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MicroBio Operating Manual [P0001W006]
Throughout the trials, Bioquell demonstrated that bio-decontamination with H2O2 vapour does not
appear to be detrimental in any way, effect operational aspects or aesthetics of sensitive electronic
equipment. For further information on Bioquell H2O2 vapour Material Compatibility please refer to
their document BDS-3-MATCOMP-V3.2 available from their website
www.bioquell.com
or contact
them on +44 (0)1264 835835.
Calibration
It is recommended that the MicroBio should be regularly calibrated in accordance with specific
industry best practice. Typically this will be on the anniversary of the instrument entering service.
The only calibration adjustment on the instrument is the flow rate, by default set to 100 litres per
minute.
A validation/calibration kit (Cantium Scientific Limited part number P0059) for the MicroBio MB1 and
MB2 sampler is also available from your local distributor, or the sampler may be returned to your
local distributor or Cantium Scientific Limited.
MicroBio Air Sampling
Although the use of simple settle plates can be used for collection of bacterial and fungal spores, it
can never give a quantitative determination. This passive technique will also fail to enumerate very
small particles such as bacteria, which will remain suspended.
The MicroBio MB1 or MB2
bioaerosol samplers allow a quantitative measurement to be made.
Sampling
The selection of the sampling volume is important for reliable sampling.
If the contact plate is
overloaded with colonies it is difficult to make an accurate count. With experience, the user will
anticipate the probable bioaerosol concentration in an area, but it may be necessary to make a
preliminary survey at a number of sampling volumes to identify the optimum sampling volume. Each
sample should be repeated several times and a statistical mean value and confidence value for the
value determined.
June 2012
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