Nikon CSU-W1 User Manual
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Nikon CSU-W1 User Manual

Nikon CSU-W1 User Manual

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SPINNING DISK NIKON CSU-W1
USER MANUAL
SWITCH ON THE SPINNING DISK ..................................................................................................... 2
SWITCH ON THE TEMPERATURE CONTROLLER ............................................................................... 3
MICROSCOPE FRAME CONTROLS .................................................................................................... 4
EYEPIECE OBSERVATION ................................................................................................................. 5
SPINNING DISK ACQUISITION .......................................................................................................... 6
ND ACQUISITION ............................................................................................................................. 8
ACQUISITION SAVING .................................................................................................................... 17
SWITCH OFF THE SPINNING DISK .................................................................................................. 18
SWITCH OFF THE TEMPERATURE CONTROLLER ........................................................................... 19
CSU-W1_EN_V1 05/2024
Page 1 / 19

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  • Page 1: Table Of Contents

    SPINNING DISK NIKON CSU-W1 USER MANUAL SWITCH ON THE SPINNING DISK ..................... 2 SWITCH ON THE TEMPERATURE CONTROLLER ................3 MICROSCOPE FRAME CONTROLS ....................4 EYEPIECE OBSERVATION ......................... 5 SPINNING DISK ACQUISITION ......................6 ND ACQUISITION ..........................8 ACQUISITION SAVING ........................17 SWITCH OFF THE SPINNING DISK ....................

  • Page 2: Switch On The Spinning Disk

    Switch on the frame of the microscope. This switch is located on the right of the microscope Turn the key of spinning disk CSU-W1 Turn the key of the laser Once you turn on the computer, start the "NIS Elements" software, choose the user session and click on «...

  • Page 3: Switch On The Temperature Controller

    SWITCH ON THE TEMPERATURE CONTROLLER Open the CO2 cylinder Switch on the temperature/CO2 controller display (push and maintain the button) Adjust various parameters (temperature ; CO2 percentage ; fan speed etc) CSU-W1_EN_V1 05/2024 Page 3 / 19...

  • Page 4: Microscope Frame Controls

    MICROSCOPE FRAME CONTROLS Select the objective ESC allows you to lower the objective. Use it before changing an objective with an immersion. Then, you can push this button again to upper the objective Choose how you want to observe your sample. Here, green light is on eyepieces 4- Optovar lens up to 1.5x There is no coarse or fine focus.

  • Page 5: Eyepiece Observation

    EYEPIECE OBSERVATION Select the illumination mode Select the objective Adjust the power of each LED Adjust the power of the white lamp CSU-W1_EN_V1 05/2024 Page 5 / 19...

  • Page 6: Spinning Disk Acquisition

    SPINNING DISK ACQUISITION I. Acquisition settings Select the illumination mode (Triggered allows you to observe multiple dye at the same time) Camera settings (binning ; exposure time ; ROI) Select the number of laser line you want and adjust the power of each Choose the observation mode Click on live to make the...
  • Page 7 II. Contrast Adjust the gate of your contrast Auto-contrast Check if there is any satured pixels Choose the color for your image CSU-W1_EN_V1 05/2024 Page 7 / 19...

  • Page 8: Nd Acquisition

    ND ACQUISITION I. Time acquisition Select « Time » Add as many phase as you want and set the parameters (interval ; duration) CSU-W1_EN_V1 05/2024 Page 8 / 19...
  • Page 9 II. Multiples positions acquisition Select « XY » Add as many position as you want Click on « include z » to add the third coordinate CSU-W1_EN_V1 05/2024 Page 9 / 19...
  • Page 10 III. Use Autofocus Hardware (PFS : Perfect Focus System) Select « Use PFS ». By selecting « Use PFS », you will be able to change the offset value. Unclick « Include Z » Click on the arrow to set the PFS value Offset CSU-W1_EN_V1 05/2024 Page 10 / 19...
  • Page 11 IV. Lambda Select « λ » Add as many illumination as you want. Click or unclick on an illumination to acquire it or not CSU-W1_EN_V1 05/2024 Page 11 / 19...
  • Page 12 V. Z-stack a) Top and Bottom Select « Z » Select top and bottom Check on live the bottom and the top value Choose the recommanded step size If you use the PiezoZ for the z-stack, the range limit is 500 µm. Above this value, you as to change for the Ti2 CSU-W1_EN_V1 05/2024 Page 12 / 19...
  • Page 13 b) Symmetric Select « symetric » The software will do the half of the range you entered above and below the actual z. Click on « Home » to set the center and on « Relative » if you are using multiplepositions. c) Asymmetric Select «...
  • Page 14 VI. Large Image Choose a large image Choose the shape of the tile according to the form of your sample Let « Optimal Path » Click on « Run » When the acquistion is finished, you can make a right click on the image and choose «...
  • Page 15 You can also do it in the « ND Acquisition tab »: Click on « Large Image » Choose the size of the field. Here, you can’t choose the shape as the first method Let « Overlap » to 15% and the Stitching on « Optimal path » CSU-W1_EN_V1 05/2024 Page 15 / 19...
  • Page 16 VII. Run experiment Select the order of experiement, this window can be used until two acquisition modes are selected. This step allows you to choose an order in the acquisition to be faster Check the order according to the model chosen Click on «...

  • Page 17: Acquisition Saving

    ACQUISITION SAVING Select the image Click on « Save As… » CSU-W1_EN_V1 05/2024 Page 17 / 19...

  • Page 18: Switch Off The Spinning Disk

    SWITCH OFF THE SPINNING DISK Close NIS and turn off the computer Turn the key of the laser Turn the key of spinning disk CSU-W1 Switch off the frame of the microscope. This switch is located on the right of the microscope The main switch on the wall must remain in the «...

  • Page 19: Switch Off The Temperature Controller

    SWITCH OFF THE TEMPERATURE CONTROLLER Switch off the temperature/CO2 controller display (push and maintain the button). Switch it off before turning off the microscope (fourth step of « switch off the spinning disk ») Close the CO2 cylinder CSU-W1_EN_V1 05/2024 Page 19 / 19...

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