Agilent Technologies InfinityLab LC Series User Manual page 100

4 Preparing the Sampler/Collector
Preparative Sampler Method Parameter Settings
Plug Setting 2
Figure 17
The Post-sample Plug can either be a pure organic solvent or a mixture of organic
solvent and water. It provides a higher transport capacity of sample through the
tubing to the injector valve for cases where the polarity of the analytes and of the
loop content, which is based on the start gradient, do not match. Further, high
concentrated samples require the usage of post-sample plugs due to their
elevated transport capacity.
The Sample plug is followed by an optional three-part Post-sample Plug and
Positioning Plug. The Post-sample Plug and Positioning Plug use a different
solvent from the Loop Solvent, which is the current solvent composition from the
method. All volumes except the Sample injection volume and the Post-sample
Plug are fixed. Specify the total volume of the Post-sample Plug in the adjacent
field; the volume you specify is divided equally between the parts. Click the
down-arrow and specify the location of the solvent for the Post-sample Plug from
the drop-down list; you can select from Location, Current Container, Sample+ and
Location+. Add a comment in the Comment column, if necessary. The Comment
is limited to 20 characters; however, the use of some special characters may
reduce this limit. Clear the check box to remove the Post-sample Plug and
Positioning Plug.
1290 Infinity II Preparative Open-Bed Sampler/Collector User Manual
Plug Setting 2
100