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Note: To help hold the gasket
against the upper buffer chamber,
dab a small amount of Gel Seal at
each end of the gasket only and then
install.
Important! A smooth fit between the
sandwich and gasket is essential to
a good seal.
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p18
Final assembly
Upper buffer chamber
Rinse both buffer chambers with water and distilled water
thoroughly before each use. Note: Before using the first time,
disassemble the unit and wash with a dilute solution of a labora-
tory detergent and rinse thoroughly first with water and then with
distilled water.
Clean away any gel adhering to the exterior of the gel sandwiches.
If running only one gel: Block the second upper buffer cham-
ber slot by installing the acrylic buffer dam included with the unit.
Fit clamps onto the dam, taking care to align the clamp ends and
dam edges. Install the "dummy" gel, screws facing out, in the
second cradle in the dual gel caster.
Attach the gel sandwich to the upper buffer chamber
Turn the upper buffer chamber upside down and place a slotted
gasket into both sandwich holder recesses. Both the slot in the
gasket and the slot in the recess must align. Both slotted gaskets
must be used even if running only one gel sandwich. Grooves along
each slot help keep the gasket in place.
Release the sandwiches from the caster by removing all bottom
cams (if present). Lower the upper buffer chamber onto the gel
sandwiches in the casting stand. Install the cams, ridge pointing
down, into the buffer chamber cam holes. Clamp the sandwich in
place by simultaneously turning one cam clockwise and the other
counterclockwise a full 180°.
Note: Do not force the cams. If you encounter unusual resistance,
disassemble and inspect clamp and glass alignment along the top of
the sandwich. Align and reinstall.
Use a pipette to carefully fill each slot above the sample wells with buf-
fer to minimize disturbing the samples. Then pour 100 ml of buffer
into the chamber, directing the buffer stream toward the side wall.
Check that no buffer leaks around the gasket.
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