Fluigent FLOW-RATE PLATFORM User Manual page 21

Make a table that contains the time for each measurement, the flow-rate of the pump and the data measured
by the FLOW UNIT. A minimum of 3 measurements is recommended for each flow-rate.
The principle of the experiment is to inject the FC-40 through the desired FLOW UNIT model connected to the
FLOWBOARD. Then simultaneously you record the flow-rate given by the software and you measure the weight
of fluid you have collected over a chosen period of time. Knowing the density of the fluid, you are able to
define the actual flow-rate.
Note that if a peristaltic or a syringe pump is used, one has to wait until the target flow-rate is reached (settling
times can be long) and to calculate an average flow-rate due to the pulsations.
The list of materials needed to reproduce the experiment is given below:
- One (1) FLOWBOARD
- One (1) FLOW UNIT model
- One (1) MFC"™-EZ or with the appropriate pressure range (1 bar for FC-
software (or later versions)
- One (1) precision weighing scale
The table below displays the information recorded during the experiment: the pressure imposed by the
MFC"™-EZ, Qs the flow-rate recorded by the FLOW UNIT through the Flow-Rate Platform software, Qw the
flow-rate measured with the precision weighing scale, and Qw/Qs the calculated scale factor for a single point
calibration.
Pressure (mbar)
596.3
Consequently, when working around 317 µl/min (target flow-rate), you have to add the scale factor of 3.5 so
that the measurement of the sensor corresponds to the actual flow-rate for FC-40.
SA à Directoire et Conseil de Surveillance au capital de 141 284.95 € - Siret : 487 636 409 00038 – N°TVA UE/EU VAT Number : FR 53 487 636 409
Q (µl/min)
S
91.6
Siège social : Biopark – 1 mail du Professeur Georges Mathé – 94800 Villejuif – France
Tel : +331 77 01 82 68 – Fax : +331 77 01 82 70
Q (µl/min)
w
317.8
FLUIGENT
www.fluigent.com
a d Maesflo™ .
Q /Q
w S
3.5
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