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WATERS CORPORATION ACQUITY UPLC Operator's, Overview And Maintenance Manual

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ACQUITY UPLC
TUV Detector
Operator's Overview and Maintenance Guide
Revision A
Copyright © Waters Corporation 2010
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Summary of Contents for WATERS CORPORATION ACQUITY UPLC

  • Page 1 ACQUITY UPLC TUV Detector Operator’s Overview and Maintenance Guide Revision A Copyright © Waters Corporation 2010 All rights reserved...

  • Page 2: Copyright Notice

    Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

  • Page 3: Customer Comments

    Customer comments Waters’ Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.

  • Page 4: Contacting Waters

    252-HPLC, or fax 508 872 1990. For other locations worldwide, phone and fax numbers appear in the Waters Web site. Conventional mail Waters Corporation 34 Maple Street Milford, MA 01757 Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards.

  • Page 5: Considerations Specific To The Acquity Tuv Detector

    Considerations specific to the ACQUITY TUV detector High voltage hazard To avoid electric shock, do not remove the TUV detector’s Warning: protective panels. The components within are not user-serviceable. Safety advisories Consult the Safety Advisories section on page 62 for a comprehensive list of warning and caution advisories.

  • Page 6: Operating The Acquity Tuv Detector

    Operating the ACQUITY TUV detector When operating this instrument, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia C-Tick EMC Compliant Confirms that a manufactured product complies...

  • Page 7: Calibrating

    Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards must include the entire range of QC samples, typical specimens, and atypical specimens. When calibrating mass spectrometers, consult the calibration section of the operator’s guide for the instrument you are calibrating.

  • Page 8: Authorized Representative Information

    Authorized representative information Authorized representative Waters Corporation (Micromass UK Limited) is registered in the United Kingdom with the Medicines and Healthcare Products Regulatory Agency (MHRA) at Market Towers, 1 Nine Elms Lane, London, SW8 5NQ. Waters Corporation (Micromass UK Ltd.)

  • Page 9: Table Of Contents

    Table of Contents Copyright notice ....................ii Trademarks ......................ii Customer comments .................... iii Contacting Waters ....................iv Safety considerations ..................iv Considerations specific to the ACQUITY TUV detector ........v Safety advisories ....................v Operating the ACQUITY TUV detector ............vi Applicable symbols .....................
  • Page 10 Making Ethernet connections ................. 20 I/O signal connector ................... 21 Connecting to the electricity source ............. 21 Starting the detector ..................22 Monitoring detector LEDs ................. 24 About the detector control panel............... 25 Shutting down the detector ................27 Shutting down for less than 24 hours............... 27 Shutting down for more than 24 hours.............

  • Page 11: Overview

    Overview The Waters ACQUITY UPLC tunable ultraviolet (TUV) optical detector is a two-channel, ultraviolet/visible (tunable UV/Vis) absorbance detector designed for use in ACQUITY UPLC or ACQUITY UPLC H-Class systems. The detector, controlled by Empower, MassLynx, or third-party software for both LC/MS and LC applications, operates as an integral part of the system.

  • Page 12: Principles Of Operation

    • Thermal wander management – To mitigate thermal instability caused by ambient temperature changes, the detector’s insulation ensures air flow across the optics bench, and its variable speed fan runs at higher or lower speeds, as needed. The fan normally changes speeds in response to the thermal changes.
  • Page 13 Waters ACQUITY TUV detector optics assembly Optics assembly light path The detector provides an extremely efficient design for exceptionally high light throughput. It operates as follows: The ellipsoidal mirror collects light from the lamp and focuses it through the filter wheel onto the entrance slit. The spherical mirror directs light toward the grating.
  • Page 14 When you specify a new wavelength via Empower, MassLynx, or third-party software, the detector rotates the grating to the appropriate position. The preamplifier board integrates and digitizes the currents from the photodiodes for processing by the signal processing electronics and output to a computer, chart recorder, or integrator.
  • Page 15 In the ACQUITY UPLC TUV detector, this angle is mechanically controlled by components external to the flow cell so that the variation in refractive index arising from different mobile phases does not materially influence the efficiency of the transmitted energy.
  • Page 16 light intensity. The reduction is subsequently converted to absorbance. The light exits the flow cell through a fused silica window, where it projects onto the sample photodiode. Unlike other flow cell designs, where the light beam is designed to avoid striking the internal walls of the cell, light-guiding relies on internal reflections from the walls of the Teflon AF tubing.
  • Page 17 Higher time-constant settings produce these effects: • Greatly decrease baseline noise • Shorten and broaden peaks The software includes fast or normal filtering constants at each data rate that are appropriate for high speed or high sensitivity applications, respectively. The following figure shows the relationship between increased filter time-constant and absorbance.

  • Page 18: Wavelength Verification And Test

    Wavelength verification and test The detector’s deuterium arc lamp and integral erbium filter exhibit peaks in the transmission spectrum at known wavelengths. Upon startup, the detector verifies calibration by comparing the locations of these peaks with expected wavelengths based on calibration data stored in the detector’s memory. If the results of this verification differ from the stored calibration by more than 1.0 nm, the detector displays a Wavelength Verification Failure message.

  • Page 19: Operational Modes

    Operational modes The detector operates in single or dual-wavelength mode, allows spectrum scanning using a flow cell, and provides RatioPlot, difference plot, and MaxPlot functions. Single-wavelength mode Single-wavelength is the detector’s default mode of operation that supports monitoring a single-wavelength, from 190 nm to 700 nm, settable in 1-nm increments on channel A.
  • Page 20 rounded up or down to a value based on the data rate. Selecting Other and entering a value of 0.0 disables all filtering. • Analog rate – Specifies a value as high as 80 Hz. Dual-wavelength mode In dual-wavelength mode, the detector can monitor two wavelengths, one on channel A and one on channel B.
  • Page 21 when observing, with one data channel, multiple compounds that exhibit absorbancies at two separate wavelengths. (Dual mode only) • RatioPlot (A/B) – This mode produces the ratio of absorbance from two wavelengths. Theoretically, the ratio is constant for a pure chromatographic peak and variable for an impure peak, which results in a nonsquared response.

  • Page 22: Before You Begin

    To install the detector, you should generally know how to set up Requirement: and operate laboratory instruments and computer-controlled devices and how to handle solvents. Use this guide in conjunction with the ACQUITY UPLC system Tip: documentation and online Help. Before installing the detector, ensure that •...

  • Page 23: Installing The Detector

    For complete information on reporting shipping damages and submitting claims, see Waters Licenses, Warranties, and Support Services. Installing the detector To install the ACQUITY UPLC TUV detector When installing the detector unassisted by another Warning: person, use a mechanical lift to avoid lifting injuries.
  • Page 24 Place the solvent tray module atop the detector. ACQUITY UPLC TUV detector in an ACQUITY UPLC H-Class system Bottle tray Detector Column heater Sample manager - flow through needle Quaternary solvent manager...

  • Page 25: Plumbing The Detector

    To avoid particulate contamination in the flow cell, flush Recommendation: any columns you are connecting to the detector before connecting them. ACQUITY UPLC System Documentation CD or ACQUITY UPLC See also: H-Class System Documentation CD. Plumbing the detector...
  • Page 26 To plumb the detector If the detector is already powered on, in the console, select Recommendation: TUV Detector from the system tree and click (Lamp Off) to extinguish the lamp. Open the detector’s front door, and install the flow cell assembly, holding it squarely to the opening and then inserting it slowly so that the guides on the front part of the flow cell flange engage the rails in the sample cell compartment.
  • Page 27 Hand tighten the thumbscrews. Confirm the screws are secure using a screw driver. Flow cell ID connector Lamp Outlet tubing Thumbscrews Lamp ID Handle Flow cell assembly Leak sensor Backpressure Inlet tubing TP03261 regulator Remove the protective cover from the PEEK cell inlet tubing, and connect the tubing to the flow cell inlet, confirming that the label on the tubing matches the type of detector and flow cell in your system.
  • Page 28 Attach the short length of outlet tubing from the backpressure regulator to the outlet of the flow cell. Backpressure regulator Direction of flow from detector outlet To waste TP03260 Route the long end of the outlet tubing from the backpressure regulator, through the channel clips along the front right side of the system, and into a suitable waste container.

  • Page 29: Installing The Multi-Detector Drip Tray

    Installing the multi-detector drip tray If your ACQUITY UPLC system has more than one detector, you must install the multi-detector drip tray. ACQUITY UPLC TUV detector in a split ACQUITY UPLC H-Class system Column heater Sample manager - flow through needle...

  • Page 30: Making Ethernet Connections

    Installing the multi-detector drip tray (bottom view) Screws Extended Plastic plastic feet rivets Return the ACQUITY TUV detector to its original position atop the other detector. Making Ethernet connections To make Ethernet connections Unpack and install the preconfigured ACQUITY workstation. Connect one end of one Ethernet cable to the network switch, and then connect the other end to the Ethernet card, on the workstation.

  • Page 31: I/O Signal Connector

    Europe. For other countries, contact your local Waters distributor. • Power-off and unplug the detector before performing any maintenance on the instrument. • Connect all components of the ACQUITY UPLC system to a common ground. Connecting to the electricity source...

  • Page 32: Starting The Detector

    To connect to the electricity source Use a line conditioner and uninterruptible power supply Recommendation: (UPS) for optimum long-term input voltage stability. Connect the female end of the power cord to the receptacle on the rear panel of the detector. Connect the male end of the power cord to a suitable wall outlet.
  • Page 33 Inlet Editor window. • In the console, select TUV Detector from the system tree and click (Lamp Off). ACQUITY UPLC System Documentation CD or ACQUITY UPLC See also: H-Class System Documentation CD. To start the detector Power-on the workstation.

  • Page 34: Monitoring Detector Leds

    Ensure the detector cell is filled with solvent and free of bubbles. The detector can fail to initialize correctly if the cell Caution: contains air. To avoid damaging the light-guiding flow cell, do not ignite the detector lamp when no solvent is flowing through the cell or when it is dry.

  • Page 35: About The Detector Control Panel

    Lamp LED indications (Continued) LED mode and color Description Flashing red Indicates an error stopped the detector. Information regarding the error that caused the failure can be found in the console. Constant red Indicates a detector failure that prevents further operation.
  • Page 36 The following table lists the items in the detector control panel. Modifiable detector control panel items Control panel item Description Lamp On/Off LED Displays the actual lamp on/off LED on the front panel of the detector unless communications with the detector are lost.

  • Page 37: Shutting Down The Detector

    You can access additional functions by right-clicking anywhere in the detector control panel. Additional functions in the detector control panel Control panel function Description Autozero Resets the detector offsets. Reset TUV Resets the detector, when present, after an error condition. Help Displays the console Help.

  • Page 38: Shutting Down For More Than 24 Hours

    Flush the column and flow cell with 100% pure organic solvent. Waters ACQUITY UPLC BEH Column Care and Use See also: Instructions or ACQUITY UPLC HSS Column Care and Use Instructions. Risk of electric shock. The power switch on each system Warning: instrument controls the basic operational state of that instrument.

  • Page 39: Maintaining The Detector

    Maintaining the Detector Contacting Waters technical service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800 252-4752). Otherwise, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. Our Web site includes phone numbers and e-mail addresses for Waters locations worldwide.

  • Page 40: Maintenance Considerations

    Maintenance considerations Safety and handling Observe these warning and caution advisories when you perform maintenance on your detector. To prevent injury, always observe good laboratory practices Warning: when you handle solvents, change tubing, or operate the system. Know the physical and chemical properties of the solvents you use. See the Material Safety Data Sheets for the solvents in use.
  • Page 41 • To conserve lamp life, extinguish the lamp while leaving the detector running but idle. Note, however, that you should do so only when the lamp will remain extinguished more than 4 hours. • If you use buffered mobile phase, flush it from the detector before powering-off to prevent –...

  • Page 42: Maintaining The Leak Sensor

    Nonabrasive, lint-free wipes To resolve a detector leak sensor error View the Leak Sensors dialog box in the ACQUITY UPLC Console to verify that the leak sensor detected a leak. If a leak is detected, a “Leak Detected” error message appears.
  • Page 43 To avoid damaging the leak sensor, do not grasp it by the Caution: ribbon cable. Remove the leak sensor from its reservoir by grasping it by its serrations and pulling upward on it. Serrations If you cannot easily manipulate the leak sensor after removing it Tip: from its reservoir, detach the leak sensor connector from the front of the instrument (see the figure on...
  • Page 44 Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. Rolled up lint-free wipe Leak sensor reservoir With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area.
  • Page 45 If you detached the leak sensor connector from the front of the instrument, reattach it. 10. In the ACQUITY UPLC Console, select your detector from the system tree. 11. In the detector information window, click Control > Reset to reset the detector.

  • Page 46: Replacing The Detector's Leak Sensor

    Replacing the detector’s leak sensor The leak sensor and its reservoir can be contaminated Warning: with biohazardous and/or toxic materials. Always wear clean, chemical-resistant, powder-free gloves when performing this procedure. Required materials • Clean, chemical-resistant, powder-free gloves • Leak sensor To replace the detector leak sensor Open the detector door, gently pulling its right-hand edge toward you.
  • Page 47 Remove the leak sensor from its reservoir by grasping it by its serrations and pulling upward on it. Serrations Unpack the new leak sensor. Maintaining the Detector...

  • Page 48: Maintaining The Flow Cell

    Leak sensor installed in reservoir Plug the leak sensor connector into the front of the instrument. In the ACQUITY UPLC Console, select your detector from the system tree. In the detector information window, click Control > Reset to reset the detector.
  • Page 49 Light transmission through a light guiding flow cell Light path Teflon AF α Mobile phase Teflon AF Teflon AF In the figure above, the light path through the cell is depicted by a pair of rays (dashed lines) that bounce off the cell wall. The energy carried by each ray is conserved after each bounce.
  • Page 50 finish (which is never 100% reflective), or the slow build-up of contaminants. The Teflon AF surface, however, is mirror-like, and the relatively slight RI-dependence associated with a well-maintained cell is negligible. Surface contamination, depicted by the red, irregularly shaped object in the next figure can lead to undesirable beam effects like scattering (dashed arrows) or absorption (gray, thick arrow), both of which decrease energy relative to the incident ray (black, thick arrow).
  • Page 51 flush (see “Performing a system, acid-cleansing flush” on page 43). If you still observe no improvement, call Waters Technical Service. To prevent flow cell failure, do not connect any tubing or device Caution: that can create backpressure exceeding the flow cell’s maximum rating of 6895 kPa (69 bar, 1000 psi).
  • Page 52 If another instrument is downstream of the flow cell outlet, break the connection at the other instrument, and route the outlet tubing to waste while flushing. Do not flush while connected to a mass spectrometer. Caution: Lamp Outlet tubing Flow cell ID connector Thumbscrews Lamp ID Handle...
  • Page 53 10. Reattach the column. 11. Resume pumping mobile phase. If the mobile phase is not miscible in water, first flush with an Tip: intermediary solvent. Performing a system, acid-cleansing flush If you are running a mass spectrometer, do not perform the Caution: system, acid-cleansing flush.
  • Page 54 To perform the system, acid-cleansing flush Failure to remove the bottle filters contaminates the flow Caution: path. Remove the sample and solvent manager bottle filters. Place all lines A1, A2, B1, B2 seal wash, weak needle wash and strong needles in 50:50 methanol:water. Prime the solvent lines for 5 minutes each.
  • Page 55 15. Repeat steps 1 through 8 using 100% water as the solvent. 16. Repeat steps 1 through 8 using 50:50 (v/v) methanol/water as the solvent. 17. Replace the sample and solvent manager bottle filters. Replacing the flow cell Caution: • To avoid contaminating the flow cell, wear clean, chemical-resistant, powder-free gloves when handling, removing, or replacing it.
  • Page 56 Disconnect the detector’s inlet and outlet tubing from the main column connection. Flow cell ID connector Lamp Outlet tubing Thumbscrews Handle Lamp ID Flow cell assembly Leak sensor Backpressure Inlet tubing TP03261 regulator Disconnect the flow cell ID connector (if present). Remove the flow cell: •...
  • Page 57 • Grasp the handle and gently pull the assembly toward you. Handle Thumbscrews TP03261 To avoid damaging the capillary tubing, do not touch it. Caution: Unpack and inspect the new flow cell, ensuring the flow-cell type is correct for your application. When replacing the flow cell, replace the flow cell inlet tubing with Tip: the tubing included with the new flow cell (see...
  • Page 58 Square the flow cell assembly in front of the opening, and then insert it slowly so that the guides on the front part of the cell flange engage the rails in the sample cell compartment. Rail Dowel pin Guide Flow cell handle TP03262 After the flange and rails are engaged, continue inserting the flow cell until the dowel pins on the instrument engage the corresponding holes...

  • Page 59: Replacing The Lamp

    Replacing the lamp Change the lamp when it repeatedly fails to ignite or when the detector fails to calibrate. The ACQUITY TUV lamp is automatically sensed upon installation, and Tip: its serial number and installation date are automatically entered into the Lamp Change Record table.
  • Page 60 The lamp and lamp housing may be hot. Wait 30 Warning: minutes (or 15 minutes with the fan running) for these components to cool before touching them. Allow the lamp to cool for 30 minutes (or 15 minutes with the fan running), and then open the door, gently pulling its right edge toward you.
  • Page 61 Lamp gas is under slight negative pressure. To prevent Warning: shattering the glass, use care when disposing of the lamp. Do not touch the glass bulb of the new lamp. Dirt or Caution: fingerprints adversely affect detector operation. If the bulb needs cleaning, gently rub it with ethanol and lens tissue.

  • Page 62: Replacing The Fuses

    Replacing the fuses To avoid electric shock, power-off and unplug the Warning: detector before examining the fuses. For continued protection against fire, replace fuses only with those of the same type and rating. The detector requires two 100 to 240 Vac, 50 to 60 Hz, F 3.15-A, 250-V FAST BLO, 5 ×...

  • Page 63: Cleaning The Instrument's Exterior

    Make sure that the new fuses are properly rated for your requirements, and then insert them into the holder and the holder into the power entry module, gently pushing until the assembly locks into position. Reconnect the power cord to the power entry module. Cleaning the instrument’s exterior Use a soft cloth, dampened with water, to clean the outside of the detector.

  • Page 64: Error Messages

    Error Messages The detector provides error messages to help troubleshoot system problems. Startup error messages Startup diagnostic tests run automatically when you power-on the detector. They verify the proper operation of the detector electronics. If one or more of the tests fail, the detector beeps and displays an error message. For serious errors, it displays the word “Error”...
  • Page 65 The table below provides startup, calibration, and operating error messages, descriptions, and recommended actions you can take to correct the problem. These messages appear in the console log. Startup, calibration, and operating error messages Error Message Description Corrective Action Calibration differs: n At startup, the unit 1.
  • Page 66 Startup, calibration, and operating error messages (Continued) Error Message Description Corrective Action Lamp lighting failure The lamp failed to 1. Cycle power to the ignite. detector. 2. Check lamp power connection. 3. Replace lamp. Lamp memory device Unable to Change lamp. not detected electronically communicate to lamp...
  • Page 67 Startup, calibration, and operating error messages (Continued) Error Message Description Corrective Action Wavelengths span In dual-wavelength Select wavelengths that are 370 nm: Order filter mode: each above or below 370 not in use • If both selected wavelengths > 370 nm, the detector applies the second-order filter to...

  • Page 68: Error Messages Preventing Operation

    Error messages preventing operation During initialization, calibration, and operation, the detector can display “<Error>” in the control panel, signifying a usually terminating malfunction and preventing further operation of the detector. When you encounter such an error, ensure that • the flow cell is clean. •...
  • Page 69 Instrument error messages (Continued) Error Message Description Corrective Action Electronic A/D failure Lamp optimization is Cycle power. adjusted at the minimum level. Data acquisition via A/D 1. Cycle power to the converters is detector. interrupt-driven. If 2. Contact Waters interrupt is too long, Technical Service.
  • Page 70 Instrument error messages (Continued) Error Message Description Corrective Action Grating initialization Backlash is the 1. Cycle power to the failure: Backlash too difference between the detector. high forward and reverse 2. Contact Waters peak positions of Technical Service. deuterium at 656 nm. If this difference is greater than 1 step, the unit displays this message.
  • Page 71 Instrument error messages (Continued) Error Message Description Corrective Action Thermal controller Thermal controller was 1. Cycle power to the disabled disabled. detector. 2. Contact Waters Technical Service. Vcc fuse failed Fuse failure was 1. Cycle power to the detected. detector. 2.

  • Page 72: Safety Advisories

    Safety Advisories Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them.
  • Page 73 (Risk of injury caused by moving machinery.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) Warning: (Risk of personal exposure to laser radiation.) Warning: (Risk of exposure to biological agents that can pose a serious Warning: health threat.) Specific warnings...
  • Page 74 Mass spectrometer flammable solvents warning This warning applies to instruments operated with flammable solvents. Where significant quantities of flammable solvents are Warning: involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 690 kPa (6.9 bar, 100 psi) during an analysis in which flammable solvents are used.

  • Page 75: Caution Symbol

    Biohazard warning This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans. Waters instruments and software can be used to analyze or Warning: process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials.

  • Page 76: Warnings That Apply To All Waters Instruments

    Warnings that apply to all Waters instruments When operating this device, follow standard quality control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
  • Page 77 Warning: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. • Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
  • Page 78 Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante. • Non utilizzare tubi eccessivamente logorati o piegati. •...
  • Page 79 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. • 근처의 화기를 모두 끄십시오. • 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. • 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축...
  • Page 80 Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
  • Page 81 To protect against fire, replace fuses with those of the type Warning: and rating printed on panels adjacent to instrument fuse covers. Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à...

  • Page 82: Electrical And Handling Symbols

    Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse Recycle symbol: Do not dispose in municipal waste.
  • Page 83 Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments. Keep upright! Keep dry! Fragile! Use no hooks! Safety Advisories...

  • Page 84: Specifications

    Specifications This section lists individual operating specifications for the Waters ACQUITY UPLC TUV Detector, as follows: • Operational specifications • Optical specifications Operational specifications Condition Specification Wavelength range 190 to 700 nm Bandwidth <5 nm Wavelength accuracy +1.0 nm Wavelength +0.1 nm...
  • Page 85 Operational specifications (Continued) Condition Specification Optical Component Specifications Lamp source 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000-hour warranty, front accessible Photodiodes 2 silicon photodiodes (matched pair) Second-order filter Automatic for wavelengths > 370nm Wavelength Erbium filter, used at startup or on demand calibration filter Nitrogen purge Purge fitting present on optics bench...
  • Page 86 Operational specifications (Continued) Condition Specification One event output Type: Contact closure Voltage: +30 V Current: 1 A Input voltage: +30 V maximum One event input 100-ms minimum period Dimensions Height 19.3 cm (7.6 inches) Length 61.0 cm (24.0 inches) Width 34.3 cm (13.5 inches) Weight 12.0 kg (26.5 pounds)

  • Page 87: Solvent Considerations

    Solvent Considerations To avoid chemical hazards, always observe Good Laboratory Warning: Practices when operating your system, handling solvents, or changing tubing. See the Material Safety Data Sheets for the solvents you use. Introduction Preventing contamination For information on preventing contamination, refer to Controlling Contamination in Ultra Performance LC/MS and HPLC/MS Systems (part number 715001307), available on the Waters web site (www.waters.com) Clean solvents...

  • Page 88: Solvent Miscibility

    Water Use water only from a high-quality water purification system. If the water system does not deliver filtered water, filter it through a 0.45-µm membrane filter before use. Using 100% water can cause microbial growth. Waters Caution: recommends changing 100% water solutions daily. Adding a small amount of an organic solvent (~10%) prevents microbial growth.
  • Page 89 • changes involving two miscible solvents may be made directly. Changes involving two solvents that are not totally miscible (for example, from chloroform to water), require an intermediate solvent (such as isopropanol). • temperature affects solvent miscibility. If you are running a high-temperature application, consider the effect of the higher temperature on solvent solubility.
  • Page 90 Solvent miscibility (Continued) Boiling Miscibility λ Cutoff Polarity Viscosity Solvent point °C number index CP, 20 °C (nm) (1 atm) 2-propanol 2.35 117.7 ––- Methyl acetate 0.45 56.3 15, 17 Methyl ethyl ketone 0.43 80.0 Cyclohexanone 2.24 155.7 Nitrobenzene 2.03 210.8 14, 20 ––...

  • Page 91: Wavelength Selection

    How to use miscibility numbers Use miscibility numbers (M-numbers) to predict the miscibility of a liquid with a standard solvent (see “Solvent miscibility” on page 78.) To predict the miscibility of two liquids, subtract the smaller M-number value from the larger M-number value. •...
  • Page 92 UV cutoffs for common solvents The table below shows the UV cutoff (the wavelength at which the absorbance of the solvent is equal to 1 AU) for some common chromatographic solvents. Operating at a wavelength near or below the cutoff increases baseline noise because of the absorbance of the solvent.
  • Page 93 Mixed mobile phases The table below contains approximate wavelength cutoffs for some other solvents, buffers, detergents, and mobile phases. The solvent concentrations represented are those most commonly used. If you want to use a different concentration, you can determine approximate absorbance using Beer’s Law, because absorbance is proportional to concentration.
  • Page 94 Wavelength selection for chromophore detection Certain functional groups found in most compounds absorb light selectively. These groups, known as chromophores, and their behavior can be used to categorize the detection of sample molecules. The table below lists some common chromophores, and their detection wavelengths ( ), as well as the molar absorptivity (ε...
  • Page 95 Inc., 1981. Reprinted by permission of Wadsworth Publishing Co., Belmont, California, 94002. For further details on solvent recommendations, common solvent See also: properties, solvent stabilizers, and solvent viscosity, consult the ACQUITY UPLC System Documentation CD or the ACQUITY UPLC H-Class System Documentation CD. Solvent Considerations...
  • Page 97 Index absorbance damage, reporting halted by fatal error design absorbance screen electronic error message optical algorithms detector analytes control panel, using additional information on description – comparing flow cell, replacing dissolved flushing automatic second-order filter fuses, replacing autozero control I/O signal connector installing lamp backpressure regulator...
  • Page 98 drip management system, proper placement for grating, diffraction dual wavelength mode description handling symbols parameters I/O signal connector, detector electrical installing specifications detector electrical symbols lamp electricity source, connections multi-detector drip tray entrance slit environmental specifications equipment guidelines lamp error messages –...
  • Page 99 multi-detector drip tray, installing fuses lamp reset control, detector noise filtering safety advisories safety considerations, maintenance operating sample photodiode in dual wavelength mode sample scan in single wavelength mode definition specifications – second-order filter optical sensitivity and electronic design setting specification component specifications shutting down optics...
  • Page 100 sensitivity setting display wavelength accuracy range specifications wavelength range selection – spectral features spectrum scanning zero scan startup definition errors symbols caution electrical handling warning system setup shutting down specifications – system acid cleansing flush, performing theory of operation – TUV detector lamp turn on/turn off control...

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