Biocomp GRADIENT MASTER 108 Operating Manual page 16

2. Press the rotor you wish to develop a gradient for (only gradients you have the software key for will
appear):
3. In order for the gradient to be stored in the correct location in the LIST, you will need to describe its
basic properties. Words like Short, Sucr and wv are selected from lists that appear when you press the
EDIT key when that character is blinking. Numbers are entered using the numerical keypad. Here
are the words that will appear when you press SCROL after pressing the EDIT key for that character:
4. When the proper value is showing, press ENTR. Pressing the NEXT key will scroll through the values
on the EDIT screen.
5. Pressing EDIT on % light and heavy will bring up the screen:
6. If you enter a number incorrectly, keep pressing the number keys until the proper values appear.
Press ENTR when the desired value is in place.
7. When all the descriptors are adjusted, press the DONE key:
8. Entering the Run Parameters for each step.
The Model 108 returns to a feature that was present in model 106 that saves a great deal of time and
effort when entering runs with many steps. If you examine at the run parameters at the back of this
manual, you will find runs with 10, 14 and even 21 steps. A few years ago, we discovered that
excellent gradients with high % bottom solutions (>45% sucrose, for example) could be made using a
technique we call START-STOP.
Here is an example from the SW41 catalog: Short cap 5-50% wv sucrose
Short Sucr 02-28% wv
EDIT NEXT DONE
CAP SOLUTE
Short Sucr
Long Glyc
CsCl
Dext
Fico
KBr
Koac
Metr
NaAc
NaCl
Nyco
Opti
Perc
Reno
Usr0
Short Sucr 02-28% wv
ENTR SCROL
Short Sucr 02-28% wv
ENTR Use Num Keypad EXIT
Short Sucr 02-28% wv
EDIT NEXT DONE
LO-HI% UNITS
0-99 vw
vv
ww
14
EXIT
EXIT
EXIT