cytiva Amersham ImageQuant 800 Operating Instructions Manual
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cytiva Amersham ImageQuant 800 Operating Instructions Manual

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Operating Instructions
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ImageQuant 800

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  • Page 1 Amersham ImageQuant 800 Operating Instructions Original instructions Amersham ImageQuant 800 cytiva.com...

  • Page 2: Table Of Contents

    Accessories ..............................42 Upgrade options ............................. 44 Installation ......................47 Site requirements ............................48 Instrument setup ............................52 Amersham ImageQuant 800 control software installation ............53 Moving the Amersham ImageQuant 800 .................... 54 System setup ..............................55 4.5.1 Connections ..............................56 4.5.2...
  • Page 3 Regulations for North America ....................... 120 8.3.5 Regulatory statements ..........................121 8.3.6 Declaration of Hazardous Substances (DoHS) ................122 8.3.7 Other regulations and standards ......................124 Health and Safety Declaration Form ...................... 125 Index ........................... 127 Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 4: Introduction

    This chapter contains important user information, descriptions of safety notices, regu- latory information, and a general description of the intended use of Amersham™ ImageQuant™ 800. In this chapter Section See page Important user information About this manual Associated documentation Abbreviations Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 5: Important User Information

    Prerequisites In order to operate Amersham ImageQuant 800 in the way it is intended, the following prerequisites must be fulfilled: • The user must read and understand the Safety Instructions chapter in the Oper- ating Instructions.

  • Page 6: About This Manual

    CONNECT software. If you work in a regulated environment and have purchased and installed Amersham ImageQuant 800 GxP license for Amersham ImageQuant 800 control software, refer to Amersham ImageQuant 800 GxP User Manual (29620391) for additional informa- tion as software functions can vary.
  • Page 7 Note: A note is used to indicate information that is important for trouble-free and optimal use of the product. Tip: A tip contains useful information that can improve or optimize your proce- dures. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 8: Associated Documentation

    Introduction This section describes the user documentation that is delivered with the product, and how to find related literature that can be downloaded or ordered from Cytiva. System-specific documentation The user documentation listed in the table below is available on the web in PDF format.

  • Page 9: Abbreviations

    Non-parallax Non-parallax Optical densiometry Optical densiometry Operating system Operating system Personal computer Personal computer Red Green Blue Red Green Blue SNOW Signal to Noise Optimization Watch Signal to Noise Optimization Watch Ultra-violet Ultra-violet Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 10: Safety Instructions

    Safety precautions Labels Emergency procedures Important WARNING Before installing, operating or maintaining the product, all users must read and understand the entire contents of this chapter to become aware of the hazards involved. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 11: Safety Precautions

    2.1 Safety precautions Safety precautions Introduction Amersham ImageQuant 800 is powered by mains voltage and handles materials that can be hazardous. Before installing, operating, or maintaining the system, you must be aware of the hazards described in this manual. Follow the instructions to avoid injury to the operator or other personnel, damage to samples or other substances handled by the equipment, to the product, or to other equipment in the area.
  • Page 12 Accessories. Use only accessories supplied or recommended by Cytiva. WARNING Do not use Amersham ImageQuant 800 if it is not working prop- erly, or if it has suffered any damage, for example: • damage to the power cord or its plug •...
  • Page 13 Flammable liquids. This product is not approved for handling liquids under conditions where they could be flammable. Installing and moving the product WARNING The product must be installed and prepared by Cytiva personnel or a third party authorized by Cytiva. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 14 2 Safety Instructions 2.1 Safety precautions WARNING Heavy equipment. Contact a Cytiva service person before lifting or transporting the instrument. CAUTION Amersham ImageQuant 800 must be secured before transport or relocation. Contact Cytiva for instructions before transporting the instrument. CAUTION The product is designed for indoor use only.
  • Page 15 70% of relative humidity) or dusty environments. This can result in fire or electric shock. WARNING In case of thunderstorm, do not touch the power supply plug, as this can result in electric shock. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 16 Handle the sample trays and NP lens with care to avoid dropping them. CAUTION Do not place heavy objects on top of the instrument. CAUTION Do not touch the light source in the instrument. The light source may be hot. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 17 Do not add and/or remove any files in the folder generated by Amersham ImageQuant 800 with Amersham ImageQuant 800 control software and Amersham ImageQuant 800 CONNECT. Do not modify any folders in which files have been added or removed. This may prevent correct image capture and analysis afterwards.
  • Page 18 Wear gloves when handling filters to avoid dirtying or scratching the filter. Maintenance WARNING Decontaminate before maintenance. To avoid personnel being exposed to potentially hazardous substances, make sure that the instrument is properly decontaminated and sanitized before main- tenance or service. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 19 2 Safety Instructions 2.1 Safety precautions WARNING Electrical shock hazard. All instrument repairs or modifications should be performed by service personnel authorized by Cytiva. Do not open any covers or replace parts unless specifically stated in the user documentation. WARNING Do not use excessive amounts of liquids for cleaning the Amersham ImageQuant 800 instrument.

  • Page 20: Labels

    • Supply Voltage (VAC Max. • Max. Current (VA) Current Alternating current. Protection Degree of protection provided by the enclosure. Class Manufac- Year (YYYY) and month (MM) of manufacture. turing Year and Month Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 21 The following labels are positioned on the inside of the instrument cabinet. Label Meaning Upper tray position, for Chemiluminescence and White Epi imaging only. Caution, hot surface. Light sources in the instrument may become hot. Lower tray position. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 22 The image below shows the position of the interior labels. Position Label Upper tray label Hot surface caution Lower tray label Position of filter turret label The image below shows the position of the filter turret label that contains the caution notice. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 23 2 Safety Instructions 2.2 Labels Filter labels The illustration below shows the an example of the filter label found on each filter frame. Part Function Filter type Filter product code Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 24: Emergency Procedures

    2.3 Emergency procedures Emergency procedures Introduction This section describes how to shut down the Amersham ImageQuant 800 in an emer- gency situation, and the procedure for restarting the system. The section also describes the result in the event of power failure.
  • Page 25 • If a run was in process, no image data will be saved. Restart after emergency shutdown or power failure To restart the system after an emergency shutdown, follow the startup procedure in Section 5.1 Start the Amersham ImageQuant 800, on page Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 26: System Description

    3 System description System description About this chapter This chapter presents an overview of Amersham ImageQuant 800 features and different instrument configurations. It also contains descriptions of hardware compo- nents, software displays, and functions. In this chapter Section See page...

  • Page 27: Introduction

    3.1 Introduction Introduction The Amersham ImageQuant 800 is an imaging instrument designed to detect and quantify proteins, DNA, and other biological samples, such as bacterial colonies. A cooled 8.3 megapixel CCD camera is used to capture high resolution digital images of electrophoretically separated protein and DNA bands in gels or membranes obtained through blotting methods.

  • Page 28: Illustration Of The System

    3.2 Illustration of the system Illustration of the system The Amersham ImageQuant 800 is operated from an external computer that can either be placed in the VESA™ mount at the rear of the instrument, or separately next to the instrument. The illustration below shows the Amersham ImageQuant 800 setup and the main parts of the system.

  • Page 29: Instrument Description

    Instrument description Main components in each configuration The Amersham ImageQuant 800 is available in different configurations. Different parts and functions are present depending on which upgrade options are used. The table below describes the functions and components of the different instrument configurations.
  • Page 30 Amersham ImageQuant 800 exterior The Amersham ImageQuant 800 is operated from an external computer that can either be placed in the bracket at the rear of the instrument, or separately next to the instrument. The illustration below shows the Amersham ImageQuant 800 setup and the main parts of the system.
  • Page 31 Power socket Connector for attaching AC power cord. Amersham ImageQuant 800 interior The illustration below shows the interior of the ImageQuant 800 instrument. Part Function Upper tray position Lower tray position Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 32 3 System description 3.3 Instrument description Amersham ImageQuant 800 light sources The Amersham ImageQuant 800 houses Epi and Trans light sources. The illustration below shows the location of these light sources. Part Function Epi light source Trans light source Forward Epi light source in the lower tray position is not shown is this illustration.
  • Page 33 CAUTION Changing the filters must be done using the Amersham ImageQuant 800 control software procedure. Do not manually rotate the filter turret. Part Function Filter turret Custom filter holder storage Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 34: Amersham Imagequant 800 Control Software

    3.4 Amersham ImageQuant 800 control software Amersham ImageQuant 800 control software The Amersham ImageQuant 800 control software is designed to guide the user through the workflow of capturing images. The software is operated from a touchscreen on the front of the instrument and is run from an external PC. It is also possible to operate the software from an external monitor that is connected to the PC.
  • Page 35 Options to Shut down the instrument, Minimize application, Logout from the software, show software version information, or show the License agreement. Fluorescence tab. Select for fluorescence imaging. Settings tab. Time and date indicator. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 36 Image library tab SNOW (noise reduction mode) Scheduler tab Time series exposure Settings tab Manual exposure Automatic exposure Help Open saved methods Select binning Return to Home screen Select fluorescence multi- plex Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 37 The Colorimetric tab The illustration below shows the main components of the Colorimetric screen. Part Function Part Function Save image capture settings as a method Open method OD measurement Start capture Gel documentation Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 38 Save image capture settings as a Select dyes method SNOW (noise reduction mode) Select capture area Manual exposure Toggle colorimetric marker Auto exposure Toggle full dynamic range Open method Start pre-capture Help Start image capture Select binning Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 39 Toggle between list and grid (thumb- Selected image informa- nail) view tion Image thumbnails from selected Open selected image folder Select image folder Copy/move image Select imaging method Delete image Sort images Select all images Search images Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 40 Part Function Part Function Service Display Save locations Sleep User accounts Focus Fluorescence dyes Selected settings window Filters Back The Scheduler screen The illustration below shows the main components of the Scheduler screen. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 41 If you work in a regulated environment and have purchased and installed Amersham ImageQuant 800 GxP license for Amersham ImageQuant 800 control software the Login screen and the Home screen will differ. Refer to Amersham ImageQuant 800 GxP User Manual (29620391) for the instructions on the GxP functions.

  • Page 42: Accessories

    3 System description 3.5 Accessories Accessories Accessories delivered with the instrument The following table shows the accessories for the Amersham ImageQuant 800. Depending on the instrument configuration, the accessories included might vary. Image Accessory Description Black tray Used in the upper or lower tray positions for chemiluminescence, colorimetric, fluores- cence, and plate imaging.
  • Page 43 If an external monitor is to be used, another cable is required to connect the PC to the monitor. The type of cable needed depends on the display ports in the external monitor and PC. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 44: Upgrade Options

    (OD), RGB Epi, and NIR Epi (IRlong and IRshort) Light sources in the Amersham ImageQuant 800 The following image shows the positions of the different light sources available for the Amersham ImageQuant 800. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 45 In addition to the four main configurations, it is possible to make additional configura- tions by using alternative upgrade paths. The main upgrade paths and optional routes are shown in the illustration below. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 46 3 System description 3.6 Upgrade options Part Function UV module OD module RGB module NIR module Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 47: Installation

    About this chapter This chapter describes site requirements and preparations necessary to perform before installation of the Amersham ImageQuant 800 instrument. In addition, instructions are included for moving the Amersham ImageQuant 800 instrument within the lab or to another building. WARNING The product must be installed and prepared by Cytiva personnel or a third party authorized by Cytiva.

  • Page 48: Site Requirements

    The power cord with plug must always be easy to disconnect. NOTICE This equipment is not intended for use in residential environments and might not provide adequate protection to radio reception in such environments. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 49 • The equipment must not be excessively exposed to vibration or impacts • Exposure to corrosive gas must be avoided Ambient temperature requirements The following table describes the environmental requirements for Amersham ImageQuant 800. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 50 A-weighted sound pressure level LAeq, t ≤ 60 dB, fast (t = 0.25 s 1 m from the instrument) Protection IP21 provided by enclosure Overvoltage Transient overvoltage category II category Rated pollution Pollution degree 2 applied Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 51 5. Do not place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency. External PC requirements The Amersham ImageQuant 800 uses an external PC to run the system software. The table below indicates the minimum system requirements. Parameter Minimum requirements Operating system Windows®...

  • Page 52: Instrument Setup

    4 Installation 4.2 Instrument setup Instrument setup Amersham ImageQuant 800 is initially installed by Cytiva service representatives or a third party authorized by Cytiva. For connecting additional monitors or peripheral equipment, contact Cytiva for instructions. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 53: Amersham Imagequant 800 Control Software Installation

    All tasks related to software installation require a computer account with adminis- trator privileges. Amersham ImageQuant 800 control software is installed by Cytiva service represen- tatives or a third party authorized by Cytiva. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 54: Moving The Amersham Imagequant 800

    4 Installation 4.4 Moving the Amersham ImageQuant 800 Moving the Amersham ImageQuant 800 WARNING Heavy equipment. Contact a Cytiva service person when lifting the instrument. WARNING Do not block vents and make sure that they are kept free of dust and dirt.

  • Page 55: System Setup

    4 Installation 4.5 System setup System setup Introduction This section describes how to connect a computer to the Amersham ImageQuant 800, and set system-specific settings in the Amersham ImageQuant 800 control software. In this section Section See page 4.5.1 Connections 4.5.2...

  • Page 56: Connections

    4.5 System setup 4.5.1 Connections 4.5.1 Connections External connections The Amersham ImageQuant 800 has the following ports to connect to an external PC: • USB2.0 • USB3.0 • HDMI The Amersham ImageQuant 800 can be connected in two configurations: 1. With an external PC 2.
  • Page 57 The illustration below shows the connections between the ImageQuant 800 and an external PC with an external monitor. Part Function External PC fixed on a VESA mount Cable holder HDMI connection USB2.0 connection USB3.0 connection Display connection Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 58 The connection type depends on the monitor setup. An adaptor might be required if the monitor does not have an HDMI port. Connect to the external PC To connect the Amersham ImageQuant 800 to the external PC, use the following procedure. Step...

  • Page 59: Amersham Imagequant 800 Control Software

    Turn off password protection By default, the Amersham ImageQuant 800 control software is password protected. It is possible to turn off the password requirement for the Amersham ImageQuant 800 by disabling the System login. To do this, use the following procedure.
  • Page 60 Slide the System user login ON/OFF toggle to OFF. Close the System user login ON/OFF window by tapping the cross in the top right corner. Result: the System login settings now shows as OFF. Tap Save. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 61 Section 5.4 Shut down the Amersham ImageQuant 800, on page Note: The new setting is available after restarting the Amersham ImageQuant 800 control software. Create a new user New users can be added in the Settings view. To do this, use the following procedure.
  • Page 62 Tap Save. Change a password To change the password of the current user, follow the procedure below. Step Action In the Home screen, tap Settings in the lower right corner of the screen. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 63 Change password screen shows. Enter the Current password for the current user. Enter a New password for the user. Type the password again in the Confirm password space. Tap Add. Tap Save. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 64 Amersham ImageQuant 800 CONNECT File transfer, calendar booking, and image viewing can be performed via another computer on the network using the Amersham ImageQuant 800 CONNECT software. See the Amersham ImageQuant 800 User Manual (29383813). Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 65: Network

    Network Introduction The Amersham ImageQuant 800 uses an external PC to connect to the local network. This enables the user to transfer and access files on remote computers that have Amersham ImageQuant 800 CONNECT software installed. It is also possible to check the instrument status and use the booking calendar from a remote computer via Amersham ImageQuant 800 CONNECT.
  • Page 66 Step Action Click Add instrument. Result: a new line appears on the Instruments list. Enter the Nickname of the new instrument in the new line. Enter the IP Address of the new instrument. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 67 Action Click Save. Remote access via Amersham ImageQuant 800 CONNECT To login to Amersham ImageQuant 800 CONNECT and access the Amersham ImageQuant 800 from a remote computer, use the following procedure. Step Action Double click on the Amersham ImageQuant 800 CONNECT icon.
  • Page 68 4 Installation 4.6 Network Step Action Select the instrument to connect to. Result: the selected instrument Image Library screen shows. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 69: Operation

    About this chapter This chapter describes how to operate the Amersham ImageQuant 800. WARNING Do not operate Amersham ImageQuant 800 in any other way than described in the Operating Instructions. WARNING Never exceed the operating limits stated in this document and on the system label.
  • Page 70 5 Operation In this chapter Section See page Start the Amersham ImageQuant 800 Prepare the instrument Image capture Shut down the Amersham ImageQuant 800 Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 71: Start The Amersham Imagequant 800

    5.1 Start the Amersham ImageQuant 800 Start the Amersham ImageQuant 800 Before starting the system Before starting the Amersham ImageQuant 800, make sure that the instrument is connected to an external PC, see External connections, on page Start the system Follow the steps below to start the Amersham ImageQuant 800.
  • Page 72 Press the power button on the right side of the instrument to activate it. Result: The instrument's self-diagnostic process will initialize. Note: The self-diagnostic process will normally take approximately 5 minutes. Connection to a network may take longer. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 73 The power indicator on the Amersham ImageQuant 800 illuminates blue. If the power indicator on the instrument does not illuminate blue, turn on the Amersham ImageQuant 800 using the power switch on the right side of the instrument. If Login is turned On, enter your Username and Password.

  • Page 74: Prepare The Instrument

    Glass tray Place the tray in the instrument To place the sample on the tray and place the tray in the correct tray position in the Amersham ImageQuant 800, follow the steps below. Step Action Select a tray and accessories suitable for the capturing method. See...
  • Page 75 The image below illustrates the difference between the different field of views for the Lower (1) and Upper (2) tray positions. Shaded areas indicate the area of the high- performance field of view. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 76 The image below shows the Focus view in the system settings. To adjust the focus, follow the steps below: Step Action Open the Settings tab. Select Focus from the left side of the screen. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 77 Tap Save to save the focus settings and return to the previous view. Select filter WARNING Changing the filters must be done using the control software procedure. Do not open the filter door before initiating the process in Amersham ImageQuant 800 control software. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 78 Wear gloves when handling filters to avoid dirtying or scratching the filter. The Amersham ImageQuant 800 contains an accessible filter turret that houses up to seven different filters, including the option for two custom filters. This can be accessed via the door on the right side of the instrument. For more information on the available...
  • Page 79 The filter turret will rotate to allow access to the selected filter. Note: Do not open the filter turret door while the turret is rotating. When the selected filter position is available, unscrew the locking bolt on the side door. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 80 Pull out the filter and place it in the storage compartment. NOTICE Wear gloves when handling filters to avoid dirtying or scratching the filter. Place the new filter in the turret and close the side door. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 81 ImageQuant 800 in the same way as a standard filter as detailed in Select filter, on page To prepare a custom filter for use with the custom filter holder, use the following proce- dure: Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 82 Release the handle so that it returns to its original position. The custom filter is now ready for use. Filter storage The ImageQuant 800 can store one custom filter internally. To access and store a custom filter, use the procedure below. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 83 5 Operation 5.2 Prepare the instrument Step Action Unscrew the locking bolt on the side door. Open the side door. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 84 To change the filter in the custom filter holder, follow the procedure on Prepare a custom filter, on page Place the custom filter holder back in the storage slot. Close the side door. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 85 5 Operation 5.2 Prepare the instrument Step Action Tighten the locking bolt on the side door fingertight. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 86: Image Capture

    Amersham ImageQuant 800 User Manual (29383813). Capture methods The Amersham ImageQuant 800 supports three modes of image capture: chemilumi- nescence, colorimetric, and fluorescence. The main functions and uses of these modes are described in the table below.
  • Page 87 Note: For guides on exposure selection, basic settings and advanced settings, tap the Help button in the top right corner. Under 3.Advanced settings, change the Capture area of the image, if required. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 88 Tap the browse icon to navigate to and select the save location. Tap Save. Manage image files To view and manage saved image files, follow the steps below. For more information on editing and managing images, see the Amersham ImageQuant 800 User Manual (29383813). Step Action Tap the Image library icon.
  • Page 89 Use the menus on right side panel to Edit the image or change its Contrast and Intensity settings. Tap Save to save changes to the image, or Cancel to return to the image capture window. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 90: Shut Down The Amersham Imagequant 800

    5 Operation 5.4 Shut down the Amersham ImageQuant 800 Shut down the Amersham ImageQuant 800 This section describes the shutdown procedure for the Amersham ImageQuant 800. This can be done via the Amersham ImageQuant 800 control software. Log off current user After running an experiment, the user should log out of the Amersham ImageQuant 800 to allow the next user to log in.
  • Page 91 Shut down using the Amersham ImageQuant 800 control software It is recommended to shut down the Amersham ImageQuant 800 from within the Amersham ImageQuant 800 control software. To do this, use the following procedure. Step Action Tap the Exit icon in the top right corner of the Home screen.
  • Page 92 Turn off the Amersham ImageQuant 800 instrument using the power button To turn off the Amersham ImageQuant 800 using the power button, use the following procedure. This method is not recommended in place of the standard shut down procedure. Note: Shutting down the instrument by using the power button will not shut down the external computer.
  • Page 93 This can be set in the Windows OS. Sleep mode After a period of inactivity, the Amersham ImageQuant 800 enters sleep mode. The default time before entering sleep mode is 30 minutes. To adjust this period, use the following steps.
  • Page 94 If you work in a regulated environment and have purchased and installed Amersham ImageQuant 800 GxP license for Amersham ImageQuant 800 control software the exit menu will differ. The following illustration describes the GxP functions of the exit menu.

  • Page 95: Maintenance

    6 Maintenance Maintenance About this chapter For optimal performance, the Amersham ImageQuant 800 and accessories must be maintained regularly. This chapter provides information to enable users and service personnel to clean, maintain, calibrate, and store the product. For all safety warnings, cautions, and notices, see...
  • Page 96 6 Maintenance In this chapter Section See page Cleaning before planned service Instrument Accessories User maintenance Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 97: Cleaning Before Planned Service

    Health and safety declaration forms Health and safety declaration forms are available for copying or printing in the Refer- ence information chapter of this manual, or on digital media supplied with the user documentation. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 98: Instrument

    This may cause damage to the instrument. Use a soft dry cloth to clean the exterior of Amersham ImageQuant 800. For marks that are hard to remove, wipe with a soft cloth slightly dampened with water and a neutral detergent, or 70% ethanol, then wipe with a new clean, dry cloth.

  • Page 99: Accessories

    6.3 Accessories Accessories Introduction Amersham ImageQuant 800 accessories must be cleaned after use. This section describes recommended cleaning agents and how to clean the accessories. Recommended cleaning agents The accessories may be washed with water and one of the following:...
  • Page 100 To clean trays and inserts, wash the accessory in water using a soft sponge that may be soaked in a cleaning agent. After cleaning, rinse the accessory immediately in water, until no cleaning agent remains, and allow to air dry. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 101: User Maintenance

    6.4 User maintenance User maintenance Regular inspections Regularly inspect the Amersham ImageQuant 800 for the following conditions: • The power supply plug is firmly secured in the power outlet. • The power cord and supply plug do not become overheated.

  • Page 102: Troubleshooting

    If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your Cytiva representative for advice. In this chapter...

  • Page 103: Instrument Problems

    Cytiva representative camera head failure has occurred Smoke is emitted from the Turn off the power switch immediately instrument then an instru- and contact your Cytiva representative ment failure has occurred Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 104 The instrument does not The instrument is not Unplug the instrument and check that all appear to respond to soft- connected to the external connections between the instrument and ware control computer external computer are secure Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 105: Image Problems

    The tray is used incor- Rotate the gel sample 90° positioning the Colorimetric Epi image rectly gel so that the lanes of the gel are parallel to the long side of the tray Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 106: Software Problems

    The sample is not in posi- Check the sample position tion The software is non- Transferring large Avoid file transfer during operation responsive amounts of files can impair the system's performance Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 107: Error Messages

    Tap OK. Make sure that system doors Capture was interrupted are firmly shut and take the image again. System error Make a note of the error message and error code. Contact your Cytiva service representative. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 108 The images captured in this session have not been saved. To return to the software and save the image, tap No. To exit the software without saving and new images or methods, tap Yes. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 109: Reference Information

    8 Reference information Reference information About this chapter This chapter lists the technical specifications of Amersham ImageQuant 800. The chapter also includes a chemical resistance guide, recycling information, regulatory information and ordering information, and Health and Safety Declaration form for service.

  • Page 110: Specifications

    Fluor: 1/100 second to 10 minutes.- Resolution: 1/100 second Dynamic range 4.8 orders of magnitude Capture control Binning, crop, time series, SNOW mode Image output Gray scale 16 bit tif Color image jpg Gray scale jpg Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 111 +28°C. Higher temperatures does not allow the CCD to cool down properly (to -25°C). Parameter Requirement Operating temperature/ Temperature: 18°C to 28°C (with temperature fluctua- humidity conditions tion below 10°C per hour or lower) Humidity: 20% to 70% RH (no dew condensation) Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 112 5. Do not install the equipment near a window. Avoid direct sunlight. Make sure that blinds are attached to nearby windows. 6. Do not place objects near the power outlet to allow easy access to the power cord for disconnection in case of emergency. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 113: Recycling Information

    Follow applicable local regulations for recy- cling of batteries and accumulators. Instructions for disposal Follow the instructions below for disposal of the Amersham ImageQuant 800: Step Action Separate all electronic components (terminal strips, power supplies, trans- mitters, pumps, probes / sensors, etc.) from the system cabinet.
  • Page 114 8 Reference information 8.2 Recycling information Step Action Decontaminate the Amersham ImageQuant 800 cabinet following appro- priate procedures depending on what type of environment the unit was located in. Follow local and/or national/federal requirements for disposal of the Amersham ImageQuant 800 cabinet.

  • Page 115: Regulatory Information

    8.3.2 European Union and European Economic Area 8.3.3 Eurasian Economic Union Евразийский экономический союз 8.3.4 Regulations for North America 8.3.5 Regulatory statements 8.3.6 Declaration of Hazardous Substances (DoHS) 8.3.7 Other regulations and standards Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 116: Contact Information

    The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 117: European Union And European Economic Area

    • used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 118: Eurasian Economic Union

    В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Требование Информация Информацию об изготовлении Наименование, адрес и номер См. телефона производителя Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 119 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 120: Regulations For North America

    This section describes the information that applies to the product in the USA and Canada. NRTL certification This symbol indicates that the Amersham ImageQuant 800 has been certified by a Nationally Recognized Testing Laboratory (NRTL). NRTL means an organization, which is recognized by the US Occupational Safety and Health Administration (OSHA) as meeting the legal requirements of Title 29 of the Code of Federal Regulations (29 CFR), Part 1910.7.

  • Page 121: Regulatory Statements

    When used in a residential environment, there is a concern of radio interference. 주의 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 122: Declaration Of Hazardous Substances (Dohs)

    Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected sepa- rately and handled properly after decommissioning. Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 123 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. Amersham ImageQuant 800 Operating Instructions 29372604 AC...

  • Page 124: Other Regulations And Standards

    The safety of the LED light sources in this instrument has been assessed by risk anal- ysis in accordance with EN 61010-1. The Amersham ImageQuant 800, depending on configuration or upgrades, is equipped with some or all light sources in the table below:...

  • Page 125: Health And Safety Declaration Form

    Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
  • Page 126 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.

  • Page 127: Index

    FCC compliance, 120 disposal of electrical compo- Field of view, 75 nents, 113 Filter turret, 33, 78, 81, 82 Regulatory information, 115 custom filter, 81 filter storage, 82 select filter, 78 Safety notices, 11 Amersham ImageQuant 800 Operating Instructions 29372604 AC...
  • Page 128 Signal to Noise Optimization Watch, 86 Site requirements, 49, 111 Space requirements, 48 Specifications, 110 Start up, 71 Tray selection, 74 Typographical conventions, 7 Upgrades, 29, 44, 45 Instrument configurations, light sources, 44 upgrade paths, 45 Amersham ImageQuant 800 Operating Instructions 29372604 AC...
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  • Page 130 © 2020 Cytiva All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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