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Perkin-Elmer RamanStation 400 Series Getting Started Manual

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MOLECULAR SPECTROSCOPY
RamanStation 400 Series
Getting Started Guide

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Summary of Contents for Perkin-Elmer RamanStation 400 Series

  • Page 1 MOLECULAR SPECTROSCOPY RamanStation 400 Series Getting Started Guide...
  • Page 2 Release History Part Number Release Publication Date L1321908 February 2011 Any comments about the documentation for this product should be addressed to: User Assistance PerkinElmer Chalfont Road Seer Green Beaconsfield Buckinghamshire HP9 2FX United Kingdom Or emailed to: info@perkinelmer.com Notices The information contained in this document is subject to change without notice.

  • Page 3: Table Of Contents

    Switching on the Instrument ................48 Moving the Instrument ..................50 Motorized XYZ Stage ..................50 Triggered Probe Holster ................50 Introduction to the RamanStation 400 Series ......... 53 Spectrometer Front Panel .................54 Sample Compartment ..................55 Motorized XYZ Stage Controller and Joystick ............56 Sample Accessories (for Motorized XYZ Stage) ...........57...
  • Page 4 Fiber Optic Probe ..................59 Important: Read This First ................60 Fiber Optic Probe (FOP) ................... 61 General Overview of the Fiber Optic Probe ..........61 Attaching and Removing the Fiber Optic Probe ..........62 Attaching the FOP ..................62 Removing the FOP ..................

  • Page 5: Introduction

    Introduction...

  • Page 6: About This Manual

    This chapter contains a brief overview of the history and theory of Raman spectroscopy. Preparing your Facility This chapter shows you how to position your RamanStation 400 Series instrument, and what utilities are required; for example, electricity and temperature control.

  • Page 7: Conventions Used In This Manual

    ALT+F. All eight-digit numbers are PerkinElmer part numbers unless stated otherwise. The term RamanStation 400 Series means the RamanStation 400 and the RamanStation 400F Raman Spectrometers.
  • Page 8 8 . RamanStation 400 Series Getting Started Guide We use the term CAUTION to inform you about situations that could CAUTION result in serious damage to the instrument or other equipment. Details about these circumstances are in a box like this one.
  • Page 9 Introduction . 9 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING Warning (Warnung) Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann.
  • Page 10 10 . RamanStation 400 Series Getting Started Guide...

  • Page 11: Safety Practices

    Safety Practices...

  • Page 12: Overview

    12 . RamanStation 400 Series Getting Started Guide Overview This chapter contains details of the safety precautions. Everyone who is going to install and/or use the spectrometer must read and follow the precautions before unpacking, installing or using the instrument.

  • Page 13: General Operating Conditions

    50% relative humidity at 40 °C Mains fluctuations not exceeding ±10% of the nominal voltage. • Whenever it is likely that the RamanStation 400 Series instrument is unsafe, make it inoperative. The RamanStation 400 Series may be unsafe if it: •...

  • Page 14: Fire Safety

    14 . RamanStation 400 Series Getting Started Guide Fire Safety Ensure that there is at least 15 cm (6”) between the rear of the instrument and the • nearest wall. Ensure that any power supply unit (PSU) vents or fan do not become obstructed.

  • Page 15: Electrical Safety

    100–230 V AC ±10% of the nominal voltage NOTE: If you have a deep depletion CCD installed in your RamanStation 400 Series, the instrument will be powered by two external PSUs. Under no circumstances should you attempt to disassemble the PSU and carry out repairs.

  • Page 16: Power Cords (Between Psu And Power Outlet Socket)

    16 . RamanStation 400 Series Getting Started Guide Connect the PSUs to a three-wire (live, neutral and ground/earth) socket. The ground must be a wire not carrying current that is connected to the earth ground at the main distribution box. The power points (sockets) for the instrument PSUs should be easily accessible for when the instrument is serviced.

  • Page 17: Emc Compliance

    EMC Compliance EC directive The RamanStation 400 Series instruments have been designed and tested to meet the requirements of the EC directive 2004/108/EC. The RamanStation 400 Series complies with the EMC standard EN61326, (EMC standard for electrical equipment for measurement, control and laboratory use) and EN55011 (ISM) Class A (RF emissions).

  • Page 18: Laser Safety

    18 . RamanStation 400 Series Getting Started Guide Laser Safety The RamanStation 400 Series complies with the requirements of IEC 60825-1:2007 and 21CFR 1040.10 FDA performance standards for laser products, except for deviations pursuant to Laser Notice No. 50, dated 26 July 2001.

  • Page 19: General Laser Safety Issues

    Safety Practices . 19 The following information is provided as a general overview only. This information is rudimentary and is supplied to assist in the interpretation of this manual. Under no circumstances use this information as the basis of a laser safety code of behavior: 1.
  • Page 20 20 . RamanStation 400 Series Getting Started Guide When attached to the spectrometer, the fiber optic probe provides a focused beam of Class 3B laser radiation. During normal operation, laser radiation is accessible from the end of the fiber optic probe. The LED emission indicator (Figure 1) at the probe head is configured so that the LED lights up when the laser is turned on, indicating that laser radiation is emitting from the probe aperture.
  • Page 21 Safety Practices . 21 Triggered Fiber Optic Probe Safety When attached to the RamanStation 400F, a triggered fiber optic probe (TFOP) provides a focused beam of Class 3B laser radiation. During normal operation, laser radiation is accessible from the end of the probe. The following precautions must be observed at all times: •...
  • Page 22 22 . RamanStation 400 Series Getting Started Guide The equation for a focused beam, as given here, is used to calculate the nominal ocular hazard distance (NOHD) for the probe:   Φ       ...

  • Page 23: Internal Interlock System

    Magnetic actuator (MA) Magnetic proximity switch (MPS) Figure 3 The RamanStation 400 Series safety interlock system, showing the proximity of the MPS and MA when the sample lid is closed. Note that the MPS and MA are located inside the instrument enclosure Do not tamper with the safety interlock mechanism.

  • Page 24: External Interlock System

    24 . RamanStation 400 Series Getting Started Guide External Interlock System NOTE: This section is only applicable to systems equipped with a fiber optic probe or RamanMicro 300 Accessory, or systems being used in a Class 3B fashion. Fiber optic probes and the RamanMicro 300 Accessory are Class 3B laser products and have...

  • Page 25: Warning Labels

    Safety Practices . 25 Warning Labels When this label is attached to an instrument it means refer to the manual to find out the nature of the potential hazard and any actions which have to be taken. Labels on the Exterior of the RamanStation 400F The following labels are displayed on the exterior of the instrument.

  • Page 26: Labels On The Exterior Of The Ramanstation 400

    26 . RamanStation 400 Series Getting Started Guide Labels on the Exterior of the RamanStation 400 The following labels are displayed on the exterior of the instrument. Power Max. 100W DC Voltage Current Max. 4A 6.5A CLASS 1 CLASS 1...

  • Page 27: Raman History, Theory And Instrumentation

    Raman History, Theory and Instrumentation...

  • Page 28: Raman Theory

    28 . RamanStation 400 Series Getting Started Guide Raman Theory When photons from a monochromatic light source impinge on a sample, a small fraction are scattered in all directions. Some of the scattered photons are of the same energy as those of the incident beam, and hence the same frequency, giving rise to Rayleigh scattering (Figure 6).
  • Page 29 Raman History, Theory and Instrumentation . 29 The change between the incident radiation and the Stokes/Anti-Stokes radiation represents a change in the vibrational and/or rotational energy of the molecule. The frequency shifts from the frequency of the incident beam are displayed on the x-axis of a Raman spectrum, generally in relative wavenumbers (relative to the laser wavelength), otherwise known as Raman Shift, and the intensity of the bands are displayed in arbitrary units on the y-axis.

  • Page 30: The Ramanstation 400 Series

    30 . RamanStation 400 Series Getting Started Guide The RamanStation 400 Series The RamanStation 400 Series are bench-top spectrometers with laser excitation wavelength at 785 nm. It has a sample compartment with high throughput capabilities. The RamanStation 400F can be used with a fiber optic probe or a RamanMicro 300 Accessory.
  • Page 31 Raman History, Theory and Instrumentation . 31 Optical Head (detailed block diagram below) Video Head Motorized XYZ Stage Laser High Performance TE cooled CCD Detector Spectrograph Figure 10 Schematic of the RamanStation 400 Series Video Camera Beampath Rayleigh Assembly Filters Lens To Spectrograph Beam- Splitter Objective...

  • Page 32: Spectrograph Detector Systems

    32 . RamanStation 400 Series Getting Started Guide Spectrograph Detector Systems The RamanStation 400 Series is equipped with an Echelle spectrograph. The Echelle Spectrograph CCD Detector Lens #2 Grating #2 Grating #2 Lens #1 Figure 12 Schematic of the Echelle spectrograph PerkinElmer’s Echelle detector is shipped in the RamanStation 400 and RamanStation 400F...
  • Page 33 Raman History, Theory and Instrumentation . 33 −1 3000 cm −1 4000 cm −1 2000 cm −1 3000 cm −1 1000 cm −1 2000 cm −1 100 cm −1 1000 cm 1000 Pixels Figure 13 Strips of light on the CCD detector using the Echelle spectrograph Range 1 + Range 2 + Range 3 + Range 4 = Complete Spectrum (all ranges acquired simultaneously).
  • Page 34 34 . RamanStation 400 Series Getting Started Guide...

  • Page 35: Preparing Your Facility

    Preparing Your Facility...

  • Page 36: Specifications

    36 . RamanStation 400 Series Getting Started Guide Specifications Spectrometer RamanStation 400 RamanStation 400F Weight 45 kg 45 kg Height 490 mm 490 mm Width 590 mm 590 mm Depth 640 mm 640 mm Laser Class Class 1 Class 3B Operational Temperature 15 °C –...

  • Page 37: Finding A Suitable Location For The Instrument

    Preparing Your Facility . 37 Finding a Suitable Location for the Instrument The following conditions should be observed: There must be a minimum of 3 power points (1 for the spectrometer; 2 for the PC). If • your system has a deep depletion CCD you will also need 1 power point for the recirculating water cooler.

  • Page 38: Instrument Dimensions

    38 . RamanStation 400 Series Getting Started Guide Instrument Dimensions The RamanStation 400 Series spectrometer weighs approximately 45 kg. The stage can accept loads up to 1.0 kg. Laboratory benching must be strong enough to support the combined weight of the instrument and samples, typically 46 kg, without warping or wobbling.

  • Page 39: Installing Your Ramanstation 400 Series

    Installing Your RamanStation 400 Series...

  • Page 40: Unpacking The Instrument

    40 . RamanStation 400 Series Getting Started Guide Unpacking the Instrument When the instrument has been delivered, please move it with care and avoid shaking or dropping the spectrometer. Allow the instrument to equilibrate to room temperature for at least 12 hours before opening. The instrument may have been stored at low temperature prior to delivery and you should allow it to equilibrate to stop moisture condensing on the internal electronics.
  • Page 41 Deep Depletion PSU L1323010 Recirculating Cooler L1321674 Tygon Tubing NOTE: Your instrument will be assembled and tested by a PerkinElmer Service Engineer. There are no user-serviceable parts in the RamanStation 400 Series. The following sections are provided for your information only.

  • Page 42: Lifting The Instrument

    42 . RamanStation 400 Series Getting Started Guide Lifting the Instrument The RamanStation 400 Series instrument weighs 45 kg. To avoid injury or damage to the instrument, please use proper lifting techniques. To lift and/or move the instrument safely requires two people.

  • Page 43: Preparing The Motorized Xyz Stage

    Before you use your instrument you must remove the fixing bolt that is fitted to the stage during shipping to prevent damage to the stage. Fixing bolt Figure 16 RamanStation 400 Series Stage Fixing Bolt Use a 5 mm hexagonal key to loosen the bolt and retain it for future use. Installing the motorized XYZ stage Open the sample compartment of your RamanStation.
  • Page 44 44 . RamanStation 400 Series Getting Started Guide 2. Connect the three ends of the connector cable to the sockets labeled MOTOR X, MOTOR Y and MOTOR Z on the rear panel of the stage controller box. The three ends of the connector cable are labeled X, Y and Z, as appropriate (Figure 18).

  • Page 45: Attaching The External Interlock Override

    Installing Your RamanStation 400 Series . 45 Attaching the External Interlock Override The RamanStation 400F has two interlock switches, an internal interlock located on the inside of the sample compartment door, and an external interlock located on the rear panel. To operate the instrument both interlocks must be satisfied;...

  • Page 46: Attaching The Fiber Port Cover Assembly

    46 . RamanStation 400 Series Getting Started Guide Attaching the Fiber Port Cover Assembly NOTE: This section is only applicable if you have a RamanStation 400F. The RamanStation 400F is classified as a Class 3B laser product according to the label attached to the instrument.

  • Page 47: Installing The Instrument In The Software

    Installing Your RamanStation 400 Series . 47 Installing the Instrument in the Software You must not connect the instrument USB cable to the PC before the Spectrum software has been installed. NOTE: A PerkinElmer Service Representative will install the Spectrum software on the PC and set up your instrument in the software.

  • Page 48: Switching On The Instrument

    To recirculator From instrument Figure 20 Connecting the water recirculator to the RamanStation 400 Series 4. Connect the instrument power supply (large PSU with 8 pin connector) to the RamanStation 400 MAIN POWER socket and then switch on (Figure 21).
  • Page 49 Installing Your RamanStation 400 Series . 49 5. If appropriate, connect the motorized XYZ stage controller box to a mains power socket and switch it on. The green LED on the controller box will light up.

  • Page 50: Moving The Instrument

    50 . RamanStation 400 Series Getting Started Guide Moving the Instrument The RamanStation 400 weighs about 45 kg. Two people are needed to lift the instrument. WARNING If you need to ship your instrument you must use the original supplied packing materials to prevent damage to the system and follow the procedures described in this section.
  • Page 51 3. Locate and remove the two screws securing the holster bracket to the base of the instrument (located behind the front foot). Right front foot of RamanStation Screws Figure 23 Base of the RamanStation 400 Series instrument 4. Return the instrument to its correct position on your workbench.
  • Page 52 52 . RamanStation 400 Series Getting Started Guide...

  • Page 53: Introduction To The Ramanstation 400 Series

    Introduction to the RamanStation 400 Series...

  • Page 54: Spectrometer Front Panel

    54 . RamanStation 400 Series Getting Started Guide Spectrometer Front Panel The front panel of the spectrometer module contains 3 LED indicators and 1 control button (Figure 24). Collect Spectrum Figure 24 Spectrometer module front panel Description Power If the Power LED is illuminated, this indicates that the instrument is receiving power.

  • Page 55: Sample Compartment

    Introduction to the RamanStation 400 Series . 55 Sample Compartment To open the sample compartment, lift the cover using the handle at the front of the RamanStation 400 Series as shown in Figure 25. Figure 25 Opening the sample compartment of the RamanStation 400 Series...

  • Page 56: Motorized Xyz Stage Controller And Joystick

    56 . RamanStation 400 Series Getting Started Guide Motorized XYZ Stage Controller and Joystick The motorized stage and joystick (L1865723) are powered by the stage controller box (L1860289). Figure 26 Stage controller and joystick NOTE: When the joystick is used to move the stage, care should be taken that excessive movement does not bring the lens snout into forceful contact with the sample or stage, as this may cause damage to both.

  • Page 57: Sample Accessories (For Motorized Xyz Stage)

    Lens snout XYZ stage Figure 27 RamanStation 400 sample compartment The Versatile Sample Holder (L1320227) is supplied with your RamanStation 400 Series (Figure 28). You can use this holder to collect data from microscope slides, cuvettes, sample tubes and capillary tubes.
  • Page 58 58 . RamanStation 400 Series Getting Started Guide Figure 29 Raman Polarization Accessory If you have a RamanStation 400F instrument, you can collect data using a fiber optic probe Fiber Optic Probe (see on page 59 for more details), which enables the remote measurement of samples.

  • Page 59: Fiber Optic Probe

    Fiber Optic Probe...

  • Page 60: Important: Read This First

    60 . RamanStation 400 Series Getting Started Guide Important: Read This First This section is only applicable if you have a RamanStation 400F that is fitted with a fiber optic probe, or is being utilized in a Class 3B mode of operation.

  • Page 61: Fiber Optic Probe (Fop)

    Fiber Optic Probe . 61 Fiber Optic Probe (FOP) Raman Triggered NOTE: For details of how to use a triggered fiber optic probe, see the Fiber Optic Probe leaflet (L1321940). General Overview of the Fiber Optic Probe Your RamanStation 400F spectrometer fitted with a Fiber Optic Probe is a Class 3B laser system.

  • Page 62: Attaching And Removing The Fiber Optic Probe

    62 . RamanStation 400 Series Getting Started Guide Attaching and Removing the Fiber Optic Probe The standard fiber optic probe is constructed such that a single armored cable bifurcates into an excitation cable and a collection cable. The excitation fiber transmits the laser energy, whereas the collection cable is a conduit for the Raman energy.

  • Page 63: Removing The Fop

    Fiber Optic Probe . 63 9. If you have a standard probe, connect the DIN connector to the nearby socket marked LED FIBER #1. If you have a triggered probe, connect the USB extender cable to a USB port on your 10.

  • Page 64: Acquiring Spectra With The Fiber Optic Probe

    64 . RamanStation 400 Series Getting Started Guide Acquiring Spectra with the Fiber Optic Probe The standard Raman fiber optic probe has a tight focus spot that is approximately 7.5 mm away from the lens. The focused spot enables you to pinpoint the measurement area on the sample.
  • Page 65 Fiber Optic Probe . 65 Non Immersion When using 785 nm lasers, many types of glass exhibit fluorescent bands between 1400 ̵ −1 1600 cm . Glass spectra of the container should be obtained before analyzing the sample to ensure that glass-derived fluorescence bands do not pose a problem. With 785 nm excitation, fused silica (quartz) should be used instead of glass where possible as this does not yield fluorescent peaks.
  • Page 66 66 . RamanStation 400 Series Getting Started Guide...

  • Page 67: Using The Ramanstation 400

    Using the RamanStation 400...

  • Page 68: Introduction

    68 . RamanStation 400 Series Getting Started Guide Introduction The RamanStation 400 and RamanStation 400F combined with the Spectrum software are very flexible instruments. The Standard Operating Procedures (SOPs) provided in this chapter demonstrate how to perform a number of tasks, which you can use as a starting point to develop your own SOPs that better suit your specific mode of use.

  • Page 69: General Sops

    Using the RamanStation 400 . 69 General SOPs SOP-1: Turning the Instrument On 1. Switch on the PC and monitor. 2. Login to Windows. 3. Ensure the laser power key is in the ARM position on the rear panel of the instrument. 4.

  • Page 70: Sop-2: Turning The Instrument Off

    70 . RamanStation 400 Series Getting Started Guide 8. From the Start menu select Programs; the PerkinElmer Applications group; the Spectrum sub-group and then the Spectrum application. The Spectrum start-up screen is displayed, followed by a dialog that may require your login details.

  • Page 71: Sop-3: Installing And Changing Sampling Holders

    Sample Accessory port and not to the Auxiliary Control port. Install the 96 well plate 1. Ensure the RamanStation 400 Series is switched on. If not, follow SOP-1. 2. If you did not connect to your instrument on starting Spectrum software, select Instruments from the Setup menu and then Select Instrument from the sub-menu.
  • Page 72 72 . RamanStation 400 Series Getting Started Guide 6. In the Accessory section, ensure that Sample Area XYZ Stage is selected as the Accessory. 7. Ensure the lens snout is pointing towards the sample (the sample holder and snout should point down).
  • Page 73 Using the RamanStation 400 . 73 Install the Versatile Sample Holder (7 mm Sample Bottle) for use with a sample bottle 1. Click Move to remove sample holder on the Setup Sample Area XYZ Stage tab to move the stage to a safe position for you to unload your current sample holder. 2.

  • Page 74: Sop-4: Setting-Up Experimental Parameters And Collecting Data

    74 . RamanStation 400 Series Getting Started Guide 10. Load the sample bottle into the versatile sample holder. 11. Close the sample compartment lid. The instrument can now be used to position the sample (SOP-5), and set up the experimental parameters and collect data (SOP-4).
  • Page 75 Using the RamanStation 400 . 75 −1 8. If required, adjust the Data Interval in Raman Shift (cm −1 The default data interval is 2 cm . The Data Interval can be set on the Setup Instrument Basic tab. 9. If you wish to edit the data collection parameters, such as how background scans are collected, select the Setup Instrument Data Collection tab.

  • Page 76: Sop-5: Sample Positioning Using The Motorized Xyz Stage

    76 . RamanStation 400 Series Getting Started Guide If you exit Spectrum software the instrument settings will be remembered when you restart Spectrum, providing that they have been used to collect data. If you want to return to the default instrument settings, click Restore Defaults on the Setup Instrument Basic tab. If you wish these parameters to be stored as a new set of instrument settings, select Load and Save on the Setup Instrument Basic tab.
  • Page 77 Using the RamanStation 400 . 77 5. Use the joystick to position the stage. The joystick operates in the following manner: – Left / Right joystick movement gives x movement of the stage. – Top / Bottom joystick movement gives y movement of the stage. –...

  • Page 78: Fiber Optic Probe Sops

    78 . RamanStation 400 Series Getting Started Guide Fiber Optic Probe SOPs NOTE: The SOPs included on the following pages are only appropriate for a standard fiber optic probe. For details of how to acquire data using a triggered fiber optic probe, see Raman Triggered Fiber Optic Probe leaflet (L1321940).

  • Page 79: Sop-7: Sample Positioning When Using A Fiber Optic Probe

    Using the RamanStation 400 . 79 SOP-7: Sample Positioning when using a Fiber Optic Probe Monitor allows you to collect spectral data in the Live display while optimizing the probe position, laser power and exposure time to enhance the signal-to-noise ratio of spectral data. When measuring samples inside a container (for example, a bottle) it can be used to ensure that you are focused on the material of interest and not the container.

  • Page 80: Calibration And Performance Verification Sops

    80 . RamanStation 400 Series Getting Started Guide Calibration and Performance Verification SOPs Several different automated calibration tests are included in the software: Wavelength (x-axis) calibration uses the position of precisely known lines in a neon • emission spectrum to calibrate the detector.

  • Page 81: Sop-9: Wavelength Calibration Verification

    If the instrument fails the wavelength calibration verification again, please contact PerkinElmer. SOP-9: Wavelength Calibration Verification 1. Ensure the RamanStation 400 Series is switched on. If not, follow SOP-1. 2. Select Raman Instrument from the Setup menu.
  • Page 82 8. Ensure that the RamanStation 400 Series sample compartment is closed and, if you have a RamanStation 400F, ensure that any external accessory is capped or covered to prevent stray light interfering with the measurement.

  • Page 83: Sop-10: Wavelength Calibration

    RamanStation 400 Series package. The external neon calibration accessory can be purchased separately. 3. Ensure that the RamanStation 400 Series sample compartment is closed and, if you have a RamanStation 400F, ensure that any external accessory is capped or covered, to prevent stray light interfering with the measurement.

  • Page 84: Sop-11: Laser Frequency Calibration

    7. Ensure that the RamanStation 400 Series sample compartment is closed and, if you have a RamanStation 400F, ensure that any external accessory is capped or covered, to prevent stray light interfering with the measurement.

  • Page 85: Sop-12: Intensity Calibration

    Using the RamanStation 400 . 85 12. Ensure that you have followed the instructions on the dialog, and then click Next. The instrument will now perform the laser frequency calibration routine. This will take a few minutes. When the calibration has completed, the result is displayed. The instrument in now laser frequency calibrated.
  • Page 86 7. Ensure that the RamanStation 400 Series sample compartment is closed and, if you have a RamanStation 400F, ensure that any external accessory is capped or covered, to prevent stray light interfering with the measurement.

  • Page 87: Summary

    Using the RamanStation 400 . 87 13. Remove the intensity correction standard and store safely. Any data acquired will now be intensity corrected. In the unlikely event that the intensity calibration routine should fail, please ensure the NIST sample is correctly positioned, and that the laser key switch is set to on (armed), and the laser power is 100%.

  • Page 88: Additional Parts And Upgrades

    88 . RamanStation 400 Series Getting Started Guide Additional Parts and Upgrades Sampling Accessories Part Number Description L1323514 Quartz microscope slide, 25 × 75 × 1 mm L1323515 Quartz capillary tubes, 1.8 mm OD (pack of 25) B0631015 NIR rectangular cell with lid, Quartz SUPRASIL 300, light path 10 mm...

  • Page 89: Sers Consumables

    Using the RamanStation 400 . 89 SERS Consumables Part Number Description L1329300 SERS gold colloid – 50 nm particles (20 ml) L1329307 SERS gold colloid – 100 nm particles (20 ml) L1329308 SERS gold colloid – 200 nm particles (20 ml) L1329301 Aggregating agent –...

  • Page 90: Fiber Optic Probes

    90 . RamanStation 400 Series Getting Started Guide Fiber Optic Probes Part Number Description L1320002 Raman fiber optic probe, 7.5 mm working distance, maximum working temperature 80 °C, 5 m cabling L1320003 Liquid immersion sleeve for Raman fiber optic probe L1320011 Raman probe –...

  • Page 91: Maintenance And Troubleshooting

    Maintenance and Troubleshooting...

  • Page 92: Maintenance

    92 . RamanStation 400 Series Getting Started Guide Maintenance The system does not require regular maintenance other than occasional checking and cleaning. Cleaning the Spectrometer and Power Supply Units Switch off the mains voltage and disconnect the mains cord before cleaning.

  • Page 93: Servicing The Spectrometer

    Maintenance and Troubleshooting . 93 Servicing the Spectrometer With the exception of the fan filter (mentioned above) there are no user serviceable parts on the instrument. Removal of the protective housing or external panels is dangerous and is not recommended for users under any circumstances. If you require an instrument service please contact PerkinElmer.

  • Page 94: Replacing The Stage Controller Fuse

    94 . RamanStation 400 Series Getting Started Guide Replacing the Stage Controller Fuse NOTE: This section is only applicable if you have an motorized XYZ stage. Electrical hazard To prevent potential injury to yourself and damage to the instrument, switch OFF all components in the system and disconnect them from the...

  • Page 95: Troubleshooting

    Maintenance and Troubleshooting . 95 Troubleshooting This section is a reference guide if you are having any problems with your instrument. Troubleshooting outlines what to do if your instrument or software malfunctions or crashes. Hardware Problem: During the installation procedure the USB devices are not recognized.
  • Page 96 On the Setup Sample Area XYZ Stage tab, click Toolbox and then select Reset Stage. The motorized accessory will now reset itself. NEVER attempt to reset the stage with the RamanStation 400 Series lens snout in the Horizontal orientation. Solution 3 If the above solutions do not solve your problem, you should contact your PerkinElmer Service Representative.

  • Page 97: Decontamination

    Maintenance and Troubleshooting . 97 Decontamination Before using any cleaning or decontamination methods except those specified by PerkinElmer, users should check with PerkinElmer that the proposed method will not damage the equipment. Customers wishing to return instrumentation and/or associated materials to PerkinElmer for repair, maintenance, warranty or trade-in purposes are advised that all returned goods must be certified as clean and free from contamination.

  • Page 98: Weee Instructions For Perkinelmer Products

    98 . RamanStation 400 Series Getting Started Guide WEEE Instructions for PerkinElmer Products A label with a crossed-out wheeled bin symbol and a rectangular bar indicates that the product is covered by the Waste Electrical and Electronic Equipment (WEEE) Directive and is not to be disposed of as unsorted municipal waste.

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