Section 7. Internet Programming Instructions; Entering New System Software Or Run Parameters - Biocomp GRADIENT MASTER 108 Operating Manual

6. Using weight as your guide, remove the sample volume from the top of the tube, sucking a
mixture of air and water so that you stay at the air-water interface.
7. Carefully layer the sample on top of the gradient and seal the tube.
III. Entering run parameters:
If you have ordered an 8-place tube holder, your Gradient Master will have a rotor called VERT in the
LIST menu of rotors. Its list of runs is initially empty, but will have had one run programmed into it at
the factory. There are a few runs developed specifically for the quick-seal tubes at the end of the run
parameter list in the manual for the same size open top tube. For example, the VTi50 vertical rotor
runs are found at the end of the SW28-32 run parameters, since these two rotors use the same
diameter tube. As it turns out, most of the regular open top swinging bucket rotor runs are nearly the
same as the same run developed for the quick seal tubes of the same diameter. Thus, we have
programmed into your Gradient Master the open top tube runs.
1. Option 1: Use the open top runs as they are. Go to the equivalent open top rotor in LIST, find the
gradient you need and run it.
2. Option 2: Using an open top run, but saving it in the VERT rotor list. Find the run in the open top
LIST (i.e. SW28 Long cap 20-50% Nycodenz) and start and quickly stop the run (this enters the run
parameters into the LAST memory buffer). Go back to LIST, press the VERT rotor key and then press
NEW. The previous (LAST) run will appear in the register. Go through the data entry without
changing anything and Start the run. Exit and Save the run and it will appear in the VERT rotor list.
See Section G. above for the programming instructions.
3. Option 3: Manually entering a Vertical run from the Gradient List in the manual. At the bottom
of the open top list of the same diameter tube, you will find the quick-seal tube runs that have been
developed in that tube. Follow the instructions in Section G. to enter these parameters in the VERT list
of runs as in Option 2 here.

SECTION 7. INTERNET PROGRAMMING INSTRUCTIONS

ENTERING NEW SYSTEM SOFTWARE OR RUN PARAMETERS.

1. Software updates are typically sent by email.
2. Locate the SD card that we shipped with your new Gradient Master. It has the original files on it to
reload old software if ever the need arises. For updates and new rotor keys, you will need to load the
new files on it while removing older versions with the same name. Use the SD card reader (Lexar)
provided with your instrument to load files from the USB port on your PC or Mac
Here is a list of the files as they should be labelled on the SD card:
Cgmcodea.s19
CGRADINA.s19 This is the current run parameter listing for all rotors (i.e. Gradlist8)
SDGMprga.s19
SDGRproga.s19 This tells the software where to load the run parameters (page 1)
The keys that unlock the gradient holders you have purchased are listed as follows: (see the rotor
table on pages 7 & 8 for a complete listing of equivalent rotors).
Crr281a.s19
Crsw28a.s19
Crsw40a.s19
Crsw41a.s19
Crsw50a.s19
Crsw60a.s19
Crtl55a.s19
This is the main software version (i.e.5.22)
This tells the software where to put the main program on the flash chip (page 4)
SW28.1
SW28, SW32
SW40
SW41
SW50, SW55, SW65
SW60
TLS-55
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