Perkin-Elmer FL 6500 Installation Instructions Manual
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Perkin-Elmer FL 6500 Installation Instructions Manual

Low temperature cell holder
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09931514A
FL 6500/ 8500 Low Tem perature Cell Holder I nstallation
I nstructions
This instruction sheet describes the installation of this accessory which is used with the FL 6500/8500
Fluorescence Spectrometer.
Read these instructions before you install this accessory.
NOTE:
Contacting P erkinElm er
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
• Easy to install
• Easy to use
• Used for measurement of fluorescence and phosphorescence at a liquid nitrogen temperature
Figure 1 Low Temperature Cell Holder (P/N:N4201045)
PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.

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Summary of Contents for Perkin-Elmer FL 6500

  • Page 1 09931514A FL 6500/ 8500 Low Tem perature Cell Holder I nstallation I nstructions This instruction sheet describes the installation of this accessory which is used with the FL 6500/8500 Fluorescence Spectrometer. Read these instructions before you install this accessory. NOTE:...

  • Page 2: Dimensions And Specifications

    09931514A Dim ensions and Specifications Physical Characteristic Specifications Cooling System Liquid nitrogen Cooling Temperature 77K (-196℃) Liquid Nitrogen Reservoir (L) 0.84 LT Cell Holder Dimensions (mm) 197(W) x 275(D) x 177.5(H) Weight (Kg) 2.48 Accessory Cover Dimensions (mm) 180(W) x 90(D) x 83.5(H) Cover Weight (Kg) 0.22 Configuration of the Low Tem perature Cell Holder...

  • Page 3: Installation

    5mm outer diameter, 3.4mm inner diameter and 7 inches length. I nstallation 1. Prepare the FL 6500/8500 Fluorescence Spectrometer to install this accessory. 2. Connect the instrument power cord and the communication cable. 3. Loosen the accessory fixing bolt and remove the current sample accessory.
  • Page 4 09931514A 5. Remove the current front cover plate. Figure 8 Front Cover Plate 6. After checking the pogo pin position in the sample compartment, place the Low Temperature Cell Holder to be fit into the pogo pin well. Figure 9 Installing the accessory 7.
  • Page 5 09931514A 9. With opening the lid door, then close the sample compartment lid, and then mount the accessory cover. Figure 12 Mounting the accessory cover 10. Connect the gas flow. (Go to page 9 “Purging the instrument.”) Figure 13 Purge gas flow connection 11.
  • Page 6 09931514A Alignm ent When the Low Temperature Cell Holder is installed for the first time and the unexpected peak is shown, NOTE: you must align to achieve the optimal spectrum. This must be performed at room temperature. 1. Loosen the six M4 screws, and then remove the top cover. 2.
  • Page 7 09931514A 5. Prepare a solution of a suitable fluorescent sample. For example, 10 µM Rhodamine 6G in Ethanol. 6. Fill one of the sample tubes to a depth of 35 mm with the sample. 7. Wipe the outside of the tube to dry it, and then insert the tube gently down and close the sample cover.
  • Page 8 09931514A 10. When the measurement is finished, check if the spectrum shape looks similar with the optimal spectrum of #15. If you’ve got the good spectrum, you can skip steps 11 to14. 11. Remove the accessory cover and open the sample compartment lid carefully. 12.

  • Page 9: P Urging The Instrum Ent

    09931514A 15. The following spectrum shows the optimal angle of separation between fluorescence and Rayleigh light. NOTE: If fluorescence and Rayleigh are overlapping, the angle needs to be adjusted. (see step 12) Examples of erroneously measured spectra 16. After achieving the optimal angle, remove the sample tube and the alignment cover. 17.

  • Page 10: Filling The Accessory W Ith Liquid Nitrogen

    09931514A The gas flow must be connected to the one touch fitting on the front cover plate as shown below. Tubing O.D size is 4 Ø. Filling the accessory w ith liquid nitrogen During filling of the liquid nitrogen tank, protective gloves must be worn, and care must be taken not to touch the metal surfaces of the accessory with unprotected hands.
  • Page 11 09931514A 1. During filling, an enclosed Teflon rod should be located in the accessory to avoid icing in the sample holder position. Gently push a Teflon rod into the sample holder. Some resistance will be encountered when the Teflon rod comes into contact with the two clips which NOTE: secure the teflon rod.
  • Page 12 09931514A M easurem ent 1. Open the Spectrum FL software and select a measurement mode. 2. Check the recognition of the accessory. 3. Set up the measurement parameters. 4. Click Save to save the method after setting up the parameters. 5.

  • Page 13: R Em Oving The Accessory

    09931514A NOTE: The circular cross-section of the sample tube can cause stray light to be reflected into the emission light path. Select one of the emission cut-off filters to remove second and third order stray light from the spectra. 13. When the accessory returns to room temperature after use, it can accumulate condensation from water.
  • Page 14 09931514A...

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Fl 8500

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