YSI Professional Plus Calibration Tips
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YSI Professional Plus Calibration Tips

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Summary of Contents for YSI Professional Plus

  • Page 1 Professional Plus Calibration Tips...

  • Page 2: Table Of Contents

    Table of Contents Introduction...........................1 Calibration Worksheet ........................1 Temperature ..........................3 Calibration Tips .........................3 Troubleshooting Tips.........................3 Conductivity ..........................4 Calibration Tips .........................4 Troubleshooting Tips.........................4 pH ..............................6 Calibration Tips .........................6 Troubleshooting Tips.........................6 ORP..............................9 Calibration Tips .........................9 Troubleshooting Tips.........................9 Dissolved Oxygen........................11 Calibration Tips ........................11 Troubleshooting Tips.......................12 Ammonium ..........................15 Calibration Tips ........................15 Troubleshooting Tips.......................16...

  • Page 3: Introduction

    Introduction This guide provides helpful instructions, tips and troubleshooting suggestions for calibrating a Professional Plus instrument. For more detailed information on calibration and information on how to setup and operate a Pro Plus, please refer to the Pro Plus User Manual.
  • Page 4 Date of Calibration: ___________________ Technician: _____________________________________ Instrument Serial Number:______________ Software Revision:______________ Cable Model Number:________________ Temperature Reading__________________ Temperature Accurate: Y DO Sensor in use: Polarographic Galvanic Sensor notated in Sensor menu? Y DO membrane changed? Color of Membrane_____________ Color notated in Sensor menu? Record the following calibration values: Pre Cal After Cal...

  • Page 5: Temperature

    In this case, contact your local YSI Representative or a YSI Authorized Service Center. Other Pro Plus Cables If your temperature sensor is not reading accurately after cleaning around the sensor, contact your local YSI Representative or an YSI Authorized Service Center.

  • Page 6: Conductivity

    CALIBRATION TIPS 1. It is not necessary to calibrate conductivity, specific conductance and salinity. Calibrating one of these parameters will simultaneously calibrate the others. YSI recommends calibrating specific conductance (temperature compensated conductivity) for greatest ease and accuracy. 2. Ensure the conductivity sensor is clean and dry before performing a specific conductance calibration.
  • Page 7 If the conductivity measurement continues to read more than 3 uS/cm without a conductivity/temperature sensor installed, there may be a problem with the cable and/or instrument. In this case, contact your local YSI Representative or a YSI Authorized Service Center.

  • Page 8: Calibration Tips

    The pH calibration should be verified every day the instrument is used. However, a new pH sensor may be capable of holding its calibration for several days. CALIBRATION TIPS 1. If using a pH sensor in a 6051010 or Quatro cable, calibrate the sensor in port 1 prior to calibrating the sensor in port 2.
  • Page 9 Clean and recondition the sensor if a slow response in the field has been reported or if it takes more than 90 seconds to stabilize in pH buffer. If you get an error message during a pH calibration, check the following: 1.
  • Page 10 Cleaning a Sensor Port section of this document before reinstalling the sensor. If your pH sensor is still not calibrating after performing a sensor cleaning, contact your local YSI Representative r a YSI Authorized Service Center.

  • Page 11: Orp

    ORP solution to obtain this information. The Pro Series ORP sensors use a Ag/AgCl 3.5 M KCl reference. Be sure the value you enter is for this type of reference. If using the YSI Zobell calibration solution, the Pro Plus will automatically determine the calibration value.
  • Page 12 port 1 only. Therefore, if the sensor installed in port 1 is not working properly, the readings from the sensor installed in port 2 will be erroneous. 7. If you continue to get error messages during calibration, clean and recondition the sensor per the instructions in the pH Troubleshooting section of this document.

  • Page 13: Dissolved Oxygen

    2 point at full saturation. 2. For both ease of use and accuracy, YSI recommends that you perform a 1 point calibration in water- saturated air. 3. Make sure that there is a good membrane with fresh electrolyte (O2 probe solution) installed on the DO sensor.

  • Page 14: Troubleshooting Tips

    6. If you suspect port contamination, remove the sensor and follow the instructions in the Cleaning a Sensor Port section. 7. If you continue to have trouble calibrating the DO sensor, contact your local YSI Representative or a YSI Authorized Service Center.
  • Page 15 Reconditioning the DO Sensor Polarographic Sensors - Model # 605203 Due to the chemical reaction taking place under the membrane, deposits will form on the gold cathode and silver anode. The gold cathode will begin to appear dull and the silver anode will turn dark in color. This discoloration is normal;...
  • Page 16 Note: Be sure to: (1) Only use fine 400 grit wet/dry sand paper and (2) Sand as mentioned in the above procedures. Not adhering to either of these instructions can damage the electrodes. If this procedure is unsuccessful, as indicated by improper DO sensor performance, contact your local YSI Representative or a YSI Authorized Service Center.

  • Page 17: Ammonium

    Ammonium The ammonium sensor should be calibrated every day the instrument is used. The ammonium sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. Ammonia is calculated from the ammonium, temperature and pH readings. pH greatly affects the ammonia calculation.

  • Page 18: Troubleshooting Tips

    Sensor/Port setup in the instrument, a dirty sensor or bad buffer solution. Preparing Ammonium Calibration solution We recommend using YSI calibration solutions whenever possible. However, qualified users can save cost by following these recipes for 1 and 100 mg/L standards. Other concentrations can be made by altering the amount of ammonium chloride.
  • Page 19 6. If you suspect port contamination, follow the instructions in the Cleaning a Sensor Port section. 7. If you continue to have trouble calibrating the ammonium sensor, contact your local YSI Representative or a YSI Authorized Service Center.

  • Page 20: Nitrate

    Nitrate The nitrate sensor should be calibrated every day the instrument is used. The nitrate sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. CALIBRATION TIPS 1. If using a nitrate sensor with either a pH or ORP sensor on a 6051010 or Quatro cable, install the pH or ORP sensor in port 1 and the nitrate sensor in port 2.

  • Page 21: Troubleshooting Tips

    Preparing Nitrate Calibration Solution We recommend using YSI calibration solutions whenever possible. However, qualified users can save cost by following these recipes for 1 and 100 mg/L nitrate standards. Other concentrations can be made by altering the amount of potassium nitrate. All other concentrations should remain unchanged. It is important to note that some of these chemicals are hazardous and therefore, the standards should only be prepared by qualified chemists in laboratories where proper safety precautions are possible.
  • Page 22 6. If you suspect port contamination, follow the instructions in the Cleaning a Sensor Port section. 7. If you continue to have trouble calibrating the nitrate sensor, contact your local YSI Representative or a YSI Authorized Service Center.

  • Page 23: Chloride

    Chloride The chloride sensor should be calibrated every day the instrument is used. The chloride sensor should only be used in fresh water (salinity < 2 ppt) and to depths of 55 feet (17 meters) of water. CALIBRATION TIPS 1. If using a chloride sensor with either a pH or ORP sensor on a 6051010 or Quatro cable, install the pH or ORP sensor in port 1 and the chloride sensor in port 2.

  • Page 24: Troubleshooting Tips

    6. If you suspect port contamination, follow the instructions in the Cleaning a Sensor Port section. 7. If you continue to have trouble calibrating the chloride sensor, contact your local YSI Representative or a YSI Authorized Service Center.
  • Page 25 Cleaning and Reconditioning the Chloride Sensor The chloride sensor is considered a pellet membrane ISE. As always, when handling sensors, care should be taken to avoid damaging the membrane. This sensor can be regenerated by washing with alcohol and/or gently polishing with fine emery paper in a circular motion to remove any deposits or discoloration, then thoroughly washing with deionized water to remove any debris.

  • Page 26: Installing And Uninstalling Sensors

    Installing and Uninstalling Sensors GENERAL PRECAUTIONS It is important that the entire sensor connector and cable connector be dry when installing, removing or replacing sensors. This will prevent water from entering the port. Once a sensor is removed, examine the connector inside the port.

  • Page 27: Uninstalling Do, Ph, Orp, Ph/Orp And Ise Sensors

    UNINSTALLING DO, PH, ORP, PH/ORP AND ISE SENSORS First, ensure that the entire sensor and cable bulkhead are clean and dry. Remove sensors upside down (facing the ground) to help prevent water from entering the port upon removal. Simply unscrew the sensor from the cable by holding the sensor port end of the cable (bulkhead) in one hand and the sensor in the other hand.

  • Page 28: Cleaning A Sensor Port

    YSI Confidence Solution can be used to check the accuracy and calibration of the conductivity, pH and ORP sensors. However, to maintain the highest accuracy of the instrument, it should not be used to perform a calibration.

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