Fluigent FLOW-RATE PLATFORM User Manual page 19

7.1.4 Recommendations for fluids
Working with Multiple Liquids
Switching between multiple liquids can leave transient deposits in the form of liquid layers inside the glass
capillary. This is especially common for insoluble liquids, but can happen even with miscible liquid
combinations. For example, when IPA is followed by water in a sensor without drying in between, large offsets
can be observed for hours after switching to water.
If possible, dedicate a separate sensor for each different liquid to be measured. If not possible, use caution
when switching media and clean properly.
Working with Water
When working with water it is recommended not to let the sensor dry out. All salts and minerals in the water
will deposit on the glass and are difficult to remove. Although salt solutions are particularly prone to problems,
even clean water can still contain enough dissolved minerals to form a deposition layer. Flush with DI water on
a regular basis to prevent build-up. If you still encounter problems, occasionally flush the sensor with slightly
acidic cleaning agents.
When working with water containing organic materials (sugars, etc.) microorganisms often grow on the walls
of the glass capillary and form an organic film that can be difficult to remove. Flush on a regular basis with
solvents such as ethanol, methanol or IPA, or with cleaning detergents to remove organic films.
Working with Silicone Oils
When working with silicone oil it is recommended not to let the sensor dry out. Silicone oils can be cleaned out
using special cleaners. Check with your silicone oil supplier for cleaning agents compatible with glass surfaces.
Working with Paints or Glues
When working with paints or glues it is critical not to let the sensor dry out. Often, depositions of paints and
glues cannot be removed anymore after they have dried. Flush the sensor with cleaning agents recommended
by your paint or glue manufacturer that are compatible with glass. Ensure that you have found a good cleaning
procedure before performing the first tests, and always clean shortly after emptying the sensor.
Working with Alcohols or Solvents
Unlike most other fluids, alcohols and solvents are not critical and a short flush of isopropanol (IPA) is sufficient
to clean the capillary walls.
Other Liquids or Applications
If uncertain about your application and how to clean the flow sensor, please contact FLUIGENT for additional
support at support@fluigent.com
Identified cleaning solutions
Sample liquid
Biofilm/cells
1% micro-beads of
polystyrene in DI Water
Mineral oil (Sigma cat no.
Blood
SA à Directoire et Conseil de Surveillance au capital de 141 284.95 € - Siret : 487 636 409 00038 – N°TVA UE/EU VAT Number : FR 53 487 636 409
.
Cleaning solution


Biofilm remover
Sodium dichloroisocyanurate (1
ppm HClO; ref : 218928)
Toluene 99.8% (ref : 244511)
RBS 25 (ref : 83460)
5904) 

BD FACS Clean
25 (ref : 83460)
RBS
Siège social : Biopark – 1 mail du Professeur Georges Mathé – 94800 Villejuif – France
Tel : +331 77 01 82 68 – Fax : +331 77 01 82 70
FLUIGENT
www.fluigent.com
Supplier


Umweltanalytik
Sigma Aldrich
Sigma Aldrich
Sigma Aldrich


BD
Sigma Aldrich
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