and remove the roughing pump liquid trap bottle cap. The waste liquid can be contaminated with Warning: biohazardous and/or toxic materials. Ensure that it is correctly disposed of according to local environmental regulations.

  • Page 68: Gas Ballasting The Roughing Pump

    4 Maintenance Procedures Gas ballasting the roughing pump This procedure is not required for an Alcatel oil-free roughing pump. Note: Roughing pump Exhaust port flange Oil filler plug Oil-level sight glass TP02689 Drain plug Gas ballast valve Failure to routinely gas ballast the roughing pump shortens Caution: oil life and, consequently, pump life.

  • Page 69: Gas Ballasting A Pump Fitted With A Screwdriver-Operated Gas Ballast Valve

    Gas ballasting the roughing pump • A handle-operated gas ballast valve. See page Gas ballasting a pump fitted with a screwdriver-operated gas ballast valve Flat-blade screwdriver Required material: To gas ballast the roughing pump To avoid burn injuries, take great care while working with Warning: the roughing pump: it can be hot.

  • Page 70: Gas Ballasting A Pump Fitted With A Handle-Operated Gas Ballast Valve

    4 Maintenance Procedures Use the flat-blade screwdriver to turn the gas ballast valve to the closed, , position. Gas ballasting a pump fitted with a handle-operated gas ballast valve To gas ballast the roughing pump To avoid burn injuries, take great care while working with Warning: the roughing pump: it can be hot.

  • Page 71: Checking The Roughing Pump Oil Level

    Checking the roughing pump oil level Move the gas ballast valve handle on the pump clockwise from the vertical position to the horizontal position. Checking the roughing pump oil level To ensure correct operation of the roughing pump, do not Caution: operate the pump with the oil level at less than 30% of the MAX level.
  • Page 72 4 Maintenance Procedures To add oil to the roughing pump Vent and shut-down the instrument (see the mass spectrometer’s online Help for details). The pump oil can be contaminated with analyte Warning: accumulated during normal operation. Always wear chemical-resistant, powder-free gloves when adding or replacing oil.

  • Page 73: Cleaning The Source Components

    Cleaning the source components • After running the pump for 12 to 48 hours, it is common to see a few drops of oil near the filler plug. Excess oil around the lip of the filler plug will run down and drip off the pump once the pump reaches operating temperature.

  • Page 74: Cleaning The Sample Cone And Gas Cone

    4 Maintenance Procedures Cleaning the sample cone and gas cone The cone gas assembly (comprising the sample cone, O-ring, and gas cone) can be removed for cleaning without venting the instrument. Removing the cone gas assembly from the source Chemical-resistant, powder-free gloves Required material: To remove the cone gas assembly from the source The source components can be contaminated with...
  • Page 75 Cleaning the sample cone and gas cone Disconnect the PTFE tube from the cone gas assembly connection tube. Cone gas assembly Connection tube PTFE tube Grasp the cone gas assembly connection tube, and use it as a lever to rotate the cone gas assembly 90 degrees, moving the connection tube from the vertical to the horizontal position.
  • Page 76 4 Maintenance Procedures Do not open the isolation valve at any time when the Caution: cone gas assembly has been removed from the ion block assembly. Slide the cone gas assembly out of the ion block assembly. Ion block assembly December 6, 2012, 71500126802IVD Rev.

  • Page 77: Disassembling The Cone Gas Assembly

    Cleaning the sample cone and gas cone Disassembling the cone gas assembly Chemical-resistant, powder-free gloves Required material: To disassemble the cone gas assembly The cone gas assembly can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.

  • Page 78: Cleaning The Sample Cone And Gas Cone

    4 Maintenance Procedures Carefully push down on the gas cone to separate the gas cone, sample cone, and O-ring. O-ring Sample cone Gas cone RP00021 The O-ring can be contaminated with Warning: biohazardous and/or toxic materials. Ensure that it is correctly disposed of according to local environmental regulations.
  • Page 79 Cleaning the sample cone and gas cone • Wash-bottle containing HPLC-grade, or better, 1:1 methanol/water. • Large beaker. To clean the sample cone and gas cone The sample cone and gas cone can be contaminated Warning: with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 80 4 Maintenance Procedures Inspect each component for persisting contamination. If contamination is present, do as follows: Use the wash-bottle containing 1:1 methanol/water to rinse the component over the large beaker. Blow-dry the component with inert, oil-free gas. Inspect each component for persisting contamination. If contamination is present, dispose of the component, and obtain a new one before reassembling the cone gas assembly.

  • Page 81: Assembling The Cone Gas Assembly

    Cleaning the sample cone and gas cone Assembling the cone gas assembly Chemical-resistant, powder-free gloves Required material: To assemble the cone gas assembly Caution: • To avoid recontaminating the cone gas assembly, wear clean chemical-resistant, powder-free gloves during this procedure. •...

  • Page 82: Fitting The Cone Gas Assembly To The Source

    4 Maintenance Procedures Fitting the cone gas assembly to the source Chemical-resistant, powder-free gloves Required material: To fit the cone gas assembly to the source The source components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 83 Cleaning the sample cone and gas cone Ensure that the source isolation valve is in the closed position (see page 60). Hold the cone gas assembly so that the connection tube is horizontal and at the top, then slide the cone gas assembly into the ion block assembly. Ion block assembly Cone gas...

  • Page 84: Cleaning The Ion Block, Isolation Valve, And Extraction Cone

    4 Maintenance Procedures Cleaning the ion block, isolation valve, and extraction cone The ion block and extraction cone must be cleaned if cleaning the sample cone and gas cone fails to increase signal sensitivity. Removing the ion block assembly from the source assembly Required materials: •...
  • Page 85 Cleaning the ion block, isolation valve, and extraction cone Use the 6-mm Allen wrench to unscrew and remove the 2 ion block assembly securing screws and associated washers. Washers Ion block securing screws RP000010 Remove the ion block assembly from the PEEK ion block support. Ion block assembly RP00011 December 6, 2012, 71500126802IVD Rev.

  • Page 86: Disassembling The Source Ion Block Assembly

    4 Maintenance Procedures Disassembling the source ion block assembly Required materials: • Chemical-resistant, powder-free gloves • O-ring removal kit • Needle-nose pliers To disassemble the ion block assembly The ion block assembly can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 87 Cleaning the ion block, isolation valve, and extraction cone To ensure correct operation of the ion block assembly after Caution: reassembly, • the gas cone position block must not be removed • the screws holding the gas cone position blocks in place must not be adjusted.
  • Page 88 4 Maintenance Procedures Grasp the isolation valve and pull it out of the ion block. Isolation valve O-ring Use the O-ring removal kit to carefully remove the isolation valve O-ring (see page 62). The isolation valve O-ring can be contaminated Warning: with biohazardous and/or toxic materials.
  • Page 89 Cleaning the ion block, isolation valve, and extraction cone If the 2 set screws securing the heater cartridge to the PEEK terminal block are fitted, use the 1.5-mm Allen wrench to loosen them. TP02616 Heater cartridge securing set screws 10. Use the 2.5-mm Allen wrench to loosen the captive PEEK terminal block securing screw.
  • Page 90 4 Maintenance Procedures To avoid damaging the heater cartridge assembly Caution: wires, do not bend or twist them either side of the heater cartridge assembly heat-shrink tubing when removing the assembly from the ion block. 11. Use the needle-nose pliers to grasp the PEEK terminal block and partially lift it out of the ion block.
  • Page 91 Cleaning the ion block, isolation valve, and extraction cone 13. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also page 62). Cover seal TP02619 The cover seal can be contaminated with Warning: biohazardous and/or toxic materials.
  • Page 92 4 Maintenance Procedures 15. Use the 6-mm Allen wrench to remove the ion block blanking plug and associated seal. Blanking plug Seal TP02648 The blanking plug seal can be contaminated with Warning: biohazardous and/or toxic materials. Ensure that it is correctly disposed of according to local environmental regulations.
  • Page 93 Cleaning the ion block, isolation valve, and extraction cone 17. Use the 2.5-mm Allen wrench to loosen the captive extraction cone retainer securing screw. Securing screw Extraction cone TP02612 Caution: • Take great care not to damage the extraction cone aperture when removing the extraction cone from the ion block.

  • Page 94: Cleaning The Ion Block, Isolation Valve, And Extraction Cone

    4 Maintenance Procedures 19. Remove the extraction cone retainer from the extraction cone. 20. Remove the extraction cone seal from the ion block. Extraction cone seal TP02614 Cleaning the ion block, isolation valve, and extraction cone Required materials: • Chemical-resistant, powder-free gloves. •...
  • Page 95 Cleaning the ion block, isolation valve, and extraction cone To clean the ion block components The ion block components can be contaminated with Warning: biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Use extreme care when working with formic acid. Use a fume Warning: hood and appropriate protective equipment.

  • Page 96: Assembling The Source Ion Block Assembly

    4 Maintenance Procedures component over the large beaker. Blow-dry the component with inert, oil-free gas. Inspect each component for persisting contamination. If contamination is present, dispose of the component, and obtain a new one before reassembling the ion block assembly. Assembling the source ion block assembly Required materials: •...
  • Page 97 Cleaning the ion block, isolation valve, and extraction cone Use the 6-mm Allen wrench to fit and tighten the blanking plug to the ion block. To avoid damaging the heater cartridge assembly Caution: wires, do not bend or twist them either side of the heater cartridge assembly heat-shrink tubing when fitting the assembly to the ion block.
  • Page 98 4 Maintenance Procedures 15. If an ion block assembly set screw is fitted, use the 1.5 mm Allen wrench to loosen the set screw. If an ion block assembly set screw is not fitted go step Ion block assembly set screw TP02651 16.

  • Page 99: Fitting The Ion Block Assembly To The Source Assembly

    Cleaning the ion block, isolation valve, and extraction cone Fitting the ion block assembly to the source assembly Required materials: • Chemical-resistant, powder-free gloves • 6-mm Allen wrench To fit the ion block assembly To avoid puncture wounds, take great care while working Warning: with the source enclosure door open if an ESI probe is fitted (the probe tip is sharp).
  • Page 100 4 Maintenance Procedures Do not apply any downward force to the source Caution: enclosure door while the door is open. Fit the ion block assembly to the PEEK ion block support. Use the 6-mm Allen wrench to fit and then slowly and evenly tighten the 2 ion block assembly securing screws and their associated washers.

  • Page 101: Cleaning The Source Hexapole Assembly

    Cleaning the source hexapole assembly Cleaning the source hexapole assembly The source hexapole assembly must be cleaned if cleaning the ion block, isolation valve, and extraction cone fails to increase signal sensitivity. Removing the ion block assembly, ion block support, and hexapole from the source assembly Required materials: •...
  • Page 102 4 Maintenance Procedures Remove the PEEK ion block support from the adaptor housing. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see page 62). The O-rings can be contaminated with Warning: biohazardous and/or toxic materials.

  • Page 103: Cleaning The Hexapole Assembly

    Cleaning the source hexapole assembly Cleaning the hexapole assembly Required materials: • Chemical-resistant, powder-free gloves. • 500-mL measuring cylinder or appropriately sized glass vessel in which to completely immerse the hexapole when cleaning. Use only glassware not previously cleaned with surfactants. •...
  • Page 104 4 Maintenance Procedures To avoid vibration damage to the hexapole assembly, Caution: ensure that the bottom of the assembly is not in contact with the bottom of the glass vessel. Use the hook to carefully suspend the hexapole assembly into the glass vessel with the bottom of the assembly clear of the bottom of the vessel.
  • Page 105 Cleaning the source hexapole assembly Inspect the hexapole assembly for persisting contamination, If contamination is present, do as follows: Use the wash-bottle containing methanol to rinse the source hexapole assembly over the large beaker. Blow-dry the hexapole assembly with inert, oil-free gas. Use the small flat-blade screwdriver to ensure that the hexapole assembly screws are tight.

  • Page 106: Fitting The Hexapole Assembly, Peek Ion Block Support, And Ion Block Assembly To The Source Assembly

    4 Maintenance Procedures Fitting the hexapole assembly, PEEK ion block support, and ion block assembly to the source assembly Required materials: • Chemical-resistant, powder-free gloves • 3-mm Allen wrench • Lint-free cloth • HPLC-grade, or better, 1:1 methanol/water To fit the hexapole assembly and PEEK ion block support to the source To avoid recontaminating the source, wear clean, Caution: chemical-resistant, powder-free gloves during this procedure.
  • Page 107 Cleaning the source hexapole assembly Use the 3-mm Allen wrench to fit and tighten the 3 PEEK ion block support securing screws. Look through the PEEK ion block support as you tighten the Tip: securing screws. Ensure that neither hexapole spring buckles and touches a hexapole rod.

  • Page 108: Replacing The Esi Probe Tip

    4 Maintenance Procedures Replacing the ESI probe tip Replace the ESI probe tip if a blockage occurs in the internal metal sheathing through which the stainless steel capillary passes or if the probe tip threads are damaged. Required materials: • Chemical-resistant, powder-free gloves •...

  • Page 109: Replacing The Esi Probe Sample Capillary

    Replacing the ESI probe sample capillary Replacing the ESI probe sample capillary Replace the stainless steel sample capillary in the ESI probe if it becomes blocked and cannot be cleared, or if it becomes contaminated or damaged. Required materials: • Chemical-resistant, powder-free gloves •...
  • Page 110 4 Maintenance Procedures Use the screwdriver to remove the 2 probe end-cover retaining screws. 2 retaining screws Use the 1.5-mm Allen wrench to loosen the 3 set screws on the LC PEEK union. 3 set screws December 6, 2012, 71500126802IVD Rev. C...
  • Page 111 Replacing the ESI probe sample capillary Remove the end-cover. End-cover Use the 6-mm wrench to remove the probe tip. Probe tip TP03007 December 6, 2012, 71500126802IVD Rev. C...
  • Page 112 4 Maintenance Procedures Use the 7/16-inch wrench to unscrew the coupling and union from the probe. Coupling LC union Withdraw the LC union, coupling and capillary from the probe. Remove the LC union, capillary and seal from the coupling. Seal Capillary GVF16 Ferrule Coupling...
  • Page 113 Replacing the ESI probe sample capillary 11. Slide a new GVF16 ferrule onto the PTFE liner tube. 12. Fit a new seal into the groove facing the short end of the coupling. Groove Coupling 13. Slide the coupling—short end first—onto the capillary, followed by the new PTFE liner tube and ferrule.
  • Page 114 4 Maintenance Procedures 19. Check for leaks in the assembly by attaching the free end of the PEEK tubing to an LC pump and pumping 1:1 acetonitrile/water through it at 1 mL/min. 20. If leakage occurs, disassemble and remake the connection, and then repeat the leak test.
  • Page 115 Replacing the ESI probe sample capillary 25. Attach the coupling nut to the probe, and gently tighten it with the 7/16-inch wrench. Coupling LC union 26. Replace the probe tip, and then screw down until the capillary protrudes approximately 0.5 mm from the end of the tip. Capillary protrudes 0.5 mm from the end of the probe tip 27.

  • Page 116: Replacing The Ion Block Source Heater

    4 Maintenance Procedures Replacing the ion block source heater The capillary and ferrule can be contaminated Warning: with biohazardous and/or toxic materials. Ensure that they are correctly disposed of according to local environmental regulations. Replace the ion block source heater if it fails to heat when the instrument is pumped down (evacuated).
  • Page 117 Replacing the ion block source heater Use the 2.5-mm Allen wrench to loosen the 4 captive screws securing the ion block cover plate. Ion block cover plate captive securing screw Ion block cover plate TP02649 Remove the ion block cover plate. Use the 1.5-mm Allen wrench to remove the 2 screws securing the heater wires to the PEEK terminal block.
  • Page 118 4 Maintenance Procedures Use the needle-nose pliers to carefully swing the ring terminal tags out of the terminal block. Ring terminal tag TP02617 If the 2 set screws securing the heater cartridge to the PEEK terminal block are fitted, use the 1.5-mm Allen wrench to loosen them. TP02617 Heater cartridge securing set screws December 6, 2012, 71500126802IVD Rev.
  • Page 119 Replacing the ion block source heater Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly and slide the assembly out of the ion block. Heat-shrink tubing Heater cartridge assembly TP02618 Dispose of the heater cartridge assembly. To avoid damaging the heater cartridge assembly Caution: wires, do not bend or twist them either side of the heater...
  • Page 120 4 Maintenance Procedures 13. Use the 1.5-mm Allen wrench to fit and tighten the 2 screws securing the heater wires to the PEEK terminal block. 14. Fit the ion block cover plate to the ion block assembly, and then use the 2.5-mm Allen wrench to tighten the 4 captive screws securing ion block cover plate.

  • Page 121: Replacing The Source Assembly Seals

    Replacing the source assembly seals Replacing the source assembly seals To avoid possible excessive leakage of solvent vapor Warning: into the laboratory atmosphere, the seals listed below must be renewed, at intervals of no greater than 1 year, exactly as described in this section.
  • Page 122 4 Maintenance Procedures Remove the probe from the source, see page Do not apply any downward force to the source Caution: enclosure door while the door is open. Open the source enclosure door by releasing both spring-clips and lowering the door towards you. Disconnect the probe’s electrical connection at the instrument’s front panel.

  • Page 123: Disassembling The Source Enclosure And Probe Adjuster Assembly

    Replacing the source assembly seals Disassembling the source enclosure and probe adjuster assembly Required materials: • Chemical-resistant, powder-free gloves • 4-mm Allen wrench To dismantle the source enclosure and probe adjuster assembly The source components can be contaminated with Warning: biohazardous and/or toxic materials.
  • Page 124 4 Maintenance Procedures Removing the seals from the source enclosure and probe adjuster assembly Required materials: • Chemical-resistant, powder-free gloves • Flat-blade screwdriver • O-ring removal kit To remove the seals from the source enclosure and probe adjuster assembly The source components can be contaminated with Warning: biohazardous and/or toxic materials.
  • Page 125 Replacing the source assembly seals • Probe adjuster assembly flange seal Probe adjuster assembly flange seal Source enclosure housing seal Source side flange seal RP00025 Do not apply any downward force to the source Caution: enclosure door while the door is open. Open the source enclosure door by releasing both spring-clips and lowering the door towards you.
  • Page 126 4 Maintenance Procedures Use the O-ring removal kit to carefully remove the seal from the source enclosure door. Door glass retaining screw Source enclosure glass seal Source enclosure door seal RP00019 Use the screwdriver to remove the 4 screws securing the glass-retaining plate to the source enclosure door.

  • Page 127: Fitting The New Source Enclosure And Probe Adjuster Assembly Seals

    Replacing the source assembly seals Fitting the new source enclosure and probe adjuster assembly seals Required materials: • Chemical-resistant, powder-free gloves • Wash bottle containing HPLC-grade, or better, 1:1 methanol/water To fit the new source enclosure and probe seals The source components can be contaminated with Warning: biohazardous and/or toxic materials.

  • Page 128: Assembling The Probe Adjuster Assembly And Source Enclosure

    4 Maintenance Procedures • Source enclosure housing seal • Source enclosure side flange seal • Probe adjuster assembly flange seal Fit the new probe adjuster assembly probe seal to the probe adjuster assembly. Assembling the probe adjuster assembly and source enclosure Required materials: •...

  • Page 129: Fitting The Source Enclosure To The Instrument

    Replacing the source assembly seals Fitting the source enclosure to the instrument Required materials: • Chemical-resistant, powder-free gloves • 5-mm Allen wrench To fit the source enclosure to the instrument To confirm the integrity of the source exhaust system, Warning: perform the procedure exactly as described in this section.
  • Page 130 4 Maintenance Procedures Start the instrument (see page 36). To confirm the integrity of the source exhaust Warning: system, a source pressure test must be performed, as described in the Waters Micromass Source Pressure Test Unit Operator’s Guide. Perform a source pressure test, as described in the Waters Micromass Source Pressure Test Unit Operator’s Guide.

  • Page 131: Maintaining The Instrument Air Filters

    Maintaining the instrument air filters Maintaining the instrument air filters Cleaning the air filter inside the instrument’s lower bezel Required materials: • T10 TORX driver • Vacuum cleaner To clean the air filter inside the instrument’s lower bezel Use the T10 TORX driver to remove the 5 screws that secure the lower bezel in place.

  • Page 132: Replacing The Air Filter Inside The Lower Bezel

    If you cannot clean the air filter by vacuuming, replace it with a new filter. Required materials: • T10 TORX driver • TQ Detector lower bezel air filter To replace the air filter inside the lower bezel Remove the 5 screws that secure the lower bezel in place (see the figure page 131).

  • Page 133: Cleaning The Air Filter Behind The Source Probe

    Maintaining the instrument air filters Air filter inside the lower bezel Screws Air filter Frame TP02638 Screws Remove the air filter from the air filter frame and dispose of it. Align the new air filter within the air filter frame. Use the T10 TORX driver to fit and tighten the 4 screws securing the air filter and frame to the inside of the lower bezel.
  • Page 134 4 Maintenance Procedures Air filter tab Air filter tab RP00023 Use a vacuum cleaner to clean the air filter. Air filter removed from behind the source probe Air filter Frame TP02637 Reinstall the air filter. Fit the probe to the source, see page December 6, 2012, 71500126802IVD Rev.

  • Page 135: Replacing The Air Filter Behind The Source Probe

    Replacing the roughing pump oil Replacing the air filter behind the source probe To replace the air filter behind the source probe Remove the probe from the source, see page Grasp the air filter tab and remove the air filter frame by lifting it toward you (see the figure on page 134).
  • Page 136 4 Maintenance Procedures • Container to catch used oil • Funnel • 1-L container of Anderol vacuum oil, type GS 495 To replace the roughing pump oil Gas ballast the roughing pump for 1 hour to reduce the oil viscosity (see page 68).
  • Page 137 Replacing the roughing pump oil Use the 8-mm Allen wrench to remove the oil drain plug. Tip the pump toward the drain plug aperture and allow the oil to drain completely into the container. The roughing pump oil can be contaminated with Warning: biohazardous and/or toxic materials.

  • Page 138: Replacing The Roughing Pump's Oil Demister Element

    4 Maintenance Procedures 13. Ensure that the O-ring on the oil filler plug is clean and properly seated. Observe these precautions to avoid oil leakage when Caution: fitting the oil filler plug to the roughing pump: • Ensure that the plug is not cross-threaded. •...
  • Page 139 Replacing the roughing pump’s oil demister element Allow the roughing pump to cool. The pump oil can be contaminated with analyte Warning: accumulated during normal operation. Always wear chemical-resistant, powder-free gloves when replacing the oil demister element. To avoid burn injuries, take great care while working Warning: with the roughing pump: it can be hot.
  • Page 140 4 Maintenance Procedures Using both hands, carefully remove the exhaust flange and oil demister element from the roughing pump. Oil demister element TP02693 Use the 10-mm wrench to remove the nut that secures the oil demister element to the exhaust flange. Spring Securing nut TP02686...
  • Page 141 Replacing the roughing pump’s oil demister element Holding the oil demister element slightly elevated to prevent the loss of the spring, remove the exhaust flange from the oil demister element. TP02692 Remove the spring from the oil demister element. The oil demister element can be contaminated Warning: with biohazardous and/or toxic materials.
  • Page 142 4 Maintenance Procedures To fit the new oil demister element The pump oil can be contaminated with analyte Warning: accumulated during normal operation. Always wear chemical-resistant, powder-free gloves when replacing the oil demister element. Fit the spring to the new oil demister element. TP02682 Holding the oil demister element slightly elevated to prevent the loss of the spring, fit the exhaust flange to the oil demister element.
  • Page 143 Replacing the roughing pump’s oil demister element Ensure that the inscription “TOP” is at the top of the oil demister element, and, using both hands, carefully fit the oil demister element and exhaust flange to the roughing pump. The bolts securing the source exhaust flange to the Caution: roughing pump must each be sequentially and incrementally tightened until they are all fully tight.
  • Page 144 4 Maintenance Procedures December 6, 2012, 71500126802IVD Rev. C...

  • Page 145: A Safety Advisories

    Safety Advisories Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them.

  • Page 146: Warning Symbols

    A Safety Advisories Warning symbols Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

  • Page 147: Specific Warnings

    Warning symbols (Risk of personal exposure to laser radiation.) Warning: (Risk of exposure to biological agents that can pose a serious Warning: health threat.) (Risk of tipping.) Warning: (Risk of explosion.) Warning: Specific warnings The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts.
  • Page 148 A Safety Advisories Mass spectrometer flammable solvents warning This warning applies to instruments operated with flammable solvents. Where significant quantities of flammable solvents are Warning: involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 690 kPa (6.9 bar, 100 psi) during an analysis in which flammable solvents are used.

  • Page 149: Caution Symbol

    Caution symbol Biohazard warning This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans. Waters instruments and software can be used to analyze or Warning: process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials.

  • Page 150: Warnings That Apply To All Waters Instruments

    A Safety Advisories Warnings that apply to all Waters instruments When operating this device, follow standard quality control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
  • Page 151 Warnings that apply to all Waters instruments Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. •...
  • Page 152 A Safety Advisories Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 153 Warnings that apply to all Waters instruments Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

  • Page 154: Electrical And Handling Symbols

    A Safety Advisories Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse December 6, 2012, 71500126802IVD Rev.

  • Page 155: Handling Symbols

    Electrical and handling symbols Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks! December 6, 2012, 71500126802IVD Rev. C...
  • Page 156 A Safety Advisories December 6, 2012, 71500126802IVD Rev. C...

  • Page 157: B External Connections

    External Connections This appendix describes the instrument’s external connections. The instrument is heavy. To avoid injury, Waters Warning: recommends that the instrument be lifted using suitable machinery and the supplied harness. Caution: • Contact Waters Technical Service before moving the instrument.

  • Page 158: External Wiring And Vacuum Connections

    B External Connections External wiring and vacuum connections Rear panel connections appear in the figure below. Rear panel Anal og O ut I nj ect S t ar t G r ound G r ound N ot used Event St op Fl ow O ut Sw i t ch 3 O ut...

  • Page 159: Connecting The Oil-Filled Roughing Pump

    Connecting the oil-filled roughing pump Connecting the oil-filled roughing pump To connect the alternative dry roughing pump, see page 166. Note: Exhaust port flange Oil filler plug Oil-level sight glass TP02689 Drain plug Gas ballast valve Required materials: • Chemical-resistant, powder-free gloves •...
  • Page 160 B External Connections To connect the roughing pump The pump and its connections can be Warning: contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves when performing this procedure. Caution: • To ensure correct operation of the roughing pump, the pump must be installed within 1 degree of horizontal.
  • Page 161 Connecting the oil-filled roughing pump Attach the NW25 tee, included in the startup kit, to the inlet of the pump using the NW25 center ring, and then secure the connection with a clamp. 1-inch ID vacuum hose Clamps Flange NW25 tee Pump inlet TP02625 Attach the flanged end of a length of 1-inch ID vacuum hose to each open...
  • Page 162 B External Connections Connect an approximately 36.4-cm (2.5-inch) length of 12.7-mm clear PVC exhaust tubing to the roughing pump exhaust port NW25 nozzle fitting. Secure the tubing with a hose clamp. To prevent condensation from draining backward and Caution: damaging the pump, the exhaust tubing must be installed with an exhaust trap bottle;...
  • Page 163 Connecting the oil-filled roughing pump Secure the exhaust trap bottle in a conspicuous location. The instrument requires two separate exhaust systems: Caution: one for nitrogen, the other for the roughing pump. Vent them to atmosphere through separate exhaust lines. Oil mist can seriously damage the instrument if the nitrogen exhaust line connects with the roughing pump exhaust line.

  • Page 164: Making The Electrical Connections For A Roughing Pump With An External Relay Box

    B External Connections Making the electrical connections for a roughing pump with an external relay box Roughing pump connections with an external relay box Detector rear panel A nal og O ut I nj ect St ar t Roughing pump main G r ound G r ound N ot used...

  • Page 165: Making The Electrical Connections For A Roughing Pump Without An External Relay Box

    Connecting the oil-filled roughing pump Connect the relay box power connector to the main power source. Making the electrical connections for a roughing pump without an external relay box Roughing pump connections without an external relay box Detector rear panel Roughing pump d.c.

  • Page 166: Connecting The Oil-Free Roughing Pump

    B External Connections Connecting the oil-free roughing pump The oil-free roughing pump is an optional alternative to the standard oil-filled roughing pump. To connect the oil-filled roughing pump, see page 159. The noise reduction cover must be installed over the pump to minimize operating noise, unless the pump is installed in a cabinet that includes sound dampening and ventilation.
  • Page 167 Connecting the oil-free roughing pump • NW25 full nipple (included in the Alcatel pump kit) • DN25 reducing nipple (included in the Alcatel pump kit) • NW40 center rings (included in the Alcatel pump kit) • NW40 clamps (included in the Alcatel pump kit) •...
  • Page 168 B External Connections Install the noise reduction cover. DN 25 nipple DN 40 nipple Clamps TP02996 December 6, 2012, 71500126802IVD Rev. C...
  • Page 169 Connecting the oil-free roughing pump To connect the oil-free roughing pump The pump and its connections can be Warning: contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves when performing this procedure. Caution: • To ensure correct operation of the roughing pump, the pump must be installed within 1 degree of horizontal.
  • Page 170 B External Connections Attach the isolation valve, NW25 tee, and elbows to the DN40 nipple on the pump inlet, and then secure these connections with clamps, as shown in the figure below. NW25 tee Elbow 1-inch ID vacuum hose Isolation valve DN40 nipple on pump inlet TP02997...
  • Page 171 Connecting the oil-free roughing pump Attach the DN25 elbow to the DN25 nipple on the pump exhaust outlet and secure the connection with a clamp. Install the external silencer, with the arrow pointing toward the pump, to the open port of the DN25 elbow, and then secure the connection with a clamp.
  • Page 172 B External Connections To avoid gas leaks, use the sharp knife to cut the PVC Caution: exhaust tubing squarely (that is, perpendicular to its horizontal axis). 10. Connect a length of 12.7-mm clear PVC exhaust tubing to an elbow and connect the elbow to the other fitting on the exhaust trap bottle.

  • Page 173: Making The Electrical Connections For An Oil-Free Roughing Pump

    Connecting the oil-free roughing pump Making the electrical connections for an oil-free roughing pump Roughing pump connections Detector rear panel Anal og O ut I nj ect S t ar t G r ound G r ound N ot used Event St op Fl ow O ut...

  • Page 174: Connecting To The Nitrogen Gas Supply

    B External Connections Connecting to the nitrogen gas supply Required materials: • Chemical-resistant, powder-free gloves • Sharp knife • Wrench • 6-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) To connect the nitrogen gas supply To avoid gas leaks, use the sharp knife to cut the PTFE Caution: tubing squarely (that is, perpendicular to its horizontal axis).
  • Page 175 Connecting to the nitrogen gas supply Gas and exhaust connections Anal og O ut I nj ect S t ar t G r ound G r ound N ot used Event St op Fl ow O ut Sw i t ch 3 O ut G r ound G r ound...

  • Page 176: Connecting To The Collision Cell Gas Supply

    B External Connections Connecting to the collision cell gas supply Required materials: • Chemical-resistant, powder-free gloves • Wrench ® • 1/8-inch Swagelok nut and ferrule • 1/8-inch stainless steel tube (supplied with the instrument) To connect the collision cell gas supply Use the 1/8-inch Swagelok nut and ferrule to connect the 1/8-inch stainless steel tube to the collision cell gas inlet on the rear of the instrument (see the figure on...
  • Page 177 Connecting the nitrogen exhaust line To connect the nitrogen exhaust line Warning: • LC solvents and analytes can be carried in the nitrogen exhaust, which must be vented via the nitrogen exhaust trap bottle and laboratory exhaust system. The laboratory exhaust system must provide a minimum vacuum of 0.20 kPa (2 mbar, 0.03 psi) below atmospheric pressure (negative pressure).
  • Page 178 B External Connections Insert one end of the tubing into the remaining port on the exhaust trap bottle. Route the other end to the exhaust vent. To confirm the integrity of the source exhaust Warning: system, the following leak test must be performed. To avoid damage to the instrument, snoop (or its Caution: equivalent) leak detector liquid must be used only for the...

  • Page 179: Connecting The Liquid Waste Line

    Connecting the liquid waste line Connecting the liquid waste line Chemical-resistant, powder-free gloves Required material: To connect the liquid waste line The waste line and connection can be contaminated Warning: with biologically hazardous materials. Always wear chemical-resistant, powder-free gloves while performing this procedure.
  • Page 180 B External Connections To prevent leakage of biologically hazardous Warning: materials, ensure that the • drain line does not crimp or bend. A crimp or bend can impede flow to the waste container. • waste container is emptied before the lower end of the drain tube is covered by waste solvent.

  • Page 181: Connecting The Workstation

    Connecting the workstation Connecting the workstation Before connecting the workstation to the instrument, set up the workstation according to its accompanying instructions. Locate the workstation within 5 meters (16 feet) of the instrument. Shielded network cables must be used with the instrument to Requirement: ensure compliance with FCC, and other, limits.

  • Page 182: Connecting Ethernet Cables

    B External Connections Connecting Ethernet cables Shielded Ethernet cables must be used with the instrument to Requirement: ensure compliance with FCC, and other, limits. To make Ethernet connections Connect one end of one shielded Ethernet cable to the network switch, and then connect the other end to the Ethernet card on the preconfigured ACQUITY™...
  • Page 183 I/O signal connectors I/O signal connectors Connector I Connector II   Analog (Out) Inject Start (In)   Analog (Out) Inject Start (In) Ground Ground  Not Used Event (In)  Not Used Event (In)  Stop Flow (Out) Switch 3 (Out) ...

  • Page 184: Signal Connections

    B External Connections Signal connections Instrument analog-out/event-in connections Signal connections Description Analog (Out) Used for analog chart output functionality. The output voltage range is 0 to 2 V. The resolution of the voltage output is 12 bits. Stop Flow (Out) Used to stop the solvent flow if the nitrogen gas supply fails.
  • Page 185 I/O signal connectors To make the signal connections, attach the positive and negative leads of the signal cable to the connector. Connector Signal cable TP02585 Slide the clamp (with the bend facing down) into the protective shield. Insert the clamp and shield (with the bend facing down) into the connection cover, and loosely tighten with one self-tapping screw.
  • Page 186 B External Connections Insert the connector with the signal cable into the connection cover, and position the clamp over the cable leads. Tighten the clamp into place with the second self-tapping screw. Cable leads Clamp Place the second connection cover over the first cover, and snap it into place.

  • Page 187: Connecting To The Electricity Source

    Connecting to the electricity source Connecting to the electricity source The instrument requires a separate, grounded electricity source. The ground connection in the electrical outlet must be common and connected near the system. To connect to the electricity source Use a line conditioner or an uninterruptible power supply Recommendation: (UPS) for optimum long-term input voltage stability.
  • Page 188 B External Connections December 6, 2012, 71500126802IVD Rev. C...

  • Page 189: C Materials Of Construction And Compliant Solvents

    Materials of Construction and Compliant Solvents To confirm the integrity of the source exhaust Warning: system, you must address any safety issues raised by the contents of this Appendix. Contents Topic Page Preventing contamination ............... 190 Items exposed to solvent..............190 Solvents used to prepare mobile phases .........

  • Page 190: Preventing Contamination

    C Materials of Construction and Compliant Solvents Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). You can find this document on http://www.waters.com. Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents normally used with these items.

  • Page 191: Solvents Used To Prepare Mobile Phases

    Solvents used to prepare mobile phases Items exposed to solvent Item Material Source enclosure view port Toughened plate glass Trap bottle Polypropylene Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel, polybutylene terephthalate, and polyoxymethylene Solvents used to prepare mobile phases These solvents are the most common ingredients used to prepare mobile phases for reverse-phase LC/MS (API): •...
  • Page 192 C Materials of Construction and Compliant Solvents December 6, 2012, 71500126802IVD Rev. C...

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