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Etaluma LS820 Operator's Manual

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LS820 Microscope
Operator's Manual
With
Etaluma's LumaviewPro™ Software
Pre-Release Version 0.8
For assistance, please email support@etaluma.com.
Etaluma, Inc.
Carlsbad, California
www.etaluma.com
This document is available for download at http://etaluma.com/products/downloads.
Lumascope™ and LumaviewPro™ are trademarks of Etaluma, Inc.
2009-2023 All rights reserved

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Summary of Contents for Etaluma LS820

  • Page 1 LS820 Microscope Operator’s Manual With Etaluma’s LumaviewPro™ Software Pre-Release Version 0.8 For assistance, please email support@etaluma.com. Etaluma, Inc. Carlsbad, California www.etaluma.com This document is available for download at http://etaluma.com/products/downloads. Lumascope™ and LumaviewPro™ are trademarks of Etaluma, Inc. 2009-2023 All rights reserved...

  • Page 2: Setting Up The Microscope

    Setting up the microscope Items Included With Each LS820 Microscope AuraPhase Transmitted Illumination LED Fixture with cable Optional ambient light guard Fixture mounting knob LS820 Microscope Manual 2023...
  • Page 3 60 mm Petri dishes & Terasaki plates ■ ■ Microscope slides & 50 mm Petri dishes 4 microscope slides in parallel plastic ■ 4 microscope slides in parallel metal with magnetic clamps ■ Single slide and dish with adjustable clamping ■ LS820 Microscope Manual 2023...
  • Page 4 Connecting the Cables Connect the USB type C end to the LS820 at the rear left corner of the microscope. Wait to connect the type A USB cable from the Microscope to the computer until LumaviewPro is installed. Plug the external power supply/cord into the rear port and the plug into an AC outlet. An extension cord is provided in cases where the AC outlet is distant.
  • Page 5 Alternatively, the same LumaviewPro.zip file can be copied from the flash drive that comes with the microscope. It may be helpful to verify that this file is the latest version as posted on the Etaluma website. After installation, a LumaviewPro shortcut (orange logo icon) convenient for launching the software will be present on the desktop.
  • Page 6 Launching LumaviewPro opens the application as seen below: The side menus are accessed by clicking the blue arrows on the right and left edge of the application. Motion Control Panel Image Control Panel LS820 Microscope Manual 2023...
  • Page 7 LED with an appropriate lens for uniform illumination. Phase Contrast is a small ring of LEDs that match the Ph1 objectives we supply from Evident. Extended Phase is a larger ring of LEDs that is currently under development to provide Darkfield. LS820 Microscope Manual 2023...
  • Page 8 LS820 Microscope Manual 2023...
  • Page 9 Autofocus failures can be due to the incorrect magnification objective in the Microscope Settings as well as a variety of image quality issues such as high background, thick samples, and very few edges from which to calculate the contrast. LS820 Microscope Manual 2023...
  • Page 10 Protocol To configure an automated microscopy run, including time- lapse, the Protocol feature is utilized. LS820 Microscope Manual 2023...
  • Page 11 Run One Scan can be clicked and the thumbnails of one interval inspected before continuing. Set the Interval and Duration of the Protocol, remembering to hit Enter after each. Click Save As and name the Protocol. LS820 Microscope Manual 2023...
  • Page 12 Live Cell Imaging with the LS820 Microscope in an Incubator Prior to imaging in an incubator, place the microscope inside for at least 6 hours and preferably overnight to allow thermal equilibration. Before transferring to the incubator, disinfect the microscope using any of the suggested procedures in Appendix A.

  • Page 13: Disinfecting The Microscope

    Further, it reaches all incubator walls, shelves, reservoirs, air plenums, sensors, and other interior components, and it leaves only small amounts of sterile water droplets as a residual. For questions, contact Technical Support at support@etaluma.com.
  • Page 14 Appendix B Setting up Time-Lapse in Windows10 Power Settings Navigate to Settings (click on the Windows icon on the lower left of your screen and then the gear icon). Choose System, then Power & Sleep. On the right side column click on Additional Power Settings.
  • Page 15 Appendix C Setting up Time-Lapse in Windows11 Power Settings Navigate to Settings (click on the Windows icon on the lower left of your screen and then the gear icon). Choose System, then Power & Sleep. On the right side column click on Additional Power Settings.
  • Page 16 Appendix D Extending the Distance from Lumascope to PC Workstation Often the Lumascope is located in an incubator which does not allow the PC or laptop to be placed in a convenient place for operation within the 3 meters that the included USB cable permits. Warning! the USB cable included with the Lumascope cannot be substituted or extended! In order to locate the viewing and control of the Lumascope a further distance, the monitor, keyboard, and pointing device must be extended without moving the PC or laptop.