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Summary of Contents for Euromex OXION Series
- Page 1 The Netherlands www.euromex.com page1/16...
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Page 2: Introduction
Introduction The Oxion series has been designed with all kind of Life Sciences applications and great durability in mind. This resulted in a modern, robust and high level microscope for everyday use, equipped with excellent optical and mechanical components. Specific attention to production methods resulted also in an excellent price/performance ratio. - Page 3 Phase contrast The observation of living cells and specimens with poor contrast is made possible by the use of the phase contrast method. Two different phase contrast equipments are available. All phase plan contrasts objectives can be used with both systems. A first economical way is to use sliders, each equipped with phase contrast rings.
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Page 4: Table Of Contents
Introduction ..........................2 Construction of the microscope ....................6 Preparing the Oxion microscope for use ..................7 Handling ......................................7 Assembling Steps ....................................8 Inserting/replacing the light source ..............................8 Mounting the objectives ..................................8 The microscope head ..................................8 Placing the eye pieces .................................. - Page 5 Using the Oxion range accessories ....................13 Using the Phase Contrast Slider ............................... 13 Mounting and operation of the Euromex Zernike phase contrast condenser set ................13 Using the Zernike phase contrast set............................... 14 Centering the phase rings ................................14 Using the Dark Field Slider ................................
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Page 6: Construction Of The Microscope
Construction of the microscope The names of the several parts are listed below and are indicated in the picture: Photo port Revolving nosepiece for 5 objectives Microscope head Objectives Set screw for tube Object clip Stand arm Object x/y stage Stage controls Condenser with iris diaphragm Coaxial fine adjustment... -
Page 7: Preparing The Oxion Microscope For Use
Preparing the Oxion microscope for use Handling Your microscope is a delicate product, please handle it with care. Carefully remove the items from their packing and place them on a flat, firm surface. Please do not expose the microscope to direct sun light, high temperatures, damp, dust or acute shake. Please make sure the worktable is flat and horizontal. -
Page 8: Assembling Steps
Assembling Steps Euromex Microscopes will always try to keep the number of assembly steps for their customers as low as possible but in some cases there are some steps to be taken. The steps mentioned below are often not necessary but described for your convenience nonetheless. -
Page 9: Operation
Adjusting the focusing tension The Oxion series microscope focusing knobs can be adjusted for tension. You can set it from light to heavy according your own preference. Please note that when the specimen leaves the focus plane after focusing or the stage declines itself, the tension should be set higher. -
Page 10: The Interpupillary Distance
People wearing glasses When the eyeshades bother people wearing glasses, the Oxion series eyeshades simply can be folded back to create more space. -
Page 11: Abbe Condenser
Abbe condenser Beneath the object stage an Abbe condenser (M) N.A. 12.5 is mounted. The condenser can be adjusted in height by means of a rack and pinion movement and knob (H). With this one can focus the light on the specimen by which the contrast can be optimized. The condenser is factory pre- centered if needed the following procedure can be followed to center the condensor. -
Page 12: Use Of The S100X Oil-Immersion Objective
2. Focus the image with the S40x objective. 3. Turn the revolving nosepiece so the S100x objective almost reaches the click-stop. 4. Put a small drop of immersion oil on the centre of the slide (always use Euromex Immersion oil). -
Page 13: Using The Oxion Range Accessories
When observing phase contrast, please set the aperture diaphragm indicator to “PH” position. Note: the phase diaphragms in the sliders are pre-centered do not need to be adjusted in operation. Mounting and operation of the Euromex Zernike phase contrast condenser set Use of phase contrast with the Oxion microscope... -
Page 14: Using The Zernike Phase Contrast Set
Using the Zernike phase contrast set. Any Oxion model with a Zernike phase contrast set comes with the condensor and objectives already mounted and centered on your microscope. If you suspect misalignment or want to check the alignment please see the next point for ”centering the phase rings”. The height of condenser can be adjusted in height by means of a rack and pinion movement. -
Page 15: Using The Simple Polarizing Set
Using the simple polarizing set 1. Simple polarizing includes analyzer and polarizer 2. Remove the dust cover from the filter slot (M), and insert the analyzer text face up. 3. Put the polarizer into the condenser slot. 4. Rotate the polarizer to change the state of polarization. Using the 0.5x ½... -
Page 16: Maintenance And Cleaning
In case there is still dust visible please check if the dust is in the eyepiece by turning it. If this is the case remove the lowest lens carefully from the eyepiece and clean it. Euromex strongly recommends using Euromex cleaning accessories for your Oxion microscope It is not necessary –... -
Page 17: Accessories
Accessories Eyepieces and eyeshades AE.3210 HWF 10x/22 eyepiece AE.3215 HWF 15x/13 eyepiece AE.3223 HWF 10x/22 eyepiece with micrometer reticule AE.3225 Pair of eyeshades Objectives AE.3104 Infinity Plan Achromatic 4x objective AE.3106 Infinity Plan Achromatic 10x objective AE.3108 Infinity Plan Achromatic 20x objective AE.3110 Infinity Plan Achromatic S40x objective AE.3112 Infinity Plan Achromatic S60x objective AE.3114 Infinity Plan Achromatic S100x objective... -
Page 18: Sliders And Phase Contrast Sets
Sliders and phase contrast sets AE.3183 Phase contrast Zernike condenser with PLPH 10x, S40x, S100x oil and darkfield stop AE.3184 Phase contrast Zernike condenser with PLPH 10x, 20x, S40x, S100x oil AE.3185 Slider with darkfield stop AE.3187 Slider with phase rings for 10x and S40x plan phase contrast objectives AE.3189 Slider with phase rings for 20x and S100x oil plan phase contrast objectives Miscellaneous AE.3190 Polarizer/Analyzer set...
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