BIOTIX COBRA PIPETTE Contents Page 1- INTRODUCTION 2- PARTS CHECK LIST 3- DESCRIPTION 4- SETTING THE VOLUME 5- USER ADJUSTMENT 6- PIPETTING 7- GENERAL GUIDELINES FOR GOOD PIPETTING 10 8- ACCESSORIES 9- GLP FEATURES 10- TROUBLESHOOTING 11- LEAK TEST 12- MAINTENANCE 13- CLEANING AND STERILIZATION 14- SPECIFICATIONS 15- SPARE PARTS...
USER’S USER’S GUIDE GUIDE 1 - INTRODUCTION The LabPRO pipette is an air displacement mechanism and is used with disposable pipette tips. This pipette line provides: • Light and comfortable body • Low pipetting forces ensuring ergonomics and users’ well-being •...
BIOTIX COBRA PIPETTE MECHANICAL PIPETTE ® 3 - DESCRIPTION Multichannel models Single models Color-coded push-button Lock/Unlock mecha- nism & Tip-ejector button Handle Counter Ejector clip Connecting nut Tip-holder Ejector support Ejector lock Cover Tip-ejector Tip-holders FIGURE 1 For 2µl and 10µl Single channel models, a tip-ejector extention is supplied to fit with short tips models.
USER’S USER’S GUIDE GUIDE To remove the tip-ejector extention: Gently twist the extention Pull it away from the pipette Ejector spacer for Multichannel models (X10µl only) Multichannel models fit with long collar tips. If you use short collar tips, you might have to insert the ejector spacer especially indicated for that: •...
USER’S USER’S GUIDE GUIDE To obtain maximum accuracy when setting the volume, proceed as follows: when decreasing the volume setting, slowly reach the > required setting, making sure not to overshoot the mark. when increasing the volume setting, pass the required >...
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BIOTIX COBRA PIPETTE Use the calibration key to adjust FIGURE 7 the pipette a. Insert the metal rod into the calibration key on the circle tip side (see figure 7). Lock it into place. You should feel the in- FIGURE 8 ternal part of the calibration key clamped and clipped on firmly.
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USER’S USER’S GUIDE GUIDE Pre-rinse the tips Some liquids (e.g.protein-containing solutions and or- ganic solvents) can leave a film of liquid on the inside wall of the tip; pre-rinse the tip to minimize any errors that may be related to this phenomenon. Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it to waste.
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BIOTIX COBRA PIPETTE You may wipe any drop lets away from the outside of the tip using a medical wipe, however if you do so take care to avoid touching the tip’s orifice. / For the multichannel models, use a reagent reservoir. Dispense Place the end of the tip against the inside wall of the ...
USER’S USER’S GUIDE GUIDE Each new tip should be pre-rinsed with the liquid to be pipetted. Liquid should never enter the tip-holder; to prevent this: • press and release the push-button slowly and smoothly, • never turn the pipette upside down, •...
BIOTIX COBRA PIPETTE To identify or personalize your pipette, identification clips are available: Identification clips (mixed colors set of 10) FE07060 Identification clips (red, set of 10) FE07061 Identification clips (yellow, set of 10) FE07062 Identification clips (green, set of 10) FE07063 Identification clips (blue, set of 10) FE07064...
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USER’S USER’S GUIDE GUIDE For single models Symptom Possible Cause Action Pipette is leaking sample Damaged tip-holder Replace the tip-holder Worn O-ring or seal Replace both parts and lubricate Pipette won’t aspirate Worn O-ring Replace both parts and lubricate Damaged tip-holder Replace the tip-holder Connecting nut is loose Tighten connecting nut...
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BIOTIX COBRA PIPETTE 11 - LEAK TEST This test may be performed at any FIGURE 14 time to check that the pipette does not leak, especially after perform- ing a maintenance or sterilization no leak procedure. If a pipette fails this test, replace the O-ring and seal.
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USER’S USER’S GUIDE GUIDE For the Multichannel models (8X - 12X): Fit Pipette tips. Set the pipette to the maximum volume given in the specifications, and pre-rinse. Aspirate the set volume from a reagent reservoir of distilled water. Maintain the pipette in the vertical position and wait ...
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BIOTIX COBRA PIPETTE Changing the tip-ejector FIGURE 15 To remove Push the ejection button. Push laterally (to the left) the tip-ejector. Slide and remove the tip-ejector. To refit Push the ejection button. Slide the tip-ejector along the ...
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USER’S USER’S GUIDE GUIDE Leave exposed the piston, clean it with isopropanol or ethanol and lubricate lightly. For 20µl, 100µl, 200µl Single channel models: lubricate only the useful part of the piston (20 ±5 mm length) and the O-ring. For 1000µl single channel model: lubricate the piston.
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USER’S USER’S GUIDE GUIDE 12B - MAINTENANCE FOR THE MULTI- CHANNEL MODELS ONLY Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance. Maintenance is limited to: • Cleaning or sterilization (see Chapter 13 - Cleaning and Sterilization) •...
BIOTIX COBRA PIPETTE Changing the tip-ejector To remove the tip-ejector, keep both ejector locks depressed. Pull the tip-ejector down. To refit the tip- ejector, gently re-insert the tip-ejector vertically into the rails of the ejector support. Pull lightly on the tip- ejector to check the position.
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USER’S USER’S GUIDE GUIDE Wipe the entire pipette with a soft-cloth or lint-free tissue impregnated with soap solution, to remove all dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used. Wipe the entire pipette and the tip-ejector with a soft ...
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BIOTIX COBRA PIPETTE Immerse the tip-ejector, and ejector spacer in the cleaning solution or wipe them with a soft cloth or lint- free tissue impregnated with the cleaning solution. Rinse the components with distilled water. Wipe the entire pipette with a soft cloth or lint-free ...
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USER’S USER’S GUIDE GUIDE Immerse tip-ejector, tip-holder and connecting nut in the cleaning solution. Degrease the piston assembly, the seals and im- merse them in another vessel. Rinse each component with distilled water. Leave the parts to dry by evaporation (or wipe with ...
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Checking and recalibrating your pipette with the tips that you use may be needed. Each pipette is inspected and validated by qualified technicians in accordance with DKSH Quality System. DKSH declares that its manufactured pipettes comply with the requirements of the ISO8655 standard, by type testing.
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USER’S USER’S GUIDE GUIDE The data given in the tables are achieved with Gilson standard length series pipette tips. If you are using other 10 µL extended length series pipette tips, you will need to recalibrate your pipette to comply with specifications. Multichannel models SPECIFICATIONS - Error limits Model...
BIOTIX COBRA PIPETTE MAINTENANCE & CALIBRATION DKSH recommends pipette calibration and maintenance at least once annually by an authorized service provider. Please contact DKSH directly. Please refer to the last page detailling local DKSH offices list.
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GUIDE GUIDE WARRANTY DKSH warrants this pipette against defects in material under normal use and service for a period of 36 months from the date of purchase. An ex- tended warranty is possible on request. It extends the standard warranty coverage for a maximum of 2 years on selected components.
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