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A-M Systems 2400 Instruction Manual

Patch clamp amplifier
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  • Page 2 INSTRUCTION MANUAL PATCH CLAMP AMPLIFIER MODEL 2400 Each Patch Clamp Amplifier is delivered complete with: A headstage probe (purchased separately) Model Cell 5” Model Cell connector cable Rack Mount Hardware Version 14.0 March 2020 A-M Systems, Inc. PO Box 850 Carlsborg, WA 98324 U.S.A.
  • Page 3 WARNING The Caution symbol calls (http://www.a-msystems.com) Do not attach a line voltage that attention to a procedure or or contact A-M Systems, Inc. does not match the line voltage practice, which, if not correctly directly. specified on the rear panel.

  • Page 4: Table Of Contents

    Table of Contents Table of Contents ........iv Membrane Current Output Range ..39 List of Tables and Figures......v Membrane Voltage Output Range ..39 About This Manual ........1 Filter ............39 Chapter Overview ........1 RMS Meter..........39 Conventions ..........

  • Page 5: List Of Tables And Figures

    ........10 Figure 39. Model Cell ......32 Figure 7. Whole cell recording before Figure 40. Model 2400 Block Diagram ... 35 capacity compensation ...... 14 Figure 41. Probe block diagram ....36 Figure 8. Whole cell recording with fast transients cancelled ......

  • Page 6: About This Manual

    This manual is designed to provide the researcher with the basic features of Model 2400 in order to proceed to experiments as quickly as possible. For this reason we have left the detailed descriptions of each function towards the end of the manual.

  • Page 7: Quick Overview

    1 fA to 1 µA can be recorded with outputs of 1mV/nA to 10mV/fA. Unlike most patch clamp amplifiers, the Model 2400 has a voltage follower in the probe. This allows this amplifier to be a true fast current clamp amplifier with no instability.
  • Page 8 Telegraph outputs provide analog voltage equivalents of front panel settings including error conditions, amplifier mode and gain, Cmembrane, RMS noise, and low pass filter cut-off value. These telegraphs allow the Model 2400’s front panel settings to be automatically recorded by your system software.

  • Page 9: Getting Started

    Connect the power cable to the AC POWER INPUT on the rear of the unit and to an approved outlet. Do not turn the Model 2400 on. Connect the probe cable to the PROBE input on the rear of the unit. Connect a BNC cable from the MODE OUPUT to an oscilloscope.

  • Page 10: Functional Test

    You can then refer to the table to determine the value of resistor in each PROBE GAIN setting. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 11: Single Channel Recordings

    In this section we will be using the model cell, but after you become familiar with the controls on the Model 2400, you will be able to substitute a real electrode and cell for the model cell.
  • Page 12 Relectrode cell they represent. BATH Celectrode Equipment you will need in this section is the Model 2400 with probe, the model 2.7pF To Probe GND Rmembrane cell, and an oscilloscope. In a real experiment, you would also need a pulse...
  • Page 13 If that resistor value is 100Meg, then the amplitude of the square wave should be 100mVp-p. Expected observed values are listed in Table 6. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...
  • Page 14 ¸10 or ¸50 depending on the scale factor you want for your signal. If you want to use the internal signal, change the MODE switch to Vcomp. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...
  • Page 15 0mV. Connect a BNC cable from the x10Vm output to Channel 2 of your oscilloscope. Set the voltage range on channel 2 to 1V/division. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 16: Whole Cell Recordings

    Typically in channel recordings you would record both the membrane voltage and current. You can get both of these outputs from the front panel of the 2400. It is advantageous to monitor membrane current at the MODE OUTPUT , rather...
  • Page 17 In our case we will be using the model cell to simulate these conditions. You have two choices for producing the square wave clamping signal. You can either use the Model 2400’s built-in signal by switching the MODE switch to Vcomp, or you can provide your own signal through the EXTERNAL CLAMP SIGNAL BNC.
  • Page 18 If you want to use the internal signal, change the MODE switch to Vcomp. Turn on the model 2400. The signal you see on the oscilloscope should be essentially a flat line at 0VDC with sharp transients at 60Hz.
  • Page 19 Figure 9. Whole cell recording with all transients cancelled 10.m -10.m 8.m 10.m V(VIB)*10 V(10Vc) A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...
  • Page 20 (by decreasing the volts/division) to 0.5V/division. Also, if the transients are hard to eliminate, you may want to turn on the 3kHz filter in the CLAMP SIGNAL section of the Model 2400. Following appropriate compensation, your trace should look like Figure 9. The values of series resistance and capacitance should match the model cell’s electrode...
  • Page 21 Typically in channel recordings you would record both the membrane voltage and current. You can get both of these outputs from the front panel of the 2400. It is advantageous to monitor membrane current at the MODE OUTPUT , rather...

  • Page 22: Current Clamp Recordings

    Current Clamp Recordings Whenever the MODE knob is set to I=0, Iclamp, Iresist, or Ifollow the amplifier is in current clamp mode. In current clamp mode the model 2400 performs like any other intracellular amplifier. The probe becomes a voltage...
  • Page 23 A bridge balance is used to remove the voltage drop due to the electrode that occurs prior to attaching the electrode to a cell. The Model 2400 Bridge Balance is controlled by the knobs and switches located in the area of diagonal lines. Turn on the bridge balance by switching the SERIES RESISTANCE On/Off switch (located in the lower right portion of highlighted field) to On.
  • Page 24 Probe Gain Switch High High Capacity Compensation Whole Cell Resistance Series Resistance Bridge Balance A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 25: Instrument Operation

    Below is a table of the different probes you can purchase from A-M Systems, Inc. and their feedback resistors.

  • Page 26: Power

    DC power to the amplifier. Figure 13. Meter Meter The liquid crystal display (LCD) on the 2400 is a digital voltmeter whose input is determined by METER and MODE positions. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 27: Mode

    ±10V. Mode Figure 14. There are two main modes for the Model 2400; current clamp modes and voltage Mode clamp modes. Voltage clamp modes on the MODE knob all start with V, while current clamp modes start with I. In voltage clamp modes, the signal measured is membrane current.
  • Page 28 For example, if the feedback resistor is 1G and the GAIN knob is set to 20, the current gain on the OUTPUT BNC will be: 1mV/pA*20=20mV/pA. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 29: Capacity Compensation

    However, in voltage clamp modes the PROBE GAIN will affect the gain of the Im output (see the Probe gain section for Im probe gains). A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 30: Whole Cell Compensation

    As a group, these controls are turned on or off by SERIES RESISTANCE:ON-OFF. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...
  • Page 31 Voltage Clamp When the Model 2400 is in a voltage clamp mode, the VOLTAGE CLAMP Figure 25. Voltage clamp series resistance controls section of SERIES RESISTANCE will be activated. The LAGfine and LAGcoarse work together with RsCOMP to provide positive feedback of the membrane current to the clamping signal. Because positive feedback is inherently unstable it is important to use these controls carefully to prevent undesired oscillations in the recording.

  • Page 32: Clamp Signal

    Cell parameters readjusted 200.m Current Clamp When the Model 2400 is in a current clamp mode, then the bridge balance is controlled by WHOLE CELL COMPENSATION: Raccess and SERIES RESISTANCE: CURRENT CLAMP, as indicated by the highlighted area of diagonal lines (Figure 30).
  • Page 33 PROBE GAIN switch and the External toggle switch. Table 15 lists actual scaling factors for all headstages in all modes. A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 34: Probe Input

    The probe must be connected to the probe input for the Model 2400 to function properly. Be certain to fully tighten the screws on the probe cable to securely fasten the probe connector to the probe input.

  • Page 35: Bath Input

    Current clamp modes 0.01 mV/pA 0.02 mV/pA 0.05 mV/pA 0.1 mV/pA 0.2 mV/pA 0.5 mV/pA A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...
  • Page 36 Mode telegraph output Mode Mode MODE Output Vtest Vcomp 0.8V Vclamp 1.8V 2.8V Iclamp 3.8V A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 37: Ac Power Input

    (mains) power cord. The mains plug must only be inserted in a socket outlet with a protective earth contact. Model Cell The model cell is used for testing and learning how to use the Model 2400 patch Figure 39. Model Cell clamp amplifier.

  • Page 38: Connecting To A Computer

    Connecting to a Computer The Model 2400 has been designed with many features that allow it to be used with several different types of computer interfaces The scaled and fixed outputs can be connected to the A/D converter inputs while the D/A output is connected to the EXTERNAL input.

  • Page 39: Troubleshooting

    Troubleshooting If the Model 2400 does not function properly, consult the following list that suggests solutions to the most common problems. If you need further assistance, please contact customer service at A-M Systems, Inc. Problem Cause/Solution Table 21 Common Problems Error Lights The corresponding function is at saturation.
  • Page 40 Figure 40. Model 2400 Block Diagram A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 41: Circuit Description

    A full schematic package can be purchased from A-M Systems, Inc. after registering your patch clamp amplifier online at: http://www.a-msystems.com. Figure 40 is a block diagram of the Model 2400 illustrating how each of the following sections are connected and function together as a unit. All switching...

  • Page 42: Membrane Voltage Output

    A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 43: Specifications

    3.4” (85mm) long x 0.8”(20mm) high x 0.8”(20mm) wide BNC input connector 1mm ground pin connector A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 44: Maximum Instrument Noise

    Voltage clamp: FAST1: 0-10pF, 0.2-2µs FAST2: 0-10pF, 0.05-1ms Current clamp FAST1: 0-10pF No FAST2 A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 45: Whole Cell Compensation

    4nA p-p at 60Hz in Low Probe Gain Vcomp Command ±5mV at 60Hz Iresist Command ±1nA at 60Hz A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 46: External Command: Voltage Clamp

    Line frequency and Voltage as indicated on the rear panel, either: 120V at 60Hz, or 240V at 50Hz Fuse: 2A 250V slow-blow 5x20mm A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525...

  • Page 47: Physical Dimensions

    Powered Probe Input Pin # Functional Description +15V @100mA Bath input Ground Ground -15V @100mA A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...

  • Page 48: Warranty

    Any postage, shipping and insurance charges incurred in shipping the hardware to A-M Systems must be prepaid by the Purchaser, and all risk for the hardware shall remain with Purchaser until A-M Systems takes receipt of the hardware. Upon receipt, A-M Systems will promptly repair or replace the defective unit and then return the hardware (or its replacement) to the Purchaser with postage, shipping, and insurance prepaid by the Purchaser.
  • Page 49 What are the limits of liability for A-M Systems under this warranty? A-M Systems shall not be liable for loss of data, lost profits or savings, or any special, incidental, consequential, indirect or other similar damages, whether arising from breach of contract, negligence, or other legal action, even if the company or its agent has been advised of the possibility of such damages, or for any claim brought against you by another party.

  • Page 50: Index

    5, 10, 11, 15, 16, 17, 19, 24, 25 single channel recording, 6 specifications, 40 SpeedTest, 3 A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...
  • Page 51 36 whole cell compensation, 15, 26, 27, 28 whole cell recording, 11 Vclamp, 3 A-M Systems, Inc. 131 Business Park Loop, PO BOX 850, Carlsborg, WA 98324 USA Telephone: 800-426-1306 * 360-683-8300 * FAX: 360-683-3525 E-mail: sales@a-msystems.com * Website: http://www.a-msystems.com...
  • Page 52 A-M Systems 2400 Patch Clamp Manual DRW-5027600 Rev 14 6/30/06 Initial Document Control Release 4/28/10 DCR 201200 Warranty and Company info 9/7/10 DCR 201318 New 100 times smaller current clamp 1/21/19 DCR 202615 Update manual content. 03/19/20 DCR 203316. Updated Warranty A-M Systems, Inc.

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