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TC 9639 Thermal Cycler User Manual Document Version 1.4 99 Washington Street Melrose, MA 02176 Phone 781-665-1400 Toll Free 1-800-517-8431 Visit us at www.TestEquipmentDepot.com...
About This Manual Please read the manual carefully before using the Thermal Cycler! Purpose of This This manual provides instructions on how to set up and use the Benchmark Thermal Manual Cycler. Benchmark reserves the right to modify this manual at any time without prior notice.
Powers on (1) and powers off (0) the instrument. Power Inlet Connects the instrument to power socket via supplied power cable. Ethernet Port Connects up to 255 Thermal Cyclers to a computer. RS232 Port Connects the instrument to a computer. Benchmark Thermal Cycler User Manual...
Enter a number A number button. Enter a letter A letter button. Touch to change between lowercase letters and uppercase letters. Join several words Underscore character Delete the last character in an input field Erase all characters Benchmark Thermal Cycler User Manual...
• User Manual (this manual) • Warranty registration card (to be completed and returned) IMPORTANT! If the Thermal Cycler is damaged or some items are missing or damaged, contact Benchmark Scientific. 4. Move to the desired installation site. 1.3 Power On and Power Off 1.
Then continue to turn the lid knob clockwise until you hear ‘click …’ sound. The lid is now pressing on the reaction vessels. Benchmark Thermal Cycler User Manual...
Go to list of folders, which contain all saved programs. Program Library Creates new program quickly. Program Wizard Sets instrument parameters and shows other tools. System Setting Configures shortcut functions programs. Empty Shortcut Shows a selected program shortcut. Program Shortcut Benchmark Thermal Cycler User Manual...
When plugging a flash drive into the USB port, a USB icon appears. Touch this icon to open the USB folder. The USB folder contains programs that are stored in the USB flash drive. Programs can be copied to and from the USB flash drive. Benchmark Thermal Cycler User Manual...
2. Touch Yes in the displayed pop-up window to delete the folder. Note: The Preset folder, Public folder and USB folder cannot be deleted. IMPORTANT! When you delete a folder, all programs saved in the folder will be deleted at the same time. Benchmark Thermal Cycler User Manual...
2. In the Rename Program screen, Clear the old program name, enter a new name, and then touch Ok to accept changes. Note: The program name is not case sensitive. Programs in the Preset folder cannot be renamed. Benchmark Thermal Cycler User Manual...
Touch Clear to clear the program list. Note: The Rename and Copy buttons are not available in the Last Run Programs screen. Benchmark Thermal Cycler User Manual...
Items in the Edit Program screen: a: Step number. b: Temperature for a constant temperature step. c: Step holding time (hour: min: sec). d: The start step number and amounts of additional repeats for cycles. Benchmark Thermal Cycler User Manual...
(ie a larger sample volume requires a longer time to reach temperature). Block mode does not consider sample volume and simply heats the block to the set temperature. Benchmark Thermal Cycler User Manual...
(ie hot start) or when adding or removing tubes during a step. • Touching Forever will cuase the program to hold indefinately after reaching this step until it is stopped manually. Steps cannot be added after a forever step. Benchmark Thermal Cycler User Manual...
5. Touch Ok to accept changes and close the screen. 1.4 Set Cycle 1. In the Edit Program screen, select a step that you want to set as the last step of a cycle. 2. Touch Goto and the Cycle Step screen opens. Benchmark Thermal Cycler User Manual...
3. From the Folder drop-down list box, select a folder to save the program to. • To create a new folder to save the program, touch New Folder, and enter a Benchmark Thermal Cycler User Manual...
The Program Wizard function provides a quick method to create a standard three-step or two-step PCR program and perform running. 1. In the Home screen, touch Program Wizard. The Program Wizard screen opens. There are two screens, touch Next and Last to shift between them. Benchmark Thermal Cycler User Manual...
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PCR reaction with 30 cycles, then you should enter a number of 30. 4. Touch Save to save program or touch Run and then select whether save the program before running according the displayed message in the pop-up window. Benchmark Thermal Cycler User Manual...
After you touch Run, the Set Run Options screen opens. 2. In the Set Run Options screen, edit Temperature Control and Lid Temperature Control. 3. Touch StartRun to begin the run. The Run screen opens. Benchmark Thermal Cycler User Manual...
‘Temp’. After run, the temporary program will not be recorded in the list of the Last Run Programs screen. c: Options: Pause: preset pause; X °C/Cyc: Increment or decrement temperature; X S/Cyc: Increment or decrement time; X °C/S: Ramp rate. d: The recent run message. Benchmark Thermal Cycler User Manual...
The actual remaining time may slightly differ from that shown. View Bar Chart 1. In the Run screen, touch Gradient to view the temperature of each column of of Sample Block sample block in real-time. Temperature Benchmark Thermal Cycler User Manual...
Stop Run In the Run screen, touch Stop and then touch Yes in the displayed pop-up window. Run Program In the Run screen, if the running program stopped, touch Run Again to run it Again again. Benchmark Thermal Cycler User Manual...
To view product information about the Thermal Cycler: 1. In the Home screen, touch System Setting. 2. In the System Setting screen, touch About and the About screen appears. 3. When you finish, touch Ok to return to the System Setting screen. Benchmark Thermal Cycler User Manual...
The Tm Calculator is used to calculate the Tm (melting temperature) and Ta Calculator (annealing temperature) for a primer pair. 1. In the Home screen, touch System Setting. 2. In the System Setting screen, touch Tm Calculator to open the screen. Benchmark Thermal Cycler User Manual...
When login is enabled, each user can configure different shortcuts. To configure shortcuts: 1. In the Home screen, touch: • Any button labeled with ‘Shortcut’ • System Setting, then Configure Shortcut. The Configure Shortcut screen opens. Benchmark Thermal Cycler User Manual...
When login is enabled, only admin can see the Admin Options button To use Admin Options functions: In the Home screen, touch System Setting, then Admin Options. The Admin Options screen opens. Benchmark Thermal Cycler User Manual...
5. Touch Ok, and restart the instrument by turning off the power switch. After enabling the Access Control, you must login as a user on the Login screen (see below) before you can use the instrument. Benchmark Thermal Cycler User Manual...
2. In the System Setting screen, touch Admin Options. 3. In the Admin Options screen, touch User Message. 4. The User Message screen lists all accounts currently available. Touch the table column header to sort all users. Benchmark Thermal Cycler User Manual...
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Edit Password screen. Enter new password and touch Ok. c. To delete a user account, select a user and then touch Delete. Touch Yes in the displayed pop-up window to delete the user. Benchmark Thermal Cycler User Manual...
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99 Washington Street Melrose, MA 02176 Phone 781-665-1400 Toll Free 1-800-517-8431 Visit us at www.TestEquipmentDepot.com...