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Etaluma LS620 Operator's Manual

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LS620, LS560, LS460 Microscopes
Operator's Manual
With
Etaluma's Lumaview Software
LS560/LS460 (with Phase Contrast
LS620 (with Phase Contrast Accessory
Accessory and Manual XY Stage, both
and Manual XY Stage, both optional
optional and ordered separately)
and ordered separately)
For assistance, please call 760-298-2355 or email support@etaluma.com.
Etaluma, Inc.
Carlsbad, California
www.etaluma.com
Lumaview™ is the trademark of Etaluma, Inc.
™2009-2021 All rights reserved.

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Summary of Contents for Etaluma LS620

  • Page 1 LS620 (with Phase Contrast Accessory Accessory and Manual XY Stage, both and Manual XY Stage, both optional optional and ordered separately) and ordered separately) For assistance, please call 760-298-2355 or email support@etaluma.com. Etaluma, Inc. Carlsbad, California www.etaluma.com Lumaview™ is the trademark of Etaluma, Inc.

  • Page 2: Table Of Contents

    Table of Contents A. Items Included With Each LS Microscope I. Setting Up the LS Microscope B. Optional Accessories (purchased separately) C. Recommended Computer Specifications D. Connecting the Cables E. Phase Contrast F. Brightfield G. Installing Objectives H. About Lumaview Downloading and Installing Lumaview Connecting the LS Microscope Starting Conditions...

  • Page 3: Items Included With Each Ls Microscope

    1. Wait to connect the USB cable from the LS Microscope to the computer until Lumaview is 2. (LS620 and LS560 only). For almost all fluorescence imaging applications, power from the computer via the USB cable to the LS Microscope will be sufficient. If higher levels of illumination are required, e.g., with very dim samples or for photo bleaching(FRAP), insert the...

  • Page 4: Phase Contrast

    small round plug of the external power supply/cord into the round port labeled SUPPLEMENTAL POWER on the back of the LS Microscope and the electrical plug into an AC outlet. You should see an increase in the Illumination level beyond the 40% limit as indicated on the Illumination slider in Lumaview with USB only.

  • Page 5: Brightfield

    A small amount of dust will not affect imaging. However, if dust is significant and it appears to affect quality of the live image or snapped images, contact Etaluma by email (support@etaluma.com) to discuss possible methods for cleaning.

  • Page 6: Downloading And Installing Lumaview

    USB cable into a USB port on your computer and the other square USB-B end into the square port labeled TO COMPUTER on the back of the LS620, LS560 and LS460. It is important to connect the LS Microscope directly to the computer USB port and not use a USB hub.

  • Page 7: Software: Getting Started With Lumaview

    Image Window as well. SOFTWARE: GETTING STARTED WITH LUMAVIEW The following sections give instructions for getting started with Lumaview on the LS620, LS560 and LS460 and are intended as a Startup Manual only. Not all options are given and not all features may be explained in any particular section.
  • Page 8 automated LS720 model and thus this will need to be changed to your respective model in the Configuration>Instrument Settings Menu seen below. Click OK, change the model selection and restart Lumaview. Two windows will open on your monitor: Live Image Window inside the larger Main Window.

  • Page 9: Instrument Setup

    Instrument Setup to open its dialog box. 2. Check the Lumaview version to make sure it is the most recently posted version on the Etaluma website (www.etaluma.com/products/downloads/). Ensure that the LS Microscope being used is checked. If it is necessary to change the LS Microscope model number that is checked, you will be prompted to restart the program.
  • Page 10 5. Click the Frame Size button to open its dialog box. Using the pull-down menu, select frame size desired (frame size will be the number times itself because it is square). Note: If larger Frame Sizes are used, there will be some darkening in the corners. If faster frame rates are needed, use smaller frame sizes, e.g., 800 x 800 pixels.) If the Frame Size is changed, there will be a prompt to restart Lumaview.
  • Page 11 0.6 seconds (do not go below this time). Check Auto Composite (LS720 and LS620 only) to have images in multiple channels automatically composited and saved in the Composites folder. Check Individual Channel Folders to have images from multiple channels in a Protocol saved in separate folders (e.g., for compiling time-lapse videos in single...

  • Page 12: Finding A Live Image

    Gain as low as possible. Increase Illumination gradually to the desired brightness. For dim samples, if Illumination is at maximum, increasing the Gain can be used to increase signal but it will also increase the background. LS620 and LS560: Illumination can also be increased by using the external power supply/cord.

  • Page 13: Focusing

    If Manual Image Autosave was not checked, a Save As dialog box will open after the image was snapped. Images will be saved in the Etaluma folder formed automatically whenever Lumaview is downloaded and installed.

  • Page 14: Live Video

    1:1 with the monitor resolution (pixel:pixel) and with its Title Bar showing the full path where it is located. • Composites including phase contrast or brightfield (LS620, LS560) Note: this is a hack  • In Instrument Setup, make sure Auto Composite is checked.
  • Page 15 • On the Information tab, if you enter User Notes, they will become part of the new Protocol. You can also enter User Notes for a previously saved Protocol in this tab. • Click on the Acquisition tab to select Time-Lapse Mode. Enter the Interval and Duration information.

  • Page 16: Run Protocol

    • Click on the Image tab to select the channels to be used in the Protocol and adjust their settings. If settings were previously determined in Manual Image, transfer the values into the current Protocol by clicking the “Use Manual Settings” button. The desired values can also be entered manually.

  • Page 17: Creating Videos

    Several options are available for compiling videos from sets of time-lapse and live video images: A) The optional Lumaquant software offered by Etaluma (see Lumaquant Startup Guide for compiling images into video). The Lumaquant Startup Guide can be downloaded from Etaluma’s website (www.etaluma.com/products/downloads/).
  • Page 18 Appendix A Windows Configuration Troubleshooting and Driver Conflicts (may require Administrator status) You may encounter installation error messages such as the following: Dotnet Service Pack 2 Upgrade: This error which will be presented as one of these two messages and can be solved by going to the Microsoft download page here: https://www.microsoft.com/en-us/download/details.aspx?id=21.
  • Page 19 In this case, it must be enabled in Windows features as seen below. Driver Conflicts Occasionally conflicts can occur between the drivers used by Lumaview and other Windows drivers, preventing communication between the LS Microscope and the connected computer. Often this occurs after a Windows Update or after installing another instrument software, especially camera-based instruments.
  • Page 20 Restarting Lumaview, unplugging and replugging in the USB cable, or restarting the PC and relaunching Lumaview may resolve the issue. If not, removing an interfering driver and updating it with the Lumascope driver may be required and is described below. Open the Device Manager.
  • Page 21 It may be necessary to unplug the LS Microscope (via the USB cable) from the computer and look for any names in the list where the name font color has turned a lighter gray. Reconnect the LS Microscope and verify that the one that turned lighter gray is now darker gray again. This is the driver that conflicts with the LS Microscope drivers.
  • Page 22 The next dialog box that appears will show the choices for the hardware devices. The Show compatible hardware box should be checked. Select 600-Series Lumascope and click Next. Windows should now install the LS Microscope driver over the conflicting driver. When installation is complete, disconnect the LS Microscope from the computer and then reconnect it.
  • Page 23 Appendix B How to Disinfect Lumascope Microscopes Etaluma’s microscopes are revolutionary in their ability to be operated within cell culture incubators, providing the most stable and robust long term live cell imaging. Placing the LS Microscope in the incubator often includes a disinfection step before installing. In this Tech Note, we discuss the methods recommended.
  • Page 24 Appendix C Setting up Time-Lapse in Windows10 Power Settings 1. Navigate to Settings (click on the Windows icon on the lower left of your screen and then the gear icon). Choose System, then Power & Sleep. On the right side column click on Additional Power Settings.

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Ls560Ls460

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