Exposure Settings - Nikon TE2000 Instructions Manual

Inverted microscope; colour image acquisition using nis-elements [basic research] software and the nikon digital sight ds-5m colour camera

Hide thumbs Also See for TE2000:

You can modify the live image by altering the DS-5M Settings options [image

acquisition parameters], e.g. exposure, sharpen and contrast.

Exposure settings

With exposure set to Manual, adjust the exposure time as required. The time is

listed in milliseconds to seconds. A slower exposure time will brighten the image.

You can change the exposure mode from Manual to Auto Exposure.

Adjust the image Contrast by using the drop down menu (e.g. Medium).

With manual exposure, typically set the Gain

exposure' can apply high levels of gain and introduce excessive image noise.

Checking the "Pixel Saturation Indication" [cntrl-shift-S] box will enable you to

see pixels that are saturated: over-exposed & overlaid bright red. Click the View,

Visualization Controls, LUTs

Using the colour camera, go to a clear area of the slide and click 'Auto White'

[DS-5M Settings]. This will change the colour balance to the correct setting so that

stained sections will appear with the correct colour and hue [e.g. white light will

be white – with no colour cast].

Use a consistent manual exposure time if you want to compare samples (or the

software might automatically brighten/darken inherently dark/light images). Also

ensure your light balance and other settings (e.g. Gain, Sharpen, Contrast) are

consistent [DS-5M Settings, Advanced]. Make a note of your camera settings –

these are automatically saved in Tiff and nd2 files, but not with jpg files.

Select the correct nosepiece objective

software using the Manual Microscope Pad [cntrl-alt-M]. Set the microscope

zoom to 1.0x or 1.5x using the

image in m/pixels allowing a scale bar to be added - using the Show Scale

button on the left of the live image window.

The lighter white colour scheme of the NISElements software can be changed to a

dark graphite/black colour if wished, using the sun/moon tab

of the screen.

Avoid increasing the camera gain as it will introduce excessive noise. With bright-field

images you can increase the halogen lamp brightness instead. Higher camera Gain can help

reduce sample bleaching with light sensitive fluorescence samples. You can also 'average

frames' to reduce noise using Acquire, Advanced Capture.

to 1.0. Be aware that 'Auto

button to enable/disable this function.

via NISElements

tab in the top menu. This will calibrate the

at the top right

Table of Contents