Leak Test; Cleaning And Decontamination; Autoclaving; Chemical Decontamination - Gilson MyPipetman User Manual

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Leak Test

This test may be performed at any time to check that the pipette does not leak, especially after performing
a maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal. After
making sure that the pipette is correctly reassembled, repeat this test.
1.
Fit a PIPETMAN DIAMOND Tip.
For the variable volume models, set the pipette to the maximum volume given in the specifications,
2.
and pre-rinse.
NOTE
For P2 to P200, pre-rinse the tip.
3.
Aspirate the set volume from a beaker of distilled water.
4.
Maintain the pipette in the vertical position and wait for
20 seconds.
If a water droplet appears at the end of the tip, there is a leak.
If you see no droplets, re-immerse the tip below the
surface of water.
The water level inside the tip should remain constant; if the level
goes down there is a leak.

CLEANING AND DECONTAMINATION

MyPIPETMAN pipettes are fully autoclavable without disassembly for maximum convenience as well as
protection from contamination.

Autoclaving

1.
Before autoclaving
Set the pipette volume to the lowest designated usable volume of the model before placing it in the autoclave.
2.
Procedure
MyPIPETMAN can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative pressure for
20 minutes without disassembly.
When autoclaving, the pipette will dry better and faster without using a bag.
3.
After autoclaving
Following the autoclaving cycle, let the pipette cool down to room temperature and dry completely before use.
Verify pipette's functionality according to your procedure.

Chemical Decontamination

You may choose to decontaminate your pipette chemically, in accordance with your own procedures.
Whatever decontaminant you use, check with the supplier to verify that the decontaminant is compatible
with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT
(Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate),
POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or
PPS (Polyphenylene Sulfide).
Chapter 6
MyPIPETMAN®
no leak
Figure 10
MyPIPETMAN® multichannel leak test
|
USER'S GUIDE
14

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