Related Manuals for Varian 900-LC Series
Summary of Contents for Varian 900-LC Series
- Page 1 Varian 900-LC Series Liquid Chromatograph Getting Started Manual Installation Category II Pollution Degree 2 Safety Class 1 (EN 61010-1) 8510243600 December 2007...
- Page 2 Varian 900-LC Series Liquid Chromatograph Varian, Inc. offices Varian, Inc. has offices in most countries. The major offices for liquid chromatography products are listed below: Varian Australia Pty Ltd (Manufacturing site) 679 Springvale Road Mulgrave, Victoria 3170 Australia International telephone: + 61 3 9560 7133...
- Page 3 Varian 900-LC Series Liquid Chromatograph Declaration of Conformity We hereby declare that the equipment listed below complies with the requirements of: The Low Voltage Directive 73/23/EEC (93/68/EEC) The EMC Directive 89/336/EEC (92/31/EEC and 93/68/EEC) Applicable Standards BS EN 61010-1:1993 BS EN 61326:2003...
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Page 5: Table Of Contents
Varian 900-LC Series Liquid Chromatograph Contents Safety Practices and Hazards..............9 General Electrical Hazards Compressed Gas Cylinders High Pressure Hazards Ultraviolet Radiation Flash Chromatography Solvent Hazards Ignition of Flammable Chemicals Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent Explosion of Vapor from Flammable Chemicals... - Page 6 1.3.6 Instrument Cooling Air Supply 1.3.7 Weights and Dimensions 1.4 Personal Computer Requirements Installation .................. 29 2.1 900-LC Series LC System to PC Communications 2.2 Installing the Galaxie™ Chromatography Data System 2.3 About the Varian Integrated HPLC Online Help 2.4 Hardware Installation 2.5 Installation Qualification Tests...
- Page 7 Varian 900-LC Series Liquid Chromatograph 3.6 Starting an Acquisition 3.6.1 Sequence 3.6.2 Quick Start 3.7 Printing a report 3.8 Saving Your Files Maintenance................55 4.1 Routine Maintenance 4.2 Cleaning 4.3 Spare Parts Troubleshooting ................59 Publication date: December 2007...
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Page 9: Safety Practices And Hazards
It is your responsibility to inform Varian representatives if the instrument has been used for the analysis of hazardous biological, radioactive, or toxic samples, prior to any instrument service being performed or when an instrument is being returned to the service center for repair, or is being recycled at end-of-life. -
Page 10: Electrical Hazards
Varian 900-LC Series Liquid Chromatograph Varian, this protection may be impaired. It is essential that no cover is bypassed, damaged or removed. The safety practices described below are provided to help the user operate the instrument safely. Read each safety topic thoroughly... -
Page 11: Compressed Gas Cylinders
Compressed Gas Cylinders Unless your system is equipped with a helium sparging unit or an ELS detector, there are no gas requirements for the 900-LC Series LC systems. For gas requirements on these devices, consult the Operation Manual or other documentation enclosed with the particular device. -
Page 12: High Pressure Hazards
• Use only approved regulators and connections. • Use only connector tubing that is chromatographically clean (Varian part number 0391832600) and has a pressure rating significantly greater than the highest outlet pressure from the regulator. Storage Cylinder Instructions Cylinders containing gas under pressure should be firmly secured to a rigid structure, and the storage area must be adequately ventilated. -
Page 13: Ultraviolet Radiation
Varian 900-LC Series Liquid Chromatograph Ultraviolet Radiation 900-LC Series Liquid Chromatograph systems that use an ultraviolet light source have shielding to prevent radiation exposure to personnel. For continued protection: • Ensure that any protective lamp covers are in place during operation. -
Page 14: Ignition Of Flammable Chemicals
Varian 900-LC Series Liquid Chromatograph Ignition of Flammable Chemicals This instrument is not explosion-proof. In unattended operation, do not use organic solvents having an ignition point below 70 °C. Beware of ignition hazard when using flammable chemicals such as organic solvents. -
Page 15: Other Precautions
Ensure that you have the correct solvent before connecting it to the instrument. Other Precautions Use of the 900-LC Series Liquid Chromatograph and accessories may involve materials, solvents and solutions, which are flammable, corrosive, toxic or otherwise hazardous. Careless, improper or unskilled use of such materials, solvents and solutions... -
Page 16: Warning Symbols
Warning Symbols The following is a list of symbols that appear in conjunction with warnings in this manual or on the 900-LC Series Liquid Chromatograph. The hazard they describe is also shown. A triangular symbol indicates a warning. The meanings of the... -
Page 17: Color Coding
When attached to the rear of the product, indicates that the product has been certified (evaluated) to CSA 1010.1 and UL 3101-1. Color Coding The various indicator lights appearing on Varian instruments and associated accessories are color coded to represent the status of the instrument or accessory. •... -
Page 18: Spurious And Harmonic Radiation Compliance
Varian 900-LC Series Liquid Chromatograph Spurious and Harmonic Radiation Compliance Your Varian 900-LC Series Liquid Chromatograph complies with the requirements of FCC rules and Regulations part 18, subpart H—Miscellaneous Equipment, at the date of manufacture. CE Compliance Your Varian 900-LC Series Liquid Chromatograph has been... -
Page 19: Introduction
LC system can be installed. You should keep the pre- installation manual for future reference. If you have misplaced your copy, you can obtain a free replacement from your local Varian office. Alternatively, download a PDF from the Varian, Inc. Web site, www.varianinc.com... -
Page 20: Notes And Hot Tips
Varian 900-LC Series Liquid Chromatograph • Menus, menu options and field names (e.g. select Copy from the Edit menu) have been typed in bold. Bold is also used to signify the pushbuttons appearing throughout the software (e.g. click OK). •... -
Page 21: Electrical Power Supplies
(active, neutral, ground or two active and ground) and should be terminated at an appropriate connection receptacle that is within reach of the system power cable. 1.3.2.1 900-LC Series HPLC System Power Connections Power supply phase Single Power supply, current rating and... - Page 22 Table 3. Fuses ✒ Note For safety reasons, any other internal fuse or circuit breaker is not operator accessible, and should only be replaced by Varian-authorized personnel. Fuse information on the rear of the instrument is the most up to date.
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Page 23: Gas Supplies
The main gas supply requirement is nitrogen for supply to ELS Detector. Nitrogen from a cylinder or nitrogen generator may be used with Varian 900-LC Series Liquid Chromatograph ELS Detector. Helium may also be required to if you are using a helium sparging system to degas solvents. -
Page 24: Waste Container
1.3.5 Laboratory Conditions The area selected for the operation of a Varian LC system must be free from drafts, corrosive atmospheres and vibration. Sample preparation areas and materials storage facilities should be located in a separate room. -
Page 25: Weights And Dimensions
Varian 900-LC Series Liquid Chromatograph in applications demanding high usage of corrosive materials, an external cooling air supply system is provided. It is strongly recommended that the cooling air be supplied from an environmentally controlled area that is away from the instrument exhaust and any other area where corrosive materials are stored or used. -
Page 26: Personal Computer Requirements
Personal Computer Requirements The recommended and minimum PC specifications are listed in the following table. They can also be found on Varian’s Web site at www.varianinc.com. The minimum configuration represents the absolute minimum that is required to run the Galaxie™ software. - Page 27 Higher rated PC components can be substituted for those listed above e.g., processor type, amount of memory, screen size and resolution, operating system version etc. For the latest PC recommendations please see the Varian, Inc. website at www.varianinc.com Publication date: December 2007...
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Page 29: Installation
Varian 900-LC Series Liquid Chromatograph Installation The Varian 900-LC Series Liquid Chromatograph must be installed by a Varian-trained, Varian-qualified or Varian-authorized representative. You should have completed and returned the form in the pre-installation manual stating that you have prepared the laboratory in accordance with the requirements detailed in that manual. - Page 30 Varian 900-LC Series Liquid Chromatograph Figure 1. Single 900-LC Series LC System in standalone configuration Figure 2. Multiple 900-LC Series LC Systems in standalone configuration Up to four systems can be controlled by one Galaxie Acquisition Server. For a client server system there will need to be a PC with...
- Page 31 Varian 900-LC Series Liquid Chromatograph systems. Figure 3. Networked multiple 900-LC Series LC System configuration option 1 Figure 4. Networked multiple 900-LC Series LC System configuration option 2 You or your network administrator must provide any hardware, adapters, cables, and configuration and setup work required to...
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Page 32: Installing The Galaxie™ Chromatography Data System
Ethernet port on the PC(s) and/or company network. Installing the Galaxie™ Chromatography Data System Varian recommends that you purchase a PC as part of the Varian 900-LC Series Liquid Chromatograph package. The PC included in the package will come with the appropriate operating system as loaded by the PC supplier. - Page 33 Stand-alone Installation Information When using a stand-alone configuration the software for the main server, acquisition server and client are installed on one PC. This PC can then be used to control up to four 900-LC Series HPLC systems. 2.2.3 Galaxie Installation Procedure The 900-LC Series Driver installation has several steps: Install the Galaxie core software.
- Page 34 Installing the Galaxie Core Software To install the Galaxie™ core software: 1. Please see the Galaxie Chromatography Data System Installation Guide, p/n 03-914948-02 for installation instructions. ✒ The 900-LC Series HPLC Driver requires Galaxie™ software Note version 1.9 sp1. Publication date: December 2007...
- Page 35 The drivers work with the Galaxie core software to provide instrument control and data acquisition. Go to the next section, Installing the 900-LC Series HPLC Driver, if this section is not required. To install other LC or GC device drivers: Please see the Galaxie Chromatography Data System Installation Guide, p/n 03-914948-02 for installation instructions.
- Page 36 900-LC Series HPLC Driver. To install the 900-LC Series HPLC Help: Remove the driver CD and insert the 900-LC Series HPLC Help CD when instructed. Click OK. If you click Cancel, a message will appear confirming your choice not to install the Online Help.
- Page 37 To configure IMS access for individual profiles use Galaxie Configuration Manager. If Yes was clicked in step 2, click OK at the user profile changed notification. The 900-LC Series Setup Wizard window appears. Enter in the name of the system. ✒ Note Ensure you have typed in the correct system name.
- Page 38 Varian 900-LC Series Liquid Chromatograph reboot the LC system communications card. Click Close when the initialization is complete. ✒ If an IP address still does not appear, contact your IT department Note to check your network connections. The LC systems must be on...
- Page 39 Re-create the LC system. See page 36 for instructions. 2.2.6 Update Galaxie to Version 1.9 SP1 The 900-LC Series HPLC Driver requires Galaxie version 1.9 sp1 to run. The ordering information for the CD is part number 03-950000- 98 (Galaxie Software Service Pack 1 (SP1) CD).
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Page 40: About The Varian Integrated Hplc Online Help
Throughout this manual references are made to the Varian 900-LC Series LC online help or Galaxie™ Chromatography Data Systems help. The Varian LC system online help is provided on a separate CD to the Galaxie™ software and is installed on your PC during the Galaxie™ software installation. -
Page 41: Hardware Installation
Varian 900-LC Series Liquid Chromatograph Hardware Installation Your LC system should be ready to operate after the Varian representative has installed it. However, over time you may need to replace some hardware components such as detector lamps, autosampler needles, etc or may need to install a new device to your LC system. -
Page 42: Using The Online Help
Varian 900-LC Series Liquid Chromatograph Using the Online Help The online help is context sensitive. Pressing the F1 key on any screen in the device control section of the Galaxie™ software will bring up help that relates to that screen. -
Page 43: About Ims
Varian 900-LC Series Liquid Chromatograph does not have the right to run maintenance software an error message is displayed. 2.8.2 About IMS The software has the menu bar at the top which includes: • File • Open Audit trail •... - Page 44 Varian 900-LC Series Liquid Chromatograph 4. Click Help. 5. Click Varian Integrated Help. 6. Click the plus sign next to Instrument Maintenance Software in the online help table of contents. 7. Click Audit Trails. Publication date: December 2007...
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Page 45: Operation
Integrated HPLC Help. To access this information: Select the Windows® Start button and choose Programs > Galaxie™ > Help > Varian Integrated Help. When the Integrated HPLC Help appears, click the How to link to view the available step-by-step instructions. -
Page 46: Connecting To A System
Varian 900-LC Series Liquid Chromatograph Turn on the LC system. To start the Galaxie™ software, click the Windows® Start button and then choose Programs > Galaxie™ > Galaxie™. Enter in your login details and click OK. 3.2.1 Connecting to a System Depending on the configuration you purchased, you may have up to four LC systems connected to one PC. -
Page 47: Configuring The Device Settings And System Parameters
Turn the detector lamp(s) on. It is imperative that whenever the Varian detector lamp is turned Caution on, the pump should be running with a non-absorbing solvent flowing through the cell, with no air bubbles present. -
Page 48: Flows
For additional information on your system components please see the 900-LC Series HPLC Online Help. 3.4.1 Flows In Flows you can: •... -
Page 49: Sample Introduction
Varian 900-LC Series Liquid Chromatograph downloaded your method to the LC system. 3.4.4 Sample Introduction Sample introduction can occur by using an autosampler or manual injector if you are running an analytical or analytical to semi- preparative LC system or via a manual injector or pump if you are running a preparative scale LC system. -
Page 50: Creating A Method
Varian 900-LC Series Liquid Chromatograph • set the option to turn the lamps on as the detector(s) power up • select normalization for the PDA detector • synchronize with other modules can be selected to have the system wait for when a contact closure is to be used to start data acquisition •... -
Page 51: Publication Date: December
Varian 900-LC Series Liquid Chromatograph 5. Choose the devices needed for your run by selecting or deselecting the options in the Device Select window. 6. On each device window set your parameters. ✒ Note Your System Parameters will automatically appear in your method. -
Page 52: Starting An Acquisition
Varian 900-LC Series Liquid Chromatograph Starting an Acquisition There are two ways to start an acquisition. You can have multiple injections by creating a sequence or single injections using Quick Start. 3.6.1 Sequence From the File menu click New > New Sequence. -
Page 53: Printing A Report
Varian 900-LC Series Liquid Chromatograph Choose Quick Start. Select the Project name, System name and Method name and then click OK. Click on the button with the three dots next to the File prefix field to open up variable options for your run name. Double click the variables to be used in your run name or type in a name for your run and then click OK. -
Page 54: Saving Your Files
Varian 900-LC Series Liquid Chromatograph printer, unless you specify otherwise. You can specify your printer options in the Print Setup dialog box, accessible from the File menu. As the Print dialog is a standard Windows® dialog, you can obtain “What’s this” help on an item by clicking the question mark icon in the top right corner and then clicking the item of interest. -
Page 55: Maintenance
Varian 900-LC Series Liquid Chromatograph Maintenance This chapter includes information on how to access the Varian 900-LC Series HPLC system maintenance requirements that may be carried out by an operator. Any maintenance procedures not specifically mentioned should be carried out only by Varian-trained, Varian-qualified or Varian-authorized representatives. -
Page 56: Routine Maintenance
This section refers only to maintenance procedures for the instrument. You should refer to your PC and printer manuals for their maintenance procedures and to the 900-LC Series HPLC Help for the maintenance procedures for any accessories you ordered. Routine Maintenance Routine maintenance information is provided in the 900-LC Series HPLC online help. -
Page 57: Cleaning
Varian 900-LC Series Liquid Chromatograph Click Varian Integrated Help. Click the Maintenance link. Click Checklist. Cleaning Any spills in the sample compartment or on the LC system should be wiped up immediately. The exterior surfaces of the instrument should be kept clean. All cleaning should be done with a soft cloth. - Page 58 Varian 900-LC Series Liquid Chromatograph This page is intentionally left blank. Publication date: December 2007...
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Page 59: Troubleshooting
Varian 900-LC Series Liquid Chromatograph Troubleshooting For troubleshooting help please refer to the Varian 900-LC Series HPLC online help. To access the online help troubleshooting section: Click the Windows® Start button. Click Programs. Click Galaxie™. Click Help. Click Varian Integrated Help.