Adjustment Of Eucentric Plane; Adjustment Of Beam Incidence - LEO 912 OMEGA Brief Manual

Transmission electron microscope
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Microscope Operation
1.5.6
1.5.7
6
Shift spot to the index point of the small screen with the encoders X,Y in
the field ILLUMINATION.
Repeat the beam alignment till the spot positon is identical at 5 nm and
100 nm spot size.
Push button STORE GUN ALIGNM. and answer PROCEED ? with Y(es).
Return to main menu with button RETURN and push button ILL. MODE to
select TEM illumination.
– The BEAM ALIGNMENT function is automatically deactivated, when leaving
the GUN menu.

Adjustment of eucentric plane

Move specimen rod all the way in.
Activate AIS mode in the main menu.
Insert an objective aperture and center it (see chapter "Center objective
aperture").
Select magnification 25.000x and move specimen detail to the center of the
small screen.
Calibrate objective lens with button CALIBRATE in the field FOCUS.
Switch on focus aid with button AID.
– Illuminated ON indicates FOCUS AID is active; specimen detail is moving.
Focus specimen mechanically using Z-CONTROL buttons, until specimen
detail does not move any more.
– Specimen is now in the eucentric plane of the goniometer.
Switch off FOCUS AID with button AID.

Adjustment of beam incidence

SPECIAL NOTE:
This adjustment step is very important for a single field condenser/objective lens.
Asymmetric diffraction fringes are created by a tilted illumination.
Deactivate AIS mode in the main menu.
Select magnification 63.000x.
Basic alignment
LEO 912 OMEGA

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