Saturation Pulse Analysis; Five Fluorescence Levels - Walz MINI-PAM-II Manual

5

Saturation Pulse Analysis

5.1

Five Fluorescence Levels

Usually, five different levels of modulated fluorescence are ac-
quired by PAM fluorometers. Two of these levels (F
must be measured with the dark-acclimated sample. The three
other levels (F
exposed sample or in a dark period following this light treatment
(see Fig. 22, page 31). Some parameters of saturation pulse
analysis require fluorescence measurement of the same sample
Fig. 22: Measurements for Saturation Pulse Analysis. AL, Actinic Light; D,
dark; SP, Saturation Pulse; FR, Far red illumination.
Saturation Pulse Analysis
', F, and F ') are measured with the actinic light-
0 M
Chapter 5
and F )
0 M
31