Nikon Eclipse TI-TIRF-E Setup Manual
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Nikon Eclipse TI-TIRF-E Setup Manual

Illuminator unit/motorized illuminator unit/photo activation illuminator unit, beamsplitter 50/50
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TI-TIRF Illuminator Unit
TI-TIRF-E Motorized Illuminator Unit
TI-PAU Photo Activation Illuminator Unit
TI-TIRF-PAU Illuminator
LU4-B5 Beamsplitter 50/50
<For Authorized Nikon Personnel>
WARNING
This manual is intended to provide setup instructions for Nikon
representatives who have attended the lecture on laser safety and have been
trained in setup operations. Attempts by others to set up the product may
lead to accidents or equipment failure. NIKON is not responsible for personal
injury or equipment damage resulting from equipment setup performed by
unqualified persons.
Be sure to observe the warnings and cautions described in this manual to
ensure safety during setup.
Series
Setup Manual
M456 E
10.9.NF.5
*M456EN05*

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Summary of Contents for Nikon Eclipse TI-TIRF-E

  • Page 1 Attempts by others to set up the product may lead to accidents or equipment failure. NIKON is not responsible for personal injury or equipment damage resulting from equipment setup performed by unqualified persons.
  • Page 3 • Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact a Nikon Service Department. • Some of the equipment described in this manual may not be included in the set you are installing.
  • Page 4 Safety Precautions To ensure correct and safe operation, read this manual before using the product. WARNING and CAUTION Symbols Used in This Manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct installation, read the setup manual carefully and thoroughly before installing the product.
  • Page 5 Safety Precautions Meaning of the Symbols Used on the Product The symbols marked on the product indicate the need for caution at all times during use. Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol is marked.
  • Page 6 Intended application of the product This product can be used only for microscopic observations. This product is required to be attached to the specific Nikon microscope, Ti-E, Ti-E/B, Ti-U, Ti-U/B. Do not use this product for other purpose. Setting up the product Setup of this product must be handled by trained service personnel.
  • Page 7 WARNING Prohibition of dismount This product uses a laser device. Use only in the configuration set up by the Nikon representative. To ensure safety, never attempt to remove any part of the system. Before beginning operations, confirm that all of the parts listed below are in place. Removal of any following part during use may result in unintended exposure of eyes or skin to laser light.
  • Page 8 Nikon products. However, the hazards described above could lead to an accident if you fail to heed all of the warnings and cautions in the manuals, if you strike the system, or if you attempt to disassemble the system.
  • Page 9 13. Notes on handling flammable solvent The following flammable solvents are used with the product: • Immersion oil (Nikon Immersion Oil for oil immersion objectives) • Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts) • Petroleum benzine (for wiping the immersion oil) •...
  • Page 10 If water enters a device, immediately suspend use of the product, unplug the power cord from the outlet, and contact your nearest Nikon representative. Do not place any object on the product.
  • Page 11 • Make sure that the product is not subject to strong vibrations, which may degrade image quality. Consult with Nikon representatives about preventive measures you may take when the product is installed. • To prevent degradation, malfunction, or failure of the laser and the PC, always use power supply that is free from electric noise or sudden voltage fluctuations.
  • Page 12 How to Use This Manual Configuration of This Manual This setup manual is made up of two parts. Refer to the part which deals with the product you are setting up. • Part 1 For Setting Up TI-TIRF, TI-TIRF-E, or TI-PAU •...
  • Page 13 Part 1 For Setting Up TI-TIRF, TI-TIRF-E, or TI-PAU Series Setup Manual <For Authorized Nikon Personnel>...

  • Page 15: Table Of Contents

    Contents System Configurations and Part Names.................... 1-4 1.1 Overview ............................1-4 1.1.1 TIRF Illuminator Unit and Ti-U System Overview .............. 1-4 1.1.2 Motorized TIRF Illuminator Unit and Ti-E System Overview ..........1-8 1.1.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System Overview..... 1-12 1.1.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System Overview......
  • Page 16 Contents Setting Up the Microscope ........................ 1-71 4.1 List of Parts ..........................1-71 4.2 Setup Procedure .......................... 1-74 4.2.1 Assembling Each Part and Attaching Them to the Microscope........1-74 4.2.2 Attaching the N-STORM Kit ..................... 1-85 4.2.3 Attaching the Laser Safety Kit..................1-87 4.2.4 Attaching the Stage Up Kit ....................
  • Page 17 Contents Connection between the Laser Unit and the Microscope ............1-213 6.1 Setting and Connecting the TI-LUSU Shutter Unit..............1-213 6.1.1 Control System Selection....................1-214 6.1.2 Control Mode of the Shutter Unit ................... 1-214 6.1.3 Setting and Connecting the TI-LU4SU Shutter Unit LU4..........1-215 6.1.4 DIP Switch Settings .......................

  • Page 18: System Configurations And Part Names

    System Configurations and Part Names Overview 1.1.1 TIRF Illuminator Unit and Ti-U System Overview TIRF illuminator unit and Ti-U system overview (front view): When used in combination with two-laser or three-laser unit (Standard configuration to use the TIRF illuminator unit) (12) (10) (11)
  • Page 19 Chapter 1 System Configurations and Part Names 1.1 Overview TIRF illuminator unit and Ti-U system overview (top view): When used in combination with two-laser or three-laser unit (10) (13) SER RADIATIO OID EXPOSU CLASS 3B LASE AVOID EXPOSURE LASER LIGHT IS EMITTE FROM OBJECTIVE APERT (12) (11)
  • Page 20 Chapter 1 System Configurations and Part Names 1.1 Overview TIRF illuminator unit and Ti-U system overview (front view): When used with four-laser module A (Standard configuration to use the TIRF illuminator unit) (13) (12) (11) (10) Figure 1.1-3 * For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.” (1) Power supply devices for the laser devices (2) Laser unit (LU4A) (3) PC...
  • Page 21 Chapter 1 System Configurations and Part Names 1.1 Overview TIRF illuminator unit and Ti-U system overview (top view): When used with four-laser module A (Standard configuration to use the TIRF illuminator unit) (12) (13) SER RADIATIO OID EXPOSU CLASS 3B LASE AVOID EXPOSURE LASER LIGHT IS EMITTE FROM OBJECTIVE APERT...

  • Page 22: Motorized Tirf Illuminator Unit And Ti-E System Overview

    Chapter 1 System Configurations and Part Names 1.1 Overview 1.1.2 Motorized TIRF Illuminator Unit and Ti-E System Overview Motorized TIRF illuminator unit and Ti-E system overview (front view): When used in combination with two-laser or three-laser unit (Standard configuration to use the motorized TIRF illuminator unit) (16) (11) (14)
  • Page 23 Chapter 1 System Configurations and Part Names 1.1 Overview Motorized TIRF illuminator unit and Ti-E system overview (top view): When used in combination with two-laser or three-laser unit (Standard configuration to use the motorized TIRF illuminator unit) (16) (15) (11) (10) (14) SER RADIATIO...
  • Page 24 Chapter 1 System Configurations and Part Names 1.1 Overview Motorized TIRF illuminator unit and Ti-E system overview (front view): When used with four-laser module A (Standard configuration to use the motorized TIRF illuminator unit) (15) (14) (10) (13) (15) (12) (11) Figure 1.1-7 * For details, see Section 8.1, “Overall Configuration,”...
  • Page 25 Chapter 1 System Configurations and Part Names 1.1 Overview Motorized TIRF illuminator unit and Ti-E system overview (top view): When used in combination with four-laser module A (Standard configuration to use the motorized TIRF illuminator unit) (14) (15) (10) (15) (11) TI-LU4SU LASER...

  • Page 26: Motorized Tirf Illuminator Unit, Lu4A, N-Storm, And Ti-E System Overview

    Chapter 1 System Configurations and Part Names 1.1 Overview 1.1.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System Overview Motorized TIRF illuminator unit, LU4A, N-STORM, and Ti-E system overview (front view) (Standard configuration to use the Motorized TIRF illuminator unit) (15) (14) (13)
  • Page 27 Chapter 1 System Configurations and Part Names 1.1 Overview Motorized TIRF illuminator unit, LU4A, N-STORM, and Ti-E system overview (top view) (Standard configuration to use the Motorized TIRF illuminator unit) (14) (22) (21) (23) (19) (15) (20) (10) (15) (11) TI-LU4SU LASER EMISSION...

  • Page 28: Ti-Pau Photo Activation Illuminator Unit And Ti-U System Overview

    Chapter 1 System Configurations and Part Names 1.1 Overview 1.1.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System Overview Photo activation illuminator unit and Ti-U (front view): When used in combination with two-laser or three-laser unit (Standard configuration to use the photo activation illuminator unit) (10) (11) (12)
  • Page 29 Chapter 1 System Configurations and Part Names 1.1 Overview Photo activation illuminator unit and Ti-U (top view): When used in combination with two-laser or three-laser unit (Standard configuration to use the photo activation illuminator unit) (10) (12) LASER RADIATION AVOID EXPOSURE TO BE CLASS 3B LASER PROD AVOID EXPOSURE LASER LIGHT IS EMITTED...
  • Page 30 Chapter 1 System Configurations and Part Names 1.1 Overview Photo activation illuminator unit and Ti-U (front view): When used with four-laser module A (Standard configuration to use the photo activation illuminator unit) (12) (11) (10) Figure 1.1-13 * For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.” (1) Power supply devices for the laser devices (2) Laser unit (LU4A) (3) PC...
  • Page 31 Chapter 1 System Configurations and Part Names 1.1 Overview Photo activation illuminator unit and Ti-U (top view): When used with four-laser module A (Standard configuration to use the photo activation illuminator unit) (12) (11) LASER RADIATION AVOID EXPOSURE TO BE CLASS 3B LASER PROD AVOID EXPOSURE LASER LIGHT IS EMITTED...

  • Page 32: Optical Paths Of Lasers

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers Optical Paths of Lasers 1.2.1 Laser Unit (2) (1) (11) (11) (12) (10) C-LU2 two-laser unit C-LU3 three-laser unit C-LU3EX three-laser unit EX LU4A four-laser module A Figure 1.2-1 (1) Laser mounting position L1 (7) Laser coupling mirror (See the table below.)

  • Page 33: Microscope Part (Tirf Illuminator Unit And Ti-U System)

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers 1.2.2 Microscope Part (TIRF Illuminator Unit and Ti-U System) TIRF illuminator unit and Ti-U system (top view) Laser light SER RADIATIO OID EXPOSU CLASS 3B LASE AVOID EXPOSURE LASER LIGHT IS EMITTE FROM OBJECTIVE APERT RS-232C...

  • Page 34: Microscope Part (Motorized Tirf Illuminator Unit And Ti-E System)

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers 1.2.3 Microscope Part (Motorized TIRF Illuminator Unit and Ti-E System) Motorized TIRF illuminator unit and Ti-E system (top view) Laser light SER RADIATIO SER RADIATIO OID EXPOSU OID EXPOSU CLASS 3B LASE CLASS 3B LASE AVOID EXPOSURE...
  • Page 35 Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers 1.2.4 Microscope Part (Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System) Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System (top view) Laser light TI-LU4SU LASER EMISSION POWER Figure 1.2-6 Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System (front left view)

  • Page 36: Microscope Part (Photo Activation Illuminator Unit And Ti-U System)

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers 1.2.5 Microscope Part (Photo Activation Illuminator Unit and Ti-U System) TI-PAU photo activation illuminator unit and Ti-U system (top view) Laser light LASER RADIATION AVOID EXPOSURE TO BE CLASS 3B LASER PROD AVOID EXPOSURE LASER LIGHT IS EMITTED...

  • Page 37: Safety Labels And Openings

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings Safety Labels and Openings The following shows the types and locations of the safety labels affixed to each unit picture, as well as the openings provided in the product. The labels to be affixed to the microscope are shipped with the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit, and the laser safety kit.

  • Page 38: Ti-U Microscope Body (With The Tirf Illuminator Unit)

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.1 Ti-U Microscope Body (with the TIRF Illuminator Unit) Be sure to affix each label to the positions indicated in the figure below. The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

  • Page 39: Ti-E Microscope Body (With The Motorized Tirf Illuminator Unit)

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.2 Ti-E Microscope Body (with the Motorized TIRF Illuminator Unit) Be sure to affix each label to the positions indicated in the figure below. The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

  • Page 40: Ti-U Microscope Body (With The Ti-Pau Photo Activation Illuminator Unit)

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.3 Ti-U Microscope Body (with the TI-PAU Photo Activation Illuminator Unit) Be sure to affix each label to the positions indicated in the figure below. The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

  • Page 41: C-Lu2 Two-Laser Unit

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.4 C-LU2 Two-laser Unit Make sure that the labels are affixed in place. (1) IEC warning label - (6) IEC caution label - laser hazard symbol Class 3B area CAUTION –...

  • Page 42: C-Lu3 Three-Laser Unit

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.5 C-LU3 Three-laser Unit Make sure that the labels are affixed in place. (1) IEC warning label - (6) IEC caution label - laser hazard symbol Class 3B area CAUTION –...

  • Page 43: C-Lu3Ex Three-Laser Unit Ex

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.6 C-LU3EX Three-laser Unit EX Make sure that the labels are affixed in place. (1) IEC warning label - (6) IEC caution label - laser hazard symbol Class 3B area CAUTION –...

  • Page 44: Lu4A Four-Laser Module A

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.7 LU4A Four-laser Module A Make sure that the labels are affixed in place. (1) IEC warning label - (12) IEC caution label - Class 4 laser hazard symbol area (12)* Figure 1.3-7...

  • Page 45: Lu-Lr Four-Laser Ps Rack

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.8 LU-LR Four-laser PS Rack Make sure that the label is affixed in place. (11) Caution label (Front view) (11) (Rear view) Figure 1.3-8 1-31...

  • Page 46: Ti-Lusu Shutter Unit

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.9 TI-LUSU Shutter Unit For the TIRF illuminator unit or the motorized TIRF illuminator unit When used in combination with two-laser or three-laser unit Make sure that the labels are affixed in place. (4) Safety standard label (10) TI-TIRF special label Exclusive use for TI - TIRF system...
  • Page 47 Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings For the photo activation illuminator unit When used in combination with two-laser or three-laser unit Make sure that the labels are affixed in place. (4) Safety standard label (10) TI-PAU special label Exclusive use for TI - PAU system Don't use this unit with other systems.

  • Page 48: Ti-Lu4Su Shutter Unit Lu4

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.10 TI-LU4SU Shutter Unit LU4 For the TIRF illuminator unit or the motorized TIRF illuminator unit When used with four-laser module A Make sure that the label is affixed in place. (4) Safety standard label T h i s d e v i c e c o m p l i e s w i t h P a r t 1 5 o f t h e F C C R u l e s .
  • Page 49 Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings For the photo activation illuminator unit When used with four-laser module A Make sure that the label is affixed in place. (4) Safety standard label T h i s d e v i c e c o m p l i e s w i t h P a r t 1 5 o f t h e F C C R u l e s . O p e r a t i o n i s s u b j e c t t o t h e f o l l o w i n g t w o c o n d i t i o n s : ( 1 ) t h i s d e v i c e m a y n o t c a u s e h a r m f u l i n t e r f e r e n c e ,...

  • Page 50: Ti-Tirf Tirf Illuminator Unit

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.11 TI-TIRF TIRF Illuminator Unit Make sure that the labels are affixed in place. This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

  • Page 51: Ti-Tirf-E Motorized Tirf Illuminator Unit - For Normal Microscopy

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.12 TI-TIRF-E Motorized TIRF Illuminator Unit ― For Normal Microscopy (Except N-STORM) Make sure that the labels are affixed in place. This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

  • Page 52: Ti-Tirf-E Motorized Tirf Illuminator Unit - For N-Storm Microscopy

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.13 TI-TIRF-E Motorized TIRF Illuminator Unit ― For N-STORM Microscopy Make sure that the labels are affixed in place. This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

  • Page 53: Ti-Pau Photo Activation Illuminator Unit

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels and Openings 1.3.14 TI-PAU Photo Activation Illuminator Unit Make sure that the labels are affixed in place. This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

  • Page 54: Name Of Each Part

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Name of Each Part 1.4.1 TIRF Illuminator Unit and Ti-U System When used with two-laser or three laser-unit Filter slider cover (part of laser safety kit) TIRF illuminator unit TI-FLC epi-fl filter turret To laser unit Laser safety cover...
  • Page 55 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-TIRF illuminator unit (6) Focal point adjustment knob (12) ND filter slider (7) Condenser lens locking screw (4) ND filter sliders (3) Laser position adjustment knob (14) Connector for the (for forward and backward HG fiber light adjustment)
  • Page 56 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety cover The laser safety cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open. Upper cover Laser safety cover Body...
  • Page 57 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-FLC epi-fl filter turret The epi-fl filter turret is used to select an excitation Filter cube port TI-FLC epi-fl (and its cover) filter turret method by rotating the turret to change filter cubes including a dichroic mirror.
  • Page 58 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Optical path selector knob (on the microscope body) The laser shutter does not open when the eyepiece route of the optical path (“EYE”) is selected. Ti-U Observation port 100% PORT dial Right port 100% (optical path selector knob)

  • Page 59: Motorized Tirf Illuminator Unit And Ti-E System

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part 1.4.2 Motorized TIRF Illuminator Unit and Ti-E System When used in combination with two-laser or three-laser unit Motorized TIRF illuminator unit Filter slider cover (part of laser safety kit) TI-FLC-E or TI-FLC-E/HQ motorized epi-fl filter turret, with Protection plate (part of laser safety kit)
  • Page 60 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-TIRF-E motorized illuminator unit and remote control pad (4) Focal point adjustment knob (10) ND filter sliders (5) Condenser lens locking screw (3) ND filter sliders (2) Laser position adjustment knob (for forward and backward (12) Connector for the HG fiber light...
  • Page 61 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety cover The laser safety cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open. Upper cover Laser safety cover Body...
  • Page 62 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-FLC-E motorized epi-fl filter turret or TI-FLC-E/HQ motor epi-fl filter turret The epi-fl filter turret is used to select an excitation TI-FLC-E motorized epi-fl method by rotating the turret to change filter cubes Filter cube port filter turret or TI-FLC-E/HQ (and its cover)

  • Page 63: Motorized Tirf Illuminator Unit, Lu4A, N-Storm And Ti-E System

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Optical path selector switch (on the microscope body) The laser shutter does not open when the nosepiece tube route of the optical path (“EYE”) is selected. Ti-E/B Ti-E Observation port 100% Observation port 100% L100 Left port 100%...
  • Page 64 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-TIRF-E motorized illuminator unit (5) Condenser lens locking screw (11) ND filter sliders (6) STORM slider (4) Focal point adjustment knob (3) ND filter sliders (2) Laser position adjustment knob (13) Connector for the (for forward and backward HG fiber light...
  • Page 65 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety cover The cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open. Upper cover Laser safety cover Body...
  • Page 66 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety kit The laser safety kit is composed of the filter slider Filter slider cover cover, protection plate, filter cube port cover retraining plate, and safety labels. Before using the product, check that the part shown in the left figure is attached.
  • Page 67 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Optical path selector switch The laser shutter does not open when the nosepiece tube route of the optical path (“EYE”) is selected. Ti-E Observation port 100% L100 Left port 100% Optical path selector switch Left port 80% and observation port 20% R100...

  • Page 68: Ti-Pau Photo Activation Illuminator Unit And Ti-U System

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part 1.4.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System When used in combination with two-laser or three-laser unit Specimen cover TI-FLC epi-fl filter turret Photo activation illuminator unit Interlock cable TI-LUSU shutter unit...
  • Page 69 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Photo Activation Illuminator Unit HG fiber light source Laser position adjustment knob adapter/lamphouse (for forward and backward adjustment) Auxiliary filter slider mounting part for epi illumination Laser focus Laser focus adjustment adjustment knob clamp screw...

  • Page 70: Laser Safety Kit

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety kit The laser safety kit is composed of the filter slider Filter slider cover cover, protection plate for nosepiece, filter cube port cover retaining plate, and safety labels, “(9) IEC aperture label 2”...
  • Page 71 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-LUSU shutter unit Eyepiece optical path Safety cover signal connector The shutter unit controls motorized shutters in the signal connector Laser unit connector laser unit. AC adapter Serial IF connector connector Use this unit in combination with the C-LU2, C-LU3 or C-LU3EX laser unit.
  • Page 72 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part 1.4.5 Laser Unit Two-laser Unit (C-LU2) (1) Laser mounting position L1 (2) Laser mounting position L2 (3) Shutter unit connector (4) ND filter slider (5) Single-mode fiber Figure 1.4-42 * For details of available lasers for each position of laser units, see Section 8.1.4, “Mountable Lasers”...
  • Page 73 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Three-laser unit EX (C-LU3EX) (1) Laser mounting position L1 (2) Laser mounting position L2 (3) Laser mounting position L3 (4) Shutter unit connector (5) Single-mode fiber (6) Brightness control knob (7) Ar wavelength selector (optional) (8) AOM connector (optional) Figure 1.4-44...
  • Page 74 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Four-laser module A (LU4A) (1) Laser mounting position L1 (2) Laser mounting position L2 (3) Laser mounting position L3 (4) Laser mounting position L4 (5) Single-mode fiber ND filter sliders (7) Power switch (8) AC inlet (9) Remote switch...
  • Page 75 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Four-laser PS rack (LU-LR) (1) EMISSION laser indicator (2) POWER laser indicator (3) EMISSION key switch (4) Power switch (5) INTERLOCK connector (6) Cooling fan (7) Power breaker for 15 A line (8) Power breaker for 5 A line (9) Caution label (Front view)
  • Page 76 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part 1.4.6 Laser Safety Kit The laser safety kit is supplied with all laser products mounted onto a Ti microscope. Labels affixed to each laser product include a name label and other types of necessary labels, both of which are not shown here.
  • Page 77 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Protection plate (for nosepiece) The protection plate (for nosepiece) is a component designed for shielding the gap between the nosepiece and the epi-fl filter turret to avoid accidental jamming of fingers inserted into the gap or the laser radiation from the gap.

  • Page 78: Related Product

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part 1.4.7 Related Product TI-BPU back port unit Adding a camera port by attaching the TI-BPU back port unit to the microscope base allows for a concurrent image capture with two cameras. Attach a C mount camera via a separate C mount adapter to the back port unit and perform focus adjustment and centering.
  • Page 79 TIRF illuminator unit/photo activation illuminator unit to correct the condensing position and provide the optimum illumination performance. Contact your own Nikon representative for details on the stage up lens. The following figure shows the Ti-U with the stage up kit mounted, along with the TIRF illuminator unit on the upper tier and the back port unit on the lower tier.
  • Page 80 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Positioning pin Stage support block Nosepiece support block Epi-fl filter turret Shielding plate Epi-fl filter turret support block (left) support block (right) Positioning pin Dia-illuminator pillar support block Support pillar extension TI-BSUK70 70 mm stage up kit Figure 1.4-53...

  • Page 81: Preparations

    Preparations Check Items and Tools 2.1.1 Required Items Make sure you have all of the following items before starting. • PC (complete set) • PC-related devices • Operating system • Laser: Recommended laser (see Chapter 1, “System Configuration and Part Names”) •...

  • Page 82: Tools

    • Coupler-centering tool • Centering tool for visual observations and mirror for observing centering tool Figure 2.1-5 Figure 2.1-6 • Nikon 2-piece M4 screwdriver set • Polarization orientation adjustment tool and (with red handles) polarizer-equipped tool Figure 2.1-7 Figure 2.1-8 •...

  • Page 83: Overview Of Setup Procedure

    Chapter 2 Preparations 2.2 Overview of Setup Procedure Overview of Setup Procedure Setting Up the PC and Operation Software (Chapter 3) • Attaching the light source (TI-TIRF, TI-TIRF-E, or TI-PAU) Setting Up the Microscope • Attaching the dichroic mirror (TI-PAU only) (Chapter 4) •...

  • Page 84: Setting Up The Pc And Operation Software

    Setting Up the PC and Operation Software Using TIRF and PAU microscopy, images are observed on a monitor, via a camera attached to the microscope camera port. TIRF images and laser port images from PAU microscopy cannot be observed through the eyepieces. Make sure to set up the PC and operation software for the camera to be used. 1-70...

  • Page 85: Setting Up The Microscope

    Setting Up the Microscope List of Parts The parts below have been added to the basic set of the Ti-E, Ti-E/B, Ti-U, and Ti-U/B. The procedures for attaching sub-units and parts required for installing the TIRF-PAU illuminator are described later in this section. TIRF illuminator unit or motorized TIRF illuminator unit Table 4.1-1 Unit...

  • Page 86: 4.1 List Of Parts

    Chapter 4 Setting Up the Microscope 4.1 List of Parts Unit Name of equipment Model Remarks Commercially- available PC TIRF image Commercially- Monitor observation available monitor Commercially- An adapter is required for TV camera available TV camera connection to the microscope port. Two-laser unit C-LU2 See “Chapter 5 Laser Unit”...
  • Page 87 Chapter 4 Setting Up the Microscope 4.1 List of Parts Photo activation illuminator unit Table 4.1-2 Unit Name of equipment Model Remarks Ti-E Ti-E/B Use with the TI-DH dia-illuminator 100W. Ti-U Ti-U/B TI-LUSU Shutter Unit PC compatible TI-LU4SU AD-1260B AC adapter For TI-LUSU shutter unit EA1050E-120 TI-FLC...

  • Page 88: Setup Procedure

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Setup Procedure 4.2.1 Assembling Each Part and Attaching Them to the Microscope Perform the steps below to assemble each part and attach them to the microscope. Attach the light source (TIRF illuminator unit, motorized TIRF illuminator unit, or photo ⇒...
  • Page 89 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the light source (TIRF illuminator unit, motorized TIRF illuminator unit, or photo activation illuminator unit). Remove the black cover on the center of the microscope base. (1) Using an Allen wrench, remove the two screws securing the black cover.
  • Page 90 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Connect the mercury episcopic light source Mercury episcopic light source adapter adapter (or mercury lamphouse) to the episcopic light-source port (bayonet ring) Connect the episcopic light source on the side where you attached the support pillar in step 3. For details about attaching the light source, see the instructions of your mercury lamp power supply or super-high pressure mercury lamp...
  • Page 91 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Remove the anchoring bolt from the optical path Anchoring bolt switching module. The optical path switching module comes equipped with an anchor for transportation. Be sure to remove the anchor before use. Unscrew the anchoring bolt from the top of the optical path switching module.
  • Page 92 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Remove the mirror unit from inside the Holder plate locking screws illuminator, and insert the dichroic mirror. (1) When removing the mirror unit, pull out the mirror switch lever, and then pull out the mirror unit while holding the tab of the mirror unit (magnetic lock).
  • Page 93 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the epi-fl filter turret. Attach the epi-fl filter turret to the microscope main body. Insert the epi-fl filter turret along the attachment groove from the right side of the microscope and secure with screws on the left and right.
  • Page 94 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the laser safety cover (TIRF illuminator unit or motorized TIRF illuminator unit only). If you are using the TIRF illuminator unit or the motorized TIRF illuminator unit, the motorized shutter will not open unless the laser safety cover is attached on the stage.
  • Page 95 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the filter cubes. You can attach up to six filter cubes in the epi-fl filter turret. Remove the filter cube port cover, check the turret Indication of address address, and insert the filter cubes along the guide on the optical path (position for ID sticker) groove.
  • Page 96 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Affix the labels. After confirming the safety of the laser (p.1-228 to 1-230), affix the specified warning labels to the locations shown below. Left side of microscope main unit Affix “(7) IEC caution label - Class 3B area” on the left side of the microscope main unit, under the field diaphragm.
  • Page 97 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure On the laser safety cover (TIRF illuminator unit or motorized TIRF illuminator unit) Make sure that the following three warning labels at the rear left, when viewed from the Binocular eyepiece tube: “(1) IEC warning label - laser hazard symbol”, “(2) IEC explanatory label - Class 3B laser product”, and “(8) IEC aperture label 1”...
  • Page 98 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Laser unit Remove the laser safety label if it is attached to the laser unit. Remove the laser safety labels. About 12 mm About 20 mm About 12 mm About 30 mm Figure 4.2-19 1-84...

  • Page 99: Attaching The N-Storm Kit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.2 Attaching the N-STORM Kit Install the following parts to the microscope to perform N-STORM microscopy. Ti-E fixing plates Attach plates to fix the microscope to the anti-vibration table. Detach the front and rear rubber feet at the bottom of the microscope and secure the fixing plates (front and rear).
  • Page 100 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure DSC support columns (2 pieces) Attach supports to fix the camera to the anti-vibration table. Screw the supports in the screw holes in the camera (Andor iXon) body. Secure the camera to the side 3D-STORM port of the microscope and set the magnet base to ON.

  • Page 101: Attaching The Laser Safety Kit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.3 Attaching the Laser Safety Kit Refer to the instruction manual included with the microscope main body before attaching the laser safety kit. Attach the filter slider cover to the dia pillar Filter slider cover illuminator 100W.
  • Page 102 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure If the Perfect Focus System is used Remove the four M3 hexagon socket head bolts secured on top of the epi-fl filter turret M5 Hexagon TI-ND6-PFS socket head (motorized PFS (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ), using bolts (x 2) 6 nosepiece) the 2-mm hexagonal screwdriver appended...

  • Page 103: Attaching The Stage Up Kit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.4 Attaching the Stage Up Kit By attaching the TI-BSUK70 70 mm stage up kit and doubly mounting the epi-fl filter turrets, you can attach any of the TIRF illuminator unit/ motorized TIRF illuminator unit/photo activation illuminator unit and TI-BPU back port unit simultaneously.
  • Page 104 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Hexagon socket head bolt Hexagon socket head bolt (M5, two) (M5, four) Shielding plate Hexagon socket head bolt (M5, two) Stage support block Dia-illuminator pillar support Hexagon socket head button bolt block (M3, four) Epi-fl filter turret support block...
  • Page 105 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attaching the support pillar extension To attach the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit to the upper tier, attach the support pillar extension supplied with the stage up kit to the support pillar supplied with the illuminator unit.

  • Page 106: Attaching The Back Port Unit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.5 Attaching the Back Port Unit Insert the back port unit into the guide on the rear of the microscope body and use the hexagonal screwdriver (4-mm) to secure it from behind by tightening screws. •...

  • Page 107: Laser Unit

    Laser Unit WARNING • The unit must be set up as instructed by the local LSO (Laser Safety Officer). • This laser unit uses Class 3B lasers. Accidental exposure of eyes or skin to laser light may result in injury or other problems. Do not turn on the lasers until they are installed in the laser unit •...

  • Page 108: Overview Of The Laser Units

    Chapter 5 Laser Unit 5.1 Overview of the Laser Units Overview of the Laser Units A laser unit is a base device to mount laser devices. It introduces laser beam to the illuminator unit (the TIRF illuminator unit, the motorized TIRF illuminator unit, or the photo activation illuminator unit) through an optical fiber.
  • Page 109 Chapter 5 Laser Unit 5.1 Overview of the Laser Units C-LU2 two-laser unit This unit can mount two lasers. To control the laser intensity, insert or remove the two ND filters in front of each laser head. A motorized shutter is provided for each laser and can be controlled on the TI-LUSU shutter unit.
  • Page 110 Chapter 5 Laser Unit 5.1 Overview of the Laser Units Mountable position C-LU3EX Device C-LU2 two-laser C-LU3 three-laser LU4A four-laser three-laser unit unit unit module A Manufacturer: Spectra Physics 163 series (163-C02) 457 to 514 nm (40 mW) Manufacturer: Spectra Physics 163 series (163-C12) 488 nm (25 mW) Manufacturer: Spectra Physics...
  • Page 111 Chapter 5 Laser Unit 5.1 Overview of the Laser Units Mountable position C-LU3EX Device C-LU2 two-laser C-LU3 three-laser LU4A four-laser three-laser unit unit unit module A L1 (h) Manufacturer: Crystalaser * DL647-100 — — — (Only for N-STORM microscopy) 647±1 nm (90 mW) *1: A laser adapter is required for attachment.
  • Page 112 Chapter 5 Laser Unit 5.1 Overview of the Laser Units (1) ND filter slider (C-LU2, C-LU3) Table 5.1-3 Push in OUT: Pull out Brightness In optical path Figure 5.1-2 OUT: Not in optical path 1/32 (2) Light control knob (C-LU3EX only) Mark on the knob Continuous Step...
  • Page 113 Chapter 5 Laser Unit 5.1 Overview of the Laser Units (11) AOTF/PC connector This connector is used to control AOTF of the LU4A four-laser module A. (Do not use in this system.) (12) Interlock cable connectors CONF: Do not use in this system. MIC: This is a connector for the interlock cable to connect the microscope.

  • Page 114: Aom Controller (Optional)

    Chapter 5 Laser Unit 5.1 Overview of the Laser Units 5.1.1 AOM Controller (Optional) Adjusts the brightness control from the software when used in combination with the C-LU3EX three-laser unit Figure 5.1-4 AOM Controller (1) Power switch This is the main power switch of the AOM controller. When this switch is pressed, power is supplied to the primary source only.

  • Page 115: Lu-Lr Four-Laser Ps Rack

    Chapter 5 Laser Unit 5.1 Overview of the Laser Units 5.1.2 LU-LR Four-laser PS Rack The EMISSION key switch (3) on the front panel turns on/off the laser power. The power switch (4) on the rear panel supplies the power to the system. The laser is emitted when the power switch (4) on the rear panel and the EMISSION switch (3) on the front panel are both set on.
  • Page 116 Chapter 5 Laser Unit 5.1 Overview of the Laser Units (3) EMISSION key switch The switch turns on lasers and emits lasers. The key is in vertical position ( O ): The lasers stop emitting and the key can be extracted. The key is in horizontal position ( I ): The lasers start emitting.

  • Page 117: C-Lu2 Two-Laser Unit, And C-Lu3 Three-Laser Unit

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit The setup procedures for two-laser unit and three-laser unit are given below. Preparation Laser installation Laser rough adjustment Final adjustment for transmitting Ar laser light through the optical fiber Final adjustment for transmitting He-Ne laser and 405 nm laser light through the optical fiber Attaching the sheet metal covers 5.2.1...

  • Page 118: Laser Installation

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit 5.2.2 Laser Installation Install the lasers shown in the table described in “5.1. Overview of the Laser Units” onto the specified positions. Ar laser installation Loosen the screw on the mounting plate attached to the laser unit and remove the mounting plate. Fasten it securely to the bottom of the head using the screw provided with the laser head.
  • Page 119 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit NOTE: Leave the lasers turned on for approximately 10 minutes before adjusting. Making adjustments immediately after turning on the lasers may result in shifts from adjusted levels. Two-laser unit Three-laser unit Figure 5.2-2 1-105...

  • Page 120: Laser Rough Adjustment

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit 5.2.3 Laser Rough Adjustment Determine the position of the argon laser by using the optical axis of the farthest He-Ne laser (He-Ne1 laser in Figure 5.2-3 or in Figure 5.2-4) as the reference. Align the lasers so that their laser beams can be transmitted through the optical fiber for the excitation light.
  • Page 121 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Adjusting the lasers Adjustment of the argon laser Mount the dichroic mirror centering tool in place of the dichroic mirror for the argon laser (see Figures 5.2-5 and 5.2-6). Open the shutter for the argon laser.
  • Page 122 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit For the three-laser unit, repeat the procedure above to adjust another He-Ne laser. Adjust only the mounting hardware at the front Shutter to align the laser beam to the centering tool. Dichroic mirror centering tool He-Ne2...
  • Page 123 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Adjusting the angle of the dichroic mirror for the He-Ne laser Mount the dichroic mirror centering tool in place of the dichroic mirror for the argon laser again. Change the direction of the centering tool and adjust the angle of the Hexagonal socket- dichroic mirror for the He-Ne laser located at the far side...
  • Page 124 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Centering the He-Ne laser As shown in Figure 5.2-12, place the target tool at a distance of about 50 cm from the coupler. To perform adjustment of the target tool, move its pinhole so that the beam of the He-Ne laser (He-Ne2 with the three-laser unit, He-Ne1 with the two-laser unit) farthest from the argon laser is aligned with the pinhole of the target tool.
  • Page 125 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Centering the He-Ne1 laser beam in the middle If the He-Ne1 laser is located in the middle of the enclosure, adjust its dichroic mirror so that the He-Ne1 laser beam aligns with the pinhole of the target tool set in the previous step. The accuracy should be within 0.8 mm.
  • Page 126 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit He-Ne2 Polarizer-equipped tool (Push the tool until its edge surface touches the dichroic mirror.) Adjusting screw on the incoming laser side He-Ne1 Adjusting screw on the fiber Coupler Target (such as paper) Rotate polarizer so that the argon Direction of...

  • Page 127: Final Adjustment For The Ar Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit 5.2.4 Final Adjustment for the Ar Laser Light with the Optical Fiber Centering the Ar laser Align the Ar laser beam with the coupler centering tool pinhole. Loosen the clamping screw on the coupler unit. Figure 5.2-14 Set the coupler centering tool pinhole in the laser incident direction, place a paper on the emission...
  • Page 128 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Attaching the fiber coupling Close all laser shutters. Tighten the three screws and attach the fiber coupling unit. Since this will be rotated at a subsequent step, do not tighten excessively at this time.
  • Page 129 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit If even a small amount of laser light is observed, adjust the two fiber side adjustment screws on the coupling to find the point at which the Ar laser light increases in intensity.
  • Page 130 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Adjusting the fiber polarizing orientation Align the knob on the polarizer-equipped tool with the index. Mount the polarizer-equipped tool on the polarizing orientation adjustment tool with the knob at the top.
  • Page 131 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Tighten the three clamping screws on the coupling. Figure 5.2-25 1-117...
  • Page 132 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Adjusting to give maximum Ar laser light transmission through the fiber Attach the emitter end of the optical fiber to the power meter tool and adjust to find the point giving maximum brightness, while measuring the brightness on the power meter.

  • Page 133: Final Adjustment For The He-Ne Laser Light And 405 Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit 5.2.5 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber Transmitting the He-Ne1 laser beam through the optical-fiber cable Without touching the coupler, adjust only the laser body to transmit the laser beam through the optical-fiber cable.
  • Page 134 Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit Transmitting the He-Ne2 laser beam through the optical-fiber cable As with the He-Ne1 laser, adjust the He-Ne2 laser body position to transmit the laser beam at maximum light intensity through the optical-fiber cable. At this time, close the shutters for the argon and He-Ne1 laser so that only the He-Ne2 laser beam enters the optical-fiber cable.

  • Page 135: Attaching The Sheet Metal Covers

    Chapter 5 Laser Unit 5.2 C-LU2 Two-laser Unit, and C-LU3 Three-laser Unit 5.2.6 Attaching the Sheet Metal Covers Install the sheet metal covers. This completes the laser adjustment. 1-121...

  • Page 136: C-Lu3Ex Three-Laser Unit Ex

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX C-LU3EX Three-laser Unit EX The setup procedure for the three-laser unit EX is given below. Preparation Laser Installation Laser Rough Adjustment 1 Laser Rough Adjustment 2 Final Adjustment for the Ar Laser Light with the Optical Fiber Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber Adjusting the AOM Controller (When the AOM Unit Used Only) Attaching the Sheet Metal Cover...

  • Page 137: Preparation

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.1 Preparation Checking accessories Confirm that the following accessories have been provided: • Cable (×2) • Hexagonal head screws (×7) • Harness bands (×6) • Harness band retainers (×4) • Label (×1) •...
  • Page 138 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Attaching the black rings (provided) Remove the bolts and nuts attached at three locations on the laser mounting position opening, holding them to avoid dropping them. Figure 5.3-4 Attach the black (sponge) rings provided, centering them on the opening.

  • Page 139: Laser Installation

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.2 Laser Installation He-Ne laser 405 laser Ar laser (cylindrical type) Overall laser unit diagram DSCN103 Figure 5.3-7 Installing the Ar laser Detach the mounting plate from the laser unit. Attach the mounting plate to the Ar laser. Note that the mounting plate screw positions differ for the 488 multi-line Ar (black) and 488 single-line Ar (white).
  • Page 140 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Installing the He-Ne laser and 405 laser (cylindrical type) Loosen the slot-tightening screw and three sleeve-retaining screws to allow insertion of the laser head. Figure 5.3-10 Insert the He-Ne laser head into the sleeve. To prevent leakage of scattered laser light, press the end of the laser head against the black ring attached to the plate just enough to compress the ring slightly.
  • Page 141 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjust the positions of the units so that the sleeve side face aligns with each mount. Figure 5.3-13 Figure 5.3-14 Check that the laser head shutters are closed (at the CLOSE position). Partially tighten the slot-tightening screw and one of the three sleeve-retaining screws loosened in step 1).

  • Page 142: Laser Rough Adjustment 1

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.3 Laser Rough Adjustment 1 Beam shift unit Light control unit Overall view from above DSCN150 Figure 5.3-15 Checking the initial beam shift unit position Check that the horizontal setscrew tool openings on the sides of the two beam shift units are perpendicular to the light beam, and that the cylindrical glass section is not significantly tilted.
  • Page 143 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Installing the dichroic mirror centering tool (for the EX) Remove the screw, then remove the three dichroic mirror adjustment spring units. Take care to keep the springs from flying off. Spring unit Figure 5.3-18 Remove the two screws (total 6) on each unit, then remove the three dichroic mirror adjustment units.
  • Page 144 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the laser installation position Adjusting the Ar laser Install the dichroic mirror centering tool (for the EX) on the Ar laser dichroic mirror unit, making sure that the tool opening faces in the direction in which the laser is emitted.
  • Page 145 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Repeat the adjustments in steps 3. and 4. until the laser beam passes through the center of the dichroic mirror centering tool opening (for the EX). The centering tolerances should be within approximately 0.2 mm.
  • Page 146 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the He-Ne laser and 405 cylindrical laser Install the dichroic mirror centering tool (for the EX) on the dichroic mirror unit. Open the shutters for the He-Ne laser and 405 cylindrical laser (Set to OPEN position).
  • Page 147 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Attaching the AOM units Turn off power to all units. Remove the tape covering the openings on the AOM units. Detach the two light control units at which the AOM units will be attached, and attach the two AOM units using three screws provided.
  • Page 148 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Wiring the AOM controller Connect the AOM cable provided with the C1-AOM AOM unit to the AOM connector of the AOM controller and that of the laser unit. AOM cable Connect the PC cable supplied with the C1-AOM AOM unit to the connector of the NI card attached to your PC and the PC connector of the AOM controller.
  • Page 149 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the AOM unit Loosen the X-axis, Z-axis, and rotation clamp screws on the AOM unit. Open the laser shutter, and adjust along the X-and Z-axes so that the laser beam passes through the AOM opening.
  • Page 150 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjust the AOM rotation direction. WARNING The AOM controller is connected to the primary power supply. Be careful to avoid touching the primary side. When the AOM controller is turned on, laser light passing through the AOM unit is split into multiple beams.
  • Page 151 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the AOM driver PWR adjustment - for the conventional AOM Connect each part, then set the laser power to 100% on NIS-Elements. Measure the 1st-order light intensity using the power meter to determine the maximum brightness.
  • Page 152 5.3 C-LU3EX Three-laser Unit EX PWR adjustment - for the new AOM New AOM (Checkpoint: the nameplate on the front indicates NIKON only. and the serial number on the nameplate is 59001 or higher) Connect each part, then set the laser power to 100% on NIS-Elements.
  • Page 153 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.4 Laser Rough Adjustment 2 Attaching the dichroic mirror adjustment unit Attach the three dichroic mirror adjustment units, aligning the pin positions and partially tightening the two screws on each unit. The following color mirrors are attached for each laser: •...
  • Page 154 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the Ar dichroic mirror adjustment unit angle (The dichroic mirror adjustment unit adjusts the laser angle.) Place the dichroic mirror centering tool (for the EX) in front of the fiber coupling. Figure 5.3-40 Loosen the two horizontal rotation clamp screws of the Ar dichroic mirror adjustment unit.
  • Page 155 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjust the vertical axis setscrew and align the vertical axis of the laser light with the tool opening position. Figure 5.3-44 Carefully tighten the horizontal clamp screws. Figure 5.3-45 If the beam becomes misaligned from the center after tightening, fine-adjust the vertical setscrew to align the beam with the tool opening.
  • Page 156 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Removing the front sheet metal cover Remove the three screws and remove the front sheet metal cover. Figure 5.3-46 Coarse-adjusting the angle of the other two dichroic mirror adjustment units As a target, prepare a card on which crosshairs are drawn.
  • Page 157 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjust the laser polarization orientations (except for 405 laser) Place the polarizer-equipped tool in front of the fiber coupling with the index facing forward. Open the Ar laser shutter to emit the Ar laser only. Place a white paper behind the fiber coupling and move the polarizer knob to provide minimum light transmission.
  • Page 158 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Fine-adjusting the angle of the other two dichroic mirror adjustment units and adjusting the beam shift unit Adjust the angle of the dichroic mirror adjustment unit and align the He-Ne laser beam position with the target positioned at a distance of 50 cm.
  • Page 159 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjust the beam shift unit to align the positions of the other laser beams with the position of the Ar laser beam scattered by the mirror reflector in front of the fiber coupling.

  • Page 160: Final Adjustment For The Ar Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.5 Final Adjustment for the Ar Laser Light with the Optical Fiber Centering the Ar laser The Ar laser beam is aligned with the coupler centering tool pinhole. Loosen the clamp screw on the coupler unit. Clamp screw (center) Figure 5.3-56 Set the coupler centering tool pinhole in the laser...
  • Page 161 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Attaching the fiber coupling Close all laser shutters. Tighten the three screws and attach the fiber coupling unit. Since this will be rotated at a subsequent step, do not tighten excessively at this time.
  • Page 162 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX If even a small amount of laser light is observed, adjust the two fiber side adjustment screws on the coupling to find the point at which the Ar laser light increases in intensity. (Final fine adjustment will be performed after adjusting the polarizing orientation.) Gradually tighten the fiber connector.
  • Page 163 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting the fiber polarizing orientation Align the knob on the polarizer-equipped tool with the index. Mount the polarizer-equipped tool on the polarizing orientation adjustment tool with the knob at the top. Figure 5.3-63 Clamp the emission end of the optical fiber to the polarizing orientation adjustment tool.
  • Page 164 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Adjusting to give maximum Ar laser light transmission through the fiber Attach the emitter end of the optical fiber to the power meter tool and adjust to find the point giving the maximum brightness, while measuring the brightness on the power meter.
  • Page 165 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Tighten the two clamp screws on the coupler, and then tighten the rotation clamp screw. Figure 5.3-70 Record the Ar laser brightness. (Note the power meter wavelength setting.) Open the other laser shutters and check that laser light is emitted.

  • Page 166: Final Adjustment For The He-Ne Laser Light And 405 Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.6 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber The procedure given below describes how to transmit laser light through the fiber by adjusting the dichroic mirror adjustment unit and beam shift unit alone, without using the coupler.
  • Page 167 Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX Carefully tighten the beam shift unit vertical axis clamp screw. Readjust the rotation axis on the beam shift unit if the brightness decreases here. Then carefully tighten the horizontal axis clamp screw. Horizontal axis clamp screw Figure 5.3-74 Figure 5.3-75...

  • Page 168: Adjusting The Aom Controller (When The Aom Unit Used Only)

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.7 Adjusting the AOM Controller (When the AOM Unit Used Only) Adjusting the AOM controller bias For the adjustment work, the “Ti Tool” of the “Ti Setup Tool Software” is required. Figure 5.3-78 Attaching the AOM controller cover Attach the cover to the AOM controller.

  • Page 169: Attaching The Sheet Metal Cover

    Chapter 5 Laser Unit 5.3 C-LU3EX Three-laser Unit EX 5.3.8 Attaching the Sheet Metal Cover Attach four harness bands to the laser unit mount. Figure 5.3-79 Secure the cables and fibers with harness bands. Harness bands Figure 5.3-80 Attach the sheet metal cover using the six screws. Align the knob with the control range indicator for the light control knob.

  • Page 170: Lu4A Four-Laser Module A

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A LU4A Four-laser Module A The setup procedure for the LU4A four-laser module A is described below. * The procedures on how to setup the previous LU4 model of the four-laser module are described in this section.
  • Page 171 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.1 Preparation Checking accessories Check the accessories (shown in Figure 5.4-2) supplied with the LU4A four-laser module A. • 561 laser adapter • 561 laser adapter cover • 561 Laser setscrew (x4) •...
  • Page 172 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Unscrew each hexagonal bolt on the fixing block at 4 locations four locations, and then remove the four anchoring tools. Hexagonal bolt Figure 5.4-4 Anchoring tool Take a hexagonal bolt of step 3. Insert it into two fixing blocks.
  • Page 173 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Wiring Connect the USB cable supplied with the TI-LU4SU shutter unit between the four-laser module A and the PC. Attaching the provided black rings Remove the bolts and nuts at four locations of the 4 locations laser inlets.
  • Page 174 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Removing the fiber coupling Loosen three knurled screws on the outer side of the optical fiber coupling device and remove the optical fiber coupling device. Handle the coupling carefully to prevent contamination of the space inside. Figure 5.4-12 1-160...

  • Page 175: Laser Installation

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.2 Laser Installation Ar laser installation 488-nm laser adapter Unscrew the four hexagonal bolts on the 488-nm laser adapter attached to the four-laser module A body. And then, detach the 488-nm laser adapter from the four-laser module A body.
  • Page 176 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 405(440)-nm laser installation and 638-nm laser installation Remove six hexagonal bolts (three bolts each) at the L2 position and L1 position of the four-laser module A for the 405-nm (440-nm) laser and 638-nm laser. Mount the 405(440)-nm laser to the L2 position of Fix with hexagonal bolts the four-laser module A.
  • Page 177 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Installing the green HeNe laser Use the polarizer-equipped tool provided with the green HeNe laser and mount the laser so that the polarization orientation of the laser light is vertical. When the polarization orientation of the laser light is vertical at the incident port of the AOTF, the polarization orientation of the laser light is horizontal at the emission port of the AOTF.
  • Page 178 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Loosen two hexagonal bolts at the slots of the green HeNe laser adapters. And then, insert the green Label Slot x 2 HeNe laser into the sleeve of the laser adapters. The laser emission port must contact the black ring to prevent scattered light of laser from leaking.
  • Page 179 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 561-nm solid-state laser installation Normally, the green HeNe laser adapters are attached to the four-laser module A at the product is shipped from the factory. To use the 561-nm solid-state laser, loosen four hexagonal bolts of the green HeNe laser adapters and remove the green HeNe laser adapters along with the hexagonal bolts.
  • Page 180 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 405-nm (Coherent) solid-state laser installation Attach a black ring to the L2 laser inlet of the LU4A body. Figure 5.4-27 Install the 405C N-STORM laser adapter (MXA22115) to L2 of the LU4A. Figure 5.4-28 Install the laser to the supplied laser adapter part (small) with the supplied four screws so that the laser...
  • Page 181 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 561-nm (Coherent) solid-state laser installation This product is shipped from the factory with the G-HeNe laser adapter installed to the laser module as standard equipment. To use the 561-nm solid-state laser, loosen the four hexagonal bolts of the G-HeNe laser adapter and remove the G-HeNe laser adapter with the hexagonal bolts.
  • Page 182 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 647-nm (Crystalaser) solid-state laser installation Attach a black ring to the L1 laser inlet of the LU4A body. Figure 5.4-33 Install the 647-nm laser adapter to L1 of the LU4A. (Tilt the laser itself 45 degrees to match the laser output polarization.) Figure 5.4-34 Install the laser to the laser adapter with the screws...
  • Page 183 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Oscillation method: (1) Confirm that the knob on the front of the control box has been fully turned counterclockwise and that the key switch is turned off. (2) Turn on the switch on the rear of the control box. (3) Turn on the key switch on the front of the control box.
  • Page 184 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Laser warm-up and AOTF warm-up Remove transparent tape at the inlet and outlet of Transparent tape the AOTF unit. Figure 5.4-38 Warm up the lasers and the AOTF (1) Turn off the [REMOTE] switch on the back of the laser unit.

  • Page 185: Laser Rough Adjustment 1

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.3 Laser Rough Adjustment 1 Checking the initial beam shift unit position The tool hole and the horizontal clamp screw on the Tool hole side of the beam shift part must be aligned vertically. The cylindrical glass must not tilt significantly.
  • Page 186 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Table 5.4-2 Laser wavelengths available with the AOTF driver remote controller On the screen Wavelength Laser 457 nm 477 nm Ar laser 488 nm 514 nm 543 nm Green HeNe laser 561 nm 561-nm solid-state laser 405/440 nm...
  • Page 187 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A If the collimation pin comes with the laser coupling mirror part, take the collimation pin from the laser coupling mirror part and restore the pin into the hole again. Attach the centering tool to the collimation pin. Rotate the centering tool so that the laser light enters the hole of the centering tool at a right angle.
  • Page 188 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A The laser light direction can be adjusted horizontally by moving the laser body with hands. To complete the adjustment, follow the procedures described below under each module name. Figure 5.4-47 <for LU4A> (Do not use in this system.) Hole L (1) Attach the centering tool to the laser module at Hole S...
  • Page 189 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Tighten temporarily Fully loosen the clamp screw at the right of the Ar laser mirror adjustment part viewed from the laser coupling mirror part to the laser body. And loosen the clamp screw to the halfway point at the left of the Ar laser mirror adjustment part.
  • Page 190 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Vertical clamp screw 10. Loosen the clamp screw for the horizontal direction (nearer to the axis A) of the L3 beam shift part. Vertical setscrew (farther from the axis A) 11. The laser light direction can be adjusted horizontally by rotating the whole L3 beam shift part by hands.
  • Page 191 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 0th-order light Light-shielding plate 16. Check that the 0th-order light from the AOTF unit is shielded by the shield plate. And then, close the shutter of the optical path for the Ar laser light. If the 0th-order light is not shielded, the position of the shield plate must be adjusted.
  • Page 192 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Adjusting other lasers To adjust the green HeNe laser or the 561-nm solid-state laser, select the laser wavelength with the numeric key on the AOTF remote controller. * To adjust the green HeNe laser, select L5 (543 nm).
  • Page 193 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Reattach the dichroic mirror adjustment part and Position B Centering tool attach the centering tool to position B. Adjust the Hole L dichroic mirror adjustment part in both horizontal and vertical directions so that the laser light enters the hole L.
  • Page 194 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Position C Laser coupling mirror part 10. Attach the centering tool to position C and adjust the Centering tool Hole L laser coupling mirror part in both horizontal and vertical directions so that the laser light enters the hole L.
  • Page 195 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Vertical clamp screw Loosen the clamp screw for the horizontal direction (nearer to the axis A) of the L4 beam shift part. Vertical setscrew (farther from the axis A) Aim the laser light at the hole of the centering tool attached in step 4 by rotating the L4 beam shift part.
  • Page 196 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 16. Fully loosen the clamp screw that fixes the setscrew for the vertical direction adjustment of the L4 dichroic mirror adjustment part. Figure 5.4-74 17. Aim the laser light at the hole of the centering tool attached in step 3 in "3.
  • Page 197 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.4 Laser Rough Adjustment 2 Adjusting the AOTF driver Refer to the data sheet provided with the laser unit. Adjust and register the frequency and power settings of the AOTF driver for the four wavelengths of the Ar laser, the wavelength of each of the green HeNe laser and the 561-nm solid-state laser (and 405-nm (440-nm) laser and 638-nm laser for LU4A).
  • Page 198 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A In case of the LU4A, remove the fiber coupling and attach the power meter to the far end of the laser Attach the power meter with a tape. module body with a tape. It enables you to perform the AOTF remote controller adjustment while measuring the laser power.
  • Page 199 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Press the [STR] key on the AOTF driver remote controller after adjustments. Settings are stored into the AOTF driver. Press [ESC] key to again display the wavelength number for the laser beam to be emitted. Another press on the key turns the display to “external mode.”...
  • Page 200 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Adjusting the laser coupling mirror part Adjust the optical path of the Ar laser (488 nm). The optical path of the Ar laser is used as a reference. Optical paths of other lasers are aligned with it. Restore the laser coupling mirror part to its original position.
  • Page 201 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Vertical adjustment hole Slightly loosen the two clamp screws for the vertical direction adjustment on the laser coupling mirror part. Insert a screwdriver into the adjustment hole for the vertical direction adjustment on the laser coupling mirror part.
  • Page 202 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Aligning each laser beam to the Ar laser beam In this section, the green HeNe laser and the 561-nm solid-state laser are described as examples. Put a target such as a piece of paper at 50 cm or farther away from the outlet of the laser of the optical fiber coupling device.

  • Page 203: Final Adjustment For The Ar Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.5 Final Adjustment for the Ar Laser Light with the Optical Fiber Centering the Ar laser Knurled screws Loosen three knurled screws at the optical fiber coupling device. Insert the coupler centering tool into the optical fiber coupling device so that its pinhole side faces the laser incident side.
  • Page 204 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Adjustment screw on the H fiber side Rotate the adjustment screws "H" and "V" at the optical fiber side to maximize the laser intensity. Adjustment screw on the V fiber side Figure 5.4-94 Repeat Step 5 and Step 6 to maximize the laser intensity.
  • Page 205 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Transmitting Ar laser light through the fiber WARNING Never look directly at the fiber tip. Always monitor laser emissions by aiming the tip down onto a sheet of paper. Carefully align the end of the optical fiber (the end without the tag) with the key groove in the coupling and insert as far as it will go.
  • Page 206 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Adjusting to give maximum Ar laser light transmission through the fiber Measure the intensity of the Ar laser light with the power meter. Adjust the beam shift part and the screws, "H" and "V," on the optical fiber side of the optical fiber coupling device to get the maximum intensity.
  • Page 207 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Fixing the beam shift part and the optical fiber coupling device Tighten the clamp screws for the horizontal direction Vertical clamp screw and the vertical direction to fix the L3 beam shift part. (nearer to the axis A) Rotate by hand Horizontal clamp screw...

  • Page 208: Final Adjustment For Other Laser Light With The Optical Fiber

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.6 Final Adjustment for Other Laser Light with the Optical Fiber Other laser lights than the Ar laser have been adjusted by performing up to step 3 "Aligning each laser beam to the Ar laser beam"...
  • Page 209 Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A Vertical setscrew Rotate the setscrew for the adjustment in the vertical direction on the L4 dichroic mirror adjustment part and adjust the angle to reach the maximum intensity of the laser light. Figure 5.4-107 Repeat Step 3 to Step 6 to maximize the laser intensity.

  • Page 210: Removing The Aotf Driver Remote Controller

    Chapter 5 Laser Unit 5.4 LU4A Four-laser Module A 5.4.7 Removing the AOTF Driver Remote Controller When all the adjustments have been made, press the [ESC] key of the AOTF driver remote controller once or twice to display “external mode” on the screen.

  • Page 211: Attaching The Sheet Metal Cover

    Figure 5.4-111 Install the sheet metal covers. Figure 5.4-112 Attach each side panel and fix it with six screws each. (The Nikon marking is indicated on the back part.) Nikon marking Figure 5.4-113 Affix provided stickers to indicate laser wavelengths.

  • Page 212: Setting Up The Lu-Lr Four-Laser Ps Rack

    Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Setting Up the LU-LR Four-laser PS Rack CAUTION The LU-LR four-laser PS rack is heavy. The laser rack weighs approximately 30 kg with laser supplies unmounted. Do not attempt to lift or carry the four-laser PS rack by yourself.
  • Page 213 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Components (accessories included) 1. LU-LR four-laser PS rack Figure 5.5-1 2. Clamp for laser power supplies Figure 5.5-3 Figure 5.5-4 Figure 5.5-2 [1] For Ar laser [2] For HeNe laser [3] For laser diodes and DPSS laser Option...
  • Page 214 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack 4. Keys for the EMISSION switch Figure 5.5-7 5. Ferrite core Figure 5.5-9 Figure 5.5-8 [1] For Ar laser cable [2] For Ar laser remote controller cable 1-200...

  • Page 215: Mounting Laser Power Supply

    Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack 5.5.2 Mounting Laser Power Supply Mounting positions, clamps, and cables vary with the type of laser power supply. Select the clamp positions, clamp types, and cables suitable for each laser supply. Power supplies mounted Table 5.5-2 Mounting Position Wavelength...
  • Page 216 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Position of laser power supplies 1. Positions of laser power supplies (1) Ar, HeNe, Laser diode x 2 [1] Ar laser Front Rear Control box of LU-LR [3] HeNe laser [2] Laser diodes four-laser PS rack Figure 5.5-10...
  • Page 217 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack (3) DPSS, HeNe, Laser diode x 2 [4] DPSS laser (controller) [5] DPSS laser (power supply) Rear Front Control box of LU-LR four-laser PS rack [2] Laser diodes [3] HeNe laser Figure 5.5-12 (4) DPSS x 2, Laser diode x 2...
  • Page 218 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack 2. Positions of cables [4] HeNe laser power cable [3] Ar laser power cable (HeNe 543 nm) (IMA Ar Laser) [1] Laser diode power cable [2] Laser diode power cable (56ICS-638nm) (56ICS-405nm, 56ICS-440nm) Rear...
  • Page 219 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Mounting procedure 1) Remove the side panel, the top panel, and the port panel on the rear side of the LU-LR four-laser PS rack. ↓ 2) Mount required laser power supplies into the four-laser PS rack. For details, refer to Chapter 3.
  • Page 220 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack INTERLOCK connector Port for cables AC inlet (15A type) Ferrite core for Ar laser AC inlet (5A type) Figure 5.5-15 Ar laser remote controller Ferrite core for the Ar laser remote controller Figure 5.5-16 Bundle disused cables and secure it...

  • Page 221: Details On Mounting Laser Supply

    Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack 5.5.3 Details on Mounting Laser Supply Mounting laser diodes (56ICS series) Turn on the key switch and the power switches of the laser diode heads located outside the four-laser PS rack. Secure the laser diode power supplies in the positions [2] with the provided clamps [3].
  • Page 222 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Mounting Ar laser (IMA series) Turn on the key switch and the power switch of the Ar laser power supply. Additionally, move the POWER ADJUST control of the Ar laser power supply fully clockwise. Secure the Ar laser power supply in the positions [1] with the provided clamps [1].
  • Page 223 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Connect the cable (to be connected to the Ar laser remote controller) to the LU-LR four-laser PS rack control box located in the rack through the cable port on the rear side. LU-LR four-laser PS rack Remote controller cable (To the Ar laser head)
  • Page 224 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Mounting DPSS laser (85BCD/85YCA series) Turn on the key switch of the DPSS laser controller. Secure the DPSS laser controller in the position [4] with screws. (Fix the DPSS laser controller directly to the bottom plate without clamps.) Secure the DPSS laser power supply in the position [5] with the provided clamps [3].
  • Page 225 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack RC cable for the DPSS laser LU-LR four-laser PS rack (85BCD/YCA RS232C) Figure 5.5-24 1-211...
  • Page 226 Chapter 5 Laser Unit 5.5 Setting Up the LU-LR Four-laser PS Rack Mounting HeNe laser (25LGP series) Turn on the key switch of the HeNe laser power supply. Secure the HeNe laser power supply in the position [3] with the provided clamp [2]. Connect the power cable [4] (HeNe-543nm) to the HeNe laser power supply.

  • Page 227: Connection Between The Laser Unit And The Microscope

    Connection between the Laser Unit and the Microscope Setting and Connecting the TI-LUSU Shutter Unit (6) Safety cover signal connector (8) Laser unit connector (4) Interlock cable connectors (9) Serial IF connector (3) AC adapter connector (7) Safety cover close INPUT RS-232C LASER UNIT...

  • Page 228: Control System Selection

    Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit 6.1.1 Control System Selection The settings of the TI-LUSU shutter unit must be set up in accordance with the system configuration, the case for the TI-TIRF or the TI-TIRF-E or the case for the TI-PAU.

  • Page 229: Setting And Connecting The Ti-Lu4Su Shutter Unit Lu4

    Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit 6.1.3 Setting and Connecting the TI-LU4SU Shutter Unit LU4 Option switch Laser unit connector Indicator lamp TI-LU4SU LASER EMISSION POWER Power lamp Laser shutter switch Figure 6.1-2 Settings...
  • Page 230 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit Cable of the Nylon clamp laser unit TI-LU4SU LASER EMISSION Connecting to the LU4A POWER TI-LU4SU Spacer Fixing bolts Nylon clamp Cable of the laser unit Figure 6.1-3 1-216...

  • Page 231: Dip Switch Settings

    Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit 6.1.4 DIP Switch Settings (1) DIP switch S6 functions and settings in the factory default conditions Table 6.1-1 Function Description Factory default Safety cover switch enabled: TIRF Safety cover switch disabled: PAU Safety cover switch...
  • Page 232 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit (3) DIP Switch Locations Detach the four screws on the bottom of the shutter unit to open the bottom plate with the circuit board. The DIP switches are mounted on the lower left part of the circuit board viewed from the operation switch side.

  • Page 233: Communications Commands

    Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit 6.1.5 Communications Commands (1) Standard commands The following are factory default commands and can be used in the standard condition. The shutter unit controls laser shutters so that only one shutter opens at a time in the standard condition. c command: Operation, control, and data setting command Table 6.1-3 o: Normal...
  • Page 234 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit r command: Data request command Table 6.1-4 a: Normal r command response Description n: Error response The command reads the shutter condition. (1): [0] means all shutters are closed.
  • Page 235 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LUSU Shutter Unit (2) Shutter independent control commands The following commands are enabled when the TI-LUSU shutter unit is set to the shutter independent control mode.

  • Page 236: Attaching The Optical Fiber To The Light Source

    Chapter 6 Connection between the Laser Unit and the Microscope 6.2 Attaching the Optical Fiber to the Light Source Attaching the Optical Fiber to the Light Source Using straight fiber (part used in PAU illuminator unit) Attach the straight fiber adapter when using a straight fiber, which has a straight tip, rather than C1 single-mode fiber (note that you cannot connect using this method with the photo activation illuminator unit).
  • Page 237 Chapter 6 Connection between the Laser Unit and the Microscope 6.2 Attaching the Optical Fiber to the Light Source Using C1 single mode fiber Draw the optical fiber through the round ring metal cover and attach the optical fiber to the FC connector. When inserting the cable, be sure to align it with the FC connector's notch, and then fully screw in the knurled section.

  • Page 238: Replacement Of Optical Path Switch Mirror

    Chapter 6 Connection between the Laser Unit and the Microscope 6.3 Replacement of Optical Path Switch Mirror Replacement of Optical Path Switch Mirror To replace the optical path switch mirrors, which switch between the laser light and epi-fl illumination light, perform the following.
  • Page 239 Chapter 6 Connection between the Laser Unit and the Microscope 6.3 Replacement of Optical Path Switch Mirror Remove the two holder plate locking screws. And then, Holder plate locking screws remove the holder plate and flat spring to replace the mirrors.

  • Page 240: Installing The N-Storm Kit

    Chapter 6 Connection between the Laser Unit and the Microscope 6.4 Installing the N-STORM Kit Installing the N-STORM Kit Installation method Install the laser specified for the N-STORM kit to the LU4A through the dedicated laser adapter. Install the Ti-E fixing plates and the DSC support columns of the N-STORM kit. Install the 3D-STORM port, STORM slider, lambda plate slider, and N-STORM filter cube.
  • Page 241 Chapter 6 Connection between the Laser Unit and the Microscope 6.4 Installing the N-STORM Kit Operating the N-STORM optical system When the optical system for N-STORM microscopy is installed, the ND filter of the laser adapter, the STORM slider, the lambda plate slider, and the cylindrical lens of the 3D-STORM port must be inserted or removed according to the microscopy method as shown in the following table.

  • Page 242: Confirmation And Check Sheet

    Confirmation and Check Sheet Checking Procedure The system is a FDA Class 3B and EN Class 3B laser product. The laser devices are FDA Class 3B and EN Class 3b components. The final confirmation for the laser safety standards should be done after setting up. Put a laser safety label on the prescribed place after confirming (1) Laser intensity power The recommended lasers can be put on the laser unit (see Table 5.1-1).

  • Page 243: Check Sheet

    Chapter 7 Confirmation and Check Sheet 7.2 Check Sheet Check Sheet The person setting up the system should check the details listed in the sheet below and enter the results. The completed sheet should be sent to the supervisor. Table 7.2-1 Serial number TI-TIRF TIRF Illuminator Unit/TI-TIRF-E Motorized TIRF Illuminator Unit/TI-PAU...
  • Page 244 Chapter 7 Confirmation and Check Sheet 7.2 Check Sheet Safety checks Table 7.2-4 Unit Check items Check Is there a gap between the unit and laser head? Is the protective cover securely attached? Laser and laser unit Does any laser light leak from gaps in the protective cover? Is the sheet metal cover attached? Is the optical fiber securely fixed? Are all the retaining screws on the accessories (Field diaphragm unit,...

  • Page 245: Specifications And Performance

    Specifications and Performance Overall Configuration 8.1.1 TIRF Illuminator Unit / Motorized TIRF Illuminator Unit― Configuration for Normal Microscopy (Except N-STORM) Table 8.1-1 Unit Device Model name Remarks Ti-E Ti-E/B Use the TI-DH Dia Pillar Illuminator 100W. Ti-U Ti-U/B Apo TIRF 60×H/1.49 Apo TIRF 100×H/1.49 Plan Apo TIRF 60×H/1.45 Objective for TIRF...

  • Page 246: Motorized Tirf Illuminator Unit - Configuration For N-Storm

    Chapter 8 Specifications and Performance 8.1 Overall Configuration Unit Device Model name Remarks Two-laser unit C-LU2 Three-laser unit C-LU3 Laser unit See Table 8.1-3 for mountable lasers. Three-laser unit EX C-LU3EX Four-laser module A LU4A Filter slider cover Filter cube port cover retaining plate Ti laser safety kit Safety label...
  • Page 247 Chapter 8 Specifications and Performance 8.1 Overall Configuration Unit Device Model name Remarks Commercially available (specified item) TIRF image Monitor Commercially available observation devices TV camera Commercially available An adapter is required to connect to the (video camera) (specified item) microscope port.

  • Page 248: Photo Activation Illuminator Unit

    Chapter 8 Specifications and Performance 8.1 Overall Configuration 8.1.3 Photo Activation Illuminator Unit Table 8.1-3 Unit Device Model name Remarks Ti-E Ti-E/B Use the TI-DH Dia Pillar Illuminator Ti-U 100W. Ti-U/B Use objectives appropriate for Objective specimens. TI-LUSU The unit can be connected to a PC. Shutter unit TI-LU4SU AD-1260B...

  • Page 249: Mountable Lasers

    Chapter 8 Specifications and Performance 8.1 Overall Configuration 8.1.4 Mountable lasers For the beam divergence and the maximum power, see the manual for each laser device. Table 8.1-4 Normal Microscopy (Except N-STORM) Mountable unit and Type Device Laser specifications position Manufacturer: Melles Griot C-LU2 (L1)* Head: 56 RCS series (56RCS/S2780)
  • Page 250 Chapter 8 Specifications and Performance 8.1 Overall Configuration Mountable unit and Type Device Laser specifications position Manufacturer: Melles Griot C-LU2 (L2) HeNe laser Head: 05LGP193 C-LU3 (L3) 543.5 nm (1 mW) (green) Power supply: 05LPL951-065: for 100V C-LU3EX (L3) 05LPL915-065: for 115/230V LU4A (L4) Manufacturer: Melles Griot C-LU2 (L2)*...
  • Page 251 Chapter 8 Specifications and Performance 8.1 Overall Configuration Table 8.1-5 N-STORM Mountable unit and Type Device Laser specifications position Manufacturer: Coherent Diode laser 405±5 nm (100 mW) LU4A (L2)* Head: CUBE series (CUBE405) (violet) Power supply: CUBE series Manufacturer: Melles Griot Air-cooled Head: IMA101 series 457 to 514 nm (65 mW)

  • Page 252: Ti-Lusu Shutter Unit

    Chapter 8 Specifications and Performance 8.1 Overall Configuration 8.1.5 TI-LUSU Shutter Unit Table 8.1-6 Model name TI-LUSU Input ratings 12 VDC, 1A 8.1.6 AC Adapter for the TI-LUSU Shutter Unit Table 8.1-7 Model name AD-1260B EA1050E-120 Manufacturer Medi-Power Electronics Inc. (Taiwan) EDAC POWER Electronics Co., Ltd.

  • Page 253: Environmental Conditions

    Chapter 8 Specifications and Performance 8.2 Environmental Conditions Environmental Conditions 8.2.1 Normal Microscopy (Except N-STORM) Operating conditions Table 8.2-1 Temperature 5 to 35°C Relative humidity 60% RH maximum with no condensation Altitude 2,000 m maximum Pollution degree Degree 2 Installation category Category II Class I Electric shock protection class...

  • Page 254: Safety Standards Compliance

    Chapter 8 Specifications and Performance 8.3 Safety Standards Compliance Safety Standards Compliance 8.3.1 Normal Microscopy (Except N-STORM) Table 8.3-1 • This product complies with IEC60825-1 Class 3B requirements. Laser safety • This product complies with FDA Class 3B requirements. • This product meets EU Low Voltage Directive requirements. •...

  • Page 255: N-Storm

    Chapter 8 Specifications and Performance 8.3 Safety Standards Compliance 8.3.2 N-STORM Table 8.3-2 • This product complies with IEC60825-1 Class 3B requirements. Laser safety • This product complies with FDA Class 3B requirements. • This product meets EU Low Voltage Directive requirements. CE marking •...
  • Page 257 Part 2 For Setting Up TI-TIRF-PAU and LU4-B5 Series Setup Manual <For Authorized Nikon Personnel>...
  • Page 259 Contents System Configurations and Part Names.................... 2-3 1.1 Overview ............................2-3 1.2 Optical Paths of Lasers ........................2-5 1.2.1 Laser Unit........................... 2-5 1.2.2 Microscope Part (TIRF-PAU Illuminator and Ti-E System)..........2-6 1.3 Safety Labels..........................2-7 1.3.1 Ti-U Microscope Body......................2-8 1.3.2 Ti-E Microscope Body ......................
  • Page 260 Contents 8.2 Environmental Conditions ......................2-70 8.3 Safety Standards Compliance...................... 2-71...

  • Page 261: System Configurations And Part Names

    System Configurations and Part Names Overview The following illustrates the standard configuration to use the TIRF-PAU illuminator in combination with the Ti-E. You can also refer to the following when using Ti-U instead of Ti-E as the configuration is substantially the same except the microscope.
  • Page 262 Chapter 1 System Configurations and Part Names 1.1 Overview TIRF-PAU illuminator and Ti-E system overview (top view) (15) (11) (10) (14) (16) (12) (13) Figure 1.1-2 * For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.” (1) Power supply devices for the laser devices (9) Power supply device for the diascopic illumination...

  • Page 263: Optical Paths Of Lasers

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers Optical Paths of Lasers 1.2.1 Laser Unit LU4A four-laser module A (with LU4-B5 Beamsplitter 50/50 installed) Figure 1.2-1 (1) Laser mounting position L1 (5) Motorized shutters (See the table below.) (6) Laser coupling mirror (2) Laser mounting position L2 (7) Laser outlet (single-mode optical fiber)

  • Page 264: Microscope Part (Tirf-Pau Illuminator And Ti-E System)

    Chapter 1 System Configurations and Part Names 1.2 Optical Paths of Lasers 1.2.2 Microscope Part (TIRF-PAU Illuminator and Ti-E System) TIRF-PAU illuminator and Ti-E system (top view) Laser light Laser light Figure 1.2-2 TIRF-PAU illuminator and Ti-E system (front left view) Optical fiber Laser safety cover Laser light...

  • Page 265: Safety Labels

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels Safety Labels (1) IEC warning label - laser hazard (2) IEC explanatory label - Class 3B laser (3) Model name and serial number label symbol product TIRF-PAU LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT (4) Safety standard label (5) IEC caution label - Class 3B area...

  • Page 266: Ti-U Microscope Body

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels 1.3.1 Ti-U Microscope Body The following safety labels are affixed to the product. Before using the product, check that individual labels are affixed at their correct positions. (1) IEC warning label - laser hazard (2) IEC explanatory label - Class 3B laser (6) IEC caution label - Class 3B area symbol...

  • Page 267: Ti-E Microscope Body

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels 1.3.2 Ti-E Microscope Body The following safety labels are affixed to the product. Before using the product, check that individual labels are affixed at their correct positions. (1) IEC warning label - laser hazard (2) IEC explanatory label - Class 3B laser (6) IEC caution label - Class 3B area symbol...

  • Page 268: Lu4A Four-Laser Module A (With Lu4-B5 Beamsplitter 50/50 Installed)

    15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause LU4A harmful interference,and NIKON CORPORATION, JAPAN (2) this device must accept any interference received, including interference that may 100-240V~ 50/60Hz 60001 cause undesired operation.

  • Page 269: Lu-Lr Four-Laser Ps Rack

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels 1.3.4 LU-LR Four-laser PS Rack The following safety label is affixed to the product. Before using the product, check that the label is affixed at the correct position. (10) Caution label (Front view) (10) (Rear view)

  • Page 270: Ti-Lu4Su Shutter Unit Lu4

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels 1.3.5 TI-LU4SU Shutter Unit LU4 The following safety labels are affixed to the product. Before using the product, check that individual labels are affixed at their correct positions. (4) Safety standard label (9) TIRF-PAU special label TIRF This device complies with Part 15 of the FCC Rules.

  • Page 271: Tirf-Pau Illuminator

    Chapter 1 System Configurations and Part Names 1.3 Safety Labels 1.3.6 TIRF-PAU Illuminator The following safety labels are affixed to the product. Before using the product, check that individual labels are affixed at their correct positions. (3) Model name and serial number label (5) IEC caution label - Class 3B area (6) IEC caution label - Class 3B area CAUTION –...

  • Page 272: Name Of Each Part

    Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Name of Each Part TIRF-PAU illuminator and Ti-E system TI-TIRF-PAU Filter slider cover TIRF-PAU illuminator (part of laser safety kit) TI-FLC-E or TI-FLC-E/HQ motorized epi-fl filter turret, with To laser unit Protection plate (part of laser safety kit) Laser safety cover...
  • Page 273 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TIRF-PAU illuminator (20) (10) (16) (14) (17) (13) (12) (15) (11) (18) (19) Figure 1.4-3 Parts used in the TIRF microscopy operation Parts used in the epi-fl microscopy operation (1) Optical fiber (12) Aperture diaphragm centering screws...
  • Page 274 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-RCP remote control pad (When Ti-E or Ti-E/B used only) User can perform the following operations using the remote control pad: • TIRF/EPI button: The optical path is changed between laser irradiation (TIRF or PAU illumination) and episcopic illumination.
  • Page 275 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Laser safety kit The laser safety kit is supplied with all laser Filter slider cover products mounted onto a Ti microscope. Dia pillar illuminator Labels affixed to each laser product include a name label and other types of necessary labels, both of which are not shown here.
  • Page 276 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Protection plate (for nosepiece) The protection plate (for nosepiece) is a component designed for shielding the gap between the nosepiece and the epi-fl filter turret to avoid accidental jamming of fingers inserted into the gap or the laser radiation from the gap.
  • Page 277 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Optical path selector switch (Ti-E, Ti-E/B) The laser shutter does not open when the binocular route of the optical path (“EYE”) is selected. Ti-E Observation port 100% FL Block switch L100 Left port 100% (filter cube selector)
  • Page 278 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Four-laser module A (LU4A) (1) Laser mounting position L1 (2) Laser mounting position L2 (3) Laser mounting position L3 (4) Laser mounting position L4 (5) Single-mode fiber (6) Power switch (7) AC inlet (8) Remote switch (9) CONTROLLER connector...
  • Page 279 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Four-laser PS rack (LU-LR) (1) EMISSION laser indicator (2) POWER laser indicator (3) EMISSION key switch (4) Power switch (5) INTERLOCK connector (6) Cooling fan (7) Power breaker for 15 A line (8) Power breaker for 5 A line (9) Caution label Front view...
  • Page 280 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part TI-BPU back port unit Adding a camera port by attaching the TI-BPU back port unit to the microscope base allows for a concurrent image capture with two cameras. Attach a C mount camera via a separate C mount adapter to the back port unit and perform focus adjustment and centering.
  • Page 281 When attaching to the lower tier, embed the TI-TIRF stage up lens into the illuminator unit to correct the condensing position and provide the optimum illumination performance. Contact your own Nikon representative for details on the stage up lens. The following figure shows the Ti-U with the stage up kit mounted, along with the TIRF-PAU illuminator unit...
  • Page 282 Chapter 1 System Configurations and Part Names 1.4 Name of Each Part Positioning pin Stage support block Nosepiece support block Epi-fl filter turret Shielding plate Epi-fl filter turret support block (left) support block (right) Positioning pin Dia-illuminator pillar support block Support pillar extension TI-BSUK70 70 mm stage up kit Figure 1.4-19...

  • Page 283: Preparations

    Preparations Check Items and Tools For items and tools required, refer to 2.1, “Check Items and Tools” in Chapter 2 of Part 1. 2-25...

  • Page 284: Overview Of Setup Procedure

    Chapter 2 Preparations 2.2 Overview of Setup Procedure Overview of Setup Procedure Setting Up the PC and Operation Software (Chapter 3) • Attaching the light source (TI-TIRF-PAU) Setting Up the Microscope • Attaching the dichroic mirror (TI-PAU only) (Chapter 4) •...

  • Page 285: Setting Up The Pc And Operation Software

    Setting Up the PC and Operation Software Using TIRF and PAU microscopy, images are observed on a monitor, via a camera attached to the microscope camera port. TIRF images and laser port images from PAU microscopy cannot be observed through the eyepieces. Make sure to set up the PC and operation software for the camera to be used. 2-27...

  • Page 286: Setting Up The Microscope

    Setting Up the Microscope Overall Configuration Table 4.1-1 Unit Device Model name Remarks Ti-E Ti-E/B Use the TI-DH Dia Pillar Illuminator 100W. Ti-U Ti-U/B TIRF 60xH/1.49 TIRF 100xH/1.49 Plan Apo TIRF 60xH/1.45 Objective for Select the appropriate objective for TIRF microscopy observations.

  • Page 287: Setup Procedure

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Setup Procedure 4.2.1 Assembling Each Part and Attaching Them to the Microscope Perform the steps below to assemble each part and attach them to the microscope. ⇒ Page 2-30 TIRF-PAU illuminator installation ⇒...
  • Page 288 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure TIRF-PAU illuminator installation Remove the clamping screws 1 and 2 from the part used in PAU illumination operation. Press the part used in PAU illumination operation into the microscope to secure it. Attach the two support legs (for two areas) on the part used in PAU illumination operation to adjust the height.
  • Page 289 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the dichroic mirrors (photo activation illuminator unit only). It is necessary to attach a dichroic mirror (optical path switch mirror), which switches between laser light and episcopic illumination, inside the photo activation illuminator unit. Since the attachment does not ship with a mirror attached, you must attach one following the procedure below.
  • Page 290 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the field diaphragm unit. Remove the rubber cap on the left side of the Locking screw microscope. Insert the field diaphragm unit. Tighten the locking screw using an Allen wrench, and secure the field diaphragm unit.
  • Page 291 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Assemble the remaining microscope parts. Refer to the instruction manual included with the microscope main body before assembling the remaining microscope parts. Attach the laser safety cover (TIRF illuminator unit only). If you are using the TIRF illuminator unit, the motorized shutter will not open unless the laser safety cover is attached on the stage.
  • Page 292 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attach the filter cubes. You can attach up to six filter cubes in the epi-fl filter turret. Remove the filter cube port cover, check the turret Indication of address address, and insert the filter cubes along the guide on the optical path (position for ID sticker) groove.
  • Page 293 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Affix the labels. After confirming the safety of the laser (p.2-64 to 2-66), affix the specified warning labels to the locations shown below. Left side of microscope main unit Affix “(7) IEC caution label - Class 3B area” on the left side of the microscope main unit, under the field diaphragm.
  • Page 294 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure On the laser safety cover (TIRF illuminator unit) Make sure that the following three warning labels at the rear left, when viewed from the Binocular eyepiece tube: “(1) IEC warning label - laser hazard symbol”, “(2) IEC explanatory label - Class 3B laser product”, and “(8) IEC aperture label 1”...

  • Page 295: Attaching The Laser Safety Kit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Laser unit Remove the laser safety label if it is attached to the laser unit. Remove the laser safety labels. About 12 mm About 20 mm About 12 mm About 30 mm Figure 4.2-13 4.2.2 Attaching the Laser Safety Kit...
  • Page 296 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure If the Perfect Focus System is not used Remove the two M3 hexagon socket head M5 Hexagon TI-N6 (6 nosepiece), bolts secured on top of the epi-fl filter turret socket head TI-ND6 (DIC 6 nosepiece), or (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ), using TI-ND6-E (motorized DIC 6 nosepiece)

  • Page 297: Attaching The Stage Up Kit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.3 Attaching the Stage Up Kit By attaching the TI-BSUK70 70 mm stage up kit and doubly mounting the epi-fl filter turrets, you can attach the TIRF-PAU illuminator unit and TI-BPU back port unit simultaneously. Attach the stage up kit immediately after attaching the eyepiece base unit and the epi-fl filter turret on the lower tier.
  • Page 298 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Hexagon socket head bolt Hexagon socket head bolt (M5, two) (M5, four) Shielding plate Hexagon socket head bolt (M5, two) Stage support block Dia-illuminator pillar support Hexagon socket head button bolt block (M3, four) Epi-fl filter turret support block...
  • Page 299 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attaching the support pillar extension To attach the TIRF-PAU illuminator unit to the upper tier, attach the support pillar extension supplied with the stage up kit to the support pillar supplied with the illuminator unit. Support pillar extension (supplied with the stage up kit) Support pillar...

  • Page 300: Attaching The Back Port Unit

    Chapter 4 Setting Up the Microscope 4.2 Setup Procedure 4.2.4 Attaching the Back Port Unit Insert the back port unit into the guide on the rear of the microscope body and use the hexagonal screwdriver (4-mm) to secure it from behind by tightening screws. •...
  • Page 301 Chapter 4 Setting Up the Microscope 4.2 Setup Procedure Attaching the back port extension kit When combined with the TIRF-PAU illuminator unit, you need to attach the TI-BP-EX back port extension kit and shift the camera mount position backward. Split the TI-BPU back port unit. Remove the M4-10 hexagon socket head bolts (four) from the microscope connection side to split it.

  • Page 302: Laser Unit

    Laser Unit WARNING • The unit must be set up as instructed by the local LSO (Laser Safety Officer). • This laser unit uses Class 3B lasers. Accidental exposure of eyes or skin to laser light may result in injury or other problems. Do not turn on the lasers until they are installed in the laser unit •...

  • Page 303: Overview Of The Laser Units

    Chapter 5 Laser Unit 5.1 Overview of the Laser Units Overview of the Laser Units 5.1.1 LU4A Four-laser Module A (with LU4-B5 Beamsplitter 50/50 installed) This laser unit is mounted with four kinds of lasers (with one laser product per color) selected from the lasers listed in Table 8.1-2 Recommended lasers.
  • Page 304 Chapter 5 Laser Unit 5.1 Overview of the Laser Units (6) Interlock cable connectors CONF: Do not use in this system. MIC: This connector is for the interlock cable to connect a microscope. TIRF: This connector is for a cable to connect the laser safety cover. (7) LUSU connector This connector is for cables to connect the shutter unit LU4.
  • Page 305 Chapter 5 Laser Unit 5.1 Overview of the Laser Units Table 5.1-1 Mountable position Product name LU4A four-laser module Manufacturer: Melles Griot 56 RCS series (56 RCS/S 2780) 400 to 410 nm (36 mW) Manufacturer: Melles Griot 56 RCS series (56 RCS/S 2781) 440 to 445 nm (20 mW) Manufacturer: Coherent CUBE series (CUBE405)
  • Page 306 Chapter 5 Laser Unit 5.1 Overview of the Laser Units Table 5.1-2 Adapter list MXA22070 (C-LAR488 laser adapter) Special adapter Special adapter 2-48...

  • Page 307: Lu-Lr Four-Laser Ps Rack

    Chapter 5 Laser Unit 5.1 Overview of the Laser Units 5.1.2 LU-LR Four-laser PS Rack An EMISSION key switch (3) on the front panel turns on/off the laser power. The power switch (4) on the rear panel supplies the power to the system. The laser is emitted when the power switch (4) on the rear panel and the EMISSION switch (3) on the front panel are both set on.
  • Page 308 Chapter 5 Laser Unit 5.1 Overview of the Laser Units (3) EMISSION key switch The switch turns on lasers and emits lasers. The key is in vertical position ( O ): The lasers stop emitting and the key can be extracted. The key is in horizontal position ( I ): The lasers start emitting.

  • Page 309: Installation Of The Lu4-B5 Beamsplitter 50/50 (Tirf-Pau System)

    Chapter 5 Laser Unit 5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) The procedure for installing the LU4-B5 beamsplitter 50/50 into the LU4A four-laser module A is described below. For the setup procedure for the LU4A four-laser module A, refer to 5.4, “LU4A Four-laser Module A” in Chapter 5 of Part 1.
  • Page 310 Chapter 5 Laser Unit 5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) Attaching the beamsplitter 50/50 (1) Remove the reference shutter located behind the AOTF for the LU4A four-laser module A Attach the shutter. Reference shutter Figure 5.2-3 (2) Attach the shutter. Remove the reference shutter detached from the optical path on the reference optical fiber side.
  • Page 311 Chapter 5 Laser Unit 5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) Figure 5.2-7 Adjusting the reference optical fiber Manually open the shutter with a tool. Adjust the optical axis so that the 488-nm laser beam, the reference for other lasers, passes through the centering tool placed in front of the fiber coupling device.
  • Page 312 Chapter 5 Laser Unit 5.2 Installation of the LU4-B5 Beamsplitter 50/50 (TIRF-PAU system) Attaching the fiber cover (1) Attach the fiber cover. Attach the light shield to the fiber. Light shield Figure 5.2-10 (2) Attach the fiber cover plate. Fiber cover plate Figure 5.2-11 (3) Attach the fiber cover block.
  • Page 313 15 of the FCC Rules. Operation is subject to the following two conditions: LU4A (1) this device may not cause harmful interference,and NIKON CORPORATION, JAPAN (2) this device must accept any interference received, including interference that may 100-240V~ 50/60Hz cause undesired operation.

  • Page 314: Connection Between The Laser Unit And The Microscope

    Connection between the Laser Unit and the Microscope Setting and Connecting the TI-LU4SU Shutter Unit LU4 Option switch Laser unit connector Indicator lamp TI-LU4SU LASER EMISSION POWER Power lamp Laser shutter switch Figure 6.1-1 Settings Settings are not required. Connecting the TI-LU4SU unit Connect the cable of the laser unit to the TI-LU4SU shutter unit LU4.
  • Page 315 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LU4SU Shutter Unit LU4 Cable of the Nylon clamp laser unit TI-LU4SU LASER EMISSION Connecting to the LU4A POWER TI-LU4SU Spacer Fixing bolts Nylon clamp Cable of the laser unit Figure 6.1-2 2-57...
  • Page 316 Chapter 6 Connection between the Laser Unit and the Microscope 6.1 Setting and Connecting the TI-LU4SU Shutter Unit LU4 Affixing the switch label If the LU4-B5 beamsplitter 50/50 is attached to the four-laser module A, affix the TIRF-PAU special label supplied with the TI-LU4SU shutter unit LU4.

  • Page 317: Attaching The Optical Fiber To The Light Source

    Chapter 6 Connection between the Laser Unit and the Microscope 6.2 Attaching the Optical Fiber to the Light Source Attaching the Optical Fiber to the Light Source Using straight fiber (part used in PAU illuminator) Attach the straight fiber adapter when using a straight fiber, which has a straight tip, rather than C1 single-mode fiber.
  • Page 318 Chapter 6 Connection between the Laser Unit and the Microscope 6.2 Attaching the Optical Fiber to the Light Source Attaching the Fiber Loosen the two M4 setscrews, and remove the round ring metal cover. Draw the optical fiber through the round ring metal cover and attach the optical fiber to the FC connector.

  • Page 319: Replacement Of Optical Path Switch Mirror

    Chapter 6 Connection between the Laser Unit and the Microscope 6.3 Replacement of Optical Path Switch Mirror Replacement of Optical Path Switch Mirror To replace the optical path switch mirrors, which switch between the laser light and epi-fl illumination light, perform the following.
  • Page 320 Chapter 6 Connection between the Laser Unit and the Microscope 6.3 Replacement of Optical Path Switch Mirror Remove the two holder plate locking screws. And then, Holder plate locking screws remove the holder plate and flat spring to replace the mirrors.

  • Page 321: Cable Connections (Four Laser Module A)

    Chapter 6 Connection between the Laser Unit and the Microscope 6.4 Cable Connections (Four Laser Module A) Cable Connections (Four Laser Module A) Connect cables between units, as shown in Figure 6.4-1 and Table 6.4-1. 4LA7 L1 to L4 4LA1 4LA4 4LA6 4LA3...

  • Page 322: Confirmation And Check Sheet

    Confirmation and Check Sheet Checking Procedure The system is a FDA Class 3B and EN Class 3B laser product. The laser devices are FDA Class 3B and EN Class 3b components. The final confirmation for the laser safety standards should be done after setting up. Put a laser safety label on the prescribed place after confirming (1) Laser intensity power The recommended lasers can be put on the laser unit.

  • Page 323: Check Sheet

    Chapter 7 Confirmation and Check Sheet 7.2 Check Sheet Check Sheet The person setting up the system should check the details listed in the sheet below and enter the results. The completed sheet should be sent to the supervisor. Table 7.2-1 Serial number TI-TIRF-PAU Illuminator Shutter unit (model name:...
  • Page 324 Chapter 7 Confirmation and Check Sheet 7.2 Check Sheet Safety checks Table 7.2-4 Unit Check items Check Is there a gap between the unit and laser head? Is the protective cover securely attached? Laser and laser unit Does any laser light leak from gaps in the protective cover? Is the sheet metal cover attached? Is the optical fiber securely fixed? Are all the retaining screws on the accessories (Field diaphragm unit,...

  • Page 325: Specifications And Performance

    Specifications and Performance Overall Configuration Table 8.1-1 Unit Device Model name Remarks Ti-E Ti-E/B Use the TI-DH Dia Pillar Illuminator 100W. Ti-U Ti-U/B TIRF 60xH/1.49 TIRF 100xH/1.49 Plan Apo TIRF 60xH/1.45 Objective for Select the appropriate objective for TIRF microscopy observations.
  • Page 326 Chapter 8 Specifications and Performance 8.1 Overall Configuration Mountable lasers For the beam divergence and the maximum power, see the manual for each laser device. Table 8.1-2 Mountable unit and Type Device Laser specifications position Manufacturer: Melles Griot Head: 56 RCS series (56 RCS/S 2780) 400 to 410 nm (36 mW) Power supply: 56 IMA series (56 IMA021) Manufacturer: Melles Griot...
  • Page 327 Chapter 8 Specifications and Performance 8.1 Overall Configuration Mountable unit and Type Device Laser specifications position Manufacturer: Melles Griot Head: 56 ICS series (56 ICS/S 2669) 638 nm (10 mW) Power supply: 56 IMA series (56IMA022) Diode laser (red) Manufacturer: Coherent Head: CUBE series (CUBE640) 640+2/-5 nm (40 mW)
  • Page 328 Chapter 8 Specifications and Performance 8.2 Environmental Conditions Environmental Conditions Operating conditions Table 8.2-1 Temperature 5 to 35°C Relative humidity 60% RH maximum with no condensation Altitude 2,000 m maximum Pollution degree Degree 2 Installation category Category II Electric shock protection class Class I Indoor use only Storage conditions...
  • Page 329 Chapter 8 Specifications and Performance 8.3 Safety Standards Compliance Safety Standards Compliance Table 8.3-1 • This product complies with IEC60825-1 Class 3B requirements. Laser safety • This product complies with FDA Class 3B requirements. • This product meets EU Low Voltage Directive requirements. CE marking •...

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