Molecular Devices SpectraMax Plus 384 User Manual page 12

Chapter 1: Introduction
The temperature sensors detect the temperature of the air inside the chamber, not the
temperature of the samples in the plate. If you use the instrument to warm the samples, use
a seal or lid on the plate to prevent evaporation of the sample. The seal or lid also helps to
maintain a uniform temperature. It can take an hour or more for a prepared sample to
equilibrate inside the plate chamber. You can speed up equilibration by pre-warming the
sample and the assay reagents to the desired temperature before you place the plate in the
chamber. Validate the data quality to determine whether the seal or lid can stay on the plate
for the read.
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