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LS720, LS620, LS600, LS560, LS460 Microscopes
Startup Manual
with
Etaluma's Lumaview Software
LS720 (with Phase Contrast
LS560/LS460 (with Phase Contrast
Accessory, optional and ordered
Accessory and Manual XY Stage, both
separately)
optional and ordered separately)
LS620 (with Phase Contrast Accessory
and Manual XY Stage, both optional
and ordered separately)
IMPORTANT: This Manual describes how to set up LS Microscopes (Section I) and
start using Lumaview software (Section II). It is important that you follow this Guide.
Steps described for LS620 also apply to LS600.
For assistance, please call 760-298-2355 or email support@etaluma.com.
Etaluma, Inc.
www.etaluma.com
Lumaview™ is the trademark of Etaluma, Inc.
™2009-2018 All rights reserved.
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Table of Contents
Related Manuals for Etaluma LS720
Summary of Contents for Etaluma LS720
- Page 1 IMPORTANT: This Manual describes how to set up LS Microscopes (Section I) and start using Lumaview software (Section II). It is important that you follow this Guide. Steps described for LS620 also apply to LS600. For assistance, please call 760-298-2355 or email support@etaluma.com. Etaluma, Inc. www.etaluma.com Lumaview™...
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Page 2: Table Of Contents
I. Setting Up the LS Microscope A. Items Included With Each LS Microscope B. Optional Accessories (purchased separately) C. Recommended Computer Specifications D. Placing the LS Microscope E. Removing the Shipping Lock (LS720 only) F. Connecting the Cables G. LED Power H. Phase Contrast Brightfield Installing Objectives K. -
Page 3: Setting Up The Ls Microscope
This document is available for download at http://etaluma.com/products/downloads. Any steps specific for a particular LS model are noted in italics. I. Setting Up the LS Microscope A. Items Included With Each LS Microscope 1. LS720 USB communication cable • External power supply/cord with country-specific plug •... -
Page 4: Placing The Ls Microscope
3 mm hex wrench. WARNING: If packing the LS720 for shipment, you must install the red shipping lock before placing into the shipping carton! If not installed, the LS720 can be damaged! See Appendix A for instructions. -
Page 5: Phase Contrast
Phase Accessory arm. attachment b. Connect the free end of the Phase Accessory communication cable to the round port on the LS720 back panel (left side) labeled External Lamp Power Out. This allows the Phase Accessory to be controlled by Lumaview. -
Page 6: Installing Objectives
A small amount of dust will not affect imaging. However if dust is significant and it appears to affect quality of the live image or snapped images, contact Etaluma by email (support@etaluma.com) or telephone (760-298-2355) to discuss possible methods for cleaning. -
Page 7: Downloading And Installing Lumaview
Insert the standard USB-A end of the supplied USB cable into a USB port on your computer and the other square USB-B end into the square port on the LS720 left side or the square port labeled TO COMPUTER on the back of the LS620, LS560 and LS460. The same USB port on your computer should be used every time you connect to the LS Microscope. - Page 8 In this case, the focus will drop to the correct level within a few minutes. 4. LS720 only: Place the sample in the labware carrier and ensure it is seated flat with the upper left corner firmly pushed into the frame. Close the incubator and return to Lumaview to focus. The motorized Z axis will maintain focus level and autofocus can be used to adjust any change in focus over time.
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Page 9: Software: Getting Started With Lumaview
3. LS620, LS560, or LS460: Sample to be imaged is present on the LS Microscope stage. 4. LS620 or LS560: Fluorescence shroud is over sample if imaging involves fluorescence. 5. LS720: Red shipping lock MUST be off, power cable is plugged into a standard AC outlet, and fluorescence shroud is available if imaging involves fluorescence. -
Page 10: Initialization And Calibration (Ls720 Only)
Home position because it is under the microplate carrier frame. 2. If this is the first time the LS720 has been connected to the current computer, it is first necessary to do a Calibration. Once completed, calibration is not needed unless a different computer is connected or the XY position appears to be different from the expected position, e.g., center of microplate wells. -
Page 11: Instrument Setup
While the maximum is 1900 x 1900 pixels, the default is set to 1600 x 1600 pixels for LS720 and LS620, and 1200 x 1200 pixels for LS560 and LS460. Note: If larger Frame Sizes are used, there will be some darkening in the corners. If faster frame rates are needed, use smaller frame sizes, e.g., 800 x 800 pixels.) - Page 12 7. Select the time in seconds for each LED to be on before image snap. Recommended time for fluorescence is 0.6 seconds (do not go below this time). Check Auto Composite (LS720 and LS620 only) to have images in multiple channels automatically composited and saved in the Composites folder.
- Page 13 However, when Lumaview is closed, the dialog box positions will not be saved. 3. LS720 only: Select a desired XY location in a sample labware using one of two options in Manual XYZ: a.
- Page 14 Illumination (%) until a mid-gray tone is seen in the Live Image Window. F. Manual Focusing 1. LS720 only: a. In Manual XYZ, move the Focus slider up while watching the Live Image Window to detect the sample coming into focus. The Focus slider area becomes green whenever the Focus slider is active.
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Page 15: Snapping Images
Etaluma folder. 3. Images are captured and saved as seen by the camera, i.e., from the bottom. LS720 only: If desired, images can be flipped horizontally to show how they would appear from the top. To accomplish this, check “Horz flip image”... -
Page 16: Videos From Time-Lapse And Live Video
Several options are available for compiling videos from sets of time-lapse (see Section II.L.) and live video images: 1. Lumaquant software offered by Etaluma (see Lumaquant Startup Guide for compiling images into video). The Lumaquant Startup Guide can be downloaded from Etaluma’s website (www.etaluma.com/products/downloads/). -
Page 17: Setting Up Autofocus (Ls720 Only)
K. Setting Up Autofocus (LS720 only) 1. Use Manual Image and Manual XYZ to find a focused live image in a desired XY position as described in Section II.F. above. In Manual XYZ, click the Autofocus Setup button to open its dialog box. - Page 18 LS620, LS560, and LS460, the Protocol feature is utilized. Click the “P” icon in the Left Tool Bar. a. LS720 only. P icon is sixth from the top and there are five tabs. b. LS620 only. P icon is sixth from the top and there are three tabs.
- Page 19 (Section II.E above). When settings are satisfactory, click the Use Manual Settings button in the Image tab to upload them into the Protocol. 8. LS720 only. Click the fourth tab X Y Z to define the locations in the labware to be imaged. Click Enable Stage to adjust the settings.
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Page 20: Creating A Z-Map
d. Autofocus plus Z-Map. Check both Use Auto-Focus and Use Z-Map. Set Autofocus Parameters as described above in Step b, this Section. Follow steps described below in Section II.L. to create a Z- Map for each location to be imaged in Protocol. To use an existing Z-Map, select the file under Z- Map File Selection. -
Page 21: Tiling Across Locations
1. After entering all information and saving the Protocol, it is ready to be run. 2. LS720 only. The fifth tab is “Data Review” and will present the images as they are captured. Channels, including multiples if a Composite, can be selected as well as the Sequence number of a Time-Lapse. - Page 29 – –...
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