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After electrophoresis
Once the tracking dye reaches the bottom of the gel, turn off the
power supply, disconnect the leads, and remove the safety lid,
using finger leverage between the lid and the top of the heat
exchanger. (Lift straight up to avoid bending the banana plugs.)
If coolant is circulating, stop the flow and disconnect the fittings
or tubing.
Pull out the upper buffer chamber assembly. Pour the buffer into a
sink. Install the assembly in the dual gel caster and then release
the sandwiches by turning and removing the cams.
Unscrew the clamps from the sandwiches and remove. Gently
loosen and then slide away both spacers. Use the Hoefer Wonder
Wedge Gel Plate Separation tool to separate the plates.
Carefully lift the glass plate with the gel attached. Handle the
gel with care to avoid damaging it. Invert the plate and
position the gel low over the staining tray. Pry one corner of the gel
away from the glass and allow it to drop into the tray,
or, if the gel is thick enough to handle, lift it and place it into the
tray. To avoid splashing, add staining or fixative solution to the
tray after the gel is transferred.
Clean the unit as described in the next section.
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