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Etaluma LS560 Startup Manual

Lumascopes with lumaview (for use with ls560, ls460, ls600, ls620, ls720)
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LS560/460 Startup Guide
LS560 & LS460 Microscopes (Lumascopes)
-- Startup Guide --
with
Lumaview (for use with LS560, LS460, LS600, LS620, LS720)
LS560 (with Phase Contrast Accessory
and Manual XY Stage, both optional
and ordered separately)
IMPORTANT: This Guide describes how to set up the LS560 and LS460 (Section I) and start using
Lumaview (Section II). It is important that you follow this Guide.
Steps described for LS560 apply to both LS560 and LS460 unless specified otherwise.
For assistance, please call 760-298-2355 or email support@etaluma.com.
Etaluma, Inc.
3129 Tiger Run Court, Suite 112
Carlsbad, CA 92010
www.etaluma.com
Lumascope™ and Lumaview™ are trademarks of Etaluma, Inc.
™2009-2017 Etaluma, Inc. All rights reserved.
1
2017.11.20

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Summary of Contents for Etaluma LS560

  • Page 1 Manual XY Stage, both optional and ordered separately) IMPORTANT: This Guide describes how to set up the LS560 and LS460 (Section I) and start using Lumaview (Section II). It is important that you follow this Guide. Steps described for LS560 apply to both LS560 and LS460 unless specified otherwise.

  • Page 2: Table Of Contents

    LS560/460 Startup Guide Table of Contents I. Hardware: Setup of LS560 (steps also apply to LS460 unless specified) A. Items Included With LS560 (and LS460) B. Optional Accessories C. Recommended Computer Specifications D. Placing the LS Microscope E. Phase Contrast F.

  • Page 3: Items Included With Ls560 (And Ls460)

    D. Placing the LS Microscope 1. Remove LS560 from the shipping box (save box and packing materials) and place on a lab bench or counter in a standard room environment. If the LS560 will be used in an incubator or hood, it is recommended that the initial setup be carried out in a room with ambient conditions before placement into the specialized environment.

  • Page 4: Brightfield

    1. The LS560 is controlled by the Lumaview software program. The latest Lumaview version is downloadable from Etaluma’s website and must be installed prior to connecting your computer for the first time to the LS560 (due to the need for two Drivers that are installed on the computer at the same time).

  • Page 5: Downloading And Installing Lumaview

    Alternatively, the same Lumaview Install_v(version#).zip file can be copied from the flash drive that comes with the LS560. Verify that this file is the latest version as posted on the Etaluma website. 2. Lumaview will not run on a computer with Lumaview 500/400 or Lumaview 600-Series installed. To uninstall either of these versions, use the procedure described in Appendix B.

  • Page 6: Live Cell Imaging With An Ls Microscope In An Incubator

    LS560 (see photo above). We highly recommend use of the same USB port on your computer every time you connect to the LS560. It is also best to connect the LS560 directly to the computer USB port rather than through a USB hub.

  • Page 7: Instrument Setup

    LS560/460 Startup Guide 1. Launch the Lumaview software from the desktop icon (or other chosen location) and allow the LS560 to be discovered. This may take a minute or so the first time. Two windows will open on your monitor: a.
  • Page 8 Utilities in the title bar and from the pull-down menu select Detect Hot Pixels to open its dialog box. Information on next steps is available in the Help section.) c. Pseudo color FL channels (LS560 only) results in live and snapped fluorescent images colored green according to signal intensity.

  • Page 9: Finding A Live Image

    1. Place sample on deck. Manual XY movement of SBS labware (microplate footprint) is facilitated by Etaluma’s Manual XY Stage (optional). For labware that is not SBS, use a labware holder such as the Multi-Slide Holder for 1-4 microscope slides.

  • Page 10: Focusing

    There is no automatic compositing function in Lumaview for LS560 and LS460. To generate composite images of green fluorescence with phase contrast or brightfield captured on an LS560 or LS460, use an image analysis program such as Lumaquant, Fiji/Image J, or similar program.

  • Page 11: Time-Lapse

    LS560/460 Startup Guide 1. Lumaquant software offered by Etaluma (see Lumaquant Startup Guide for compiling images into video, 2. Your own compiling software such as ImageJ, Fiji, MSMoviemaker, or iMovie, or 3. Use of two freeware software programs together, i.e., AviSynth and MeGUI (instructions described in Appendix C).

  • Page 12: Live Video

    LS560/460 Startup Guide representative sample (Section II.D above). When settings are satisfactory, click the Use Manual Settings button in the Image tab to upload them into the Protocol. 5. To run the Protocol, click Run Protocol in the lower left of the Protocol window.

  • Page 13: Lumaview File Tree Organization

    1. It is first necessary to download and install the following two freeware programs. a. AviSynth video post-production program found on the AviSynth Wiki at http://avisynth.nl/index.php/Main_Page. Download and install the Official Build. The default save location for the program will be the Etaluma folder inside the Program Files (x86) folder. 2017.11.20...
  • Page 14 LS560/460 Startup Guide b. MeGUI video conversion program found at http://sourceforge.net/projects/megui/. After the zipped folder (MeGUI_2525_x86.zip) has downloaded, click to open and Extract All. Depending on your computer and Windows version, it may be necessary to right click on the .zip file to complete the extraction.

This manual is also suitable for:

Ls460

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