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Summary of Contents for Etaluma LS720
- Page 1 LS720 Microscope Operator’s Manual With Etaluma’s Lumaview Software For assistance, please call 760-298-2355 or email support@etaluma.com. Etaluma, Inc. Carlsbad, California www.etaluma.com Lumaview™ is the trademark of Etaluma, Inc. ™2009-2021 All rights reserved.
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Page 2: Table Of Contents
M. Editing a Z-Map N. Tiling Across Locations O. Live Video P. Run Protocol Q. Live Cell Imaging with LS720 Microscope in an Incubator III. Appendices Removing Red Shipping Lock for LS720 Resolving Lumaview Driver Conflicts Disinfecting the LS Microscope Before Placement into an Incubator... -
Page 3: Setting Up The Ls Microscope
This document is available for download at http://etaluma.com/products/downloads. I. SETTING UP THE LS720 MICROSCOPE A. Items Included With Each LS720 Microscope Phase Contrast Accessory (Olympus) ■ • USB communication cable • External power supply/cord with country-specific plug • Hex wrench (3 mm) for removing/replacing shipping lock •... -
Page 4: Unpacking The Ls Microscope
D. Unpacking the LS Microscope 1. Remove LS720 Microscope from the shipping box (save box and packing materials) and place on a sturdy bench or counter. 2. If the LS Microscope will be used in an incubator or environmental chamber, it is recommended that the initial setup be carried out on the bench under ambient conditions before placement into the specialized environment. -
Page 5: Connecting The Cables
F. Connecting the Cables 1. Connect the USB type B end to the LS720 at the rear left corner of the microscope. Wait to connect the type A USB cable from the Microscope to the computer until Lumaview is installed. -
Page 6: Installing Objectives
Etaluma by email (support@etaluma.com). Deck release lever J. About Lumaview 1. The LS720 is controlled by the Lumaview software program. The latest Lumaview version is downloadable from Etaluma’s website and must be installed prior to connecting your computer for the first time. -
Page 7: Downloading And Installing Lumaview
Double click on the .msi installer file to start installation. If a Windows warning box about an unrecognized App appears, click Run anyway. You will be asked about the location; note that the default is a new Etaluma folder inside the Program Files (x86) folder. During installation, a Device Driver Installation Wizard will open. -
Page 8: Starting Conditions
When Lumaview is open, the icon that resides in your computer dock appears as a document with an arrow. If the Left toolbar icons are too small, close Lumaview, right click the Lumaview shortcut icon on the desktop and select Properties. LS720 Microscope Manual 2021... -
Page 9: Initialization And Calibration
No image can be obtained at the Home position because it is under the microplate carrier frame. 2. If this is the first time the LS720 has been connected to the current computer, it is first necessary to do a Calibration. Once completed, calibration is not needed again unless a different computer is connected. - Page 10 Window to detect when the well outline can be seen and is in sharp focus. The Focus slider area becomes green whenever the Focus slider is active. Using the X/Y Move arrows (and Step size arrows if needed), move the LS720 Microscope Manual 2021...
- Page 11 Make sure the centered well is A1 by moving to the left and up to confirm it is the corner well using the X/Y Move arrows, and then re-center the A1 well. In Calibration, click Accept Calibration. The Calibration dialog box will now convert to the Manual XYZ dialog box. LS720 Microscope Manual 2021...
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Page 12: Instrument Setup
Instrument Setup to open its dialog box. 2. Check the Lumaview version to make sure it is the most recently posted version on the Etaluma website (www.etaluma.com/products/downloads/). Ensure that the correct model microscope being used is checked. If it is necessary to change the LS Microscope model number that is checked, you will be prompted to restart the program. - Page 13 Not checking Manual image autosave will result in the Save As dialog box opening every time the camera icon is clicked and the image has been snapped. In this case, the image file name desired must be entered before clicking Save. LS720 Microscope Manual 2021...
- Page 14 To have the live image and labware move in the same direction, check either or both Reverse X dir And Reverse Y dir. This feature is particularly helpful when moving across labware, e.g, going well to well in a microplate LS720 Microscope Manual 2021...
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Page 15: Focusing
(FOV) observable with the Zoom off. 2. If Manual image autosave was checked in Instrument Setup, images will be saved automatically using the time-based numbering system. If Manual image autosave was not checked, a Save As LS720 Microscope Manual 2021... - Page 16 Images will be saved in the Etaluma folder formed automatically whenever Lumaview is downloaded and installed. 3. Images are captured and saved as seen by the camera, i.e., from the bottom. If desired, images can be flipped horizontally to show how they would appear from the top.
- Page 17 2. Transfer the manually determined Focus Z level in Manual XYZ to the Center position field of Autofocus Setup. The Focus range will be set to a default value of 200 um. Change this to the recommended Minimum Range for each objective magnification according to the following table: LS720 Microscope Manual 2021...
- Page 18 Z levels obtained and use the approximate mean for the Center position. For the range, determine the spread between the highest and lowest expected Z values and make sure the Range contains those values comfortably. LS720 Microscope Manual 2021...
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Page 19: Editing A Z-Map
4. Review the Z-Map and make changes if desired. Click OK to return to Edit Z-Map. Click Cancel to keep the Z-Map value as it was before. Click on other locations to check and/or change their Z-map values. Be sure to click OK to save any changes before leaving Edit Z-Map. LS720 Microscope Manual 2021... - Page 20 ROI Map in the Protocol XYZ tab will look like this: D. Writing and Using Protocols 1. To configure an automated microscopy run, including time- lapse, the Protocol feature is utilized. Click the “P” icon in the Tool Bar. LS720 Microscope Manual 2021...
- Page 21 See Appendix D c. Live Video will record a video at each location using a single LED illumination d. Time-Lapse Video will take a short video at each location at the time lapse interval rather LS720 Microscope Manual 2021...
- Page 22 LS720 Microscope Manual 2021...
- Page 23 12. Images can be saved to individual folders for each location. Check “Save to folders named for wells”. If you prefer images to go to one folder, uncheck the box. In Instrument Setup you may also choose the setting that saves images into folders according to channel . LS720 Microscope Manual 2021...
- Page 24 Autofocus will occur in the brightfield channel and then remain at that level to capture the red channel image. If you want the red channel to also be focusing, select both the brightfield and red channels. LS720 Microscope Manual 2021...
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Page 25: Tiling Across Locations
To extend the user interface to microscope distance use a long HDMI for the monitor and wireless mouse and keyboard. Etaluma, Inc. | 3129 Tiger Run Court, Suite 218 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com... - Page 26 The Lumaquant Startup Guide can be downloaded from Etaluma’s website (www.etaluma.com/products/downloads/). B. Other video compiling software such as ImageJ, Fiji, Windows Video Editor, and iMovie. Etaluma, Inc. | 3129 Tiger Run Court, Suite 218 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 27 Fig 2. Installed shipping lock from top showing 2 set screws 3. The LS720 has a subdeck has 2 vertical alignment marks on the front edge of the plate carrier near the thumbscrew and the top deck front edge. In Lumaview, Manual XYZ, set Z step size to 10mm, move plate carrier toward front until end of travel.
- Page 28 If plate carrier can be lifted at front, tighten the two 1-inch screws and then the 4 set screws a little more until plate carrier is securely locked. Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 29 Occasionally the upgrade will already be present but needs to be enabled and you will see this message when you try to upgrade. Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 30 Lumaview may resolve the issue. If not, removing an interfering driver and updating it with the Lumascope driver may be required and is described in next section. Open the Device Manager. Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 31 Root Hub or Standard Open HCD Host Controller). Examples of conflicting drivers identified thus far that might be there include: BodeVib, Holdtescs, Cypress, NDTech. Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 32 In the next dialog box that appears, click on Browse my computer for driver software. In the next dialog box that appears, select Let me pick from a list of device drivers Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com...
- Page 33 The next dialog box that appears will show the choices for the hardware devices. The Show compatible hardware box should be checked. Select 600-Series Lumascope and click Next. Windows should now install the LS Microscope driver over the conflicting driver. When installation is complete, disconnect the LS Microscope from the computer and then reconnect it.
- Page 34 Appendix C How to Disinfect Lumascope Microscopes Etaluma’s microscopes are revolutionary in their ability to be operated within cell culture incubators, providing the most stable and robust long term live cell imaging. Placing the LS Microscope in the incubator often includes a disinfection step before installing. In this Tech Note, we discuss the methods recommended.
- Page 35 Appendix D Setting up Time-Lapse in Windows10 Power Settings Navigate to Settings (click on the Windows icon on the lower left of your screen and then the gear icon). Choose System, then Power & Sleep. On the right side column click on Additional Power Settings.
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