Photon Systems Instruments AquaPen-C AP 110-C Manual And User Manual
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Photon Systems Instruments AquaPen-C AP 110-C Manual And User Manual

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AquaPen-C AP 110-C
AquaPen-P AP 110-P
Manual and User Guide
Please read this manual before operating this product
PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic
FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz
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  • Page 1 AquaPen-C AP 110-C AquaPen-P AP 110-P Manual and User Guide Please read this manual before operating this product PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz...
  • Page 2 © PSI (Photon Systems Instruments), spol. s r. o. © PSI (Photon Systems Instruments), spol. s r. o. (hereinafter PSI), 2018 This document and its parts can be copied or provided to a third party only with the express permission of PSI.

  • Page 3: Table Of Contents

    © PSI (Photon Systems Instruments), spol. s r. o. TABLE OF CONTENTS Information Before Using AquaPen Device ........................5 Technical Specification ..............................6 General Information ............................... 8 Device Description ..............................9 List of Equipment and Customer Information ......................10 Care and Maintenance ..............................11 Principle of Measurement ............................
  • Page 4 © PSI (Photon Systems Instruments), spol. s r. o. 11.4 Firmware Update ..............................50 GPS Module ................................53 12.1 GPS / AquaPen Operation ........................... 53 12.2 Data Download ..............................54 Warranty Terms and Conditions ..........................55 Troubleshooting and Customer Support ......................... 56...

  • Page 5: Information Before Using Aquapen Device

    © PSI (Photon Systems Instruments), spol. s r. o. 1 INFORMATION BEFORE USING AQUAPEN DEVICE Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide.

  • Page 6: Technical Specification

    © PSI (Photon Systems Instruments), spol. s r. o. 2 TECHNICAL SPECIFICATION Measured / Calculated Parameters: ‘, QY, NPQ 1, NPQ 2, NPQ 3, OJIP, LC 1, LC 2, LC 3, OD *, OD Saturating Pulse Illumination: Adjustable from 10 to 100 % (up to 3,000 µmol.m Actinic Illumination: Adjustable from 10 to 1,000 µmol.m...
  • Page 7 © PSI (Photon Systems Instruments), spol. s r. o. * Only in AquaPen-C AP 110-C. ** Windows is a registered trademark of Microsoft Corporation. Bluetooth Module Compliance Data:...

  • Page 8: General Information

    © PSI (Photon Systems Instruments), spol. s r. o. 3 GENERAL INFORMATION AquaPen (AP) is a lightweight, hand-held fluorometer intended for quick and reliable measurements of photosynthetic activity in algal, cyanobacterial or plant cell suspensions. The photosynthetic activity is derived from the chlorophyll fluorescence (ChlF) kinetics.

  • Page 9: Device Description

    © PSI (Photon Systems Instruments), spol. s r. o. DEVICE DESCRIPTION Fig. 1 Device description.

  • Page 10: List Of Equipment And Customer Information

    © PSI (Photon Systems Instruments), spol. s r. o. 4 LIST OF EQUIPMENT AND CUSTOMER INFORMATION Standard version of the AquaPen device package consists: • AquaPen-C AP 110-C or AquaPen-P AP 110-P • Carrying Case • 3 pieces of 4 ml volume plastic cuvette with stopper (AquaPen-C only) •...

  • Page 11: Care And Maintenance

    © PSI (Photon Systems Instruments), spol. s r. o. 5 CARE AND MAINTENANCE AquaPen-P AP 110-P • Never submerge the whole device in the liquid! • only the optical tip can be submerged! • Rinse the optical tip of the AquaPen-P in freshwater after each use.

  • Page 12: Principle Of Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 6 PRINCIPLE OF MEASUREMENT AquaPen is a fluorometer adapted for measurements of chlorophyll fluorescence parameters in liquid suspensions of algae, cyanobacteria and isolated plant cells . Two version of the AquaPen are available, the cuvette version (AP-C) and the probe version (AP-P).
  • Page 13 © PSI (Photon Systems Instruments), spol. s r. o. Measuring the optical density of growing cultures is a common method to quantify various important culture parameters like cell concentration, biomass production or changes in the cell morphology. The cuvette version of the AquaPen measures OD at two wavelengths 680 and 720nm.
  • Page 14 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 3 AquaPen-C AP 110-C Fig. 4 AquaPen-P AP 110-P...

  • Page 15: Getting Started

    © PSI (Photon Systems Instruments), spol. s r. o. 7 GETTING STARTED For more detailed information on steps of AquaPen operation please refer to chapter 8. The device is powered by built in Li-Ion battery. Ensure that the battery if fully charged by plugging it into a PC via USB cable or the AC outlet via USB adapter (not included) and the cable.
  • Page 16 © PSI (Photon Systems Instruments), spol. s r. o. Flash pulse setting The optimum value of Flash pulse can be identified during QY measurement as shown in Fig. 5 below. Before performing QY measurement it is recommended to set the pulse color according to culture used (blue for algae and red for cyanobacteria) and intensity of Super pulse to 70 %.
  • Page 17 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 6 OJIP curves - measurement performed with different intensities of Super pulse. Fig. 7 OJIP data - measurement performed with different intensities of Super pulse. The highest Fv/Fm value indicates the optimal...

  • Page 18: Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. Actinic pulse setting Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application. If Overflow is observed on display during measurement there are two options to resolve this problem - to dilute the sample or lower the intensity of pulse.
  • Page 19 © PSI (Photon Systems Instruments), spol. s r. o. • Press SET to start measurements. • AquaPen will measure the parameter. If a protocol was selected such as OJIP, LC or NPQ the display will only show the progress of the measurements in % but no data will be visible.

  • Page 20: Ojip Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.3 OJIP PROTOCOL The AquaPen device offers the protocol to capture rapid fluorescence transient – OJIP, which occurs during exposure of photosynthetic organisms to high irradiance. The FluorPen software enables data downloading to a PC and subsequent OJIP visualization of the analyzed data in a graphical and tabular format.

  • Page 21: Non-Photochemical Quenching (Npq) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.4 NON-PHOTOCHEMICAL QUENCHING (NPQ) PROTOCOLS The NPQ protocol is used to quantify photochemical and non-photochemical quenching. It should be performed with dark- adapted samples. The NPQ protocol starts with a measurement of minimal level of fluorescence F during a dark period.
  • Page 22 © PSI (Photon Systems Instruments), spol. s r. o. For more details please refer to: Oxborough K., Baker N.R. (1997): Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components: calculation of qP and F ’/F ’ without measuring F ’.
  • Page 23 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 8 NPQ Protocol.

  • Page 24: Light Curve (Lc) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.1.5 LIGHT CURVE (LC) PROTOCOLS The protocols called Light Curve (LC) were designed to acquire parameters for construction of Light Response Curve relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the sample exposed to a stepwise increase of light intensity.
  • Page 25 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 9 LC1 Protocol.
  • Page 26 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 10 LC2 Protocol.

  • Page 27: Optical Density Measurement (Aquapen-C Only)

    © PSI (Photon Systems Instruments), spol. s r. o. OPTICAL DENSITY MEASUREMENT (AQUAPEN-C ONLY) 7.2.1 CALIBRATION Calibration of the AquaPen is required before measuring OD. It can be done with either plain water or ideally, culture medium placed in a cuvette and the AquaPen. Calibration assures accurate OD measurements and it is recomended before every set of samples.

  • Page 28: Multiple Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. • Close the cover. • Turn the device on by pressing SET key for 1 sec • Select from the menu Measurement > OD680nm or OD720nm. • Press SET to start the measurement.

  • Page 29: Control Menu Tree

    © PSI (Photon Systems Instruments), spol. s r. o. 8 CONTROL MENU TREE The next few pages of this manual show the structure of the firmware menu on the AquaPen device, and explain in a schematic way the operation of the AquaPen. The schematic shows the Main Menu, first-level Sub-Menus and second- level Sub-Menus.
  • Page 30 © PSI (Photon Systems Instruments), spol. s r. o.
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  • Page 37: Usb Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 9 USB CONNECTION AquaPen comes with the USB cable that is required for charging of the Li-ion battery and can also be used for data transfer to the PC after completion of measurements. To connect the USB cable with the AquaPen device Follow the picture instructions below.

  • Page 38: Bluetooth Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 10 BLUETOOTH CONNECTION In addition to data transfer via USB the AquarPen may be connected to the software via Bluetooth for data transfer. Before setting up the Bluetooth connection between the AquaPen and the PC, ensure the following components are in place: 1.
  • Page 39 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 12 Start Bluetooth Application. 3. Opening the Add Bluetooth Device Application • Select: “Add a device” to start searching for the new Bluetooth device. Be sure that the AquaPen is in discoverable mode (see step 1).
  • Page 40 © PSI (Photon Systems Instruments), spol. s r. o. 5. Starting the Pairing Process This step is different for old and new version of the AquaPen, that are equipped with disparate Bluetooth module. Old version of AquaPen (AP-100) : Your Bluetooth Pairing Code is: 0000 •...
  • Page 41 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 17 Finishing. The Bluetooth pairing is now complete, and the next step is to open the program FluorPen 1.1 (included on the USB flash disk) For more information about FluorPen software see chapter 11.

  • Page 42: Fluorpen Software

    © PSI (Photon Systems Instruments), spol. s r. o. 11 FLUORPEN SOFTWARE 11.1 SOFT WARE INSTALLATIO N 1. Copy the FluorPen software provided on the USB flash disk to your computer and launch the FluorPen program. 2. To connect and recognize the AquaPen device in the FluorPen software, proceeds first with the registration of the FluorPen software (Fig.

  • Page 43: Menu And Icon Explanation

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 19 Connecting AquaPen with Software. 11.2 MENU AND ICON EXPLAN ATION 11.2.1 MAIN MENU MENU: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format.

  • Page 44: Menu Settings

    © PSI (Photon Systems Instruments), spol. s r. o. MENU: Setup Device ID Detects the connected device. Update Firmware Used for firmware updates. Settings Used for modification of the program settings. MENU: Help About Offers basic information about the program.

  • Page 45: Menu Online Control

    © PSI (Photon Systems Instruments), spol. s r. o. Data – GPS Visible This option is active only in older AquaPen version AP 100. In new version AP 110 the GPS data are automatically downloaded and paired with protocol measurements.
  • Page 46 © PSI (Photon Systems Instruments), spol. s r. o. Online Control – Time In this window the AquaPen time and date. One can also synchronize time of AquaPen device with computer time. This is essential for correct GPS data acquisition and therefore recommended Online Control –...

  • Page 47: Data Transfer And Visualization

    © PSI (Photon Systems Instruments), spol. s r. o. 11.3 DATA TRANSFER AND VISUALIZATION 1. Once kinetic protocols data (OJIP, NPQ, LC) have been collected with the AquaPen to visualize the data it needs to be downloaded to the PC first via FluorPen software. Before data transfer can occur a successful connection between the AquaPen and the PC needs to be established via USB cable or Bluetooth module (see chapter 9 and 10 for details).
  • Page 48 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 20 Example of Data Transfer and Visualization. 4. To visualize the data in the graph mode, click the Graph field in the bottom bar. 5. The selected set of data will be shown on the Graph (Fig. 21).
  • Page 49 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 21 Graphic visualization of experiment. 6. To export data from the FluorPen software select File > Export or Export icon. Select data type to export (Ft, QY, OJIP…) - Fig. 22.
  • Page 50 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 22 Export data.

  • Page 51: Firmware Update

    © PSI (Photon Systems Instruments), spol. s r. o. 11.4 FIRMWARE UPDATE All data in the AquaPen memory are erased during the firmware update! Before starting any firmware update, download all your data from the AquaPen memory to the computer! 1.
  • Page 52 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 25 Select .bxn file. 4. Finishing Upload • Select: OK to start uploading of the update (Fig. 26). Fig. 26 Data loss warning. • The bottom bar indicates the upload progress (Fig. 27).

  • Page 53: Gps Module

    © PSI (Photon Systems Instruments), spol. s r. o. 12 GPS MODULE The new versions of the AquaPen device (AP110) have integrated GPS module which can be turned on during the measurements. When GPS module is turned on the map coordinates will be automatically saved with all collected data and will be downloaded during data download.

  • Page 54: Data Download

    © PSI (Photon Systems Instruments), spol. s r. o. 12.2 DATA DOWNLOAD 1. Enabling Communication: • Switch on the computer and the AquaPen. Set your computer to AquaPen communication: enable Bluetooth or connect to USB port (see instructions on pg. 37).

  • Page 55: Warranty Terms And Conditions

    © PSI (Photon Systems Instruments), spol. s r. o. 13 WARRANTY TERMS AND CONDITIONS • This Limited Warranty applies only to the AquaPen device. It is valid for one year from the date of shipment. • If at any time within this warranty period the instrument does not function as warranted, return it and the manufacturer will repair or replace it at no charge.

  • Page 56: Troubleshooting And Customer Support

    © PSI (Photon Systems Instruments), spol. s r. o. 14 TROUBLESHOOTING AND CUSTOMER SUPPORT In case of problems with the AquaPen visit on our websites (http://psi.cz/support/faq) or contact customer support by email to support@psi.cz , or contact your local distributor.

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