Onda V-11 SCAN Instruction Manual page 27

2 Establish Method
2.1 Quantitation interface, press the button Establish method.
Measurement A=A1: Absorbance is equal to the measured absorbance value of the
measured wavelength 1
A=A1-m*A2: Absorbance is equal to the difference between the absorbance
value of the measured absorbance at the wavelength 1 and the wavelength
2, m is the Coefficient
A=A1/A2: Absorbance is equal to the ratio of the measured absorbance value
of the measured wavelength 1 and 2
Wavelength 1 Measurement wavelength 1
Wavelength 2 Measurement wavelength 2
Fitting LIN-0: Linear to zero
LIN: Linear.
QUA: Quadratic.
Unit - (No Unit), %, ppm, ppb, g/L, mg/L, μg/L, ng/L, g/dL, mg/dL, μg/dL, mg/mL,
μg/mL, ng/mL, μg/μL, ng/μL, mol/L, mmol/L, IU, Custom(User input, Up to 8
characters).
Calibration Coe K: Input equation coefficient.
Std M: Measure standard sample(s)
Std I: Input standard sample(s)
Standard Standard sample number (Up to 10)
quantity
2.2 Press the item to set measurement parameters.
2.3 After all the parameters are set up, press the button Next to start establishing the
standard curve. If the item Calibration is set to the parameter Coe K, Std M or Std I,
please refer to 2.3.1, 2.3.2 or 2.3.3.
Coe K Input equation coefficient to establish standard curve.
2.3.1
(1) Input coefficients of the equation Concentration = f (Absorbace)
K0 = coefficient of the zero degree term (intercepts)
K1 = coefficient of the first degree term
K2 = coefficient of the second degree term
(2) Press button Next to confirm.
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